Prevalence and Clinical Impact of IgE-Mediated Food Allergy in School Children With Asthma: A Double-Blind Placebo-Controlled Food Challenge Study

Aneta Krogulska,Jarosław Dynowski,Marzena Funkowicz,Krystyna Wąsowska-Królikowska,Beata Małachowska 대한천식알레르기학회 2015 Allergy, Asthma & Immunology Research Vol.7 No.6

Purpose: Recent studies indirectly suggest a possible link between food allergy (FA) and asthma. Most of them have evaluated the occurrence of FA in asthmatic children, especially in the first year of life, using questionnaire-based studies or specific IgE (sIgE) assay. The aim of this study was to evaluate the prevalence and clinical impact of IgE-mediated FA in school children with asthma using a double-blind placebo-controlled food challenge (DBPCFC). Methods: The study group consisted of school children with atopic asthma who were admitted to the Department of Pediatric Allergology, Gastroenterology and Nutrition, Medical University of Lodz, for the evaluation of food hypersensitivity. The diagnosis of FA was established using questionnaires, sIgE analysis, and the DBPCFC. Asthma severity and asthma control state were also assessed. Results: A relationship between consumed food and complaints was reported in 180 children (49.7%). Seventy children (19.3%) were sensitized to food allergens. IgE-mediated FA was confirmed in 24 children (6.6%), while 11 children (3%) demonstrated respiratory symptoms. Food-induced asthma exacerbations were observed in 9 patients (2.5%). Statistically significant differences in the prevalence of atopic dermatitis (P<0.002), urticaria (P<0.03), digestive symptoms (P<0.03), rhinitis (P<0.02), sIgE level (P<0.001), positive family history of atopy (P<0.001), and FA in history (P<0.001) were found between asthmatic children with FA and those without. Children with food-induced asthma exacerbations demonstrated significantly greater severity, poorer controls, and worse morbidity compared to those without. Conclusions: Although food-induced respiratory reactions in children with asthma were rare, they were classified as severe and associated with worse morbidity, greater severity, and poorer control. As the most commonly observed symptoms were coughing and rhinitis, which can be easily misdiagnosed, a proper diagnosis is essential for improving the management of both clinical conditions.

The Incidence of Delayed-Type Hypersensitivity Reactions to Apples Among Patients Allergic to Birch Pollen

Aneta Wagner,Hanna Zielińska-Bliźniewska,Waldemar Wagner 대한천식알레르기학회 2018 Allergy, Asthma & Immunology Research Vol.10 No.4

The major apple allergen Mal d 1 cross-reacts with the homologous birch pollen allergen Bet v 1 and causes immunoglobulin E (IgE)-mediated immediate- type allergic reactions. In some patients, delayed-type hypersensitivity to apples may develop within 72 hours without evidence of specific IgE or a positive skin prick test (SPT). The aim of the study was to evaluate the concomitance of delayed-type hypersensitivity reactions and immediate IgE-mediated reactions against high- and low-allergenic apple cultivars in patients with birch pollen allergy. Data were obtained from 45 adults with clinical symptoms of birch pollen allergy. Patients were exposed to apple pulp via atopy patch tests (APTs) and SPTs. Levels of IgE specific to Bet v 1 and Mal d 1 were measured with a radioallergosorbent test. Patients allergic to birch pollen showed the highest rate of positive SPT responses to Golden Delicious apples and the lowest rate to low-allergenic cultivar Grey French Reinette. Among these patients, 9% developed delayed hypersensitivity reactions to either Golden Delicious or Grey French Reinette apples; these reactions manifested clinically as erythema with papules (class ++). Fifty percent of APT-positive patients were concomitantly SPT-negative. Here, we show for the first time the clinical relevance of T cell-driven allergic reactions to apples. APTs may reveal type IV sensitization in patients who are negative for the corresponding type I sensitization tests. Thus, utilization of the APT procedure with fresh apple appears to be a valuable tool for the diagnosis of apple allergy and may improve the accuracy of food allergy diagnoses.

