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과학적 근거 중심 평가를 도입한 마늘의 콜레스테롤 조절 기능성 평가
김인혜;조강진;김진영;황경아;황유진;채유진;엄애선 한양대학교 2010 韓國 生活 科學 硏究 Vol.30 No.1
Garlic (Allium sativum) is used a flavouring agent and a traditional medicine in human history. Numerous studies support the fact that garlic intake may have effect on cholesetrol-lowering. No claims currently have been made about garlic intake and cholesterol-lowering effect with respect to health claim in Korea. The aim of this study was to evaluate the scientific evidence on garlic intake and cholesterol-lowering effect by the scientific evidence-based rating system of Korean Food and Drug Administration for health claim. We carried out a comprehensive search of the Pubmed, EMBASE, Cochrane Library and manual searches of relevant journals for studies between 1955-2009. The search terms were garlic and Allium sativum in combination with cholesterol. Eighteen clinical stidies were included and reviewed to evaluate the strength of evidence that supports a relation between garlic intake and cholesterol-lowering effect. Our results suggests that level of scientific evidence on garlic intake and cholesterol-lowering effect is "other function (II) probable".
국내산 과일 및 채소 추출물을 이용한 체중 조절 관련 기능성 식품소재 탐색
김인혜;김종배;조강진;황유진;황경아;고정숙;엄애선 한양대학교 2011 韓國 生活 科學 硏究 Vol.31 No.1
Obesity can be defined as a disorder of lipid metabolism and the control of this process is regulated by enzymes involved in lipid metabolic pathway. Obesity is also characterized increases in the number and size of adipocyte differentiated from fibroblastic pre-adipocytes in adipose tissue. The aim of this study was to screen ethanol extracts of 21 fruits and vegetables cultivated in Republic of Korea for identifying natural sources of lipase inhibitor and to investigate the effects of several extracts resulting in suppression of triglyceride (TG) accumulation in 3T3-L1 cells. Among the tested extracts, Diospyros kaki L. showed the highest contents of total sugar and the content of total phenolic compounds was detected 17 species while there was not detected in Ixeris dentate root and Allium fistulosum C. etc. We were screened 26 extracts of fruits and vegetables for pancreatic lipase inhibitory activity compared with orlistat. The strongest lipase inhibitory activities were shown by extract of Allium sativum L. stem, Allium sativum L. bulb, Apteryx australis, Diospyros kaki L. Malus Pumila, Perilla frutescens and Prunus mume regardless of concentrations treated. Eight extracts including Brassica juncea var. integrilolia had no effect on lipase inhibition. During the differentiation of 3T3-L1 preadipocyte to mature adipocyte, the cells were treated with carrier or 7 extracts(1, 5 and 10 ㎍/ml), which was shown to highest lipase inhibitory effects. We were quantified lipid contents secreted mature adipocytes and stained with Oil Red O. All cells treated with the Apteryx australis and Prunus mume significantly decreased TG contents than the control. These findings suggested that the screened fruit and vegetable could be a promising source as the effective and safe lipase inhibitors for isolating bioactive ingredients and developing functional foods.
