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      • SCIESCOPUSKCI등재

        Validation of Methods for Isolation and Culture of Alpaca Melanocytes: A Novel Tool for In vitro Studies of Mechanisms Controlling Coat Color

        Bai, Rui,Sen, Aritro,Yu, Zhihui,Yang, Gang,Wang, Haidong,Fan, Ruiwen,Lv, Lihua,Lee, Kyung-Bon,Smith, George W,Dong, Changsheng Asian Australasian Association of Animal Productio 2010 Animal Bioscience Vol.23 No.4

        The objective of the present studies was to develop and validate a system for isolation, purification and extended culture of pigment-producing cells in alpaca skin (melanocytes) responsible for coat color and to determine the effect of alpha melanocyte stimulating hormone treatment on mRNA expression for the melanocortin 1 receptor, a key gene involved in coat color regulation in other species. Skin punch biopsies were harvested from the dorsal region of 1-3 yr old alpacas and three different enzyme digestion methods were evaluated for effects on yield of viable cells and attachment in vitro. Greatest cell yields and attachment were obtained following dispersion with dispase II relative to trypsin and trypsin-EDTA treatment. Culture of cells in medium supplemented with basic fibroblast growth factor, bovine pituitary extract, hydrocortisone, insulin, 12-O-tetradecanolphorbol-13-acetate and cholera toxin yielded highly pure populations of melanocytes by passage 3 as confirmed by detection of tyrosinase activity and immunocytochemical localization of melanocyte markers including tyrosinase, S-100 and micropthalmia-associated transcription factor. Abundance of mRNA for tyrosinase, a key enzyme in melanocyte pigment production, was maintained through 10 passages showing preservation of melanocyte phenotypic characteristics with extended culture. To determine hormonal responsiveness of cultured melanocytes and investigate regulation of melanocortin 1 receptor expression, cultured melanocytes were treated with increasing concentrations of ${\alpha}$-melanocyte stimulating hormone. Treatment with ${\alpha}$-melanocyte stimulating hormone increased melanocortin receptor 1 mRNA in a dose dependent fashion. The results demonstrated culture of pure populations of alpaca melanocytes to 10 passages and illustrate the potential utility of such cells for studies of intrinsic and extrinsic regulation of genes controlling pigmentation and coat color in fiber-producing species.

      • KCI등재

        Synthesis of carbon/P-zeolite composites from coal gasification fine slag and studies on adsorption characteristics for methylene blue

        Shu Rui,Bai Jiaming,Guo Feiqiang,Mao Songbo,Qiao Qixia,Dong Kaiming,Qian Lin,Bai Yonghui 한국화학공학회 2023 Korean Journal of Chemical Engineering Vol.40 No.7

        Carbon/P-zeolite composites (CPZCs) were synthesized by high-temperature activation followed by hydrothermal crystallization using waste coal gasification fine slag as the raw material and NaOH as the activator. Methylene blue was selected as a dye pollutant for the adsorption experiment to investigate the adsorption property of the carbon/P-zeolite composites. It was found that the structure of CPZCs mainly depended on the mass ratio of NaOH to the coal gasification slag. At a proper NaOH to the coal gasification fine slag mass ratio of 1.0, the as-synthesized CPZC-1.0 exhibited a typical Na-P zeolite structure with a relatively high specific surface area of 200 m2/g and pore volume of 0.21 cm3/g. CPZC-1.0 showed excellent performance on methylene blue adsorption, with the adsorption capacity reaching 130 mg/g with removal efficiency of 81.6% at 303 K for the 100 mg/L initial methylene blue solution. The calculation results of adsorption thermodynamics indicated that the adsorption process to methylene blue by CPZCs was a spontaneous and entropy-driven endothermic process. Based on the above research, it is speculated that the adsorption mechanism of CPZCs to methylene blue might be due to the van der Waals force, intermolecular hydrogen bond and electrostatic attraction between Na-P zeolite and methylene blue.