VISUAL PRESENTATION FORMATS & ONLINE SHOPPING EXPERIENCES

Aneta Geistova,Kirk Plangger 글로벌지식마케팅경영학회 2016 Global Marketing Conference Vol.2016 No.7

Many consumers are buying products online but a key barrier to purchase are insufficiently detailed product demonstrations (Rose et al., 1999). Consumers have different expectations of online shopping for various product categories sold on the Internet (Hyllegard et al., 2000). The purchase of physical goods, such as sofas, wardrobes or tables, is often associated with increased risk and presents a problem for large furniture retailers, such as Habitat or IKEA. These type of goods be characterized as infrequent and expensive purchases that require extensive decision-making (Oh, Yoon and Shyu, 2008). Furthermore, almost 78% of online shoppers abandon their shopping carts because of uncertainty about the consequences of the purchase (Goldwyn, 2003). Currently, large home-furnishing retailers have only used static product presentation formats for their products online that offer basic product information. Thus, this study investigates different product presentation formats that could be used by these retailers in an experimental setting.

Myocardial Infarction Type 4b in Human Immunodeficiency Virus-Infected Patient

Aneta Cybula-Walczak,Filip M. Szymanski,Anna E. Platek,Grzegorz Karpinski 대한심장학회 2014 Korean Circulation Journal Vol.44 No.1

We report a case of a 52-year-old human immunodeficiency virus (HIV)-infected male patient receiving combined antiretroviral therapy(cART), who presented with acute ST-elevation myocardial infarction (STEMI). He was properly treated (e.g., prescribed anti-coagulationdrugs: aspirin, clopidogrel, enoxaparin) and discharged. After 1.5 months, another STEMI related with in-stent thrombosis took place. ThecART scheme was altered, resulting in no further cardiac events in the follow-up period, with undetectable levels of HIV ribonucleic acid. This case highlights the association between HIV infection and the specific drugs of cART, and the risk of cardiovascular disease develop -ment.

Comparative scanning electron microscope analysis of the enamel of permanent human, bovine and porcine teeth

Aneta Olek,Leszek Klimek,Elżbieta Bołtacz-Rzepkowska 대한수의학회 2020 Journal of Veterinary Science Vol.21 No.6

Background: Bovine and porcine teeth are often used in in vitro experiments as substitutes of human teeth. Objectives: The aim of the present study was to perform a comparative analysis of enamel morphology of permanent human, bovine and porcine teeth under the scanning electron microscope. Methods: As many as 10 human, 10 bovine, and 10 porcine teeth were studied. All the teeth were sectioned and the halves were randomly divided into 2 groups according to the examined tissue (vestibular enamel at the mid-height of the dental crown and in the cervical area). Human and bovine enamel was etched for 15 sec and porcine enamel for 30 sec. The scanning electron microscope analysis was performed. The length and width of enamel prisms were determined with the “Met-Ilo” 1.1 computer program. Results: All enamel samples revealed the same etching pattern—Silverstone's type 2. Bovine enamel showed a similar porosity and the amount of interprismatic enamel compared to human enamel while the amount and width of interprismatic enamel bands in porcine enamel were evidently greater. The shape of the porcine prisms was visually similar to human prisms, although dimensions were significantly different. However, bovine prisms differed in form and appeared to be distinctly elongated. Conclusions: Reported findings indicate that the results of experimental studies carried out on bovine and porcine enamel should not be compared with the results obtained on human enamel.