Hwang, Jee Youn,Kwon, Mun-Gyeong,Kim, Yu Jin,Jung, Sung-Hee,Park, Myoung-Ae,Son, Maeng-Hyun Elsevier 2017 Fish & shellfish immunology Vol.60 No.-
<P><B>Abstract</B></P> <P>Vaccination by immersion is suitable for mass vaccination of small size fish. However, no viral vaccine has been developed for immersion applications, because of low efficacy. In this study, we evaluated the efficacy and safety of immersion vaccine against viral hemorrhagic septicemia (VHS) containing Montanide IMS 1312 VG adjuvant in olive flounder (<I>Paralichthys olivaceus</I>). Healthy fish were vaccinated by an immersion method with a heat-inactivated FP-VHS2010-1 strain of VHS virus (VHSV) in combination with Montanide IMS 1312 VG for 5 min at 20 ± 2 °C. The control group was vaccinated with sterile PBS. No toxicity of immersion vaccine with Montanide IMS 1312 VG adjuvant was observed by hematological and histopathological analysis. Immersion vaccine with adjuvant enhanced gene expression of immune-associated genes, i.e., genes encoding interleukin (IL)-1β, IL-6, IL-8, and Toll-like receptor (TLR) 3. Relative percent survival (RPS) of fish was measured on weeks 4 and 8 post vaccination. In fish vaccinated with adjuvant, RPS was significantly higher than that of fish vaccinated without adjuvant. The results of the present study provide evidence that the VHSV immersion vaccine with Montanide IMS 1312 VG induces protective immunity in olive flounder against VHS.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Immersion vaccination of heat-inactivated VHSV with Montanide 1312 VG protected olive flounder from VHS. </LI> <LI> Immersion vaccination inceased the levels of several immune genes. </LI> <LI> Addition of Montanide 1312 VG further increased the levels of immune genes. </LI> <LI> Duration of protective effect of immersion vaccination was short. </LI> </UL> </P>
ELECTRICAL PROPERTIES OF Pb(Zr,Ti)O3 FERROELECTRIC CAPACITOR ON TiN/W PLUG STRUCTURE
Hwang, Sung-Yeon,Son, Young-Jin,Cho, Sung-Sil,Park, Sanghyun,Lee, Ae-Kyoung,Lee, Baek-Hee,Kim, Yoon-Jung,Park, Nam-Kyun,Park, Hae-Chan,Chang, HEON-YONG,Hong, Suk-Kyoung,Hong, Sung Joo,Choi, Ji-Hye,Kwe Taylor Francis 2007 Integrated ferroelectrics Vol.89 No.1
<P> We investigated the electrical properties of ferroelectric Pb(Zr,Ti)O3(PZT) capacitor on TiN/W storage node contact plug that is capacitor over bitline(COB) cell structure for a high density ferroelectric memory. In order to protecting TiN/W contact plug from oxidation failure during furnace annealing of PZT capacitor, the oxidation barrier of Ti1 - xAlxN (x∼ 0.25) film was interposed at between Pt/IrO2/Ir bottom electrode and TiN/W plug of the COB cell, resulting in the robust contact resistance property of 27 ohm per contact plug after furnace annealing performed at 600°C in O2 ambient for 90 min. The remanent polarization value and leakage current density of the fully integrated PZT capacitor with a top electrode area of 1.8 × 1.8 um2 on TiN/W contact plug showed 32 μ C/cm2 and 2.5 × 10- 6A/cm2, respectively.</P>
Hwang, Jee Youn,Kwon, Mun Gyeoung,Jung, Sung-Hee,Park, Myoung Ae,Kim, Dong-Wook,Cho, Wang Sik,Park, Jeong Woo,Son, Maeng-Hyun Elsevier 2017 FISH AND SHELLFISH IMMUNOLOGY Vol.62 No.-
<P><B>Abstract</B></P> <P>Viral hemorrhagic septicemia (VHS) is a highly contagious disease of cultured flounder caused by VHS virus (VHSV). To develop effective VHSV vaccines, it is essential to understand the molecular mechanisms underlying the host's protective response against VHSV. The purpose of this study is to clarify which genes are involved in the protective response of olive flounder after VHSV vaccination. We first injected olive flounder intraperitoneally with 10<SUP>7</SUP> TCID<SUB>50</SUB> heat-inactivated VHSV vaccine and evaluated the vaccine efficacy at 20 °C. Fish vaccinated with heat-inactivated VHSV were significantly protected compared to non-vaccinated fish, with a relative percentage survival of 83%. To analyze the vaccination-induced changes in the expression profiles of genes, kidneys were collected from control and vaccinated fish at days 1, 3, and 7 after vaccination and global gene expression profiling was carried out by RNA sequencing. The analysis revealed that 15,001 genes were differentially expressed by at least 2-fold between vaccinated fish and non-vaccinated controls. Of these, 58 genes clustered into the acute phase response, Toll-like receptor, interferon-inducible/regulatory proteins, and apoptosis pathways. These data provided insights into the molecular mechanisms underlying the protective immune response of olive flounder against heat-inactivated VHSV vaccine and might aid future studies to develop a highly immunogenic vaccine against VHSV in flounder.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Viral hemorrhagic septicemia virus (VHSV) is a major pathogen of cultured flounder. </LI> <LI> We analyzed the transcriptomic response to heat-inactivated VHSV in olive flounder. </LI> <LI> In total, 15,001 genes were differentially expressed after vaccination. </LI> <LI> Data analysis delineated 4 functional pathways responsive to VHSV vaccination. </LI> <LI> These findings can aid the future development of highly immunogenic VHSV vaccines. </LI> </UL> </P>
Induction of NQO1 and Neuroprotection by a Novel Compound KMS04014 in Parkinson's Disease Models.
