Circle within a Circle: Unique Appearance of Coronary Air Embolism on OCT Imaging

Antara Banerji,Pradyot Tiwari,Tejas Patel,Sanjay Shah 한국심초음파학회 2021 Journal of Cardiovascular Imaging (J Cardiovasc Im Vol.29 No.3

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Effect of graphite particulate on mechanical properties of glass fibre reinforced composite

Bhattacharjee, Antara,Roy, Kanchan,Nanda, B.K. The Society for Aerospace System Engineering 2020 International Journal of Aerospace System Engineer Vol.7 No.1

The recent trend is increasing towards the usage of polymer matrix composites since they have a wide variety of applications. They have applications in the field of aircraft and space industry, sporting goods, medical devices, marine and automotive applications and also in commercial usage. The most commonly used fibre-reinforced polymer matrix composite is Glass fibre reinforced epoxy (GFRE) composite which is used in aviation, sports and automotive industries. However, the strength of GFRE composites is not adequate for structural applications. Therefore, the current research focuses on increasing the strength of GFRE composites by reinforcing with micro Graphite (Gr) particulates. The Gr used is an ultra-fine powder with particle size 250 ㎛. Gr is known to have good wear resistance, thermal conductivity and can operate at high temperatures. Gr particulates are mixed with the epoxy matrix in various weight ratios. Hand-lay technique is used for fabricating the composites. Mechanical properties such as tensile strength, elongation, compressive strength and flexural strength are obtained experimentally to study the effect of change in Gr content (0-5 wt. %). The tests were done as per ASTM standards.

STUDYING LARGE- AND SMALL-SCALE ENVIRONMENTS OF ULTRAVIOLET LUMINOUS GALAXIES

Basu-Zych, Antara R.,Schiminovich, David,Heinis, Sebastien,Overzier, Roderik,Heckman, Tim,Zamojski, Michel,Ilbert, Olivier,Koekemoer, Anton M.,Barlow, Tom A.,Bianchi, Luciana,Conrow, Tim,Donas, Jose,F IOP Publishing 2009 The Astrophysical journal Vol.699 No.2

Thermal characteristics of an ammonia-charged closed-loop pulsating heat pipe

Md Shahidul Haque,Antara Majumdar,Md. Faisal Kader,Md. Mahbubur Razzaq 대한기계학회 2019 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.33 No.4

At this age, engineering applications are demanding effective ways of heat recovery and energy savings for their optimum performance. Among other cooling techniques, pulsating heat pipes have emerged as a convenient and cost effective thermal design solution due to its excellent heat transfer capability, high thermal efficiency and structural simplicity. The paper presents an experimental study on the operational limit of an aluminum closed-loop pulsating heat pipe (CLPHP) charged with ammonia. It consists of total 14 turns of aluminum pipe with 3 mm inner and 4 mm outer diameter. Ammonia was used as working fluid with 3 different filling ratios such as 0.4, 0.6 and 0.8. Operation orientations were vertical, 30°, 45°, 60°, 90° and 180° inclinations. Constant electric heat input of 36 W was applied to the heating block and temperature rise in various sections was monitored till steady state was reached. Temperature was measured at different locations of the CLPHP by using thermocouples. The effects of operational orientations and filling ratios were investigated on heat transfer by working fluid php Q & (Watt), overall heat transfer coefficient U (W/m² °C) and thermal resistance R (°C/W) considering the measured temperature. The result shows that, 0.4 and 0.6 fill ratios and inclination angle of 30º give better result than any other arrangements for CLPHP.

Genomic survey of high-throughput RNA-Seq data implicates involvement of long intergenic non-coding RNAs (lincRNAs) in cytoplasmic male-sterility and fertility restoration in pigeon pea

Saxena Swati,Das Antara,Kaila Tanvi,Ramakrishna G.,Sharma Sandhya,Gaikwad Kishor 한국유전학회 2023 Genes & Genomics Vol.45 No.6

