Do Women over 35 Years Old Who Have Undergone a Myomectomy Require More Acupuncture Sessions to Become Pregnant?

Ajayi, Abayomi B,Ajayi, Tola R,Ejeliogu, Iniobong S,Ajayi, Victor D,Afolabi, Bamgboye M Korean AcupunctureMoxibustion Medicine Society 2018 Korean Journal of Acupuncture Vol.35 No.4

Background: To evaluate whether ${\geq}3$ adjunct acupuncture sessions accompanying embryo transfer, increases the chance of pregnancy amongst post-myomectomy women aged ${\geq}35$ years. Methods: This was a prospective study carried out at Nordica Fertility Center. Following written informed consent, 75 patients undergoing assisted reproduction therapy and who had good quality embryos, were age-matched and grouped into post-myomectomy (n = 24) and normal women who had no evidence of fibroids or previous myomectomy (n = 51). Between 1 and 3 sessions of acupuncture were performed on 6 post-myomectomy and 19 infertile women who had not undergone myomectomy, while > 3 acupuncture sessions were performed on 18 post-myomectomy and on 32 normal patients, approximately 25 minutes before and after embryo transfer. Results: A positive pregnancy test was defined as ultrasonographic evidence indicating presence of a fetal sac 6 weeks after embryo transfer. Of the 5 post-myomectomy women who were pregnant, only 1 (20.0%) received 1-3 adjunct acupuncture sessions whilst the remaining 4 (80.0%) received > 3 acupuncture sessions. Of the 11 normal pregnant women, 5 (45.4%) received 1-3 adjunct acupuncture sessions while 6 (54.5%) received > 3 adjunct acupuncture sessions. Conclusion: Pregnancy rates in infertile post-myomectomy women may be improved by > 3 adjunct acupuncture sessions.

Do Women over 35 Years Old Who Have Undergone a Myomectomy Require More Acupuncture Sessions to Become Pregnant?

Abayomi B Ajayi,Tola R Ajayi,Iniobong S Ejeliogu,Victor D Ajayi,Bamgboye M Afolabi 대한침구의학회 2018 대한침구의학회지 Vol.35 No.4

Background: To evaluate whether ≥ 3 adjunct acupuncture sessions accompanying embryo transfer, increases the chance of pregnancy amongst post-myomectomy women aged ≥ 35 years. Methods: This was a prospective study carried out at Nordica Fertility Center. Following written informed consent, 75 patients undergoing assisted reproduction therapy and who had good quality embryos, were agematched and grouped into post-myomectomy (n = 24) and normal women who had no evidence of fibroids or previous myomectomy (n = 51). Between 1 and 3 sessions of acupuncture were performed on 6 postmyomectomy and 19 infertile women who had not undergone myomectomy, while > 3 acupuncture sessions were performed on 18 post-myomectomy and on 32 normal patients, approximately 25 minutes before and after embryo transfer. Results: A positive pregnancy test was defined as ultrasonographic evidence indicating presence of a fetal sac 6 weeks after embryo transfer. Of the 5 post-myomectomy women who were pregnant, only 1 (20.0%) received 1-3 adjunct acupuncture sessions whilst the remaining 4 (80.0%) received > 3 acupuncture sessions. Of the 11 normal pregnant women, 5 (45.4%) received 1-3 adjunct acupuncture sessions while 6 (54.5%) received > 3 adjunct acupuncture sessions. Conclusion: Pregnancy rates in infertile post-myomectomy women may be improved by > 3 adjunct acupuncture sessions.

Induction of apoptosis in activated RAW 264.7 cells and inhibition of pro-inflammatory mediators in rat air pouch by ethylacetate fraction of Ocimum gratissimum leaves

Ajayi Abayomi Mayowa,Ben-Azu Benneth,Sikiru O. Balogun,Ruberlei Godinho de Oliveira,Umukoro Solomon,Domingos Tabajara de Oliveira,Olusegun G. Ademowo 경희대학교 융합한의과학연구소 2022 Oriental Pharmacy and Experimental Medicine Vol.22 No.3

