Starch composition, glycemic indices, phenolic constituents, and antioxidative and antidiabetic properties of some common tropical fruits

Ganiyu Oboh,Ayokunle O. Ademosun,Margareth Akinleye,Olasunkanmi S. Omojokun,Aline A. Boligon,Margareth L. Athayde 한국식품연구원 2015 Journal of Ethnic Foods Vol.2 No.2

Background: Studies have supported the protective effect of high fruit consumption in the management of chronic diseases such as diabetes. Methods: Thirteen fresh tropical fruits were sourced for and the fruits juices were extracted, freeze dried, and then reconstituted for analysis. The sugar, starch, amylose, and amylopectin contents as well as glycemic indices, antioxidant properties, and the ability of the fruits to inhibit starch-hydrolyzing enzymes were determined. Also, the phenolic constituents of the fruits were characterized using highperformance liquid chromatography coupled with diode array detector. Results: The starch, sugar, amylase, and amylopectin contents were 3.01-3.89 g/100 g, 35.34-60.91 g/ 100 g, 0.84-1.46 g/100 g, and 1.68-2.86 g/100 g, respectively, while the glycemic indices were 28.01 e68.34, with African star apple (28.01) having the lowest and watermelon (68.34) the highest. Furthermore, the fruits exhibited high antioxidant properties as exemplified by their DPPH, ABTS+, OH, and NO radical scavenging abilities. Likewise, the fruits also demonstrated a-amylase and a-glucosidase inhibitory property with Soursop (IC50 = 18.52 mg/mL), guava (IC50 = 19.77 mg/mL), and African star apple (IC50 = 20.86 mg/mL) showing the highest inhibitory potential among the 13 fruits. Similarly, the same trend was followed for a-glucosidase inhibitory activity. Conclusion: The fruits' low glycemic indices, strong antioxidant properties, and inhibition of a-amylase and a-glucosidase activities could be possible mechanisms for their use in the management and prevention of type-2 diabetes.

Effect of Two Ginger Varieties on Arginase Activity in Hypercholesterolemic Rats

Ayodele Jacob Akinyemi,Ganiyu Oboh,Adedayo Oluwaseun Ademiluyi,Aline Augusti Boligon,Margareth Linde Athayde 사단법인약침학회 2016 Journal of Acupuncture & Meridian Studies Vol.9 No.2

Recently, ginger has been used in traditional Chinese medicine as an herbal therapy for treating several cardiovascular diseases, however, information on its mechanism of action is limited. The present study assessed the effect of two ginger varieties (Zingiber officinale and Curcuma longa) on the arginase activity, atherogenic index, levels of liver thiobarbituric acid reactive substances (TBARSs), and plasma lipids in rats fed with a high-cholesterol (2%) diet for 14 days. Following the treatment period, it was found that feeding a high-cholesterol diet to rats caused significant (p < 0.05) increases in arginase activity, atherogenic index, levels of TBARS, total cholesterol (TC), triglycerides (TGs), and low-density lipoprotein cholesterol (LDL-C) with a concomitant decrease in highdensity lipoprotein cholesterol (HDL-C). However, both ginger and turmeric (2% and 4%) caused significant (p < 0.05) decreases in arginase activity and the atherogenic index, and prevented hypercholesterolemia by decreasing the TC, TGs, and LDL-C while increasing the HDL-C when compared with the controls. In conclusion, dietary supplementation with both types of rhizomes (ginger and turmeric) inhibited arginase activity and prevented hypercholesterolemia in rats that received a high-cholesterol diet. Therefore, these activities of ginger and turmeric represent possible mechanisms underlying its use in herbal medicine to treat several cardiovascular diseases.

In Vitro Antioxidant Activity and Effect of Parkia biglobosa Bark Extract on Mitochondrial Redox Status

Kayode Komolafe,Tolulope Mary Olaleye,Olaposi Idowu Omotuyi,Aline Augusti Boligon,Margareth Linde Athayde,Akintunde Afolabi Akindahunsi,Joao Batista Teixeira da Rocha 사단법인약침학회 2014 Journal of Acupuncture & Meridian Studies Vol.7 No.4

Aqueous-methanolic extract of Parkia biglobosa bark (PBB) was screened for its polyphe- nolic constituents, in vitro antioxidant activity, and effect on mitochondria redox status. The in vitro antioxidant activity was assessed by using the scavenging abilities and the reducing powers of 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) and 2,2 0 -azino-bis(3- ethylbenzothiazoline-6-sulfonic acid) (ABTS) diammonium salt radical cation against Fe 3þ . Subsequently, the ability of PBB to inhibit lipid peroxidation induced by FeSO 4 (10 mm) and its metal-chelating potential were investigated. The effects of the extract on basal reactive oxygen species (ROS) generation and on the mitochondrial membrane potential (DJm) in isolated mitochondria were determined by using 2 0 , 7 0 -dichlorodihy- drofluorescin (DCFH) oxidation and safranin fluorescence, respectively. PBB mitigated the Fe(II)-induced lipid peroxidation in rat tissues and showed dose-dependent scav- enging of DPPH (IC 50 : 98.33 ± 10.0 mg/mL) and ABTS. (trolox equivalent antioxidant concentration, TEAC value = 0.05), with considerable ferric-reducing and moderate metal-chelating abilities. PBB caused slight decreases in both the liver and the brain mitochondria potentials and resulted in a significant decrease (p < 0.001) in DCFH oxida- tion. Screening for polyphenolics using high-performance liquid chromatography coupled to a diode array detector (HPLC-DAD) revealed the presence of caffeic acid, gallic acid, catechin, epigalocatechin, rutin, and quercetin. These results demonstrate for the first time the considerable in vitro antioxidant activity and favorable effect of PBB on mito- chondria redox status and provide justification for the use of the plant in ethnomedicine.