Background Long-intergenic non-coding RNAs (lincRNAs) originate from intergenic regions and have no coding potential. LincRNAs have emerged as key players in the regulation of various biological processes in plant development. Cytoplasmic male-sterility (CMS) in association with restorer-of-fertility (Rf) systems makes it a highly reliable tool for exploring heterosis for producing commercial hybrid seeds. To date, there have been no reports of lincRNAs during pollen development in CMS and fertility restorer lines in pigeon pea. Objective Identification of lincRNAs in the floral buds of cytoplasmic male-sterile (AKCMS11) and fertility restorer (AKPR303) pigeon pea lines. Methods We employed a computational approach to identify lincRNAs in the floral buds of cytoplasmic male-sterile (AKCMS11) and fertility restorer (AKPR303) pigeon pea lines using RNA-Seq data. Results We predicted a total of 2145 potential lincRNAs of which 966 were observed to be differentially expressed between the sterile and fertile pollen. We identified, 927 cis-regulated and 383 trans-regulated target genes of the lincRNAs. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of the target genes revealed that these genes were specifically enriched in pathways like pollen and pollen tube development, oxidative phosphorylation, etc. We detected 23 lincRNAs that were co-expressed with 17 pollen-related genes with known functions. Fifty-nine lincRNAs were predicted to be endogenous target mimics (eTMs) for 25 miRNAs, and found to be associated with pollen development. The, lincRNA regulatory networks revealed that different lincRNA-miRNA-mRNA networks might be associated with CMS and fertility restoration. Conclusion Thus, this study provides valuable information by highlighting the functions of lincRNAs as regulators during pollen development in pigeon pea and utilization in hybrid seed production.

Biodesulfurization of Dibenzothiophene and Its Derivatives Using Resting and Immobilized Cells of Sphingomonas subarctica T7b

( Gunam,Ida Bagus Wayan ),( Kenta Yamamura ),( I Nengah Sujaya ),( Nyoman Semadi Antara ),( Wayan Redi Aryanta ),( Michiko Tanaka ),( Fusao Tomita ),( Teruo Sone ),( Kozo Asano ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.4

Helicobacter pylori increased the γ-glutamyltranspeptidase (GGT) production under low-pH (maximal at pH 4) and appropriate pCO2 conditions, while the production of GGT mRNA correlated with increased total enzyme activity. At pH 4, the bacterium augmented enzyme production in the presence of glutamine (~10 mM) in the medium, which predominantly occurred after a 6-min time-lag. Monovalent salts such as NaCl or NH4Cl facilitated enzymatic activation in acidic solutions of approximately pH 4.5. In addition, glutathione`s γ-glutamyl moiety cysteinylglycine appeared to be taken up readily by the intact H. pylori, but not by the one pretreated with a potent GGT inhibitor, acivicin, suggesting that the GGT may partake in glutathione uptake by the cell.

The Loyalty Model of Foreign Tourists Consuming Traditional Balinese Food

I Gusti Ayu Dewi Hendriyani,Made Budiarsa,Made Antara,Nyoman Sudiarta 사람과세계경영학회 2020 Global Business and Finance Review Vol.25 No.3

Purpose: The purpose of this study is to analyze the effect of meal experience of foreign tourists on satisfaction and loyalty when consuming traditional food in Bali.The choice of traditional Balinese food is traditional food using Balinese spices called Base Genep with the main course category.The purpose of this study also explores the meal experience of tourists consuming traditional food in tourist destinations is expected to provide satisfaction and can lead to loyalty to the destination itself in relation with The Island of Bali as a world tourist destination offers many choices of traditional food for tourists, especially foreign tourists. Design/methodology/approach: This study uses quantitative analysis, structural equation model (SEM-AMOS) which is followed by the interpretation of the research results to answer 3 hypotheses.In building a loyalty model for foreign tourists consumed traditional food in Bali, confirms the concept of meal experience with five dimensions are food quality, service quality, atmosphere, cleanliness and hygiene, and price, which is combined with the Triangle Concept of Indonesian Gastronomy with a storytelling dimension, including from previous research on local culinary in a tourist destination.This study involved 332 respondents using accidental sampling techniques through four culinary tourism activities in Bali namely festival, food tour, cooking class, and homestay, at five locations in Bali province namely Denpasar City, Badung, Gianyar, Bangli, and Buleleng Regency. Findings: The meal experience of foreign tourists consuming traditional Balinese food has a significant effect on tourist satisfaction and also tourist loyalty.And the satisfaction of foreign tourists consuming traditional Balinese food has a positive and significant effect on the loyalty of foreign tourists.The highest direct effect on the effect of meal experience is foreign tourist's satisfaction, then the influence between foreign tourist's satisfaction on foreign tourist's loyalty, and finally the influence between-meal experience and foreign tourist's loyalty.The study finding a new loyalty model for foreign tourists consuming traditional food in Bali destinations with a storytelling indicator as a novelty.The results of this study will provide theoretical implications in enriching consumer behavior theory and product development concepts, as well as further research in the future. Research limitations/implications: The study is due to the respondent only foreign tourists not including domestic tourists, the findings cannot be generalized to the whole population of tourists visiting Bali.Future research can survey tourists domestic and in different areas in Bali to have wider coverage.Meanwhile, the practical implications for culinary stakeholders in Bali are in improving the quality of the tourist's meal experience and in developing culinary tourism in Bali as a whole.

