당뇨 유발쥐에서 인동초의 섭취가 혈청지질과 혈당 및 항산화효소계에 미치는 영향

방미애,조영자,김현아 한국식생활문화학회 2002 韓國食生活文化學會誌 Vol.17 No.4

The purpose of this study was to investigate the effects of dietary Indongcho(L. japonica Thunb) powder on blood glucose, serum lipid levels and antioxidative anzymes in normal and streptozotocin(STZ)-induced diabetic rats. Four groups of rats(2-week-old inbred Sprague-Dawley male rats) were normal rats fed control diet(NC), diabetic rats fed control diet(DC), normal rats fed Indongcho powder diet(NI), and diabetic rats fed Indongcho powder diet(DI). Diabetes was induced by single injection of streptozotocin(60mg/kg BOW., i.p.). The animals were fed ad libium each of the experimental diet for 5 weeks. Food and water intakes were determined everyday. Blood glucose and serum total cholesterol levels were determined every week. After 5 weeks the animals were sacrificed and activities of antioxidant enzymes and lipid peroxidation products were determined in their liver and kidney homogenates. We also determined serum concentrations of total lipid(TL), total cholesterol(TC), triglycerides(TG) and HDL-cholesterol(HEL-C).Blood sugar and water intake were higher in decreased by dietary Indongcho intake, Body weight gain and FER(feed efficiency ration) were reduced by STZ treatment. But, Final body weight was recovered by Indongcho-contained diet. LHR(LDL-cholesterol/HDL-cholesterol) of the DI group was significantly lower than the other experimental groups(NC, NI and DE groups). The hepatic glucose 6-phosphatase(G6Pase) activity of the groups fed Indongcho diet(NI and DI group) was lower than the groups fed control diet(NC and DC group) and the G6Pase activity of NI group was recovered to the normal levels(p<0.05). However, The glutathione peroxidase(GPx) and glutathione reductase(GR) activities in liver and G6Pase activity in kidney were not statistically different between the control and diabetic control groups. Renal GST activity of the DI group was recovered by Indongcho intake. In conclusion, these results confirm oxidative stress in the liver and kidney of rats with STZ diabetes and antioxidative effect of Indongcho.

타액분비량이 증가하는 입체조의 효과와 만족도

방혜인(외 3명) 신라대학교 자연과학연구소 2012 自然科學論文集 Vol.22 No.-

Objectives: This study was performed to grasp the relation between the effect and the satisfaction of mouth gymnastics program on the promotion of oral function of students. We investigate the degree of digestion due to stress and the changes in salivary flow. We should investigate about the relationship of xerostomia according to the changes in salivary secretion. Methods: Total 79 students for two weeks, Silla University Dental Hygiene Department participated in this survey. Results: In the point of view of oral xerostama, people without drinking have lower symptom and action of xerpstama than those who drink three or four times a week. As the change rate of salivation amount is large, the degree of digestion, stress and xerpstama symptom and action is low. However the satisfication of oral exercise program is high. Conclusions: Adult should pay attention to oral health through oral exercise which increases the amount of salivation. Also Oral and total health should be increased through oral health education for the methodical oral health program

국내에서 분리된 canine parvovirus의 구조유전자 cloning과 염기서열 분석

박종현,송재영,이중복,현방훈,안수환,전무형 충남대학교 생물공학연구소 1993 생물공학연구지 Vol.3 No.-

In this study gene encoding structural proteins of a CPV isolate was cloned and saquenced to elucidate the molecular genetical properties of the canine parvoviruses isolated from the field. Six recombinant plasmids of pEP3, p1471, p2070, pEP069, pEP338 and p14711p were constructed from the map positions 22 to 98 of RF DNA to clone the VP1 and VP2 genes of CPV-V20. Sequentialy the gene comprising 3780 nucleotides were sequenced by dideoxy chain termination method. When nucleotide sequence of gene encoding the structural proteins of CPV-V20 was compared with those of other strains, CPV-N, CPV-d and CPV-780929 published previously. DNA homologies to CPV-V20 were 99.87% with CPV-NM, 99.73% with CPV-d, 96.85% with CPV-780929 AND 98.4% with FPLV-Carl, respectively. The DNA sequence data of CPV-V20 showed seven point mutations and also deletion of 135 nucleotides from the nucleotide position 4745 to 4879 located in the 3-noncoding region of CPV-N.

