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Yudhistira Nugraha,Sintho Wahyuning Ardie,Munif Ghulammahdi,Suwarno,Hajrial Aswidinnoor 한국작물학회 2016 Journal of crop science and biotechnology Vol.19 No.1
The breeding for iron toxicity tolerant rice needs an effective, efficient, and reliable screening method. The study was aimed to evaluate the best method for screening iron tolerant genotypes at the seedling stage in the greenhouse. Two rice genotypes, Mahsuri (tolerant) and IR64 (sensitive) were grown in three modified media solutions namely, Yoshida-conventional solution (YCS), Yoshida with etylenediamintetraacetic acid (1:2) (YES), and Yoshida with 0.2% agar (YAS). Three levels of iron were tested to observe the severity of their leaf bronzing score (LBS). The optimized solution in the greenhouse was then evaluated using 24 rice genotypes. Using the same genotypes interrelationship, the LBS in the greenhouse with grain yield and its attributes was validated under acute and moderate Fe toxicity in the field. The results showed that the optimized media culture was YAS with 400 mg L-1 of FeSO4. This media had more stable pH and redox potential, it could maintain sufficient Fe2+ supply over 10 days, and it could discriminate of LBS between tolerant and sensitive genotypes. Evaluation using the optimized media solution showed that there was a significant variation among genotypes in shoot dry weight and a significant correlation of relative reduction of shoot dry weight with LBS. The LBS in the greenhouse was correlated with LBS in acute iron stress in the field (r=0.673**) and the grain yield (r= -0.618**). This study has proven that YAS culture media can be used as early identification of iron toxicity tolerant genotypes for supporting breeding programs.
Occurrence and Antibiotic Susceptibility of Listeria Species in Turkey Meats
Zeki Aras,Mustafa Ardiç 한국축산식품학회 2015 한국축산식품학회지 Vol.35 No.5
The aims of this study were to investigate the occurrence of Listeria species in turkey meats and to check the antimicrobial susceptibility of the isolated strains. Hundred and fifteen raw turkey meat samples were randomly collected from the supermarkets, butchers and restaurants. Strain isolation and identification were made according to the ISO11290-1 method. Antimicrobial susceptibility was determined by the standard disc diffusion method. A total of 47 Listeria spp. were isolated from 115 (40.9%) raw turkey meat samples. The isolates were distributed between L. monocytogenes (25.53%), L. innocua (34.04%), L. grayi (31.91%) and L. welshimeri (8.51%). A total of 55.3 % of Listeria spp. isolates were multi-resistant to at least 3 of the antimicrobial agent tested. The level of multi-resistance was higher in L. monocytogenes strains (66.7%) than in L. innocua (62.5%) and L. grayi (53.3%). Listeria spp. isolates were highly resistant to ampicillin, cephalothin, penicillin, meticillin, oxacillin, and trimethoprime-sulfamethoxazole. The isolates particularly L. monocytogenes are increasingly resistant to one or more antibiotics and may represent a potential risk for public health because these antibiotics are common used in treatment of listeriosis. The correct and controlled use of antibiotics in veterinary medicine is important to the emergence of resistant strains.
Generation method of asymmetric chainring design from pedaling torque experimental data
Ardi Lesmawanto,Kao-Kuei Hsu,Shinn-Liang Chang 대한기계학회 2022 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.36 No.12
In cycling, pedaling is a bilateral asymmetries function that related to leg dominance. However, studies about the asymmetric shape on the chainring design considering pedaling asymmetries function and the test are limited. In this paper, a method to generate an asymmetric chainring from the experimental data is proposed. The curve fitting method and the flowchart to synthesize the asymmetric shape from the crank torque data are presented. The experimental test to compare the chainring prototype and the circular chainring on the same number of teeth is conducted. The comparison test is performed by comparing the downstroke torque and power at the same rotational speed on the rear wheel. The result indicates that pedaling on the asymmetric chainring prototype is more efficient and lighter than circular chainring. This work can be used as a guidance procedure to design any asymmetric chainring from the experimental data.