GO-50 : Correlation between location of transposed ovary and function in patients with cervical cancer who underwent radical hysterectomy

( Aera Yoon ),( Yoo Young Lee ),( Jin Young Park ),( Tae Joong Kim ),( Chel Hun Choi ),( Jeong Won Lee ),( Byoung Gie Kim ),( Duk Soo Bae ) 대한산부인과학회 2012 대한산부인과학회 학술대회 Vol.99 No.-

To investigate the association between the location of transposed ovaries and ovarian function in patients with early cervical cancer (IB1-IIA). A retrospective study was performed with cervical cancer patients who underwent radical hysterectomy with ovarian transposition at Samsung Medical Center between July 1995 and July 2012. Ovarian function was estimated using serum FSH levels. We identified 31 patients and 21 patients were enrolled in this study. The median age and body mass index (BMI) were 31 years (range, 24 - 39) and 21.3 kg/m2 (range, 17.7 - 31.2). The median serum FSH level was 7.9 mlU/ml (range, 2.4 - 143.4). The median location of transposed ovary based on iliac crest was 0.5 cm (range, -2.7 - 5.2). As the location of transposed ovary heightened, serum FSH and E2 showed negative (r = -0.151, p =0.514) and positive correlation (r = 0.337, p=0.135). In the multivariate analysis, transposed ovarian function, which was assessed by serum FSH levels, was significantly associated with location of transposed ovary (β = -8.1, p=0.032), concurrent chemoradiotherapy (CCRT) (β = 71.08, p=0.006) and BMI (underweight; β = -59.93, p=0.05, overweight; β = -40.62, p=0.041). There was no significant association between transposed ovarian function and age. Location of transposed ovary, postoperative adjuvant CCRT and BMI are associated with ovarian function. We suggests that ovary should be transposed as highly as possible for preservation of the ovarian function.

GO-32 : Therapeutic Targeting of C-MET in Ovarian Clear-Cell Carcinoma

( Aera Yoon ),( Ji Yoon Ryu ),( Jung Joo Choi ),( Yoo Young Lee ),( Chel Hun Choi ),( Tae Joong Kim ),( Jeong Won Lee ),( Byoung Gie Kim ),( Duk Soo Bae ) 대한산부인과학회 2014 대한산부인과학회 학술대회 Vol.100 No.-

목적: Recent reports revealed that c-Met activation is specifically associated with epithelial ovarian carcinoma and is association with poor prognosis. In this study, we investigated c-Met expression and the effects of its therapeutic targeting in ovarian clear-cell carcinoma (CCC). 방법: Expression levels of c-Met in the CCCs, serous carcinoma and normal ovarian tissues were evaluated using real-time PCR. To test c-Met inhibitors in cell lines including ES2, RMG1, RMG2, OVISE and OVSAHO, we performed in vitro experiment including MTT and apoptosis assay. We performed Western blots to evaluate the c-Met expression and down-stream pathway. Moreover, we performed the in vivo therapy experiment in patient-derived xenograft models (PDX, Avatar) of ovarian CCC to confirm these effects. 결과: The c-Met expression was significantly increased in CCCs compared with serous carcinoma and normal ovarian tissues (p<0.05). When we treated c-Met inhibitor (SU11274, Crizotinib) to ovarian CCC cells, cell viability was significantly decreased and apoptosis was significantly increased. Western blot assay demonstrated that the protein expressions for c-Met signaling pathway were decreased by c-Met inhibitors. Moreover, we found that c-Met inhibitor (SU11274) had significant decreased tumor weight compared with control in a case of PDX (Avatar) models for ovarian CCC. 결론: These results revealed that c-Met inhibitor have significant anti-tumor effects in ovarian CCC, and suggested that c-Met may have a potentials of therapeutic target for ovarian CCCs.

