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박정배,성낙일,이준엽,허인경,김승민,남지형,이용국,최재혁,백진호,장태정,이종임,박건욱 동국대학교 의학연구소 2006 東國醫學 Vol.13 No.2
백화사설초는 한국과 중국에서 항암제로 이용되어온 약초이다. 이러한 백화사설초의 세포자멸사를 통한 항암 효과를 증명하기 위해 위암세포주인 SNU-719, 601, 668 각각에 백화사설초 메탄올 추출물로써 실험을 시행 하였다. 위암세포주 각각에 메탄올 추출물을 다양한 농도와 다양한 시간에 따라 실험 처치하여 세포 생존도를 측정하였다. 그리고 세포자멸사를 증명하기 위해 유세포분석을 통하여 subG1에 속하는 세포의 수를 계수하였고 Western blot 검사를 통해 caspase-3 enzyme의 발현을 조사하였다. SNU-719, 601 세포주에서는 메탄올추출물의 항암효과가 나타나지 않았고, SNU-668 세포주에서 항암효과가 나타났는데 메탄올 추출물 200과 400 ㎍/ml에서 농도에 비례하여 세포 생존도가 유의하게 감소하였다(0 ㎍/ml : 94.3土0.9%, 1 ㎍/ml : 95.8土0.8%, 5 ㎍/ml : 93.5土1.6%, 25 ㎍/ml : 95.3土2.0%, 50 ㎍/ml : 93.3土2.5%, 100 ㎍/ml : 93.5士3.8%, 200 ㎍/ml : 83.9土4.3%, 400 ㎍/ml : 72.8土8.4%). 100과 200 ㎍/ml, 200과 400 ㎍/ml 사이의 p-value는 각각 0.013, 0.005이었다. 그리고 메탄올 추출물 400 ㎍/ml을 SNU-668 세포주에 처리한 후 시간에 따른 세포 생존도를 조사한 결과 역시 시간의 증가에 따라 유의하게 감소하였다(0 hr. : 98.8土1.1%, 6 hr. ; 83.2土8.6%, 24 hr. : 37.6土8.3%, 48 hr. : 22.2土11.2%, p=0.019). 유세포분석상 subG1에 속하는 세포의 수는 농도와 시간에 비례하여 증가하였고 Western blot 검사상 caspase-3 proenzyme의 발현도 농도와 시간에 비례하여 감소하였다. 본 연구로 백화사설초의 메탄올 추출물이 위암세포주 SNU-668 세포자멸사에 효과가 있음이 증명되었다. 향후 백화사설초 메탄올 추출물의 성분분석과 세포자멸사 신호전달체계에 대한 연구를 통해 위암에 대한 새로운 항암물질 개발이 가능할 것으로 생각된다. Hedyotis diffusa has been used for cancer treatment in Korea and China. For the evaluation of anti-tumor activity through apoptosis, our experiments were performed with methanol extracts of Hedyotis diffusa on gastric cancer cell lines such as SNU-719, 601, 668. The cells were treated with various concentrations and periods of methanol extracts. Then, the cell viability was measured by trypan blue dye exclusion test. For the evaluation of apoptosis, we calculated the population of subG1 cells by the flow cytometry method, and examined the expression of caspase-3 by Western blot analysis. In SNU-719 and 601 cell lines, the anti-tumor activity of methanol extracts were not shown. However in SNU-668 cell lines, the anti-tumor activity was detected. The cell viability of SNU-668 was significantly decreased in a dose-dependent manner at 200 and 400 ㎍/ml of methanol extracts (0 ㎍/ml : 94.3±0.9%, 1 ㎍/ml : 95.8±0.8%, 5 ㎍/ml : 93.5±1.6%, 25 ㎍/ml : 95.3±2.0%, 50 ㎍/ml : 93.3±2.5%, 100 ㎍/ml : 93.5±3.8%, 200 ㎍/ml : 83.9±4.3%, 400 ㎍/ml : 72.8±8.4%). The p-values were 0.013 and 0.005 in between 100 ㎍/ml and 200 ㎍/ml, and 200 ㎍/ml and 400 ㎍/ml, respectively. The cell viability of SNU-668 was significantly decreased in a time-dependent manner as well (0 hr. : 98.8±1.1%, 6 hr. 83.2±8.6%, 24 hr. : 37.6±8.3%, 48 hr. : 22.2±11.2%, p=0.019). In the flow cytometry, the number of cells of subG1 population were increased in both dose-and time-dependent manners. In the Western blot analysis, caspase-3 proenzyme levels were decreased in both dose- and time-dependent manners as well. In conclusion, this study shows that methanol extracts of Hedyotis diffusa are effective on the apoptosis of SNU-668 gastric cancer cells. Therefore, through the analysis of components and the study of apoptotic signaling pathways of methanol extracts of Hedyotis diffusa, we could make a new drugs for the treatment of gastric cancer.
