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Chen, Long,Li, Yanhui,Chen, Lina,Li, Na,Dong, Chenglong,Chen, Qiong,Liu, Beibei,Ai, Qing,Si, Pengchao,Feng, Jinkui,Zhang, Lin,Suhr, Jonghwan,Lou, Jun,Ci, Lijie Elsevier 2018 CHEMICAL ENGINEERING JOURNAL -LAUSANNE- Vol.345 No.-
<P><B>Abstract</B></P> <P>A flexible and free-standing graphene oxide and nylon 6 (GO@nylon 6) multilayer nanofiltration membrane was prepared by a layer-by-layer assembly process. The combination of electrospinning and electrospraying technique was employed, which can facilely prepare large-area membrane with size of 20 × 30 cm. The mechanical stability of multilayer membrane has enhanced significantly due to the tightly locked structure achieved by nylon 6 nanofibers network. The novel GO@nylon 6–13 multilayer nanofiltration membrane demonstrated a pure water flux up to 11.15 L m<SUP>−2</SUP> h<SUP>−1</SUP> bar<SUP>−1</SUP>, while keeping high organic dye rejection rate (>95% for methylene blue, and >99% for methyl orange). The rejections rate of the Na<SUB>2</SUB>SO<SUB>4</SUB>, NaCl, CuSO<SUB>4</SUB>, and Pb(NO<SUB>3</SUB>)<SUB>2</SUB> were 56.5%, 27.6%, 36.7%, and 18.9%, respectively. Furthermore, GO@nylon 6–13 multilayer nanofiltration membrane also demonstrated a high flux of some common organic solvents (8.4, 5.3, and 0.8 L m<SUP>−2</SUP> h<SUP>−1</SUP> bar<SUP>−1</SUP> for methanol, ethanol, and NMP, respectively), showing excellent chemical stability for separation process in those solvents.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Large-area GO@nylon 6 multilayer nanofiltration membrane was prepared. </LI> <LI> The multilayer structure enhances the mechanical stability. </LI> <LI> The multilayer membrane demonstrates a high water flux. </LI> <LI> The multilayer membrane shows high rejection rate for organic dyes. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>Photograph and cross-section SEM image of GO@nylon 6 multilayer nanofiltration membrane, the inset shows the water contact angle.</P> <P>[DISPLAY OMISSION]</P>
( Ai Hua Deng ),( Guo Qiang Zhang ),( Na Na Shi ),( Jie Wu ),( Fu Ping Lu ),( Ting Yi Wen ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.2
A novel protease gene from Bacillus gibsonii, aprBG, was cloned, expressed in B. subtilis, and characterized. High-level expression of aprBG was achieved in the recombinant strain when a junction was present between the promoter and the target gene. The purified recombinant enzyme exhibited similar N-terminal sequences and catalytic properties to the native enzyme, including high affinity and hydrolytic efficiency toward various substrates and a superior performance when exposed to various metal ions, surfactants, oxidants, and commercial detergents. AprBG was remarkably stable in 50% organic solvents and retained 100% activity and stability in 0-4 M NaCl, which is better than the characteristics of previously reported proteases. AprBG was most closely related to the high-alkaline proteases of the subtilisin family with a 57-68% identity. The secretion and maturation mechanism of AprBG was dependent on the enzyme activity, as analyzed by site-directed mutagenesis. Thus, when taken together, the results revealed that the halo-solvent-tolerant protease AprBG displays significant activity and stability under various extreme conditions, indicating its potential for use in many biotechnology applications.