Deep Borehole Heat Exchangers — A Conceptual and Comparative Review

Aneta Sapinska-Sliwa,Marc A. Rosen,Andrzej Gonet,Tomasz Sliwa 대한설비공학회 2016 International Journal of Air-Conditioning and Refr Vol.24 No.1

Borehole heat exchangers (BHEs) are used for transforming a rock mass into an underground heat storage. Usually, their depth does not exceed 200 m, but some extend to a depth of almost 3000 m. Underground heat storages can operate as part of heating and cooling systems, often economically. In winter they extract heat from the rock mass for space heating, while in summer the cooled rock mass is used for air conditioning. The heat extracted from buildings via air conditioning is transferred into the rock mass, thereby regenerating its condition for winter time. Deep borehole exchangers also may operate only in the heating mode. Then, the rock resource conditions are regenerated via heat transfer through neighboring rocks. If a groundwater °ow is present, the heat can also be removed and the source conditions regenerated through convection. Here, an overview of the use and operation of deep BHEs around the world is provided. Special emphasis is placed on the Carpathians, where numerous analyses of geothermal heat use have been performed since 1999. Examples of calculations for old oil and gas wells aswell as negative exploration boreholes are given. Such analyses have been performed for boreholes in Poland and the Ukraine. However, little research has been published on this subject to date, for reasons described herein.

Effects of Epothilone A in Combination with the Antidiabetic Drugs Metformin and Sitagliptin in HepG2 Human Hepatocellular Cancer Cells: Role of Transcriptional Factors NF-κB and p53

Rogalska, Aneta,Sliwinska, Agnieszka,Kasznicki, Jacek,Drzewoski, Jozef,Marczak, Agnieszka Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.3

Type 2 diabetes mellitus patients are at increased risk of many forms of malignancies, especially of the pancreas, colon and hepatocellular cancer. Unfortunately, little is known of the possible interaction between antidiabetic drugs and anticancer agents. The present study investigates the influence of metformin (MET) and sitagliptin (SITA) on the in vitro anticancer activity of the microtubule depolymerization inhibitor agent epothilone A (EpoA). Hepatocellular liver carcinoma cell line (HepG2) viability and apoptosis were determined by the MTT test and by double staining with PO-PRO-1 and 7-aminoactinomycin D, respectively, after treatment with EpoA, metformin or sitagliptin. The levels of nuclear factor NF-${\kappa}B$ and p53 were evaluated in the presence and absence of inhibitors. While EpoA and MET inhibited HepG2 cell proliferation, SITA did not. EpoA and SITA induced higher p53 levels than MET. All tested drugs increased the level of NF-${\kappa}B$. Only MET enhanced the proapoptotic effect of EpoA. The EpoA+MET combination evoked the highest cytotoxic effect on HepG2 cells and led to apoptosis independent of p53, decreasing the level of NF-${\kappa}B$. These findings support the link between NF-${\kappa}B$ and p53 in the modulation of apoptotic effects in HepG2 cells treated by EpoA. Our studies indicate that the combination of EpoA and MET applied in subtoxic doses has a stronger cytotoxic effect on liver cancer cells than each of the compounds alone. The therapeutic advantages of the combination of EpoA with MET may be valuable in the treatment of patients with diabetes mellitus type 2 (T2DM) and liver cancer.

Nuclear DNA Damage and Repair in Normal Ovarian Cells Caused by Epothilone B

Rogalska, Aneta,Marczak, Agnieszka Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.15

This study was designed to assess, whether a new chemotherapeutic microtubule inhibitor, Epothilone B (EpoB, Patupilone), can induce DNA damage in normal ovarian cells (MM14.Ov), and to evaluate if such damage could be repaired. The changes were compared with the effect of paclitaxel (PTX) commonly employed in the clinic. The alkaline comet assay technique and TUNEL assay were used. The kinetics of DNA damage formation and the level of apoptotic cells were determined after treatment with IC50 concentrations of EpoB and PTX. It was observed that PTX generated significantly higher apoptotic and genotoxic changes than EpoB. The peak was observed after 48 h of treatment when the DNA damage had a maximal level. The DNA damage induced by both tested drugs was almost completely repaired. As EpoB in normal cells causes less damage to DNA it might be a promising anticancer drug with potential for the treatment of ovarian tumors.