Ocimum gratissimum L. has attracted substantial consideration from researchers because of its anti-inflammatory uses in ethnomedicine in Sub-Saharan Africa and Asia. This study investigated the effect of flavonoid-rich ethylacetate fraction of O. gratissimum (EAFOg) in apoptosis induction of activated macrophages and inflammatory response in LPS-induced air pouch in rats. Apoptotic effect of EAFOg in LPS-stimulated RAW 264.7 cells was evaluated using flow cytometry after staining with annexin-V and 7-aminoactinomycin D. Its effects on inflammatory cells and mediators were investigated utilizing 6 day old subcutaneous air pouch-rats. Sterile saline (0.9%) or LPS (100 ng/mL) was injected into the air pouch on 6th day after EAFOg (25, 50 and 100 mg/kg) pretreatment. Rectal body temperature was recorded hourly for 5 h after LPS injection. Thereafter, the neutrophil count, nitrite, TNF-α, PGE2, nitrite, malondialdehyde and reduced glutathione (GSH) levels were determined in the pouch lavage. The activities of myeloperoxidase and superoxide dismutase (SOD) as well as immunohistochemical staining for cyclooxygenase-2 were also performed. EAFOg (10, 30 and 100 μg/mL) induced apoptosis in LPS-stimulated RAW 264.7 macrophages. The EAFOg reduced hyperthermia and decreased neutrophil counts, TNF-α, PGE2, nitrite, myeloperoxidase as well as cyclooxygenase-2 expression evoked by LPS in rats. It also reduced malondialdehyde, and increased GSH and SOD levels in LPS-induced air pouch in vivo. The results of this study suggest that the EAFOg

Effect of hydrodymamic force and prolonged oxygen exposure on the performance of anodic biofilm in microbial electrolysis cells

Ajayi, F.F.,Kim, K.Y.,Chae, K.J.,Choi, M.J.,Kim, I.S. Pergamon Press ; Elsevier Science Ltd 2010 International journal of hydrogen energy Vol.35 No.8

In this work, the significance of Reynolds number (N<SUB>Re</SUB> in evaluating the performance of hydrogen production in MECs was demonstrated. Experiments performed with the same anode under the same operating conditions (applied potential, pH, stirring speed and substrate concentration) showed different performances when operated with different stirrers. An average increase of 30% in hydrogen production was obtained by increasing the diameter of the stirring bar from 1.2 cm to 2.8 cm. This increased the N<SUB>Re</SUB>from ∼900 to ∼4900. The anodic bacteria communities on MEC's anodes were also shown to be unaffected when exposed to oxygen for a prolonged period of time outside the reactor by storing them in buffer solution. This however was not enough to get rid of methanogenic bacteria which were still active on the electrodes after exposing them to oxygen in the air for 24 h and in buffer solution for 5 days.

Optimization studies of bio-hydrogen production in a coupled microbial electrolysis–dye sensitized solar cell system

Ajayi, Folusho Francis,Kim, Kyoung-Yeol,Chae, Kyu-Jung,Choi, Mi-Jin,Chang, In Seop,Kim, In S. Royal Society of Chemistry 2010 PHOTOCHEMICAL AND PHOTOBIOLOGICAL SCIENCES Vol.9 No.3

<P>Bio-hydrogen production in light-assisted microbial electrolysis cell (MEC) with a dye sensitized solar cell (DSSC) was optimized by connecting multiple MECs to a single dye (N719) sensitized solar cell (<I>V</I><SUB>OC</SUB> approx. 0.7 V). Hydrogen production occurred simultaneously in all the connected MECs when the solar cell was irradiated with light. The amount of hydrogen produced in each MEC depends on the activity of the microbial catalyst on their anode. Substrate (acetate) to hydrogen conversion efficiencies ranging from 42% to 65% were obtained from the reactors during the experiment. A moderate light intensity of 430 W m<SUP>−2</SUP> was sufficient for hydrogen production in the coupled MEC-DSSC. A higher light intensity of 915 W m<SUP>−2</SUP>, as well as an increase in substrate concentration, did not show any improvement in the current density due to limitation caused by the rate of microbial oxidation on the anode. A significant reduction in the surface area of the connected DSSC only showed a slight effect on current density in the coupled MEC-DSSC system when irradiated with light.</P> <P>Graphic Abstract</P><P>Hydrogen production in a coupled microbial electrolysis–dye sensitized solar cell system using a combination of bio- and photo-catalysis for a practical and efficient conversion of biomass to hydrogen at moderate light intensity. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=b9pp00097f'> </P>

