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RISS 인기검색어
- 검색키워드
- 저자 : Arpita Ghosh (검색결과 5 건)
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Arpita Ghosh,Manisha Ghosh Dastidar,T. R. Sreekrishnan,Pradipta Patra 한국대기환경학회 2019 Asian Journal of Atmospheric Environment (AJAE) Vol.13 No.4
The efficiency of Aspergillus tamarii (isolated from sludge of a textile industry) was investigated to remediate the synthetic solutions of reactive red 120 (RR120) dye and chromium(III) separately. Also, parallel studies were conducted on the bioremediation of binary system of chromium(III) and RR120 dye in different ratios. The study was conducted to find the potential of the A. tamarii for removing chromium(III) and color from solutions containing only chromium or only RR120 dye or both and the effect of RR120 and chromium on the growth of the strain were observed. Maximum dye removal 81 mg/L was observed from 100 mg/L RR120 dye solution at pH 5 up to 50 hours. Maximum chromium removal 88.3 mg/L was observed from 100 mg/L chromium (III) solution at pH 5 up to 50 hours. In previous studies, A. tamarii was used as a bioremediator for removing different chromium complex dyes. The removal of chromium(III) and color were compared from solutions of synthetic mixtures and chromium complex dyes. The response of bioremediation study was validated using multiple regression analysis.
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Abhinav Chaudhary,Spandan Chaudhary,Arpita Ghosh,Srinivas Vuduthala,K. M. Singh,Surendra K Chikara 한국작물학회 2015 Journal of crop science and biotechnology Vol.18 No.3
A rapid and inexpensive protocol for isolation of mitochondrial DNA from Oryza sativa with negligible genomic DNA contamination is developed without use of density-gradients materials. Mitochondria were isolated from rice seedlings in an in-house lysis buffer containing sucrose followed by DNase I treatment to remove nuclear DNA. Modified CTAB method was used to isolate mitochondrial DNA from isolated mitochondria. The presence of mitochondrial DNA was confirmed by using selective amplification of mtDNA specific genes. PCR amplification was observed in all genes except β -actin gene. In addition, Sanger sequencing and gene mapping to reference gene sequences in public database was performed to confirm the presence of mitochondrial DNA. The mapping analysis showed 99.71% similarity with mitochondrial DNA. The protocol demonstrated high specificity and yielded high purity mitochondrial DNA. It is concluded that the protocol described here will be beneficial for scientific communities by providing a cheap and robust mitochondrial DNA isolation protocol for potential applications.
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Swati Dasgupta,Ujjal K Ray,Arpita Ghosh Mitra,Deboshree M. Bhattacharyya,Ashis Mukhopadhyay,Priyabrata Das,Sudeshna Gangopadhyay,Sudip Roy,Soma Mukhopadhyay 대한혈액학회 2017 Blood Research Vol.51 No.2
Background: Philadelphia chromosome, a hallmark of chronic myeloid leukemia (CML), plays a key role in disease pathogenesis. It reflects a balanced reciprocal translocation between long arms of chromosomes 9 and 22 involving BCR and ABL1 genes, respectively. An accurate and reliable detection of BCR-ABL fusion gene is necessary for the diagnosis and monitor-ing of CML. Previously, many technologies, most of which are laborious and time consum-ing, have been developed to detect BCR-ABL chimeric gene or chromosome. Methods: A new flow cytometric immunobead assay was used for detection of BCR-ABL fusion pro-teins and applicability, sensitivity, reliability, efficacy and rapidity of this method was evaluated. Results: From February 2009 to January 2014, a total 648 CML patients were investigated for the status of BCR-ABL1 protein. Among them, 83 patients were enrolled for comparative study of BCR-ABL1 positivity by three routinely used procedures like karyotyping, and quantita-tive real time PCR (RT-PCR) as well as immunobead flow cytometry assay. BCR-ABL protein analysis was found consistent, more sensitive (17% greater sensitivity) and reliable than the conventional cytogenetics, as flow cytometry showed 95% concordance rate to RT-PCR. Conclusion: BCR-ABL fusion protein assay using a new flow cytometric immunobead might be useful in the diagnosis and monitoring CML patients.
