N-acetyl-L-cysteine and cysteine increase intracellular calcium concentration in human neutrophils

Md. Ashraful Hasan,안원균,송동근 대한약리학회 2016 The Korean Journal of Physiology & Pharmacology Vol.20 No.5

N-acetyl-L-cysteine (NAC) and cysteine have been implicated in a number of human neutrophils’ functional responses. However, though Ca2+ signaling is one of the key signalings contributing to the functional responses of human neutrophils, effects of NAC and cysteine on intracellular calcium concentration ([Ca2+]і) in human neutrophils have not been investigated yet. Thus, this study was carried out with an objective to investigate the effects of NAC and cysteine on [Ca2+]і in human neutrophils. We observed that NAC (1 mM ~ 1 mM) and cysteine (10 mM ~ 1 mM) increased [Ca2+]і in human neutrophils in a concentration-dependent manner. In NAC pre-supplmented buffer, an additive effect on N-formyl-methionine-leucinephenylalanine (fMLP)-induced increase in [Ca2+]i in human neutrophils was observed. In Ca2+-free buffer, NAC- and cysteine-induced [Ca2+]i increase in human neutrophils completely disappeared, suggesting that NAC- and cysteine-mediated increase in [Ca2+]i in human neutrophils occur through Ca2+ influx. NAC- and cysteine-induced [Ca2+]і increase was effectively inhibited by calcium channel inhibitors SKF96365 (10 mM) and ruthenium red (20 mM). In Na+-free HEPES, both NAC and cysteine induced a marked increase in [Ca2+]i in human neutrophils, arguing against the possibility that Na+-dependent intracellular uptake of NAC and cysteine is necessary for their [Ca2+]і increasing activity. Our results show that NAC and cysteine induce [Ca2+]і increase through Ca2+ influx in human neutrophils via SKF96365- and ruthenium reddependent way.

Anti-inflammatory and anti-noceceptive action of the crude extracts of Costus specious on rodents

Alam, Ashraful,Subhan, Nusrat,Awal, Abdul,Alam, Shohidul,Akramudau, Kazi Kyung Hee Oriental Medicine Research Center 2008 Oriental pharmacy and experimental medicine Vol.8 No.3

The effect of alcoholic extracts of Costus specious (Family: Zingiberaceae) was evaluated in experimental models of pain and inflammation. Oral administration of 100, 200 and 300 mg/kg of C. specious extracts were used for the above study. Crude extracts of C. specious (300 mg/kg dose) showed maximum time needed for the response against thermal stimuli ($7.242\;{\pm}\;0.532\;s$) which is comparable to diclofenac sodium ($8.471\;{\pm}\;0.25\;s$) in the hot plate test. The MPH (Maximum Possible Analgesia) has been found to be 14.285 for 300 mg/kg dose of the crude extract while the MPH for diclofenac was 15.857 after 60 min of administration in the hot tail-flick method. The crude extract at 300 and 200 mg/kg doses showed significant reduction in acetic acid induced writhings in mice with a maximum effect of 59.661% reduction at 300 mg/kg dose which is comparable to standard diclofenac sodium (73.4%). Alcoholic extract of C. specious showed significant inhibition in serotonin and egg albumin induced hind paw oedema in rats at 100, 200 and 300 mg/kg of the crude extracts respectively (Serotonin induced edema 44.22; 53.75; 58.51%; egg albumin induced edema - 41.317; 53.892; 59.880% inhibition after 4 h respectively). The antiinflammatory effects showed by the extract were comparable to that of standard indomethacin 5 mg/kg (Serotonin induced edema 77.56%; egg albumin induced edema 77.844% inhibition after 4 h). These results suggest that the extract possesses both the anti-inflammatory and analgesic activity on mice and rat model.

Two-Dimensional WO₃ Nanosheets Chemically Converted from Layered WS₂ for High-Performance Electrochromic Devices

Azam, Ashraful,Kim, Jungmo,Park, Junyong,Novak, Travis G.,Tiwari, Anand P.,Song, Sung Ho,Kim, Bumsoo,Jeon, Seokwoo American Chemical Society 2018 NANO LETTERS Vol.18 No.9