      • KCI등재

        中韓兩國文化交流的發展與展望

        白銳 中韓人文科學硏究會 2001 한중인문학연구 Vol.7 No.-

        본 논문은 네 개 부분으로 나누어 있는데 즉 (1) 中韓文化交流의 새로운 발전 (2)中韓文化交流에 대한 전망 (3)中韓文化交流 중의 몇 가지 문제 (4)맺음말 등이다. 논문에서는 中韓 두 나라가 최근 십 년이래 문화 방면의 내왕을 서술하면서 이 교류가 두 나라 정부로부터 유력한 지지를 받아 성과적으로 진행했다는 것을 지적하였다. 아울러 두 나라 民間文化交流가 활발히 전개하고 있다는 것도 서술하였다. 논문에서는 兩國問의 文化交流를 통하여 이미 얻은 성과를 중점적으로 고찰하면서 두 나라간의 깊은 理解와 두 나라 國民사이의 親善과 友誼를 강화 발전하는데 이바지했다고 언급하며 찬양하였다. 韓民族은 커다란 抱負를 가지고 굳센 의지를 가지며 깊은 정을 귀중히 여기는 민족임으로 兩國 文化交流에 대하거나 양국의 親善關係를 발전시키는데 대하거나 언제나 적극적 태도를 취하고 있다. 오랜 역사적 관계로 中韓兩國의 文化關係도 매우 좋게 유지하여왔으며 兩國文化의 同質性도 충분히 나타나고 있다. 바로 그러기에 兩國국민들은 찬란한 東方文明을 共同으로 創造하는데 큰 기여를 하였다. 또한 韓民族은 공부와 연구에 뛰어나고 남의 長點을 수용하며 자기의 民族文化를 固守發展하는 것을 중요한 과업으로 간주하고 있다. 논문은 大量적 實例로 大衆性을 띤 文化交流의 業積을 진술하였다. 두 나라 觀光事業의 전개, 經濟상의 相互 協助, 大量적 유학생들의 파견을 통하여 문화교류의 발전을 推動하였다고 진술하였다. 두 나라 靑少年들의 交流도 깊은 의의를 가진다고 본다. 최근 시기에 韓國분들은 政治, 外交, 經濟舞臺에서 세상에 놀라운 表現들을 보여주었다. 日本이 二次大戰의 歷史를 歪曲하고 자기의 侵略本質을 은폐하려는 敎科書問題에 대하여 果敢하고 決斷하는 原則적 態度를 취하여 아세아 金融危機를 극복하고 자기의 경제를 회복 발전시키는 途上에서 고상한 愛國精神과 不屈不撓의 투지를 발휘하였다. 이와 같은 韓國精神은 세상 사람들로 하여금 敬慕하게 하였다. 우리는 韓國國民에 대하여 숭고한 경의를 표하는 理由를 충분히 가지고 있다. 논문에서 이상의 내용도 포함시키는 목적은 文化交流중에서 그 정신교류를 忽視할 수 없다는 것을 말씀드리려고 하는 데에 있다. 精神적 作用은 人間의 精神적힘을 喚起할 수 있는 것이다. 또한 그 범주의 내용의 교류는 역시 깊은 각도로부터 두 나라의 호상 이해와 정을 맺는 데 작용한다. 中韓交流의 課題가 매우 많은 것이다. 두 나라의 人員의 往來를 강화하고 學術硏究活動을 적극적으로 전개하며 상대방의 우수한 哲學, 文化言語, 宗敎, 藝術, 지어 좋은 영화와 텔레비전 작품 등등을 대폭적으로 소개하는 것도 매우 필요하다고 본다. 靑少年들의 往來 및 共同關心事로 되는 문제의 연구는 역시 중한 문화교류의 중요한 내용으로 된다. 우리는 한국의 企業文化, 文化政策 및 文化法規에 대하여도 흥미를 가지고 있다. 앞으로 이런 방면의 교류도 활발하게 전개하는 것을 희망하고 있다. 中韓文化交流의 展望은 매우 좋은 것이다. 중한 兩國問의 政治, 經濟 發展에 따라 兩國 文化交流는 휘황한 빛으로 두 나라 前進의 길을 눈부시게 비추어 줄 것을 확신하는 바이다.

      • KCI등재

        Emergence of virulent pseudorabies virus infection in Northern China

        Rui Wu,Chaoyong Bai,Jinzhong Sun,Shengke Chang,Xuke Zhang 대한수의학회 2013 JOURNAL OF VETERINARY SCIENCE Vol.14 No.3

        Our investigation was conducted in order to verify a recent severe epidemic at several swine farms in northern China that indicated a newly emerging disease. Evidence confirmed that the epidemic was caused by a virulent Pseudorabies virus infection in swine herds.

      • KCI등재

        Microstructure and tribological properties of Stellite 12 coating by laser cladding on 304 stainless steel surface

        Rui Wang,Changyao Ouyang,Yanling Li,Chunjiang Zhao,Qiaofeng Bai 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.8

        In the present study, the Stellite 12 coating was prepared on 304 stainless steel using laser cladding technology. The phase composition, microstructure, and friction behavior of the cladding layer under the four loads were analyzed. The results showed severe lattice distortion in the coating. As the temperature gradient and solidification rate were diverse in each region, the upper, middle, and lower sections of the coating layer exhibited various microstructure morphologies. The intergranular part of the cladding layer was mainly the eutectic structure, and the inner crystal was mainly Co solid solution. Spalling and adhesive phenomena appeared at the bottom of the specimen when the action was under a load of 90 N and 150 N. The average wear of the cladding layer improved with the increase in load, the shear stress and temperature in the contact zone were enhanced distinctly, and the specimen became more prone to plastic deformation and wear intensification.