A Comprehensive Cancer-Associated MicroRNA Expression Profiling and Proteomic Analysis of Human Umbilical Cord Mesenchymal Stem Cell-Derived Exosomes

Jothimani Ganesan,Pathak Surajit,Dutta Suman,Duttaroy Asim K.,Banerjee Antara 한국조직공학과 재생의학회 2022 조직공학과 재생의학 Vol.19 No.5

BACKGROUND: The mesenchymal stem cells (MSCs) have enormous therapeutic potential owing to their multi-lineage differentiation and self-renewal properties. MSCs express growth factors, cytokines, chemokines, and non-coding regulatory RNAs with immunosuppressive, anti-tumor, and migratory properties. MSCs also release several anti-cancer molecules via extracellular vesicles, that act as pro-apoptotic/tumor suppressor factors. This study aimed to identify the stem cell-derived secretome that could exhibit anti-cancer properties through molecular profiling of cargos in MSC-derived exosomes. METHODS: Human umbilical cord mesenchymal stem cells (hUCMSCs) were isolated from umbilical cord tissues and culture expanded. Subsequently, exosomes were isolated from hUCMSC conditioned medium and characterized by DLS, electron microscopy. Western blot for exosome surface marker protein CD63 expression was performed. The miRNA profiling of hUCMSCs and hUCMSC-derived exosomes was performed, followed by functional enrichment analysis. RESULTS: The tri-lineage differentiation potential, fibroblastic morphology, and strong expression of pluripotency genes indicated that isolated fibroblasts are MSCs. The isolated extracellular vesicles were 133.8 ± 42.49 nm in diameter, monodispersed, and strongly expressed the exosome surface marker protein CD63. The miRNA expression profile and gene ontology (GO) depicted the differential expression patterns of high and less-expressed miRNAs that are crucial to be involved in the regulation of apoptosis. The LCMS/MS data and GO analysis indicate that hUCMSC secretomes are involved in several oncogenic and inflammatory signaling cascades. CONCLUSION: Primary human MSCs released miRNAs and growth factors via exosomes that are increasingly implicated in intercellular communications, and hUCMSC-exosomal miRNAs have a critical influence in regulating cell death and apoptosis of cancer cells.

MiR-155 modulates the inflammatory phenotype of intestinal myofibroblasts by targeting SOCS1 in ulcerative colitis

Surajit Pathak,Alessia Rosaria Grillo,Melania Scarpa,Paola Brun,Renata D’Incà,Laura Nai,Antara Banerjee,Donatella Cavallo,Luisa Barzon,Giorgio Palù,Giacomo Carlo Sturniolo,Andrea Buda,Ignazio Castagli 생화학분자생물학회 2015 Experimental and molecular medicine Vol.47 No.-

Abnormal levels of microRNA (miR)-155, which regulate inflammation and immune responses, have been demonstrated in the colonic mucosa of patients with inflammatory bowel diseases (IBD), although its role in disease pathophysiology is unknown. We investigated the role of miR-155 in the acquisition and maintenance of an activated phenotype by intestinal myofibroblasts (IMF), a key cell population contributing to mucosal damage in IBD. IMF were isolated from colonic biopsies of healthy controls, ulcerative colitis (UC) and Crohn’s disease (CD) patients. MiR-155 in IMF was quantified by quantitative reverse transcription-PCR in basal condition and following exposure to TNF-α, interleukin (IL)-1β, lipopolysaccharide (LPS) or TGF-β1. The effects of miR-155 mimic or inhibitor transfection on cytokine release and suppressor of cytokine signaling 1 (SOCS1) expression were assessed by enzyme-linked immunosorbent assay and western blot, respectively. Regulation of the target gene SOCS1 expression by miR-155 was assessed using luciferase reporter construct. We found that miR-155 was significantly upregulated in UC as compared with control- and CD-derived IMF. Moreover, TNF-α and LPS, but not TGF-β1 and IL-1β, significantly increased miR-155 expression in IMF. Ectopic expression of miR-155 in control IMF augmented cytokines release, whereas it downregulated SOCS1 expression. MiR-155 knockdown in UC-IMF reduced cytokine production and enhanced SOCS1 expression. Luciferase reporter assay demonstrated that miR-155 directly targets SOCS1. Moreover, silencing of SOCS1 in control IMF significantly increased IL-6 and IL-8 release. In all, our data suggest that inflammatory mediators induce miR-155 expression in IMF of patients with UC. By downregulating the expression of SOCS1, miR-155 wires IMF inflammatory phenotype.