[P4-09] Microrobiological and sensory characteristics of electron beam irradiated Myungran Jeotkal and its ingredients during storage at 4℃

Aera Jang,Xiande Liu,Binna Kim,Yoon-Ji Kim,Byung-Ho Ko,Cheorun Jo 한국식품영양과학회 2007 한국식품영양과학회 학술대회발표집 Vol.2007 No.10

Effect of Dihydroxybenzoic Acid Isomers on the Analysis of Polyethylene Glycols in MALDI-MS

Aera Lee,Hyo-Jik Yang,Yangsun Kim,Jeongkwon Kim 대한화학회 2009 Bulletin of the Korean Chemical Society Vol.30 No.5

The effects of different dihydroxybenzoic acid (DHB) isomers, when used as matrix materials in matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), were investigated in analyses of polyethylene glycol(PEG) polymers. PEG polymers ranging from 400 to 8,000 Da were prepared in different DHB isomer matrices using solvent-based and solvent-free methods. PEG samples were detected only in matrices of 2,3-DHB, 2,5-DHB,and 2,6-DHB while the most intense peaks were observed using 2,6-DHB in both solvent-free and solvent-based preparations.

Effects of Doneness on the Microbial, Nutritional, and Quality Properties of Pork Steak of Different Thicknesses

Aera Jang,Hye-Jin Kim,Dongwook Kim,JinSoo Kim,Sung-Ki Lee 한국축산식품학회 2019 한국축산식품학회지 Vol.39 No.5

This study aimed to evaluate the effect of doneness on the microbial, nutritional, and quality characteristics of 1.5 cm- and 2.0 cm-thick pork neck steaks. Pork neck meat was obtained within 24 h after slaughtering, cut into 1.5 cm- and 2.0 cm-thick slices (n=5), packed in LLD-PE wrap, and stored at 4±2℃ for 7–10 days until aerobic plate counts (APC) reach 5.51–6.50 Log CFU/g. Then, the pork meat was cooked on a frying pan till it was medium-rare, medium, or well-done. The microbial inhibition rates of the 1.5 cm- and 2.0 cm-thick steak in medium-rare state were 58.26% and 51.70%, respectively, whereas it was 100% for medium-done pork steak of either thickness. The total calories of the 1.5 cm- and 2.0 cm-thick well-done pork steaks were 643.61 kcal/100 g and 675.00 kcal/100 g, respectively, which was higher than that in medium-rare and medium-done steaks. The retention ratios for Fe and K in the well-done steak were significantly lower than those in the medium and medium-rare steak of either thickness (p<0.05). The shear force of the medium-rare and medium steak did not differ, whereas that of the well-done steak was significantly higher than that of the medium-rare steak of either thickness (p<0.05). We observed that the well-done pork steak had tough texture, low mineral content, and high calories. Therefore, consumption of medium and mediumrare pork is more beneficial than that of well-done pork.

Effect of Irradiation on the Quality of Pale, Soft, Exudative (PSE) Pork During Storage at 4℃

Aera Jang(장애라),Xiande Liu(류현덕),Cheorun Jo(조철훈) 충남대학교 농업과학연구소 2007 농업과학연구 Vol.34 No.1