Pocine Adenovirus-3의 E1B Region의 鹽基序列 分析

朴鍾賢,宋載永,李重馥,玄芳勳,安東濬,車相昊,裵用泰,姜永源,Reddy, P S,全茂炯,安壽煥 충남대학교 생물공학연구소 1999 생물공학연구지 Vol.7 No.-

돼지 아데노바이러스(PAV-3). 6618주의 EIB region이 包含되어 있는 map unit 4.0에서 9.7까지의 유전자에 대한 1,984 bp의 염기서열을 決定하였으며, 이 結果를 알려진 여러 아데노바이러스 유전자와 비교하여 다음과 같은 결과를 얻었다. 1. PAV-3의 EIB유전자는 10개의 ORF로 구성되어 있으며, 그 중 아데노바이러스의 단백질과 유사성이 있는 것은 ORF1, ORF2 및 ORF3이었다. ORF1은 Ad41의 19kd 와 BAV-2에서의 EIB ORF2에서의 아미노산의 一致率은 각각 32%와 31%이었다. 2. ORF2는 Ad2 55kd protein과 tupaia adenovirus 44kd protein가 각각 34%로 아미노산 一致率이 가장 높았으며, Ad41의 52kd protein. BAV-3의 EIB ORF3에서도 33%의 一致率을 보였다. 3. ORF1은 61-666 uncleotide (606 bp), ORF 2에서는 429-1,850 uncleotide (1,422 bp)의 부위로 각각 202, 474 a.a로 構成되었으며, 예상되는 분자량은 20 kd와 52 kd이었다. 4. ORF3는 hexon-associated pIX유전자로 추정되며 내부에 1개의 polyadenylation signal(ATAAA)이 1938-1942 uncleotide에 위치하였으며, 이 부위는 TATA box (1937-1942 uncleotide)와 중복되어 존재하였다. Porcine adenovirus type 3 (PAV-3) does not cause severe infection in pigs. Adenovirus has been suggestive of live vaccine vector carrying foreign gene. One of insertion regions is delayed early (EIB) region. However, EIB region of PAV-3 has not been molecularly characterized to date. Nucleotide sequence of EIB of PAV-3 was determined. The EIB region was composed of 1,984 bp and located between 4.0 and 9.7 map units. Three potential open reading frames(ORFs) with low level of homology to other adenoviruses and a polyadenylation signal were identified in the rightward direction of genome. The nucleotide and the predicted amino acid sequences of EIB were compared to those of human and animal adenoviruses. One of the three potential ORFs. ORF1 encoded a polypeptide homologous to bovine adenovirus type 2(BAV-2) ORF2 and human adenovirus type 41(Ad41) 19 kd protein. ORF2 encoded a polypeptide homologous to human adenovirus type 2(Ad2) 55 kd protein, bovine adenovirus type 3(BAV-3) ORF3 and porcine adenovirus type 4(PAV-4) ORF2. The predicted protein of ORF1 had homology to those of Ad41 and BAV-2 with 32 and 31% respectively, whereas the deduced protein of ORF2 had homology to those of Ad2. BAV-3 and PAV-4 with 34, 33 and 29%, respectively.