      • KCI등재

        Does Early Endoscopy Affect the Clinical Outcomes of Patients with Acute Nonvariceal Upper Gastrointestinal Bleeding? A Systematic Review and Meta-Analysis

        Bai Liyi,Jiang Wei,Cheng Rui,Dang Yan,Min Li,Zhang Shutian 거트앤리버 소화기연관학회협의회 2023 Gut and Liver Vol.17 No.4

        Background/Aims: In patients with acute nonvariceal upper gastrointestinal bleeding (ANVUGIB), the optimal timing of endoscopy is still a matter of dispute. We conducted a systematic review and meta-analysis to determine the clinical benefit of early endoscopy. Methods: A literature search of the MEDLINE, Embase, and Cochrane databases was conducted to identify publications from inception to March 1, 2022. Eligible studies included observational cohort studies and randomized controlled trials that reported clinical outcomes of endoscopy in patients with ANVUGIB. ANVUGIB patients who underwent endoscopy within 24 hours of admission were considered to have had an early endoscopy. The primary outcome was the mortality rate in ANVUGIB patients who had early or nonearly endoscopy. Results: The final analysis included five randomized controlled studies (RCTs) and 20 observational studies from the 1,206 identified articles. The mortality rate was not significantly reduced among patients who received endoscopy performed within 24 hours, whether in cohort studies nor in RCTs. For subgroup analysis, a higher mortality rate was found only among patients who received very early endoscopy within 12 hours (odds ratio, 1.66; p<0.001, I2=0) in cohort studies. No significant difference in mortality rates was found among patients at high risk of bleeding who received early versus nonearly endoscopy. Conclusions: Early endoscopy within 24 hours does not appear to significantly reduce the mortality rates of patients with ANVUGIB. Further well-designed studies are warranted to address if very early endoscopy within 12 hours can provide a clinical benefit for patients at high risk of bleeding.

      • KCI등재

        Thermal Stability of GH4065A Superalloy Based on Microstructural and Performance Evolution Following Long-Term Aging

        Yingbo Bai,Rui Zhang,Chuanyong Cui,Yizhou Zhou,Xiaofeng Sun 대한금속·재료학회 2024 METALS AND MATERIALS International Vol.30 No.2

        This study investigates the thermal stability of a GH4065A superalloy at intermediate temperatures by conducting long-termaging treatments for 100–6000 h at 700–800 °C. Secondary γ′-phase coarsening and intergranular M6Ccarbide precipitationoccurred during aging. The activation energy for secondary γ′-phase coarsening was 259.89 ± 25.45 kJ mol−1, suggestingthat this process was predominantly controlled by elemental diffusion. The tensile strength at a testing temperature of 700 °Cwas stably above 1200 MPa for the alloys aged at 700 °C but continuously declined as the aging temperature increased, witha direct correlation identified between the secondary γ′-phase coarsening and strength reduction upon aging. Increasing theaging temperature transitioned the deformation mechanism from Orowan bowing around the γ′ phase to dislocation slipthrough the broadened matrix channels, leading to decreased strength. Long-term aging effectively eliminated intermediatetemperatureintergranular brittle fracture. The elongation to failure at a testing temperature of 700 °C increased by approximately35% after aging at 800 °C for 6000 h, attributed to grain-boundary diffusion of W and Mo and intragranular softening,which improved the deformation coordination ability at the grain boundaries. Finally, the precipitation of intergranular M6Cparticles after prolonged aging promoted plastic deformation via micropore aggregation.

      • GIS based Visual Expression of Marine Numerical Simulation- Flow Field and Silt Sediment as Example

        Wang Rui-Fu,Chen Li-li,Bai Tao,Wang Fang-zheng 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.1

        Numerical simulation has been widely applied in ocean engineering owing to its low cost, short cycle and wide application range. However, post-processing software of numerical simulation doesn’t support production specification of marine engineering and other industries, and integration production is not flexible. According to particularity of numerical simulation and user requirements, this paper took GIS as the solution to achieve dynamic visualization of numerical simulation and one-click generation of business products. Firstly, based on its characteristics, convert marine numerical simulation data into vector field represented by arrows or scalar field represented by grid. Secondly, GIS based spatial display and analysis technology is used to realize the visualization expression of marine numerical simulation data. In order to improve the effect of display and expression, we achieve rendering through symbol and color stretching mapping. Finally, we output numerical thematic map through custom map range and great map decoration function.

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