체외 방사선조사시 인체 말초혈액 임파구의 염색체이상 빈도에 관한 연구

안용찬,하성환 대한방사선 방어학회 1993 방사선방어학회지 Vol.18 No.2

급성 전신 및 부분 피폭시 피해자들에 대한 치료방침의 결정에 있어 참고자료로서 사용하기 위하여 저자들은 체외에서 말초혈액을 60 Coγ-선으로 2Gy에서 12Gy까지 방사선 조사하여 말초 임파구에서 관찰되는 염색체이상의 빈도와 방사선량과의 관계를 실험적으로 연구하였다. 관찰된 세포중 불안정 염색체이상(dicentric 염색체, ring 염색체, acentric fragment쌍)이 나타난 세포의 비율은 2Gy에서 32%, 4Gy에서 47%, 6Gy에서 80%, 8Gy에서 94%, 10Gy이상에서는 100%였다. 급성 전신 피폭시 평균 흡수선량을 반영하는 지표인 Ydr은 2Gy에서 0.373, 4Gy에서 0.669, 6Gy에서 1.734, 8Gy에서 2.773, 10Gy애소 3.746 그리고 12Gy에서 5.454였다. 방사선량(D)과 염색체이상(dicentric 염색체와 ring 염색체의 합) 빈도(Ydr)와의 관계는 Ydr=9.322×10- ²/Gy×D+2.975×10-²/Gy²D²로 나타났다. 신체의 부분피폭시의 선량 및 과거의 피폭선량을 계산할 때 사용하는 지표인 Qdr은 2Gy에서 1.166, 4Gy에서 1.436, 6Gy에서 2.173, 8Gy에서 2.945, 10Gy에서 3.746, 그리고 12Gy에서 5.454였다. 이와 같은 선량측정방법의 신빙도를 검증하기 위하여 신체의 부분에 균일한 선량분포의 1.8Gy, 2.5Gy 및 7.0Gy의 방사선치료를 일회 받은 환자들로부터 구한 Qdr은 각각 1.109, 1.222, 2.222였으며 이로부터 Qdr=Ydr/(1-e -Ydr)의 관계식을 이용하여 계산해 낸 피폭선량은 1.52Gy, 2.48Gy 및 6.54Gy로서 실제 조사한 선량과 매우 근사한 결과를 얻었다. As guides to decision-making in the management of the victims in case of acute whole body or partial body radiation exposure, we studied the relationship between radiation dose and the frequency of chromosomal aberrations observed in peripheral lymphocytes that were irradiated in vitro with ??Co γ-rays at dodws ranging from 2Gy to 12Gy. The yields of cells with unstable chromosomal aberrations (dicentric chromosomes, ring chromosomes, and acentric fragment pairs) were 32% at 2Gy, 47% at 4Gy, 80% at 6Gy, 94% at 8Gy, and 100% at 10Gy and over. Ydr, which reflect average dose to the whole body in case of acute whole body exposure, were 1.373 at 2Gy, 0.669 at 4Gy, 1.734 at 6Gy, 2.773 at 8Gy, 3.746 at 10Gy and 5.454 at 12Gy. The relationship between radiation dose (D) and the friquency of dicentric plus ring chromosomes per cell(Ydr) could be expressed as Ydr=9.322×10-²/Gy×D+2.975×10-²/Gy²×D². Qdr, which are used in estimating dose of partial body exposure and dose of past exposure, were 1.166 at 2Gy, 1.436 at 4Gy, 2.173 at 6Gy, 2.945 at 8Gy, 3.746 at 10Gy and 5.454 at 12Gy. To see how confidently this dosimetry system may be used, we obtained Qdr values from those who received one fraction of homogenous partial body irradiation of 1.8Gy, 2.5Gy, and 7.0Gy therapeutically; in vivo Qdr values were 1.109, 1.222 and 2.222 rewpectively. The estimated doses calculated from these in vivo Qdr values using the equation Qdr=Ydr/(1-eYdr) were 1.52Gy, 2.48Gy, and 6.54Gy respectively, which were very close to the doses actually given.