Potentials of Kerstingiella geocarpa as a Health Food

Ajayi, O.B.,Oyetayo, F.L. The Korean Society of Food Science and Nutrition 2009 Journal of medicinal food Vol.12 No.1

The seed of Kerstingiella geocarpa was analyzed to determine its proximate and amino acid compositions. The seed was found to be a high source of crude protein (21.3%) and very low in crude fat (1.0%). Arginine, an amino acid for pediatric growth, was the most concentrated amino acid in the seed. Arginine (9.3 g/100 g of crude protein), histidine (2.1 g/100 g of crude protein), and phenylalanine (3.2 g/100 g of crude protein) concentrations in the seed were found in higher levels than the recommended Food and Agriculture Organization/World Health Organization amino acid requirement pattern for the preschool age group. The total essential amino acid content in K. geocarpa seed was 42.0%. The lysine:arginine ratio calculated to estimate the seed atherogenic potential was 0.3. This shows that K. geocarpa seed protein will not exert a hypercholesterolemia effect on the consumer. Furthermore, incorporation of the seed into diets of experimental rats resulted in hematological parameters comparable with those of casein control-fed rats. These findings show this seed could be an important source of food that holds promise in medicinal nutrition.

Serotype Distribution and Virulence Profile of Salmonella enterica Serovars Isolated from Food Animals and Humans in Lagos Nigeria

Ajayi Abraham,Smith Stella,Bode-Sojobi Ibidunni,Kalpy Julien Coulibaly,Jolaiya Tolulope Funbi,Adeleye Adeyemi Isaac 한국미생물·생명공학회 2019 한국미생물·생명공학회지 Vol.47 No.2

Distribution of Salmonella enterica serovars and their associated virulence determinants is wide-spread among food animals, which are continuously implicated in periodic salmonellosis outbreaks globally. The aim of this study was to determine and evaluate the diversity of five Salmonella serovar virulence genes (invA, pefA, cdtB, spvC and iroN) isolated from food animals and humans. Using standard microbiological techniques, Salmonella spp. were isolated from the feces of humans and three major food animals. Virulence determinants of the isolates were assayed using PCR. Clonal relatedness of the dominant serovar was determined via pulsed-field gel electrophoresis (PFGE) using the restriction enzyme, Xbal. Seventy one Salmonella spp. were isolated and serotyped into 44 serovars. Non-typhoidal Salmonella (NTS; 68) accounted for majority (95.8%) of the Salmonella serovars. Isolates from chicken (34) accounted for 47.9% of all isolates, out of which S. Budapest (14) was predominant (34.8%). However, the dominant S. Budapest serovars showed no genetic relatedness. The invA gene located on SPI-1 was detected in all isolates. Furthermore, 94% of the isolates from sheep harbored the spvC genes. The iroN gene was present in 50%, 100%, 88%, and 91% of isolates from human, chicken, sheep, and cattle, respectively. The pefA gene was detected in 18 isolates from chicken and a single isolate from sheep. Notably, having diverse Salmonella serovars containing plasmid encoded virulence genes circulating the food chain is of public health significance; hence, surveillance is required.

Study of hydrogen production in light assisted microbial electrolysis cell operated with dye sensitized solar cell

Ajayi, F.F.,Kim, K.Y.,Chae, K.J.,Choi, M.J.,Kim, S.Y.,Chang, I.S.,Kim, I.S. Pergamon Press ; Elsevier Science Ltd 2009 International journal of hydrogen energy Vol.34 No.23

Hydrogen production with light as an additional energy source in a microbial electrolysis cell (MEC) is described. A ruthenium-dye (N719) sensitized solar cell with an open circuit potential (V<SUB>oc</SUB>) of 602 mV was connected to the MEC. Hydrogen production was carried out by irradiating the DSSC connected across the MEC with a light intensity of 40 mW/cm<SUP>2</SUP> and also with natural sunlight. The DSSC was stable during various batch experiments. The acetate conversion efficiency and the coulombic efficiency based on the average of first two batches were 30.5 +/- 2.5% and 40 +/- 2% respectively. The cathodic recovery efficiency ranged from 72% to 86% during repeated batch experiments with an average of 78 +/- 2.5%.

