Salivary soluble receptor activator of nuclear factor kappa B ligand/osteoprotegerin ratio in periodontal disease and health

Zahra Alizadeh Tabari,Abbas Azadmehr,Mohammad Amir Alizadeh Tabrizi,Jalaloddin Hamissi,Fatemeh Baharak Ghaedi 대한치주과학회 2013 Journal of Periodontal & Implant Science Vol.43 No.5

Purpose: The receptor activator of nuclear factor kappa B (RANK)/RANK ligand (RANKL)/osteoprotegerin (OPG) system plays a significant role in osteoclastogenesis, activation of osteoclasts, and regulation of bone resorption. This study aimed to evaluate the use of the salivary soluble RANKL (sRANKL)/OPG ratio as a diagnostic marker for periodontitis in nonsmokers. Methods: Twenty-five patients with chronic periodontitis and 25 individuals with a healthy periodontium were enrolled in this study. Samples containing 5 mL of unstimulated saliva were obtained from each subject. Salivary sRANKL and OPG concentrations were determined using a standard enzyme-linked immunosorbent assay. Statistical analysis was performed using SPSS ver. 18.0. Results: The levels of sRANKL and OPG were detectable in all of the samples. Positive relationships were found between the plaque index and clinical attachment level and both the salivary concentration of sRANKL and the salivary sRANKL/OPG ratio (P<0.05). The salivary concentration of sRANKL and the sRANKL/OPG ratio were significantly higher in the periodontitis group than in the healthy group (P=0.004 and P=0.001, respectively). In contrast, the OPG concentration showed no significant differences between the groups (P=0.455). Conclusions: These findings suggest that the salivary sRANKL/OPG ratio may be helpful in the screening and diagnosis of periodontitis. However, longitudinal studies with larger populations are needed to confirm these results. Purpose: The receptor activator of nuclear factor kappa B (RANK)/RANK ligand (RANKL)/osteoprotegerin (OPG) system plays a significant role in osteoclastogenesis, activation of osteoclasts, and regulation of bone resorption. This study aimed to evaluate the use of the salivary soluble RANKL (sRANKL)/OPG ratio as a diagnostic marker for periodontitis in nonsmokers. Methods: Twenty-five patients with chronic periodontitis and 25 individuals with a healthy periodontium were enrolled in this study. Samples containing 5 mL of unstimulated saliva were obtained from each subject. Salivary sRANKL and OPG concentrations were determined using a standard enzyme-linked immunosorbent assay. Statistical analysis was performed using SPSS ver. 18.0. Results: The levels of sRANKL and OPG were detectable in all of the samples. Positive relationships were found between the plaque index and clinical attachment level and both the salivary concentration of sRANKL and the salivary sRANKL/OPG ratio (P<0.05). The salivary concentration of sRANKL and the sRANKL/OPG ratio were significantly higher in the periodontitis group than in the healthy group (P=0.004 and P=0.001, respectively). In contrast, the OPG concentration showed no significant differences between the groups (P=0.455). Conclusions: These findings suggest that the salivary sRANKL/OPG ratio may be helpful in the screening and diagnosis of periodontitis. However, longitudinal studies with larger populations are needed to confirm these results.

Salivary soluble receptor activator of nuclear factor kappa B ligand/osteoprotegerin ratio in periodontal disease and health

Tabari, Zahra Alizadeh,Azadmehr, Abbas,Tabrizi, Mohammad Amir Alizadeh,Hamissi, Jalaloddin,Ghaedi, Fatemeh Baharak Korean Academy of Periodontology 2013 Journal of Periodontal & Implant Science Vol.43 No.5