A Comparison of Lyophilized Amniotic Membrane with Cryopreserved Amniotic Membrane for the Reconstruction of Rabbit Corneal Epithelium

Ahn Jae-Il,Jang In-Keun,Lee Doo-Hoon,Seo Young-Kwon,Yoon Hee-Hoon,Shin Youn-Ho,Kim Jae-Chan,Song Kye-Yong,Lee Hee-Gu,Yang Eun-Kyung,Kim Ki-Ho,Park Jung-Keung The Korean Society for Biotechnology and Bioengine 2005 Biotechnology and Bioprocess Engineering Vol.10 No.3

Many researchers have employed cryopreserved amniotic membrane (CAM) in the treatment of a severely damaged cornea, using corneal epithelial cells cultured on an amniotic membrane (AM). In this study, two Teflon rings were made for culturing the cells on the LAM and CAM, and were then used to support the AM, which is referred to in this paper as an Ahn's AM supporter. The primary corneal epithelial cells were obtained from the limbus, using an ex-plantation method. The corneal epithelium could be reconstructed by culturing the third­passage corneal epithelial cells on the AM. A lyophilized amniotic membrane (LAM) has a higher rate of graft take, a longer shelf life, is easier to store, and safer, due to gamma irradiation, than a (AM. The corneal epithelium reconstructed on the LAM and (AM, supported by the two­Teflon rings, was similar to normal corneal epithelium. However, the advantages of the LAM over that of the (AM make the former more useful. The reconstruction model of the corneal epithelium, using AM, is considered as a good in vitro model for transplantation of cornel epithelium into patients with a severely damaged cornea.

Reconstruction of Rabbit Corneal Epithelium on Lyophilized Amniotic Membrane Using the Tilting Dynamic Culture Method

Ahn, Jae-Il,Lee, Doo-Hoon,Ryu, Yang-Hwan,Jang, In-Keun,Yoon, Mun-Young,Shin, Youn Ho,Seo, Young-Kwon,Yoon, Hee-Hoon,Kim, Jae-Chan,Song, Kye-Yong,Yang, Eun-Kyung,Kim, Ki-Ho,Park, Jung-Keug Blackwell Publishing Inc 2007 Artificial Organs Vol.31 No.9

<P>Abstract: </P><P>Rabbit corneal epithelium was reconstructed using tilting dynamic culture with a self-manufactured, amniotic membrane (AM) supporter and a lyophilized amniotic membrane (LAM). Rabbit corneal epithelial (RCE) cells were cultured and cryopreserved after isolation from the limbus. The second- and third-passage RCE cells were plated onto the epithelial side of the LAM of Ahn's AM supporter. Two days later, the air–liquid interface culture was maintained with third-passage RCE cells for 6 days and second-passage corneal epithelial cells for 9 days. The average viability of thawed RCE cells, assessed using trypan blue dye exclusion, was 77.42%. The reconstructed corneal epithelium was characterized by histological (hematoxylin and eosin) and immunohistochemical staining (proliferating cell nuclear antigen) for light microscopy, and by reverse transcriptase-polymerase chain reaction, glucose assay, and transmission electron microscopy. The basal layer of the reconstructed corneal epithelium was well formed, and the epithelium was tightly constructed due to the increase in cell proliferation and differentiation caused by the tilting dynamic culture, as opposed to static culture. Tilting dynamic culture was useful for the reconstruction of the epithelium using easily damaged epithelial cells and resulted in more stratum cell layers. Moreover, cytokeratin (CK3) mRNA expression in tilting dynamic cultured third-passage RCE cells seeded onto AM was greater than in static cultured third-passage RCE cells. The morphology of the reconstructed corneal epithelium on LAM by tilting dynamic culture for 9 days resembled that of the skin epidermis. This was thought to be because the tilting dynamic culture not only accelerated the proliferation and differentiation of cells by physical or mechanical stimulation, but also ensured that the supply of medium was delivered to the basal cells more efficiently. Thus, the reconstruction of the corneal epithelium using LAM and tilting dynamic culture was considered to be a good in vitro model for autologous or allogeneic transplantation of corneal epithelium and skin epidermis in patients with damaged epithelia. </P>