유도선수의 여가 참여형태에 따른 만족도 분석

조방현,김영환,이철원 연세대학교 체육연구소 2002 체육연구논문집 Vol.9 No.1

본 연구는 엘리트 유도선수들이 과연 여가시간을 어떠한 방법으로 활용하는지에 대한 의문을 바탕으로 문제해결을 시도하였으며 이를 통하여 여가 참여 형태와 여가 만족에 대한 측면을 분석하였다. 이러한 과정을 통하여 유도선수들의 여가활동에 대한 의식 및 만족도를 파악할 수 있었으며 또한 유도선수들이 효율적인 여가시간을 가질 수 있는 방안을 고안하고자 하였다. 구체적으로 이와 같은 연구 목적을 달성하기 위해 본 연구에서는 중, 고등학교와 대학교에 재학 중인 유도선수들을 대상으로 설문조사를 실시하여 여러가지 각도, 참여정도, 여가경비, 여가제약, 여가동기, 선호여가형태 등을 조사하였고, 아울러 여가만족도를 심리적 요인, 휴식적 요인, 교육적 요인, 사회적 요인, 환경적 요인, 그리고 생리적 요인으로 구분하여 교차분석과 분산분석을 실시하였다. 사후검증은 Turkey(표준화검정)을 실시하여 분석하였다. 이러한 연구 과정을 통하여 다음과 같은 결론을 얻었다. 첫째, 유도선수들의 여가참여정도에 따라 여가만족도는 교육적 요인, 사회적 요인, 그리고 환경적 요인에서 유의한 차이를 보였다. 둘째, 유도선수들의 선호여가형태에 따라 여가만족도는 교육적 요인과 생리적 요인에서 유의한 차이를 보였다. 셋째, 유도선수들의 여가제약에 따라 여가만족도는 유의한 차이가 없었다. 넷째, 유도선수들의 여가경비에 따라 여가만족도는 사회적 요인에서 유의한 차이를 보였다. 마지막으로 유도선수들의 여가동기에 따라 여가만족도는 유의한 차이가 없었다. The purpose of this study was to explain the judo players' leisure time and satisfaction through analyzing their leisure participation patterns and satisfaction. This also pursuits effective understanding of the leisure time and relationship between training and leisure time of Judo players. For the purpose of the study, the research participants (315 out of 355) were conducted from groups of middle school, high school and college judo players with questions of perception, frequency of participation, involved expense, limitations, motivation, and preferences toward leisure activities. These variables were used to quantify the tendency of leisure satisfaction. In the data analysis, frequency test, Chi-square test, and One-way ANOVA were executed. Furthermore the data was grouped into psychological, resting condition, educational, social , environmental, and physical factors to analyze the degree and tendency of leisure satisfaction. The results were shown as follows: 1. There were shown significant differences from the leisure participation of Judo players about leisure satisfaction on the educational, social, and environment factors. 2. There were indicated as significant differences of the preferred patterns of Judo players about leisure satisfaction on educational and physiological factors. 3. There were not shown significant differences leisure constrains of Judo players about leisure satisfaction. 4. There were shown significant differences about the expenses for leisure of Judo players about leisure satisfaction on the social factor. 5. There were not suggested significant differences of the leisure motive of Judo players about leisure satisfaction.

Baculovirus를 利用한 Canine Parvovirus VP2蛋自質의 發現

朴鍾賢,宋載永,玄芳動,安動濬,姜永源,全茂炯,安壽煥 충남대학교 생물공학연구소 1998 생물공학연구지 Vol.6 No.-

국내에서 分離된 개파보바이러스주(V20주)의 VP2 遺傳子를 baculovirus system을 이용하여 발현시켜 다음의 結果를 얻었다. 1. 개파보바이러스의 VP2 유전자를 PCR에 의해 增幅하여 1755bp의 VP2遺傳子를 pUC19에 클로닝하여, 클로닝된 遺傳子를 polyhedrin promoter를 가지는 baculovirus expression vector인 pVL1393에 옮겨 VP2 발현벡터인 pVL1393-VP2를 얻을 수 있었다. 2. pVL1393-VP2 plasmid와 baculovirus DNA와의 homologous recombination에 의해 재조합바이러스인 VP2-BV를 얻을 수 있었으며, 그 발현효율은 2.000-5.000 HAU/0.05 ml이었다. 3. 免疫沈澱法에 의해 발현된 단백질은 개파보바이러스의 VP2단백질과 유사한 64 kb에 달하는 것이었으며, 血球凝集能을 지니고 있었다 4. 血球凝集能을 지닌 蛋白質이 여러 陽性血淸에 있어서 抗體수준을 測定할 수 있는지 개파보바이러스항원과의 相關性을 比較한 바 0.94 (n=125. p<0.01)의 相關係數를 보였다 5. 발현 VP2白은 virus-like particles를 形成하였으며, 그 크기로는 개파보바이러스와 비슷한 25 ㎚의 크기를 갖았다. Canine parvovirus(CPV) is a member of autonomous replicating parvoviruses and is aetiologically associated with enteritis and mycoarditis in puppies. The capsids of CPV are composed of three structural proteins: VP1, VP2 and VP3. The VP2 protein is the major component of capsid. The VP2 gene of a canine parvovirus. V20 strain isolated in Korea was cloned into baculovirus expression vector, and subsequently the VP2 protein was expressed by a recombinant baculovirus under the control of polyhedrin promoter. The recombinant VP2 protein expressed in Spodoptera frugiperda 9(Sf9) cells was detected by haemagglutination(HA) test and immunofluorescent antibody assay. Molecular weight of the recombinant VP2 protein expressed was estimated as 64Kd when tested by immunoprecipitation test using anti-CPV monoclonal antibody. In haemagglutination inhibition(Ⅲ) test. 8 HA units of the recombinant VP2 protein antigen was successfully utilized to determine a level of antibody against CPV in various positive sera. The recombinant VP2 protein showed also the capability to form virus like particles similar in size and appearance to the CPV virions.

