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Augustine Omoniyi Ayeni,Ram Awtar Pandey,James Abiodun Omoleye,Sandeep Mudliar,Fredrick Kofi Hymore 한국화학공학회 2014 Korean Journal of Chemical Engineering Vol.31 No.7
Enzymatic hydrolysis and fermentation methods were evaluated on alkaline peroxide pretreated shea treesawdust conversion to ethanol. Optimum pretreatment conditions of 120 oC reaction temperature, 30min reaction time,and 20 mL L−1 of water hydrogen peroxide concentration (1%(v/v)H2O2) solubilized 679 g kg−1 of hemicellulose and172 g kg−1 of lignin. 617 g kg−1 cellulose was retained in the solid fraction. The maximum yield of reducing sugar withoptimized enzyme loadings by two enzyme preparations (cellulase and β-glucosidase) was 165 g kg−1 of dry biomass. The ethanol yield was 7.35 g L−1 after 72 h incubation period under the following conditions: 2% cellulose loading,enzyme concentration was 25 FPU (g cellulose)−1 loading, yeast inoculums was 10% (v/v), 32 oC, and pH 4.8. Thepretreatments gave information about the hindrances caused by lignin presence in lignocellulosic materials and thathemicelluloses are better hydrolyzed than lignin, thereby enhancing enzymatic digestibility of the sawdust material.