Purpose: The receptor activator of nuclear factor kappa B (RANK)/RANK ligand (RANKL)/osteoprotegerin (OPG) system plays a significant role in osteoclastogenesis, activation of osteoclasts, and regulation of bone resorption. This study aimed to evaluate the use of the salivary soluble RANKL (sRANKL)/OPG ratio as a diagnostic marker for periodontitis in nonsmokers. Methods: Twenty-five patients with chronic periodontitis and 25 individuals with a healthy periodontium were enrolled in this study. Samples containing 5 mL of unstimulated saliva were obtained from each subject. Salivary sRANKL and OPG concentrations were determined using a standard enzyme-linked immunosorbent assay. Statistical analysis was performed using SPSS ver. 18.0. Results: The levels of sRANKL and OPG were detectable in all of the samples. Positive relationships were found between the plaque index and clinical attachment level and both the salivary concentration of sRANKL and the salivary sRANKL/OPG ratio (P<0.05). The salivary concentration of sRANKL and the sRANKL/OPG ratio were significantly higher in the periodontitis group than in the healthy group (P=0.004 and P=0.001, respectively). In contrast, the OPG concentration showed no significant differences between the groups (P=0.455). Conclusions: These findings suggest that the salivary sRANKL/OPG ratio may be helpful in the screening and diagnosis of periodontitis. However, longitudinal studies with larger populations are needed to confirm these results.

Shape and Orifice Optimization of Airbag Systems for UAV Parachute Landing

Alizadeh, Masoud,Sedaghat, Ahmad,Kargar, Ebrahim The Korean Society for Aeronautical and Space Scie 2014 International Journal of Aeronautical and Space Sc Vol.15 No.3

An airbag is an important safety system and is well known as a safety system in cars during an accident. Airbag systems are also used as a shock absorber for UAVs to assist with rapid parachute landings. In this paper, the dynamics and gas dynamics of five airbag shapes, cylindrical, semi-cylindrical, cubic, and two truncated pyramids, were modelled and simulated under conditions of impact acceleration lower than $4m/s^2$ to avoid damage to the UAV. First, the responses of the present modelling were compared and validated against airbag test results under the same conditions. Second, for each airbag shape under the same conditions, the responses in terms of pressure, acceleration, and emerging velocity were investigated. Third, the performance of a pressure relief valve is compared with a fixed-area orifice implemented in the air bag. For each airbag shape under the same conditions, the optimum area of the fixed orifice was determined. By examining the response of pressure and acceleration of the airbag, the optimum shape of the airbag and the venting system is suggested.

A new hydrogen cyanide chemiresistor gas sensor based on graphene quantum dots

Alizadeh, Taher,Shokri, Mahrokh,Hanifehpour, Younes,Joo, Sang Woo Taylor Francis 2016 International journal of environmental analytical Vol.96 No.8

<P>Graphene quantum dot (GQDs), synthesised via controlled carbonisation of citric acid, were reduced by hydrazine hydrate and then used as hydrogen cyanide (HCN) gas sensors. Checking of the reduction step by Fourier transform infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS) techniques revealed that most of the oxygen-containing functional groups were removed from the GQDs. It was observed the reduction process is necessary for sensitising of GQDs for HCN gas. The electrical resistance of the reduced GQDs was increased as a result of their exposure to HCN gas. Accepting a p-type semiconducting characteristic for GQD material, he above-mentioned behaviour suggested electron donation from HCN to GQD. The sensor response to HCIsil gas was reversible, suggesting a reversible adsorption/desorption phenomenon of HCN to the GQDs. The response as well as the recovery time of the sensor was different depending on the HCN concentration tested. The developed sensor showed linear HCN response from 1 to 100 ppm. The detection limit of the sensor was estimated to be 0.6 ppm (SIN). Relative standard deviation f HCN determination by the developed sensor was calculated to be 5.7% (n = 4, [HCN]= 50 ppm). The sensor response was did not vary significantly within 6 months</P>

Adsorption of methylene blue from an aqueous dyeing solution by use of santa barbara amorphous-15 nanostructure: Kinetic and isotherm studies

Alizadeh, Reza,Zeidi, Amir Techno-Press 2017 Advances in environmental research Vol.6 No.2