생활체육 축구 동호인의 운동 상해에 관한 연구

강경빈,김갑선,방현창 목포대학교 스포츠산업 연구소 2006 스포츠 산업연구소 논문집 Vol.5 No.-

The purpose of the study was to investigate time and causes of the wound during physical exercise, emergency measures and the state after the wound, etc that the amateurs interested in soccer sports might experience and to supply material for wound prevention and body management. The subjects were 499 men who joined the activities of the association of amateurs interested in soccer sports in Jeonbuk in 2005. The questionnaire survey was used. The reliability on the questionnaires was good (Cronbach a=.6895-8520). Not only Crosstabs but also χ2 (Chi-square) test was conducted to investigate demographical features of the ones interested in soccer sports, the purpose of participation and difference between the wound during physical exercise and to have significant difference (P<.05). The findings were as follows; Firstly, the purpose of participation of the amateurs interested in soccer sports depending upon demographical variables was health maintenance (42.7%) and good human relations (38.9%), so that they participated in soccer sports to be healthy and establish good human relations. Secondly, the wound occurred in order of in spring (52.3%), in autumn (35.5%) and in summer (9.4%), and age, activity experience and training experience from experts, sports game rules and occupation, etc had significant difference at the time of the wound during physical exercise (P<.001). The place where the wound occurred was in order of bare ground (94.8%) and lawn (5.2%). and training experience from experts and sports game rules had significant difference (P<.001). And, the time that the wound occurred was in order of during playing (61.7%) and during exercise (38.3%), and the sports game rules and occupation variables had significant difference (P<.001). At occurrence of the wound, emergency measure was used in order of no action (66.5%), medicines (30.7%) and cold-pack (2.81%). The place where medical treatment was made was in order of herb medicine clinic (53.3%), hospitals (23.2%) and home (20.2%). Thirdly, the difference during physical exercise varied depending upon variables of participating purposes. The season of the wound occurrence had significant difference (P<.001), and the wound occurrence time had significant difference between human relations, health maintenance, stress relief and hobby activities, etc (P<.001). The wound occurrence place in accordance with the purpose of sports participation had no significant difference, while the wound occurrence time had significant difference (P<.001). The wound was cured in order of by himself (44.0%) and emergency treatment expert (36.0%), and emergency measure immediately after the wound occurrence was made in order of medicines (61.2%) and no action (37.3). It took two weeks or less (52.1%) and one week or less (32.2%) to cure the wound completely, and one week and three weeks or less (34.0%) and two weeks or less (30.2%) to maintain health. The activity state after medical treatment was in order of continuously injured at the wound area (88.1%) and being worse than before being wound (11.9%). Treatment time of the wound and activity state after treatment had significant difference (P<.001).

제조합 대장균에서 과발현된 Citrobacter freundii KCTC2006 유래의 β-Tyrosinase를 이용한 3,4-Dihydroxyphenyl-L-alanine의 생산

이승구,노현수,홍승표,이규종,왕지원,태동년,엄기남,방상구,김영준,성문희 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.1

재조합 대장균에서 대량발현 시킨 Citrobacter freundii KCTC 2006 유래이 효소 β-tyrosinase를 이용하여 pyrocatechol, sodium pyruvate, ammonium acetate로부터 3,4-dihydroxy phenyl-L-alanine을 생산하기 위한 연구를 수행하였다. 이 효소반응에 적합한 온도 및 pH 조건은 각각 18℃와 8.5로 결정되었고, 반응액 중의 ammonium acetate와 sodium pyruvate의 농도는 각각 300 mM, 50 mM 이상으로 조절하는 것이 적합하였다. Pyrocatechol의 경우는 20 mM에서 가장 높은 반응성을 나타냈으나, 기질을 반복적으로 첨가하며 장시간 동안 효소반응을 수행하는 경우에는 pyrocatechol의 고갈을 피하기 위하여, 20 mM에서 50 mM 사이로 조절하였다. 한편, 반응액 중에 ethanol을 10% 첨가한 경우에는 반응속도가약 20% 증가하였다. 이상과 같은 효소반응특성에 기초하여 조제한 기질용액에 β-tyrosinase를 1 unit/㎖ 농도로 가하고, pyrocatechol과 pyruvate가 고갈되지 않도록 간헐적으로 첨가하면서 효소반응을 수행한 결과, 24시간 만에 85.2%의 수율로 31.6g/ℓ의 3,4-dihydroxyphenyl-L-alanine를 생산할 수 있었다. By using the β-tyrosinase of Citrobacter freundii KCTC2006, which was cloned and overexpressed in Escherichia coli, 3,4-dihydroxy phenyl-L-alanine (L-DOPA) was synthesized efficiently from pyrocatechol, sodium pyruvate, and ammonium acetate. Optimal temperature and pH for the reaction were determined to be about 18℃ and 8.5, respectively. The effects of substrate concentrations were also examined at different concentrations of ammonium acetate, sodium pyruvate, and pyrocatechol. Ammonium acetate and sodium pyruvate increased the reaction rate until the concentrations reached to 300 mM and 50 mM, respectively. Although pyrocatechol showed the optimal concentration at 20 mM, it was controlled between 20 mM and 50 mM to avoid the depletion of substrate during the enzymatic synthesis. Based on above results, a reaction medium for the production of L-DOPA was prepared and incubated with 1 unit/㎖ of β-tyrosinase. Pyrocatechol and sodium pyruvate was added to the reaction solution intermittently to avoid the substrate depletion during the enzymatic reaction. After 24 hour of reaction, 31.6 g/ℓ L-DOPA was accumulated in the reaction solution as soluble and precipitated ones and the conversion yield was about 85.2%.