MMP3 in Comparison to CA 125, HE4 and the ROMA Algorithm in Differentiation of Ovarian Tumors

Cymbaluk-Ploska, Aneta,Chudecka-Glaz, Anita,Surowiec, Anna,Pius-Sadowska, Ewa,Machalinski, Boguslaw,Menkiszak, Janusz Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.5

Ovarian cancer is a highly malignant neoplasm with high mortality rates. Research to identify markers facilitating early detection has been pursued for many years. Currently, diagnosis is based on the CA 125 and HE4 markers, as well as the ROMA algorithm. The search continues for new proteins that meet the criteria of good markers A total of 90 patients were included in the present study, allocated into: group 1, ovarian cancer, with 29 patients; group 2, endometrial cysts, with 30s; and group 3, simple ovarian cysts, with 31. Following histopathological verification, the CA 125, HE4, and metalloproteinase 3 (MMP3) levels were determined and the ROMA algorithm was calculated for all patients. The mean concentrations of all determined proteins, CA 125, HE4, and MMP3, as well as the ROMA values, were significantly higher in group 1 (ovarian cancer) compared to group 3 (simple ovarian cysts). The highest significant differences for the CA 125 levels (p<0.000001) and ROMA (p<0.000001) values were observed in postmenopausal women. For HE4, statistical significance was at the level of p=0.00001 compared to p=0.002 for MMP3. For the differentiation between ovarian cancer and endometrial cysts, the respective AUC ratios were obtained for CA 125, HE4, and MMP3 levels, as well as the ROMA values ( 0,93 / 0,96 / 0,75 / 0,98). After removing the post-menopausal patients, the MMP3 AUC value for ovarian cancer vs. benign ovarian cysts increased to 0.814. For post-menopausal women, the MMP3 AUC value for ovarian cancer vs. endometrial cysts was 0.843. As suggested by the results above, both the CA 125 and HE4 markers, as well as the ROMA algorithm, meet the criteria of a good diagnostic test for ovarian cancer. MMP3 seems to meet the criteria of a good diagnostic test, particularly in postmenopausal women; however, it is not superior to the tests used to date.

Poster Session:PS 0210 ; Family Medicine : Stagnating and Low Infl uenza Vaccine Coverage among Polish Elderly in 2008-2012

( Ewa Gyrczuk ),( Aneta Nitsch Osuch ),( Katarzyna Zycinska ),( Kazimierz Wardyn ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: Despite having no higher attack rate than in younger adults, infl uenza`s effects are more signifi cant in older adults. It has been recognized for many years that people 65 years and older are at greater risk of serious complications from infl uenza compared with young, healthy adults. There is a limited literature on the vaccination coverage rates in Central Eastern European countries, including Poland. The aim of the study was to describe the infl uenza vaccine coverage among the Polish elderly aged > 65 years in a fi ve years period 2008-2012- in a general population and in a single primary care setting. Methods: The retrospective analysis of infl uenza vaccine coverage among patients older than 65 years from a single primary care clinic was conducted. The payment of infl uenza vaccine for the elderly was provided by a local goverment. The inactivated trivalent split vaccine was used for all vaccinated patients. Patients were informed about the free infl uenza vaccines by standing posters at the clinic. Age and sex distribution of patients who decided to be vaccinated against infl uenza was described as well as the willingness to repeat the vaccination during consecutive seasons. The vaccine coverage rate was calculated as a percentage of vaccinated individuals among all patients over the age of 65 years. Results: In the analyzed period, 2008-2012, the infl uenza vaccine coverage among the elderly in a general Polish population ranged from 7,6% (in 2012 ) to 11,3% (in 2009). In a single primary care setting the infl uenza vaccine coverage rate among seniors was the highest one in 2010 (31,56%) and the lowest one in 2012 (21%). Generally, less than 50% of vaccine doses were given every year to the elderly. Conclusions: Infl uenza vaccine coverage among Polish elderly is on stagnating low level.