HCV core protein promotes liver fibrogenesis via up-regulation of CTGF with TGF-b1
Ju Yeop Shin,Wonhee Hur,Jin Sang Wang,Jeong Won Jang,Chang Wook Kim,Si Hyun Bae,Sung Key Jang,Se-Hwan Yang,Young Chul Sung,Oh-Joo Kwon,Seung Kew Yoon 생화학분자생물학회 2005 Experimental and molecular medicine Vol.37 No.2
Liver cirhosis is one of the major complications of hepatitis C virus (HCV) infection, but the me-chanisms underlying HCV-related fibrogenesis are stil not clear. Although the roles of HCV core protein remain poorly understood, it is supposed to play an important role in the regulation of celular growth and hepatocarcinogenesis. The aim of this study was to examine the role of HCV established an in vitro co-culture system with primary hepatic stellate cel (HSC) isolated from rats, and a stable HepG2-HCV core cell line which had been transfected with HCV core gene. The expressions of fibrosis-related molecules trans-forming growth factor β1 (TGF-β1), transforming growth factor β receptor II (TGFβRII), α-smoth muscle actin (α-SMA) and conective tisue growth factor (CTGF) were analyzed via histo-pression levels of matrix metaloprotinase-2 (MP- 2) and colagen type I (Col I) from the co-cultured media were measured by zymogram and ELISA, respectively. The expresions of α-SMA, TGF-β1, Col I, TGFβRII and MP-2 were significantly in-creased in the co-culture of stable HepG2-HCV core with HSC. Moreover, the significant increases of CTGF and TGF-β1 in the HCV core- expresing cels were observed by either Northern or Wes-tern blot analysis. These results sugest that HCV core protein may contribute to the hepatic fibro-via up-regulation of CTGF and TGF-β1.
어깨충돌증후군에 대한 침치료의 효과: 체계적 문헌고찰 및 메타분석
안정훈 ( Jeong Hoon Ahn ),배건희 ( Gun Hee Bae ),김병준 ( Byung-jun Kim ),박인화 ( In-hwa Park ),허인 ( In Heo ),차윤엽 ( Yun-yeop Cha ) 한방재활의학과학회 2024 한방재활의학과학회지 Vol.34 No.1
Objectives This review was conducted to evaluate the therapeutic effects of manual acupuncture (MA) for shoulder impingement syndrome (SIS). Methods We searched 12 electronic databases (DBpia, Korean studies Information Service System [KISS], Oriental Medicine Advanced Searching Integrated System [OASIS], Research Information Sharing Service [RISS], China National Knowledge Infrastructure [CNKI], CINAHL, Clinical Key, Cochrane Library, Embase, JAMA, PubMed, Web of Science) to find randomized-controlled clinical trials (RCTs) investigating therapeutic effects of MA for treating SIS. Shoulder Pain and Disability Index scores and numeric pain rating scale or visual analogue scale were analyzed as the main evaluation criteria. Results Among 181 studies, 169 were screened and only 12 RCTs were eligible in our review. Finally, 11 RCTs could be statistically analyzed. MA was more effective than sham treatment and physical therapy in terms of reducing pain (p=0.003, p=0.0007 each). Electroacupuncture (EA) showed more significant effect than physical therapy (PT) for improving shoulder pain (p<0.00001) and shoulder functionality (p<0.00001). Conclusions These results suggest that MA and EA could be superior option for treating SIS than sham treatment or PT. However this review has its limitations due to the small sample size and lack of well-designed RCTs that were included in the study. Further well-designed RCTs are necessary to provide high-level evidence. (J Korean Med Rehabil 2024;34(1):83-95)
호상 요구르트에 적합한 Lactobacillus plantarum strains 탐색 및 요구르트의 이화학적 및 관능 특성
정성엽(Seong-Yeop Jeong),이용현(Yong Hyen Lee),강선아(Suna Kang),신배근(Bae Keun Shin),박선민(Sunmin Park) 한국식품과학회 2016 한국식품과학회지 Vol.48 No.6
본 연구에서는 커드가 잘 생성 되는 김치 젖산세균을 탐색 및 동정을 통한 L. plantarum YD2, YD9, YD12을 분리하였으며, 분리한 젖산세균을 이용한 요구르트를 제조하여 이화학적인 특성을 알아보았다. pH와 산도의 변화는 발효시간에 따라 크게 달라졌으며, 12시간 이후 pH는 감소하는 반면 산도는 증가하였다. 점성의 경우 젖산세균의 pH와 산도에 따라 값의 변화가 있었고, pH가 낮고 산도가 높은 경우, 시간에 지남에 따라 점성 값이 감소하는 경향을 보았다. 이는 생성된 유기산이 단백질의 변성을 일으켜 요구르트 내 겔의 형성을 방해하여 점성을 떨어뜨리는 것으로 사료된다. 요구르트의 이화학적 실험 결과에 있어서 김치에서 얻어낸 젖산세균인 L. plantarum에서 커드를 형성하는 균주를 이용하여 요구르트를 제조하였을 때, 커드의 형성이 잘 이루어졌으므로, 요구르트 제조에 있어서 L. plantarum 젖산세균이 적합한 것으로 사료된다. 관능검사 결과, 대조군을 제외한 실험군 중 YD12군이 전체적인 기호도에서 가장 높은 값을 얻었으나 대조군에 비하여 낮은 값을 나타내었다. YD12군을 이용해 요구르트를 제조할 때 풍미와 신맛, 쓴맛을 보완시킨다면 시판되는 요구르트와 유사한 혹은 더 높은 기호도를 얻을 수 있을 것으로 기대한다. 김치 젖산세균을 이용한 요구르트 제조 시, 김치 젖산세균이 위산에 대해 내성을 가지고 있으므로 젖산세균이 장까지 잘 전달이 될 것 이라 기대한다. Since some strains of kimchi lactobacilli can curdle milk, they can be used for making yogurt. However, the best Lactobacillus plantarum strains for curdling milk for yogurt are still unknown. In this study, we determined the best L. plantarum strains for curdling milk, and the physicochemical properties of yogurts made using different L. plantarum strains were examined. Three strains of L. plantarum useful for curdling milk were identified (YD2, YD9, YD12). The number of lactobacilli was lower in yogurts made with L. plantarum than in those made with control, and among the L. plantarum strains tested, YD12 had the highest bacterial counts. However, the microbial count reached 6.3×10<SUP>8</SUP> CFU/mL after 24-h fermentation in all yogurts. The pH of the yogurts decreased after 12-h fermentation, while the acidity increased. The low pH and high acidity decreased the viscosity in all the three types of yogurts, because the acids disturbed gel formation due to protein denaturation. Sensory evaluation revealed that the YD12 group showed a high percentage of completion similar to the control group. YD2 and YD9 showed a high sourness value and low sweetness alue, whereas YD12 yielded optimal values for all the organoleptic characteristics. Therefore, YD12 would be a high quality bacterial strain for use as a yogurt starter culture.