방사선 조사가 PSE 돈육의 품질에 미치는 영향을 알아보기 위해 전문 육류등급사에 의해 외관적으로 확인된 PSE 돈육 등심을 4.5 kGy로 감마선 조사 후 pH, 육색, 지방산화, 미생물 및 관능적 품질을 2주간 냉장저장하면서 측정하였다. pH는 4.5 kGy의 방사선 조사에 의해 영향 받지 않았으나 육색 중 적색도를 나타내는 a<SUP>*</SUP>값은 방사선 조사된 등심근에서 저장기간 전체를 통하여 유의적으로 증가하였음을 관찰할 수 있었다(P<0.05). 방사선 조사 4.5 kGy는 비조사구(0 kGy)와 비교하여 2주간 냉장저장기간 중 약 2 log의 총균수 감소효과를 보였다. 관능검사 결과 적색도 증가에 따른 방사선 조사 돈육등심의 선호도가 높았다. 이상의 결과를 종합해 볼 때 식육 방사선 조사기술은 미생물학적 안전성뿐만 아니라 PSE 돈육의 육색을 향상시키는데 유용하게 사용될 수 있음을 알 수 있었다. The effect of irradiation on the quality characteristics of pale-soft-exudative (PSE) pork was studied. The pork loin with severe PSE appearance was selected by meat grader, irradiated at 0 and 4.5 kGy, and meat quality characteristics including pH, color, lipid oxidation development, microbial and sensory quality were analyzed during 2 weeks of storage at 4℃. The pH values of non-irradiated and 4.5 kGy-irradiated PSE raw pork loin was not different. Hunter color a<SUP>*</SUP>-value, which represents redness of meat surface showed significant increase (P<0.05) in irradiated PSE pork loin. This higher Hunter color a<SUP>*</SUP>-value maintained during 2 weeks of storage. By 4.5 kGy of irradiation, approximately 2 log reduction of total aerobic bacterial counts was achieved during 2 weeks of storage when compared with non-irradiated control. Sensory panelists prefer the color of irradiated PSE meat than that of non-irradiated control because of redness. From the results, it is possible to use an irradiation technology to improve not only microbiological safety but also the color of meat which has severe defect such as PSE.

GO-13 : Prognostic factors and outcomes in endometrial stromal sarcoma with the 2009 FIGO staging system; A multicenter review of 114 cases

( Aera Yoon ),( Jeong Yeol Park ),( Jin Young Park ),( Yoo Young Lee ),( Tae Joong Kim ),( Chel Hun Choi ),( Duk Soo Bae ),( Byoung Gie Kim ),( Jeong Won Lee ),( Joo Hyun Nam ) 대한산부인과학회 2012 대한산부인과학회 학술대회 Vol.99 No.-

To assess the prognostic factors associated with disease-related survival in endometrial stromal sarcoma (ESS), to which the 2009 FIGO staging system was applied. From January 1990 to January 2012, 114 patients with ESS were identified at the Samsung and Asan Medical Center and data were retrospectively analyzed. Ten (8.7%) patients died of the disease and 33 (28.9%) patients relapsed. The 5- and 10-year overall survival (OS) rates for the entire cohort was 92.6% and 87.1%, and the 5- and 10-year recurrence-free survival (RFS) rates were 71.8% and 52.1%. The estimated median survival after recurrence of 33 patients was 133 months (95% CI, 7.7 - 258.4), and 5-year survival after recurrence was 68.9%, retrospectively. Stage I (p=0.006), positive of estrogen and/or progesterone receptor (ER/PR) (p=0.0027) and no nodal metastasis (p=0.033) were associated with a good prognosis for OS in the univariate analysis. Ovarian preservation was revealed to be an independent predictor for poorer RFS (HR, 6.5; 95% CI, 1.23 - 34.19; p=0.027). Positivity of ER/PR (HR, 0.05; 95% CI, 0.006 - 0.4; p=0.006) and cytoreductive resection of recurred lesions (HR, 0.14; 95% CI, 0.02 - 0.93; p=0.042) were independent predictors for better survival after recurrence. Stage, expression of ER/PR and nodal metastasis are significantly associated with OS in ESS. Bilateral salpingo-oophorectomy (BSO) as the primary treatment and cytoreductive resection of recurred lesions should be considered for improving survival of patients with ESS.