Son, Hyo Jin,Choi, Ji Hyun,Lee, Ji Ae,Kim, Dong Jin,Shin, Kye Jung,Hwang, Onyou Birkhäuser Boston 2015 Journal of molecular neuroscience Vol.56 No.2
<P>Parkinson's disease (PD) is a progressive neurodegenerative disorder associated with a selective loss of the neurons containing dopamine (DA) in the substantia nigra pars compacta. Lines of evidence suggest that oxidative stress is a major factor contributing to the vulnerability of DA cells and that the enzyme NAD(P)H quinone oxidoreductase (NQO1) provides protection in these cells. In the present study, we report the synthesis of a novel compound KMS04014 and show that it induces NQO1 gene expression and protects DAergic neuronal cells in both cell culture and animal models of PD. In vitro, KMS04014 increased both mRNA and protein levels of NQO1 and induced nuclear translocation of Nrf2 in the DAergic neuronal cell line CATH.a. It also protected the cells against oxidative stress generated by tetrahydrobiopterin, 1-methyl-4-phenylpyridinium (MPP(+)), and H2O2. In vivo, KMS04014 attenuated the loss of tyrosine hydroxylase-immunopositive DAergic neurons in the substantia nigra and reduced degeneration of the nigral neurons and striatal fibers in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-treated mice, an animal model of PD. Taken together, KMS04014 may be utilized toward development of neuroprotective therapy for PD.</P>
Ji Ae Lee,권영원,Hye Ri Kim,Nari Shin,Hyo Jin Son,Chan Seong Cheong,Dong Jin Kim,Onyou Hwang 한국분자세포생물학회 2022 Molecules and cells Vol.45 No.3
The anti-oxidant enzyme heme oxygenase-1 (HO-1) is known to exert anti-inflammatory effects. From a library of pyrazolo[3,4-d]pyrimidines, we identified a novel compound KKC080096 that upregulated HO-1 at the mRNA and protein levels in microglial BV-2 cells. KKC080096 exhibited anti-inflammatory effects via suppressing nitric oxide, interleukin-1β (IL-1β), and iNOS production in lipopolysaccharide (LPS)-challenged cells. It inhibited the phosphorylation of IKK and MAP kinases (p38, JNK, ERK), which trigger inflammatory signaling, and whose activities are inhibited by HO-1. Further, KKC080096 upregulated anti-inflammatory marker (Arg1, YM1, CD206, IL-10, transforming growth factor-β [TGF-β]) expression. In 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-treated mice, KKC080096 lowered microglial activation, protected the nigral dopaminergic neurons, and nigral damage-associated motor deficits. Next, we elucidated the mechanisms by which KKC080096 upregulated HO-1. KKC080096 induced the phosphorylation of AMPK and its known upstream kinases LKB1 and CaMKKbeta, and pharmacological inhibition of AMPK activity reduced the effects of KKC080096 on HO-1 expression and LPS-induced NO generation, suggesting that KKC080096-induced HO-1 upregulation involves LKB1/AMPK and CaMKKbeta/AMPK pathway activation. Further, KKC080096 caused an increase in cellular Nrf2 level, bound to Keap1 (Nrf2 inhibitor protein) with high affinity, and blocked Keap1-Nrf2 interaction. This Nrf2 activation resulted in concurrent induction of HO-1 and other Nrf2-targeted antioxidant enzymes in BV-2 and in dopaminergic CATH.a cells. These results indicate that KKC080096 is a potential therapeutic for oxidative stress- and inflammation-related neurodegenerative disorders such as Parkinson’s disease.