<P>Two-dimensional (2D) transitional metal oxides (TMOs) are an attractive class of materials due to the combined advantages of high active surface area, enhanced electrochemical properties, and stability. Among the 2D TMOs, 2D tungsten oxide (WO<SUB>3</SUB>) nanosheets possess great potential in electrochemical applications, particularly in electrochromic (EC) devices. However, feasible production of 2D WO<SUB>3</SUB> nanosheets is challenging due to the innate 3D crystallographic structure of WO<SUB>3</SUB>. Here we report a novel solution-phase synthesis of 2D WO<SUB>3</SUB> nanosheets through simple oxidation from 2D tungsten disulfide (WS<SUB>2</SUB>) nanosheets exfoliated from bulk WS<SUB>2</SUB> powder. The complete conversion from WS<SUB>2</SUB> into WO<SUB>3</SUB> was confirmed through crystallographic and elemental analyses, followed by validation of the 2D WO<SUB>3</SUB> nanosheets applied in the EC device. The EC device showed color modulation of 62.57% at 700 nm wavelength, which is 3.43 times higher than the value of the conventional device using bulk WO<SUB>3</SUB> powder, while also showing enhancement of ∼46.62% and ∼62.71% in switching response-time (coloration and bleaching). The mechanism of enhancement was rationalized through comparative analysis based on the thickness of the WO<SUB>3</SUB> components. In the future, 2D WO<SUB>3</SUB> nanosheets could also be used for other promising applications such as sensors, catalysis, thermoelectric, and energy conversion.</P> [FIG OMISSION]</BR>

Sequential Multi-Directional Elemental Image Projection Scheme for the Viewing Angle Enhancement of Integral Imaging 3D Display

Md. Ashraful Alam,Kaniz Fatema Kakolee,Nam Kim 한국콘텐츠학회 2014 한국콘텐츠학회 ICCC 논문집 Vol.- No.-

Safety of the oral cholera vaccine in pregnancy: Retrospective findings from a subgroup following mass vaccination campaign in Dhaka, Bangladesh ^☆

Khan, Ashraful Islam,Ali, Mohammad,Chowdhury, Fahima,Saha, Amit,Khan, Iqbal Ansary,Khan, Arifuzzaman,Akter, Afroza,Asaduzzaman, Muhammad,Islam, Md. Taufiqul,Kabir, Alamgir,You, Young Ae,Saha, Nirod Ch Elsevier Science 2017 Vaccine Vol.35 No.11

<▼1><P><B>Highlights</B></P><P>•<P>Few women received the OCV unknowingly while pregnant during a large vaccine trial.</P>•<P>There is limited data on the safety of OCVs in pregnancy.</P>•<P>We evaluated the effect of a killed OCV, Shanchol™, on pregnancy outcomes.</P>•<P>Study showed no evidence of exposure to Shanchol™ on adverse pregnancy outcomes.</P></P></▼1><▼2><P><B>Background</B></P><P>Pregnant women are vulnerable to complications of cholera. Killed oral cholera vaccines (OCV) are not recommended for pregnant women though there is no evidence of harmful effects during pregnancy. We evaluated the effect of a killed OCV, Shanchol™, on pregnancy outcomes during an effectiveness trial of the vaccine in urban Bangladesh.</P><P><B>Methodology</B></P><P>Individuals ⩾1 year were invited to participate in the trial, conducted in 2011 in Dhaka, Bangladesh. Pregnancy by history was an exclusion criterion and all women of reproductive age (15–49 years) were verbally questioned about pregnancy at enrollment and prior to vaccination. Out of 48,414 women of reproductive age 286 women received the OCV unknowingly while pregnant. Out of these, we could recruit 69 women defined as exposed to OCV. Accordingly, we selected 69 pregnant women randomly from those who did not take the OCV (non-exposed to OCV). We evaluated adverse pregnancy outcome (spontaneous miscarriages, still births, or congenital malformations) between those who were exposed to OCV and those who were not exposed to OCV.</P><P><B>Results</B></P><P>About 16% of pregnant women exposed to OCV had pregnancy loss, as compared to 12% of unvaccinated pregnant women (P = 0.38). One congenital anomaly was observed and occurred in women non-exposed to OCV group. Models that adjusted for baseline characteristics that were unbalanced between the exposed and non-exposed groups, revealed a no elevation of risk of adverse pregnancy outcomes in vaccinees versus non-vaccinees (Adj. OR (95% CI): 0.45 (0.11–1.88).</P><P><B>Conclusions</B></P><P>No excess of adverse fetal outcomes associated with receipt of OCV was observed in this study.</P><P>Trial registration: Clinical Trials.gov number NCT01339845.</P></▼2>

Evaluation of carbon dioxide emission factor from urea applied in upland soil during garlic cultivation

Muhammad Ashraful Alam,Pil Joo Kim 한국토양비료학회 2016 한국토양비료학회 학술발표회 초록집 Vol.2016 No.10