식이 중 인동초를 섭취한 당뇨유발 흰쥐의 혈당 및 지질대사

조영자,김현아,방미애 목포대학교 생활과학연구소 2000 생활과학논집 Vol.3 No.-

The purpose of this study was to invesgate the effect of L. japonica tumbo(P) on blood glucose level and lipid metabolism in streptozotocin(STZ) induced diabetic rats. Male Sprague-Dawley rats weighing(180~200g)were divided into four groups: control(C), STZ-control(CD), and two experimental groups(P and PD). Diabetes mellitus were induced by single injection of the streptozotocin at tue dose of 60mg/kg B.W. The experimental groups were fed a AIN-76 recomendation diet mixed with the P powder(5%/kg diet). The diet and water were intake determined everyday. The blood glucose level was determined everyweek, and the serum cholesterol was determined every two weeks. After 5 weeks, the rats sacrificed and the blood glucose, serum cholesterol. triglyceride, HDL-cholesterol concentrations were determined. LDL-cholesterol concentrations was calculated by eqation. Diabetic rats showed the lower weight gain compared to the normal rats. The serum glucose level of PD was not lower than C group. However, the serum cholesterol, triglyceride and HDL-cholesterol level of CDI and PD group were heigher than C group. Diabetic rats showed that the hepartic glutathione reductase(GR) was sighificantly heigher than C group, but malondialdehyde level was significantly decreased. P supplymentation was reduced the hepartic malondialdehyde(MDA) level. The glutathione reductase(GR) and HDL-cholestrol level were increased in the diabetic experimental groups compared C group. In comculusion, showing the possibility of preventive and therpeutic use of the wild edible plant(Indong- Cho) to the diabetes mellitus.

자색고구마가 사염화탄소 투여에 의한 흰쥐의 간손상 보호에 미치는 영향

조영자,김현아,방미애,오용비,정병춘,문윤호,정우진 한국식생활문화학회 2003 韓國食生活文化學會誌 Vol.18 No.3

The purpose of this study was to investigate the effects of dietary purple sweet potato(lpomoea batatas) powder on serum lipid levels and antioxidative enzymes in normal and pretective effect on hepatotoxicity rats induced by carbon tetrachlolide. Four groups of rats (3-week-old inbred Sprague-Dawley male rats) were normal rats fed control diet(C)_(1) induced hepatotoxicity rats fed control diet(EC)_(1) normal rats fed purple sweet potato diet(P), and induced hepatotoxicity rats fed purple potato sweet diet(EP). Rats were induced by single injection of 50% carbon tetrachloide(0.1 mL/100 g B.W., i.p.). The rats were fed ad libitum each of the experimental diet for 5 weeks. After 5 weeks the rats were sacrificed and activities of antioxidant enzymes and lipid peroxidation products were determined in their liver homogenates. But serum concentrations of lipid was not significant in all groups. Serum alanine aminotransferase(ALT/GPT) and aspartate aminotransferase(ALT/GPT)of the EC and EP groups were heigher than the C and P groups. The hepatic glucose 6-phosphatase(G9Pase) activity of the group fed purple potato diet(P) was lower than the other groups(p<0.05). However, The glutathione peroxidase(GPx) activities was not statistically different between the groups. Renal glutathione S-transferase(GST) activity of the EC and EP groups were lower than the C and P groups(p<0.05). In conclusion, these results suggest that purple sweet potato is believed to be possible protective effect on hepatotoxicity rats induced by carbon tetrachlolide.