Health Impact Assessment of Free Immunization Program in Jinju City, Korea
Kim, Keon-Yeop,Jeon, So-Youn,Jeon, Man-Joong,Lee, Kwon-Ho,Lee, Sok-Goo,Kim, Dong-Jin,Kang, Eun-Jeong,Bae, Sang-Geun,Kim, Jin-Hee The Korean Society for Preventive Medicine 2012 예방의학회지 Vol.45 No.4
Objectives: This study was conducted to assess the potential health impacts and improve the quality of the free immunization program in Jinju City by maximizing the predicted positive health gains and minimizing the negative health risks. Methods: A steering committee was established in September 2010 to carry out the health impact assessment (HIA) and began the screening and scoping stages. In the appraisal stage, analysis of secondary data, a literature review, case studies, geographic information systems analysis, a questionnaire, and expert consultations were used. The results of the data collection and analyses were discussed during a workshop, after which recommendations were finalized in a written report. Results: Increased access to immunization, comprehensive services provided by physicians, the strengthened role of the public health center in increasing immunization rates and services, and the ripple effect to other neighboring communities were identified as potential positive impacts. On the other hand, the program might be inaccessible to rural regions with no private clinics where there are more at-risk children, vaccine management and quality control at the clinics may be poor, and vaccines may be misused. Recommendations to maximize health gains and minimize risks were separately developed for the public health center and private clinics. Conclusions: The HIA provided an opportunity for stakeholders to comprehensively overview the potential positive and negative impacts of the program before it was implemented. An HIA is a powerful tool that should be used when developing and implementing diverse health-related policies and programs in the community.
당뇨병이 없는 성인의 비만 여부에 따른 공복혈당장애 관련 요인
이수정(Su-Jeong Lee),김건엽(Keon-Yeop Kim),김명관(Myung-Gwan Kim),남행미(Hang-Me Nam),배상근(Sang-Geun Bae) 한국산학기술학회 2017 한국산학기술학회논문지 Vol.18 No.6
본 연구는 정상 성인을 대상으로 공복혈당 장애 정도와 공복혈당 장애율과 관련된 요인을 파악하여 향후 당뇨병으로 진행하는 것을 예방하는데 도움을 주고자 실시 하였다. 연구 대상은 2014년 국민건강영양조사 자료 대상자인 7,550명 중 당뇨병이 있거나 무응답자를 제외한 20∼59세의 당뇨병이 없는 정상 성인 1,341명으로 하였다. 연구 방법은 대상자를 비만군과 정상 체중군으로 나누고, 인구학적 특성(성, 연령, 가구 소득, 교육 수준, 직업 등), 건강행태 특성(음주, 흡연, 걷기, 과일섭취 빈도 등), 비만도 및 공복혈당 장애 정도를 조사하였다. 연구결과 비만군 및 정상 체중군 모두 연령이 높은 경우 공복혈당 장애율이 유의하게 높았다 (p<0.001). 비만군에서는 과일섭취 빈도가 적은 경우와 음주를 많이 하는 경우 공복혈당 장애율이 유의하게 높았다 (p<0.05). 따라서 당뇨병이 없는 성인들의 경우 당뇨병으로 진행하는 것을 예방하기 위해 비만군과 정상체중군에 따른 접근 전략을 달리해야 하며, 특히 비만 성인을 대상으로 적절한 과일 섭취와 절주를 위한 교육 프로그램과 보건의료 서비스 정책이 필요하다. This study examined the prevalence of an impaired fasting glucose and related factors in normal non-diabetic adults. This study used the raw data (general characteristics, health behavior, obesity, impaired fasting glucose etc.) from the 6th Korea National Health & Nutrition Examination Survey (NHANES) in the second year (2014), and out of all 7,550 survey participants, 1,341 were selected as the final research subjects. After stratifying according to obesity, a logistic regression model was used to analyze the factors related to an impaired fasting glucose level. The older subjects were more likely to have an impaired fasting glucose in both the obesity group and normal weight group (p<.001). The obesity group showed a higher impaired fasting glucose rate when they drank more alcohol (p<.05) and consumed less fruit (p<.05). Therefore, balanced nutrients and control of the blood sugar level through proper education and public health policies offruit intake and alcohol are needed.