선속 변화에 의한 능동형 횡 동요감쇠장치의 효과

최찬문,안장영,서두옥,정용진 濟州大學校 海洋硏究所 2004 해양과환경연구소 연구논문집 Vol.28 No.1

제주대학교 실습선 아라호에 장착된 능동형 횡 동요 감쇠장치의 성능이 선박의 속력과의 관계를 파악 하기 위하여 선박을 정지, 미속, 중속, 전속으로 구분하여 횡 동요 감쇠장치를 정지(Passive A.R.T), 작동(Active A.R.T)을 했을 때 경사계에 의한 횡 동요각 및 종 동요각, 풍속계에 의한 풍속의 변화에 의한 분석한 결과를 요약하면 다음과 같다. 1. 선박이 정지했을 때가 능동형 횡 동요 감쇠장치의 1/3, 1/10 횡 동요각 유의 진폭에서 감소률이 가장 높아 47.2%, 50.3%를 나타냈으며, 반면에 중속인 경우 가장 낮은7.1%, 7.4% 나타내므로, 이 장치는 선속의 변화에 차이가 있는 것으로 판단된다. 2. 선속의 변화에 따라 그 효율이 많은 차이가 있으므로 이 장치를 장착한 선박의 운항자는 이를 감안하여 작동하여야 할 것으로 생각되며, 통계분석 결과에 의해서 선속과 감소률의 관계를 회귀분석식으로 나타내어 보면 다음과 같았다. 즉, 횡동요각 유의 진폭 (Amplitude of and significant roll (φ_(1/3)) : y= -3.233χ + 45.613 횡동요각 유의 진폭 (Amplitude of and significant roll (φ_(1/10)) : y= -3.157χ + 46.840을 각각 얻을 수 있었다. This experimental paper deals with the performance of tanks that are turned the active A.R.T(Anti-Rolling Tank) when the fluid transfers from wing tanks to the opposite tank by the power developed by the automatic control system (INTERING Stabilizer), which was installed in the fishery training ship T/S. A - RA (G/T : 990 tons) of Cheju National University. In this paper, the author has tested the performance of INTERING Stabilizer from the signals obtained by the inclinometer in irregular waves and compared with the results obtained in passive mode operation at stop and at various ship speeds. The performances of the system were confirmed the results as follows through the tests : 1. The Rates of reduction in significant roll(φ_(1/3)) of performance of Active-Rolling Tank at stop. dead slow ahead, half ahead, full ahead were obtained 47.2%, 26.3%, 7.7% 9.7%, respectively. 2. The operators had to consider to be different to change according to the various ship speeds for working. and the author could get the linea regressions about the rates of reduction at the results of a statistical analysis through the time histories of rolling angles in A.R.T. in irregular waves using various ship's speeds. Rates of reduction in significant roll(φ_(1/3)) : y = -3.233χ + 45.613 Rates of reduction in significant roll(φ_(1/10)) : y = -3.157χ + 46.840

고요산혈증의 위험인자 : 아산시 지역 주민을 대상으로

김용배,장원기,황보영,김화성,안규동,이병국,이용진,이종은,이준숙,이찬구,이한정,이성수 순천향대학교 2006 Journal of Soonchunhyang Medical Science Vol.12 No.1

Objective : Serum uric acid has now been identified as a marker for a number of metabolic and hemodynamic abnormalities. The aim of this study is to evaluate risk factors which influence hyperuricemia in the citizens of Asan. Method : The study design was cross-sectional and research subjects were 1086 citizens of Asan. General characteristics, cigarette smoking, alcohol consumption, and past history were obtained by interview using a structural questionnaire. Also, we measured height, weight and blood pressure, and serum uric acid of the subjects. Results : The mean serum uric acid level was 5.27 ㎎/㎗ in men and 4.01 ㎎/㎗, in women, In the multiple logistic regression analysis, statically significant risk factors of hyperuhcemia were gender, body mass index, blood lead concentration, and serum creatinine. Conclusion : Our results suggest that gender, body mass index, blood lead concentration, and serum creatinine might be the most important risk factors of hyperurcemia. Education program as well as routine check-up for serum uric acid was highly recommended for the effective prevention of hyperuricemia.