Liu, Ai-Na,Sun, Ping,Liu, Jian-Nan,Ma, Jin-Bo,Qu, Hua-Jun,Zhu, Hua,Yu, Cai-Yan,Zhang, Liang-Ming Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.4
Objective: To study the relationship between clinical pathologic characteristics, treatment modalities and prognostic factors in HER-2 (Human Epidermal growth factor Receptor-2) overexpressed breast carcinoma. Materials and Methods: Major clinico-pathological factors including therapeutic modalities and survival status of 371 breast cancer patients with HER2 over-expression, teated at Yantai Yuhuangding Hospital from March of 2002 to December of 2010 were retrospectively studied, with special attention focused on survival-related factors. Results: The median age of the total 371 patients in this study was 48 years at time of diagnosis, among which, the leading pathological type was infiltrating ductal carcinoma (92.5%); 62.8% presented with a primary tomor larger than 2 cm in diameter at diagnosis, 51.0% had axillary lymph node (ALN) metastases; ER (Estrogen receptor)/PR (Progesterone receptor) double negative occured in 52.8% of cases, and PCNA (proliferation cell nuclear antigen) (+++) was found in 55.1%. HER-2 overexpressed patients were usually in advanced stage when the diagnosis was made (72.8% at stages IIA~IIIC). The prognosis and survival were assessed in 259 patients with complete follow-up data. 5-year DFS (disease-free survival) and OS (overall survival) rate was 68.0% and 78.0% respectively. Univariate analysis revealed that age, tumor size, ALN metastases, LVSI (lymph-vascular space involvement), PCNA status, hormonal therapy, chemotherapy cycles, and HER-2 overexpression, correlated closely with the prognosis. ALN metastases, LVSI, PCNA status and chemotherapy cycles were independent predictors of survival. Conclusions: HER-2 overexpressed breast cancer has special clinical and pathological characteristics, with advanced clinical stages and high rate of ER/PR double negative. Lymph node metastases, LVSI, PCNA and chemotherapy cycles are independent predictors of prognosis.
Ai, T.N.,Naing, A.H.,Arun, M.,Lim, S.H.,Kim, C.K. Elsevier Scientific Publishers Ireland Ltd 2016 Plant science Vol.252 No.-
<P>The effects of three different sucrose concentrations on plant growth and anthocyanin accumulation were examined in non-transgenic (NT) and transgenic (T-2) specimens of the Petunia hybrida cultivar 'Mirage rose' that carried the anthocyanin regulatory transcription factors B-Peru + mPAP1 or RsMYB1. Anthocyanin accumulation was not observed in NT plants in any treatments, whereas a range of anthocyanin accumulation was observed in transgenic plants. The anthocyanin content detected in transgenic plants expressing the anthocyanin regulatory transcription factors (B-Peru + mPAP1 or RsMYB1) was higher than that in NT plants. In addition, increasing sucrose concentration strongly enhanced anthocyanin content as shown by quantitative real-time polymerase chain reaction (qRT-PCR) analysis, wherein increased concentrations of sucrose enhanced transcript levels of the transcription factors that are responsible for the induction of biosynthetic genes involved in anthocyanin synthesis; this pattern was not observed in NT plants. In addition, sucrose affected plant growth, although the effects were different between NT and transgenic plants. Taken together, the application of sucrose could enhance anthocyanin production in vegetative tissue of transgenic Petunia carrying anthocyanin regulatory transcription factors, and this study provides insights about interactive effects of sucrose and transcription factors in anthocyanin biosynthesis in the transgenic plant. (C) 2016 Elsevier Ireland Ltd. All rights reserved.</P>
Ai, Trinh Ngoc,Naing, Aung Htay,Arun, Muthukrishnan,Jeon, Su Min,Kim, Chang Kil International Society for Horticultural Science 2017 Scientia horticulturae Vol. No.
<P><B>Abstract</B></P> <P>Anthocyanin production enhanced by heterologous expression of R2R3 MYB transcription factors has been studied. However, little is known about validated information on the ability of <I>RsMYB</I> (an MYB gene from radish) to enhance anthocyanin production in a heterologous system. In the present study, heterologous expression of <I>RsMYB1</I> in <I>Petunia hybrida</I> ‘Mirage Rose’ enhanced anthocyanin production in vegetative and floral tissues such as leaves, stems, roots, and petals by transcriptional activation of anthocyanin biosynthetic genes and endogenous antocyanin regulatory genes. Line PM6 expressed higher transcript levels of <I>RsMYB1</I> than line PM2 and regulated transcript levels of the investigated genes largely than line PM2, whereas those regulated in wild type (WT) plants were the lowest. In addition, transcript levels of the genes detected using qualitative real-time polymerase chain reaction were found to be higher in petals, followed by leaves, stems and roots. Taken together, our results suggest that <I>RsMYB1</I> enhances anthocyanin production in vegetative and floral tissues of this cultivar, thus, we expect that heterologous expression of <I>RsMYB1</I> would help to modify flower color of other ornamental plants as well.