Evaluation of New FIGO Staging System for Endometrial Stromal Sarcoma of the Uterus

( Aera Yoon ),( Jeong Yeol Park ),( Yoo Young Lee ),( Tae Joong Kim ),( Chel Hun Choi ),( Jeong Won Lee ),( Byoung Gie Kim ),( Duk Soo Bae ) 대한산부인과학회 2012 대한산부인과학회 학술대회 Vol.98 No.-

The aim of this study is to detect differences in overall survival between the 1988 FIGO staging and revised 2008 FIGO staging of uterine endometrial stromal sarcoma. The aim of this study is to detect differences in overall survival between the 1988 FIGO staging and revised 2008 FIGO staging of uterine endometrial stromal sarcoma. Of 109 patients with LGESS, 86 patients (78.9%) had stage I disease, 5 patients (4.6%) had stage II disease, 9 patients (8.3%) were diagnosed as stage III, and 9 patients (8.3%) had stage IV disease by 1988 FIGO staging system. According to revised 2008 FIGO staging system, 8 patients (7.3%) were downstaged; 3 patients were downstaged from stage II to I and 5 patients were downstaged from stage III to II respectively. The difference in median survival of 4 stages (I-IV) was statistically significant using both staging system (1998 vs. 2008 FIGO staging system, p=0.006 vs. p=0.013). Comparing the median survival of 2 subgroups, both staging system had significant difference but previous staging system had more accurate discriminability (1998 vs. 2008, p=0.006 vs. p=0.019, HR 5.470 vs.4.286). Based upon our analysis of 109 patients with uterine LGESS, the revised FIGO staging system was appropriate to predict survival.

Storage Stability of the Synthetic Angiotensin Converting Enzyme (ACE) Inhibitory Peptides Separated from Beef Sarcoplasmic Protein Extracts at Different pH, Temperature, and Gastric Digestion

Aera Jang,Cheorun Jo,Mooha Lee 한국식품과학회 2007 Food Science and Biotechnology Vol.16 No.4

The angiontensin converting enzyme (ACE) inhibitory peptides were separated from beef sarcoplasmic protein extract and their amino acid sequences were identified as GFHI, DFHINQ, FHG, and GLSDGEWQ. The 4 peptides were synthesized in a laboratory and the ACE inhibitory activities of peptides was measured after 2 months of storage at 4oC under different pH conditions (6.0, 6.5, 7.0, 7.5, and 8.0) and the exposure of different temperatures (70, 80, 90, and 100℃) for 20 min to evaluate industrial use. No significant difference was detected by pH and temperature abuse for 20 min during storage. When the synthetic peptides were digested by pepsin, trypsin, and chymotrypsin, the ACE inhibitory activity was not changed. These results indicated that the 4 synthetic peptides with ACE inhibitory activity were pH-stable, heat-stable, and resistant to proteinases in gastro-intestinal tracts. Therefore, those 4 peptides can be used as a source for functional food product with various applications.

Quality Evaluation of Low-fat Pork Loaf Containing Silkworm Powder and Vegetable Worm (Paecilomyces japonica) During Cold Storage

Aera Jang,Sang-Keun Jin,Cheorun Jo,Mooha Lee,Il-Suk Kim 한국식품과학회 2008 Food Science and Biotechnology Vol.17 No.4

This study was performed to determine physicochemical and sensory quality of low-fat pork loaf containing silkworm powder and vegetable worm (Paecilomyces japonica). Total 2% of fat replacer (soy protein isolate:maltodextrin:κ-carrageenan:water=1:0.5:0.5:10, w/v) was added. The loaf was separated into CTL (control), T1 (0.2% silkworm powder), T2 (0.2% vegetable worm), T3 (0.4% silkworm powder), T4 (0.4% vegetable worm), T5 (0.1% silkworm powder+0.1% vegetable worm), and T6 (0.2% silkworm powder+0.2% vegetable worm). Pork loaf of T3 showed the highest pH value and cooking loss of pork loaves containing silkworm and vegetable worm was higher than control at day 5. 2-Thiobarbituric acid reactive substances of T2 and T5 showed significantly lower values than control and those additives may reduce lipid oxidation of meat. Overall acceptability was not adversely influenced by silkworm powder and vegetable worm at day 0 and 5. These results indicated that those silkworm powder and vegetable worm could be utilized for pork product industry.