Antioxidant Properties of Taraxacum officinale Leaf Extract Are Involved in the Protective Effect Against Hepatoxicity Induced by Acetaminophen in Mice

Dirleise Colle,Leticia Priscilla Arantes,Priscila Gubert,Soˆnia Cristina Almeida da Luz,Margareth Linde Athayde,Joa˜o Batista Teixeira ocha,Fe´lix Alexandre Antunes Soares 한국식품영양과학회 2012 Journal of medicinal food Vol.15 No.6

Acetaminophen (APAP) hepatotoxicity has been related to several cases of hepatitis, cirrhosis, and hepatic transplant. As APAP hepatotoxicity is related to reactive oxygen species (ROS) formation and excessive oxidative stress,natural antioxidant compounds have been tested as an alternative therapy to diminish the hepatic dysfunction induced by APAP. Taraxacum officinale Weber (Family Asteraceae), commonly known as dandelion, is used for medicinal purposes because of its choleretic, diuretic, antioxidant, anti-inflammatory, and hepatoprotective properties. This study evaluated the hepatoprotective activity of T. officinale leaf extract against APAP-induced hepatotoxicity. T. officinale was able to decrease thiobarbituric acid–reactive substance levels induced by 200 mg/kg APAP (p.o.), as well as prevent the decrease in sulfhydryl levels caused by APAP treatment. Furthermore, histopathological alterations, as well as the increased levels of serum aspartate and alanine aminotransferases caused by APAP, were prevented by T. officinale (0.1 and 0.5 mg/mL). In addition, T. officinale extract also demonstrated antioxidant activity in vitro, as well as scavenger activity against 2,2-diphenyl-1-picrylhydrazyl and nitric oxide radicals. Our results clearly demonstrate the hepatoprotective effect of T. officinale against the toxicity induced by APAP. The possible mechanisms involved include its scavenger activities against ROS and reactive nitrogen species, which are attributed to the content of phenolic compounds in the extract.

Sorghum [Sorghum bicolor (L.) Moench] Leaf Sheath Dye Protects Against Cisplatin-Induced Hepatotoxicity and Oxidative Stress in Rats

Adedayo O. Ademiluyi,Ganiyu Oboh,Oluwaseun J. Agbebi,Aline A. Boligon,Margareth L. Athayde 한국식품영양과학회 2014 Journal of medicinal food Vol.17 No.12

This study sought to determine the protective effect of dietary inclusion of sorghum leaf sheath dye on cisplatin-induced hepatotoxicity and oxidative stress in rats. Adult male rats were randomly divided into four groups with six animals in each group. Groups I and II were fed a basal diet, while groups III and IV were fed diets containing 0.5% and 1% sorghum leaf sheath dye, respectively, for 20 days before cisplatin administration. Hepatotoxicity was induced by a single dose of cisplatin (7 mg/kg body weight, i.p.), and the experiment was terminated at 3 days after cisplatin injection. The liver and plasma were studied for hepatotoxicity and antioxidant capacity. Cisplatin caused a significant (P < .05) alteration in plasma and liver enzymatic (catalase, glutathione-S-transferase [GST], and superoxide dismutase [SOD]) and nonenzymatic (glutathione [GSH] and vitamin C) antioxidant indices with a concomitant increase in the malondialdehyde (MDA) content; however, there was a significant (P < .05) restoration of the antioxidant status coupled with a significant (P < .05) decrease in the tissue MDA content, after consumption of diets containing sorghum leaf sheath dye. Furthermore, dietary inclusion of sorghum leaf sheath dye caused a marked reduction in the activities of alanine aminotransferase and aspartate aminotransferase after cisplatin administration. However, the ability of the dye to prevent significant cisplatin-induced alteration of both plasma and liver antioxidant indices suggests an antioxidant mechanism of action. Hence, this protective effect of Sorghum bicolor leaf sheath dye against cisplatin-induced hepatotoxicity in rats reflects its potential and beneficial role in the prevention of liver damage associated with cisplatin administration.

In Vitro Trypanocidal Activity of Macela (Achyrocline satureioides) Extracts against Trypanosoma evansi

Matheus D. Baldissera,Camila B. Oliveira,Carine E. P. Zimmermann,Aline A. Boligon,Margareth Linde Athayde,Leandro P. Bolzan,Rodrigo de A. Vaucher,Janio M. Santurio,Michele R. Sagrillo,Aleksandro Schaf 대한기생충학열대의학회 2014 The Korean Journal of Parasitology Vol.52 No.3