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Expression of <I>RsMYB1</I> in <I>Petunia</I> enhanced anthocyanin production in vegetative and floral tissues. </LI> <LI> RsMYB1 enhances transcript levels of several anthocyanin biosynthetic genes and endogenous genes. </LI> <LI> Transcript levels of <I>RsMYB1</I> and investigated genes were found to be higher in leaves, flowers, followed by stems and roots. </LI> </UL> </P>
Ai, Trinh Ngoc,Naing, Aung Htay,Kim, Chang Kil The Korean Society of Plant Biotechnology 2016 식물생명공학회지 Vol.43 No.1
Anthocyanin accumulation and plant growth were examined in petunia (NT and $T_2$ transgenic plants) by determining the effects of different sources of light and varying light/dark cycles. Red light significantly enhanced anthocyanin content of B-peru+mPAP1; however, it had a negative effect on anthocyanin production in RsMYB1 plants. In general, white light was found to be reasonable for anthocyanin accumulation in all plants. In case of light/dark cycles, application of seven days of light:14 days of dark significantly enhanced anthocyanin content. We found that anthocyanin content detected in transgenic plants expressing anthocyanin regulatory transcription factor genes (B-peru+mPAP1 or RsMYB1) was higher than that in NT plants in all treatments. Plant growth was also influenced by the different light sources and dark/light cycles. Taken together, our results suggest that light source and light/dark cycle play an important role in anthocyanin production and plant growth. The choice of the optimal conditions is also important for anthocyanin production and plant growth depending on NT or transgenic plants carrying anthocyanin regulatory transcription factors.
Ai, Trinh Ngoc,Arun, Muthukrishnan,Naing, Aung Htay,Lim, Sun-Hyung,Kim, Chang Kil Elsevier 2016 Scientia horticulturae Vol.200 No.-
<P><B>Abstract</B></P> <P>The present study aimed to determine the role of transcription factors (bHLH and MYB) in enhancing anthocyanin production in <I>Petunia</I> ‘Mirage rose’. Initially, we optimized an <I>Agrobacterium</I>-mediated transformation protocol to over-express transcription factors (<I>B-Peru</I>, <I>mPAP1</I>, and <I>B-Peru</I> + <I>mPAP1</I>) in <I>Petunia.</I> Phosphinothricin (PPT) concentrations of 0.5, 1.0, and 1.5mgl<SUP>−1</SUP> were found to be ideal for the selection and regeneration of transformed shoots at different developmental stages. Clavamox (250mgl<SUP>−1</SUP>) efficiently eliminated <I>Agrobacterium</I> after co-cultivation (2 days) and favored maximum shoot regeneration. In addition, incubation for 30min in <I>Agrobacterium</I> suspension increased the number of transformed cells and resulted in improved regeneration in the selection medium. The transformed plants were successfully developed through a direct organogenesis system. The transformed plants were selected using BASTA<SUP>®</SUP> and the presence of transgenes was assessed using PCR. Visible anthocyanin accumulation was evident only in plantlets (shoots, stem, leaves, and roots) carrying the gene combination <I>B-Peru</I> <I>+</I> <I>mPAP1</I>. The expression of biosynthetic genes involved in the flavonoid pathway was analyzed using quantitative real-time PCR. Expression levels of <I>PAL</I>, <I>CHS</I>, <I>CHI</I>, <I>F3H</I>, <I>DFR</I>, and <I>ANS</I> were higher in the young and mature leaves of plants transformed with <I>B-Peru</I> <I>+</I> <I>mPAP1</I> compared to those transformed with <I>B-Peru</I>, <I>mPAP1,</I> and/or non-transformed plants. Furthermore, the highest level of anthocyanin was recorded in the leaves, stem, and roots of plants transformed with <I>B-Peru</I> <I>+</I> <I>mPAP1</I>. These results validate the combinatorial requirement of <I>B-Peru</I> and <I>mPAP1</I> to enhance anthocyanin content in <I>Petunia hybrida</I> ‘Mirage rose’.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We report an improved <I>Agrobacterium</I>-mediated transformation protocol for <I>Petunia</I>. </LI> <LI> PPT used throughout the selection procedure reduced the total number of escapes. </LI> <LI> Clavamox controlled <I>Agrobacterium</I> after co-cultivation and supported regeneration. </LI> <LI> The protocol was utilized to transfer anthocyanin regulatory genes into <I>Petunia</I>. </LI> <LI> Co-expression of <I>B-peru</I> and <I>mPAP1D</I> significantly improved anthocyanin in <I>Petunia</I>. </LI> </UL> </P>