Santa Barbara Amorphous-15(SBA-15) nanoparticles were utilized as the inexpensive and effective adsorbents to remove methylene blue dye from the aqueous solution.SBA-15 was created by Zhao et al method. Infrared spectroscopy, X-ray diffraction and scanning electron microscopy (SEM) were used for the evaluated physical properties of SBA-15. The results of diffraction X-ray indicated that was the crystalline structure for it. Also IR spectroscopy indicated was a silica the whole structure of the groups and SEM image verify the structure of relatively identical particles size of SBA-15. Factors affecting adsorption including the amounts of adsorbent, pH and contact time were investigated by a SBA-15 nanomaterial design. The extent of dye removal enhanced with increasing initial dye concentration and pH from 4 to 10. The higher percentage adsorption were obtained under optimum conditions of variables (sorbent dose of 200 mg/liter, initial MB concentration 10 mg/liter, initial pH of 10 and temperature of $25^{\circ}C$). Maximum adsorption happened after the 2 hour and the kinetic processes of the dyes adsorption were described by applying the pseudo-first-order and the pseudo-second-order and the relatively High correlation with the kinetic Ellovich models. It was found that the pseudo-second-order models kinetic equation described the data of dye adsorption with a good correlation (R2>0.999) which indicated chemisorption mechanism. Freundlich and Langmuir adsorption models were investigated in conditions of variables (adsorbent dose 0.01 gr/liter, MB concentration 10, 20, 30 mg/liter, pH of 4, 7, 10, contact time 90 min and temperature of $27^{\circ}C$). The adsorption data were represented by Langmuir isotherm model. These values are higher than the adsorption capacities of some other adsorbents that have recently been published in the literature.

Fumonisin B1 Contamination of Cereals and Risk of Esophageal Cancer in a High Risk Area in Northeastern Iran

Alizadeh, Ali Mohammad,Roshandel, Gholamreza,Roudbarmohammadi, Shahla,Roudbary, Maryam,Sohanaki, Hamid,Ghiasian, Seyed Amir,Taherkhani, Amir,Semnani, Shahryar,Aghasi, Maryam Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.6

Introduction: Fumonisin B1 (FB1) is a toxic and carcinogenic mycotoxin produced in cereals due to fungal infection. This study was conducted to determine FB1 contamination of rice and corn samples and its relationship with the rate of esophageal cancer (EC) in a high risk area in northeastern Iran. Methods: In total, 66 rice and 66 corn samples were collected from 22 geographical subdivisions of Golestan province of Iran. The levels of FB1 were measured for each subdivision by thin layer and high pressure liquid chromatographies. The mean level of FB1 and the proportions of FB1 contaminated samples were compared between low and high EC-risk areas of the province. Results: The mean of FB1 levels in corn and rice samples were 223.64 and 21.59 ${\mu}g/g$, respectively. FB1 contamination was found in 50% and 40.9% of corn and rice samples, respectively. FB1 level was significantly higher in rice samples obtained from high EC-risk area (43.8 ${\mu}g/g$) than those obtained from low risk area (8.93 ${\mu}g/g$) (p-value=0.01). The proportion of FB1 contaminated rice samples was also significantly greater in high (75%) than low (21.4%) EC-risk areas (p-value=0.02). Conclusion: We found high levels of FB1 contamination in corn and rice samples from Golestan province of Iran, with a significant positive relationship between FB1 contamination in rice and the risk of EC. Therefore, fumonisin contamination in commonly used staple foods, especially rice, may be considered as a potential risk factor for EC in this high risk region.