Reevaluation of N₂O emission factor from temperate upland soil

Muhammad Ashraful Alam,Pil Joo Kim 한국토양비료학회 2017 한국토양비료학회 학술발표회 초록집 Vol.2017 No.10

COUNTRY RISK ASSESSMENT USING DISCRIMINANT ANALYSIS

Mohammed Ashraful Haque People&Global Business Association 1996 Global Business and Finance Review Vol.1 No.2

The purpose of this study is to identify the economic and financial variables which affect a country's credit worthiness. Once the variables are identified, a model is developed using discriminant analysis to separate the forty developing countries used in this study into three specific risk categories: (1) low risk, (2) medium risk, and (3) high risk. Several financial and economic variables are tested, but only six were considered significant enough to be used in the model. These six variables are gross national product per capita, debt service ratio, export, import, debt outstanding and disbursed as a percentage of GNP and, finally, international reserve to debt outstanding.

Season Dependent Variation in Nitrous Oxide Emission Factor of Urea in Temperate Upland Soil

Muhammad Ashraful Alam(무하마드 아쉬라풀 알람),Kim Pil Joo(김필주) 한국토양비료학회 2018 한국토양비료학회 학술발표회 초록집 Vol.2018 No.10

Anti-inflammatory and anti-noceceptive action of the crude extracts of Costus specious on rodents

Md. Ashraful Alam,Nusrat Subhan,Md. Abdul Awal,Md. Shohidul Alam,Kazi Akramudaulla 경희대학교 융합한의과학연구소 2008 Oriental Pharmacy and Experimental Medicine Vol.8 No.3

The effect of alcoholic extracts of Costus specious (Family: Zingiberaceae) was evaluated in experimental models of pain and inflammation. Oral administration of 100, 200 and 300 mg/kg of C. specious extracts were used for the above study. Crude extracts of C. specious (300 mg/kg dose) showed maximum time needed for the response against thermal stimuli (7.242 ± 0.532 s) which is comparable to diclofenac sodium (8.471 ± 0.257 s) in the hot plate test. The MPH (Maximum Possible Analgesia) has been found to be 14.285 for 300 mg/kg dose of the crude extract while the MPH for diclofenac was 15.857 after 60 min of administration in the hot tail-flick method. The crude extract at 300 and 200 mg/kg doses showed significant reduction in acetic acid induced writhings in mice with a maximum effect of 59.661% reduction at 300 mg/kg dose which is comparable to standard diclofenac sodium (73.4%). Alcoholic extract of C. specious showed significant inhibition in serotonin and egg albumin induced hind paw oedema in rats at 100, 200 and 300 mg/kg of the crude extracts respectively (Serotonin induced edema 44.22; 53.75; 58.51%; egg albumin induced edema - 41.317; 53.892; 59.880% inhibition after 4 h respectively). The antiinflammatory effects showed by the extract were comparable to that of standard indomethacin 5 mg/kg (Serotonin induced edema 77.56%; egg albumin induced edema 77.844% inhibition after 4 h). These results suggest that the extract possesses both the anti-inflammatory and analgesic activity on mice and rat model. The effect of alcoholic extracts of Costus specious (Family: Zingiberaceae) was evaluated in experimental models of pain and inflammation. Oral administration of 100, 200 and 300 mg/kg of C. specious extracts were used for the above study. Crude extracts of C. specious (300 mg/kg dose) showed maximum time needed for the response against thermal stimuli (7.242 ± 0.532 s) which is comparable to diclofenac sodium (8.471 ± 0.257 s) in the hot plate test. The MPH (Maximum Possible Analgesia) has been found to be 14.285 for 300 mg/kg dose of the crude extract while the MPH for diclofenac was 15.857 after 60 min of administration in the hot tail-flick method. The crude extract at 300 and 200 mg/kg doses showed significant reduction in acetic acid induced writhings in mice with a maximum effect of 59.661% reduction at 300 mg/kg dose which is comparable to standard diclofenac sodium (73.4%). Alcoholic extract of C. specious showed significant inhibition in serotonin and egg albumin induced hind paw oedema in rats at 100, 200 and 300 mg/kg of the crude extracts respectively (Serotonin induced edema 44.22; 53.75; 58.51%; egg albumin induced edema - 41.317; 53.892; 59.880% inhibition after 4 h respectively). The antiinflammatory effects showed by the extract were comparable to that of standard indomethacin 5 mg/kg (Serotonin induced edema 77.56%; egg albumin induced edema 77.844% inhibition after 4 h). These results suggest that the extract possesses both the anti-inflammatory and analgesic activity on mice and rat model.