김치유래 젖산균의 균체지방산 분석을 이용한 분류학적 연구

이정숙,정민철,김우식,이근철,김홍중,박찬선,이헌주,주윤정,이근종,안종석,박완,박용하,민태익 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.2

표준균주를 포함한 230여개의 김치유래 젖산균에 대한 균체지방산(FAMEs)을 분석하였다. FAMEs profiles는 Euclidian Distance 17.5에 의해 7개의 Major Cluster와 1개의 Single Cluster로 나뉘어졌다. 이중 A, B, C 및 Cluster는 Leuconostoc속으로 분석되어졌고, F는 Lactobacillus속으로 분석되어졌다. 그리고 E와 G cluster는 두개의 Genus가 혼재되어 나타났으며 보충적인 연구가 필요하다. 앞으로 김치유래 젖산균의 균체지방산 분석결과를 기반으로 한 데이타베이스에 95가지 탄소원을 이용하는 수치분류학적 접근방법 및 Pyrolysis Mass Spectrometry 등의 화학적 분석 방법과 분자친화적 연구를 통한 종합적 분류정보 체계가 갖추어지면 젖산균의 신속, 정확한 동정 및 연구에 활발히 이용되어질 것이다. Two hundreds and thirty lactic acid bacteria, mostly isolated from Kimchi, including type strains were sued for analysis of cellular fatty acids. The 230 test strains were recoverd in 7 major and 1 single clusters defined a Euclidian distance of 17.5. These aggregate taxa were equivalent to the genus Leuconostoc (aggregate group A, B, C and D), and the genera Leuconostoc and Lactobacillus (aggregate group G). It is concluded as evident that FAMEs (Fatty Acid Methyl Esters) profile of cell can be used as a criterion in classification of lactic acid bacterial from kimchi. Additional comparative taxonomic studies need to be carried out on well chosen representative strains to determine the most appropriate methods of value.

잉어 담낭 생식 후 병발된 횡문근융해증 및 급성신부전과 급성간염 1예

여동근,박정기,윤원찬,정의달,김학준,조선주,이인희,안기성,김용진 대한신장학회 2000 대한신장학회잡지 Vol.19 No.5

발포 알루미늄 샌드위치 패널의 차음성능

안용찬(Ahn Yong-Chan),이중혁(Ahn Yong-Chan),변준호(Byeon Jun-Ho),김석현(Kim Seock-Hyun) 강원대학교 산업기술연구소 2017 産業技術硏究 Vol.37 No.1

Speeding up of railway vehicles requires weight reduction of the vehicle body. However, when the vehicle body is lighter, the sound insulation performance for blocking the noise from the outside is reduced. Aluminum is an important material used in the bodywork of transportation vehicles such as railway vehicles, aircraft, and automobiles. In this study, the bending stiffness and sound insulation performance of foamed aluminum with sandwich structure are investigated experimentally. The transmission loss is measured in accordance with the international standard ASTM E 2249-02. The mass-law deviation is used to evaluate the sound insulation performance per weight. In order to examine the applicability of the foamed aluminum sandwich panel to railway vehicles, the analysis of bending stiffness and an experimental review are carried out at the same time.

Less is more: role of additional chemotherapy to concurrent chemoradiotherapy in locoregionally advanced nasopharyngeal cancer management

Yong Chan Ahn 대한방사선종양학회 2019 Radiation Oncology Journal Vol.37 No.2

Concurrent chemoradiation therapy (CCRT) has played the most important and central role in the definitive therapy for the patients with locoregionally advanced stage nasopharynx cancer. The addition of induction chemotherapy (IC) or adjuvant chemotherapy (AC) to CCRT have been widely accepted with the rationale of improving distant control in the clinical practices. This review article investigated the role of IC and AC based on 11 recent meta-analysis publications, and found that the clinical benefits obtained by the additional IC or AC to CCRT, at the cost of the increased risks of more frequent and more severe side effects, seemed not big enough. More intervention is not always better, however, less seems frequently good enough. The author would speculate that ‘less is more’ and would advocate CCRT alone as the current standard.

My view-point as Editor-in-Chief of Cancer Research and Treatment

Yong Chan Ahn 대한종양외과학회 2022 대한임상종양학회 학술대회지 Vol.2022 No.2