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Swati Dasgupta,Ujjal K Ray,Arpita Ghosh Mitra,Deboshree M. Bhattacharyya,Ashis Mukhopadhyay,Priyabrata Das,Sudeshna Gangopadhyay,Sudip Roy,Soma Mukhopadhyay 대한혈액학회 2017 Blood Research Vol.52 No.2
Background: Philadelphia chromosome, a hallmark of chronic myeloid leukemia (CML), plays a key role in disease pathogenesis. It reflects a balanced reciprocal translocation between long arms of chromosomes 9 and 22 involving BCR and ABL1 genes, respectively. An accurate and reliable detection of BCR-ABL fusion gene is necessary for the diagnosis and monitor-ing of CML. Previously, many technologies, most of which are laborious and time consum-ing, have been developed to detect BCR-ABL chimeric gene or chromosome. Methods: A new flow cytometric immunobead assay was used for detection of BCR-ABL fusion pro-teins and applicability, sensitivity, reliability, efficacy and rapidity of this method was evaluated. Results: From February 2009 to January 2014, a total 648 CML patients were investigated for the status of BCR-ABL1 protein. Among them, 83 patients were enrolled for comparative study of BCR-ABL1 positivity by three routinely used procedures like karyotyping, and quantita-tive real time PCR (RT-PCR) as well as immunobead flow cytometry assay. BCR-ABL protein analysis was found consistent, more sensitive (17% greater sensitivity) and reliable than the conventional cytogenetics, as flow cytometry showed 95% concordance rate to RT-PCR. Conclusion: BCR-ABL fusion protein assay using a new flow cytometric immunobead might be useful in the diagnosis and monitoring CML patients.
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Tiwari Jagesh Kumar,Mandadi Nagesh,Sridhar Jandrajupalli,Mandal Vikramjit,Ghosh Arpita,Kardile Hemant B.,Naga Kailash C.,Shah Mohd Abas,Rawat Shashi,Venkateswarlu Vallepu,Malik Kamlesh,Bhatnagar Anuj 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.2
The foxglove aphid (or glasshouse potato aphid, Aulacorthum solani Kaltenbach) transmits serious potato viruses (potato virus Y, and potato leaf roll virus) which cause heavy yield losses. Our aim of this study was to pre liminary analysis of draft genome sequence to uncover virulence genes in the aphid. The genome assembly size (316.39 Mb) was very close to its genome size (318.19 Mb) estimated by flow cytometry. The genome completeness (81.8%) was confirmed by the Benchmarking Universal Single Copy Orthologs (BUSCO) analysis indicating 14.90% transposable elements (TEs) in the genome. Of total 22,021 predicted genes, 16,610 were annotated with putative functions of other aphids mainly Myzus persicae, Acyrthosiphon pisum and Diuraphis noxia. We identified virulence genes such as defensive and detoxification genes, salivary genes and chemore ceptors, insecticide resistance genes, virus transmission genes, transcription factors and mitochondrial genes. Importantly, analysis of detoxification genes particularly 53 cytochrome P450s (CYPs) indicated involvement of 23 CYPs families in aphid genome. Further, GO and KEGG pathways analyses showed gene enrichment pre dominantly with molecular function and signal transduction, respectively. Phylogeny analysis revealed genetic divergence among 12 aphid species and Au. solani is closely related with M. persicae. Further, non-synonymous (Ka)/synonymous (Ks) substitutions (Ka/Ks) indicated positive selection for 6 (Ka/Ks > 1) and 122 (Ka/Ks = 0.5–1) single copy orthologous gene pairs between Au. solani and with the pea aphid. Thus, our preliminary draft genome analysis provides new insights of Au. solani to understand molecular basis of aphid biology, host-aphid interactions and adaptation mechanism.
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