Genetic Diversity of Fusarium proliferatum Populations from Maize, Onion, Rice and Sugarcane in Iran Based on Vegetative Compatibility Grouping

Alizadeh, Alireza,Javan-Nikkhah, Mohammad,Fotouhifar, Khalil-Berdi,Motlagh, Elahe Rabiee,Rahjoo, Vahid The Korean Society of Plant Pathology 2010 Plant Pathology Journal Vol.26 No.3

Fusarium proliferatum is the causal agent of stalk and root rot disease of maize, foot rot disease of rice, basal and root rot disease of onion and knife cut disease of sugarcane in Iran. In recent years, incidence and severity of these diseases have been increased in Iran. Fifty seven F. proliferatum single-spore isolates collected from diseased maize, rice, onion and sugarcane plants at different areas were used to study genetic diversity by determination of vegetative compatibility groups (VCGs). Chlorate-resistant nitrate non-utilizing (nit) mutants were recovered from selected isolates of F. proliferatum and used in complementation tests. All isolates in which both nit1 and NitM (or nit3) mutants were recovered, demonstrated self-compatibility. Vegetative compatibility tests by pairing nit mutants identified 30 VCGs among 57 isolates. Twenty-three isolates belonged to singlemember VCGs and the remaining 34 isolates, belonged to other seven multimember VCGs. Segregation of F. proliferatum isolates obtained from various area and host plants into different VCGs in Iran is reported for the first time. In this study, none of isolates obtained from rice complemented with any other isolates from onion and sugarcane and, non complementation occurred between onion and sugarcane isolates. Also, only one complementation occurred between one isolate of maize and one isolate of sugarcane and rice. Thus, a correlation between VCGs grouping and host preferences was founded. It is concluded that natural populations of F. proliferatum in Iran are probably genetically divergent and include isolates representing a potential risk for disease development.

Tests of fit for the Laplace distribution based on correcting moments of entropy estimators

Alizadeh Noughabi, Hadi,Park, Sangun Taylor Francis 2016 JOURNAL OF STATISTICAL COMPUTATION AND SIMULATION Vol.86 No.11

Effect of First Line Gastric Cancer Chemotherapy Regime on the AGS Cell Line - MTT Assay Results

Alizadeh-Navaei, Reza,Rafiei, Alireza,Abedian-Kenari, Saeid,Asgarian-Omran, Hossein,Valadan, Reza,Hedayatizadeh-Omran, Akbar Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.1

Background: Combination chemotherapy regimes are common treatments for cancer. The aim of this study was to evaluation the effect of individual chemotherapeutic agents in comparison with a first line chemotherapy regime treatment in the AGS gastric cancer cell line by MTT assay. Materials and Methods: In this experimental study, AGS cells were grown in RPMI-1640 supplemented with 10% fetal calf serum and 100 IU/ml penicillin, and $10{\mu}g/ml$ streptomycinin, under a humidified condition at $37^{\circ}C$ with 5% CO2. All cells were washed with PBS and detached with trypsin, centrifuged and 8000 cells re-plated on to 96- well plates. LD50 doses of Epirubicin, Cisplatin and 5-fluorouracil were added to each well in mono or triple therapy. Anti-proliferative activities were determined by MTT assay after 24, 48 or 72 h. Results: Results of MTT assays showed that there were no significant differences among 3 drugs in monotherapy (p=0.088), but there was significant difference between combination therapy with epirubicin (P=0.031) and 5FU (p=0.013) on cell survival at 24 h. After 48 and 72 hours, cell viability showed significant differences between the 3 drugs (p=0.048 and P=0.000 for 48 and 72 h, respectively) and there was significant difference between combination therapy with epirubicin (P=0.035 and P=0.002 for 48 and 72 h, respectively). Conclusions: The results showed no significant differences between these chemotherapy drugs each given alone, but combination therapy with 3 drugs had significant effects on cell viability in comparison with epirubicin alone.

WEAK CONVERGENCE THEOREMS FOR 2-GENERALIZED HYBRID MAPPINGS AND EQUILIBRIUM PROBLEMS

Alizadeh, Sattar,Moradlou, Fridoun Korean Mathematical Society 2016 대한수학회논문집 Vol.31 No.4

In this paper, we propose a new modied Ishikawa iteration for finding a common element of the set of solutions of an equilibrium problem and the set of fixed points of 2-generalized hybrid mappings in a Hilbert space. Our results generalize and improve some existing results in the literature. A numerical example is given to illustrate the usability of our results.