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- 저자 : Asan-Ozusaglam (검색결과 3 건)
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Meltem Asan-Ozusaglam,Ayse Gunyakti 한국식품과학회 2019 Food Science and Biotechnology Vol.28 No.2
The study aimed to identify two lactic acidbacteria from human breast milk and evaluate for theirprobiotic potential. Lactobacillus fermentum strainsshowed various degree of antibiotic susceptibility profile toclinical commercial antibiotics. The MA-7 and MA-8strains have never lost their vitality at pH 2 and 3. Lactobacillusfermentum MA-8 exhibited more resistance to 0.3and 1% bile concentrations than MA-7 strain. Both of theL. fermentum strains can tolerate the simulated gastric andintestinal juices. The strains showed high auto-aggregationpercentages varied from 95 to 98%. Both isolates alsoexhibited gamma hemolytic activity. The cholesterol loweringactivity of MA-7 and MA-8 strains was varied from34.84 to 91.15%. The supernatants showed higher anticholesterolactivity compared to pellets. The resultsdemonstrated that both L. fermentum strains maybe goodprobiotic candidates and may have potential as healthbiotherapeutic with cholesterol-lowering effects.
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Murat Kaya,Talat Baran,Meltem Asan-Ozusaglam,Yavuz Selim Cakmak,Kabil Ozcan Tozak,Abbas Mol,Ayfer Mentes,Goksal Sezen 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.1
This study examined two gregarious Orthopteraspecies (Calliptamus barbarus and Oedaleus decorus) aspotential sources of chitin. The chitin content of the dryweight of C. barbarus was 20.5 ± 0.7%, and it was 16.5 ±0.7% for O. decorus. Furthermore, the yield of chitosan (70~ 75% deacetylation degree) from the grasshopper specieswas found to be 74 ~ 76%, which is close to the yield ofcommercial preparations obtained from the unused parts ofcrabs and shrimp. The chitin and chitosan obtained in thisway were analyzed using FTIR, TGA, XRD and SEMtechniques, and the antimicrobial properties of chitosansobtained from C. barbarus and O. decorus against pathogenicmicroorganisms of humans and fish were investigatedusing the disc diffusion and microdilution broth methods. The antimicrobial screening procedures indicated that thechitosan showed significant antimicrobial activity againstall of the tested pathogenic microorganisms. The MBC orMFC values were determined to be 0.16 ~ 2.50 mg/mL. The IC50 values for the chitins obtained from C. barbarusand O. decorus were 10.68 ± 0.27 and 10.91 ± 0.96 mg/mL,respectively, which were greater than the value forbutylated hydroxytoluene (BHT): 0.04 ± 0.01 mg/mL. These results suggest that these two species, which arecurrently considered to be pests because of over-breeding,are potentially alternative sources of chitin and chitosan,which are used in the food/feed industry for theirantimicrobial and antioxidant properties.
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Murat Kaya,Yavuz Selim Cakmak,Talat Baran,Meltem Asan-Ozusaglam,Ayfer Mentes,Kabil Ozcan Tozak 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.1
The paper describes the isolation and characterizationof chitin and chitosan from Daphnia longispinaresting eggs harvested from a reservoir. Resting eggs arefertilized eggs that are encased in chitinous shells called‘ephippia’ and which ensure the survival of the Daphniapopulation in adverse conditions. The chitin-content ofD. longispina resting eggs was found to be 23 ~ 25% andthe chitosan (having a 70 ~ 75% deacetylation degree)yield of the chitin was 76 ~ 77%. This high chitin-contentindicates that D. longispina resting eggs can be exploitedas a chitin source. The structure and thermal properties ofchitin, extracted from D. longispina resting eggs, werecharacterized by employing Fourier transform infraredspectroscopy, thermogravimetric analysis, X-ray diffractionand scanning electron microscopy. The crystallinity of thechitin was found to be very low (48%). Physicochemicallycharacterizedchitosan and the produced O-carboxymethylchitosan were tested for their antimicrobial and antioxidantactivity. It has been observed that chitosan displaysantimicrobial activity against all pathogenic bacteria, whereasO-carboxymethyl chitosan only exhibits inhibition activityagainst L. garvieae, L. Monocytogenes ATCC 7644, Y. enterocolitica NCTC 11175 and S. aureus ATCC 25923. Ina free radical scavenging activity assay, the IC50 values ofchitosan, O-carboxymethyl chitosan and butylated hydroxytoluenewere found to be 23.01, 56.43 and 0.05, respectively. The ferric-reducing power of O-carboxymethyl chitosan(EC50 = 8.30) indicated higher activity than chitosan (EC50= 10.12).
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