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- 저자 : AlShebly (검색결과 3 건)
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Fatma Saeed AlQahtani,Mashael Marzouq AlShebly,Marimuthu Govindarajan,Sengamalai Senthilmurugan,Periasamy Vijayan,Giovanni Benelli 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.1
Botanical-based nanosynthesis has been recently reported as a cheap alternative for mosquito management. In this study, we investigated the synthesis of silver nanoparticles (AgNPs) using the leaf extract of Rubus ellipticus and AgNPswere characterized using UV–vis spectroscopy, XRD, FTIR, SEM, TEMand EDX analyses. The toxicity of R. ellipticus leaf extract and AgNPs was evaluated against eggs, larvae and adults of Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus. Compared to the leaf aqueous extract, biosynthesized AgNPs showed higher toxicity against the mosquito larvae with LC50 values of 12.50, 13.83 and 15.09 μg/mL, respectively. AgNPs exerted zero hatchability at 60, 75 and 90 μg/mL on A. stephensi, A. aegypti, and C. quinquefasciatus, respectively. Concentrations of 12–60 μg/mL reduced egg laying by gravid females from72.28 to 91.48%.Maximumadulticidal efficacywas observed for AgNPs,with LD50 values were 21.10, 23.04 and 25.06 μg/mL, respectively. AgNPs were found safer to non-target organisms Anisops bouvieri, Diplonychus indicus and Gambusia affinis, with LC50 values ranging from 896 to 2261 μg/mL. Overall, R. ellipticus-fabricated AgNPs acted as effective toxic agents and ovideterrents against three important mosquitoes species, with little harmful effects on non-target aquatic species.
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Biochemical Markers of Oxidative Stress in Saudi Women with Recurrent Miscarriage
Hazem K. Ghneim,Mashael M. Alshebly 대한의학회 2016 Journal of Korean medical science Vol.31 No.1
This study was undertaken to investigate the antioxidant/oxidant status in recurrent miscarriage patients. Antioxidants including glutathione peroxidase (GPx), catalase (CAT), glutathione reductase (GR), reduced glutathione (GSH) and selenium (Se), as well as the oxidants hydrogen peroxide (H2O2), oxidised glutathione (GSSG) and lipid peroxidation were assayed in plasma, whole blood and placental tissue of non-pregnant women (NP), healthy pregnant women (HP), and recurrent miscarriage (RM) patients. Results indicated that all antioxidant activities and levels in plasma and whole blood of HP women were consistently moderately lower, and much more significantly lower in RM patients when both were compared to those seen in NP women (P < 0.05 and P < 0.001, respectively). Furthermore, whereas plasma antioxidant activities and levels were significantly lower in RM patients, those of whole blood and placental tissue were much more significantly lower when compared with HP women (P < 0.001). Concurrent with these findings there were consistent increases of equal statistical significance and magnitude in the levels of all investigated oxidants assayed in all samples when compared in between subjects of the study as indicated above. Data thus illustrated a distinct shift in favor of oxidative reactions and reactive oxygen species (ROS) generation, and very significant decreases in the GSH/ GSSG ratios in whole blood and placental tissue of RM patients when compared to HP and NP women (P < 0.001). The above noted oxidative stress could have been a major causative factor of recurrent miscarriage.
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Raja Mohamed Sait Thameem Azarudeen,Marimuthu Govindarajan,Mashael Marzouq AlShebly,Fatma Saeed AlQahtani,Abubucker Amsath,Sengamalai Senthilmurugan,Periasamy Vijayan,Giovanni Benelli 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.2
Mosquito-borne diseases represent a major human and animal health problem in all tropical and subtropical countries worldwide. The synthesis of eco-friendly metal nanoparticles is a fast-growing branch of current nanoscience with a number of biomedical and entomological implications. In this study, we investigated the one-pot synthesis of silver nanoparticles (Ag NPs) using a cheap leaf extract of Merremia emarginata (Convolvulaceae). Bio-reduced Ag NPs were characterized by UV–visible spectrophotometry, Fourier transform infrared spectroscopy, X-ray diffraction analysis, atomic force microscopy, scanning electron microscopy and transmission electron microscopy. SEM, TEM and AFM morphological nanocharacterization showed that this synthesis led to the production of size-controlled Ag NPs, ranging from 25 to 65 nm. The acute toxicity of M. emarginata leaf extract and biosynthesized Ag NPs was evaluated against third instar larvae of the malaria vector Anopheles stephensi, the dengue and Zika virus vector Aedes aegypti and the filariasis vector Culex quinquefasciatus. Compared to the leaf aqueous extract, Ag NPs showed higher toxicity against A. stephensi, A. aegypti, and C. quinquefasciatus with LC50 values of 8.36, 9.20 and 10.02 μg/mL, respectively. M. emarginata-fabricated Ag NPs were found safer to non-target aquatic biocontrol agents Anisops bouvieri, Diplonychus indicus and Gambusia affinis, with LC50 ranging from 415.61 to 25,153.46 μg/mL. However, although it has been proven the AgNPs fabricated in the present study are more effective if compared to the crude M. emarginata extract, they still exert some toxicity on biocontrol agents, thus further research is required to produce more eco-friendly formulations for field purposes.
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