Estimates of genetic parameters and correlation of morphological and physiological responses among accessions of cowpea screened under drought stress at the vegetative stage

Ajayi Abiola Toyin,Gbadamosi Alaba Emmanuel,Olumekun Victor Olugbenga,Adedeji Iyanu,Ibrahim Nofisat Ronke 한국작물학회 2023 Journal of crop science and biotechnology Vol.26 No.2

Drought is one of the major limiting factors to cowpea growth and productivity. However, improvement for drought tolerance requires adequate knowledge of the level of genetic variability for diferent morphological and physiological traits, their interactions, and how they contribute to yield under drought stress. The present study assessed the level of genetic variation in morphological and physiological responses of selected accessions of cowpea under drought stress at the vegetative stage. The experiment was conducted in the screen house in pots flled with topsoil; when plants were 5 weeks old, drought stress was imposed by withholding watering for three weeks. Signifcant diferences were observed among accessions for measured parameters. The most susceptible accession (ACC10) had the highest percentage of wilted plants (PWP, 96.67%), the lowest percentage recovery (8.86%), and seed yield (0.00 g). Mean tolerance indices ranged from 0.72 in accession ACC10 to 1.27 in accession ACC07. ACC07 and accession ACC09 were the highly tolerant accessions, while ACC10 and accession ACC04 were the highly susceptible ones. Parameters such as chlorophyll B and total chlorophyll were reduced signifcantly in all accessions under drought stress, but chlorophyll A and stomatal parameters increased in very few accessions. High heritability (≥60%) and high genetic advance as a percent of the mean (≥20%) displayed among traits such as stomatal parameters, PWP, and seed yield under drought stress suggest that such traits are reliable parameters for efective selection of drought-tolerant cowpea genotypes for regions aficted with moisture defcit. Principal Component Analysis (PCA) also showed that stomatal parameters had higher contributions to the variability of the crop under drought stress compared to chlorophyll parameters in most cases, making it more preferred parameters for selection to be efective.

Antioedematogenic and anti-inflammatory actions of Phragmanthera incana (Schum) Balle leaf in carrageenan-induced inflammation models in rats

Ajayi Abayomi Mayowa,Alabi Akinyinka O.,Oyibo Alice O.,Joseph Olushola O. 경희대학교 융합한의과학연구소 2021 Oriental Pharmacy and Experimental Medicine Vol.21 No.4

Phragmanthera incana (Schum) is a specie of mistletoe that has been mentioned in Nigeria ethnomedicine to be used in the treatment of inflammatory disorders. The aim of this study was to investigate the anti-inflammatory effect of Phragmanthera incana leaf ethanol extract (PIEE) in carrageenan-induced inflammation models in rats. In the carrageenan-induced rat paw oedema experiment, 0.1 mL of carrageenan (1%) was injected into the paw one hour after treatment with PIEE (125, 250 and 500 mg/kg). Paw volume was measured hourly for 4 h using the plethysmometer. The anti-inflammatory activity was investigated using the carrageenan-induced air pouch inflammation model. Leucocytes migration and pro-inflammatory mediators (TNF-α and IL-6) were evaluated in the air pouch exudate. Oxidative stress biomarkers (MDA, Nitrite, GSH and Catalase) were estimated in both the carrageenan-induced paw tissue homogenates and air pouch exudate. PIEE (125, 250 and 500 mg/kg) administration significantly (p < 0.05) reduced paw oedema hourly post-carrageenan injection. PIEE administration displayed dose-dependent significant (p < 0.05) reduction in fluid exudates and total leucocytes counts respectively. The extract administration also decreased elevated pro-inflammatory cytokines (TNF-α and IL-6) levels respectively. PIEE administration demonstrated antioxidant activity by attenuating carrageenan-induced increase in malondialdehyde and nitrite as well as decrease in reduced glutathione and catalase levels in rats. Phragmanthera incana leaf ethanol extract possess anti-inflammatory properties, suggesting protective function in inflammatory diseases.