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Bioremediation of Pb-Contaminated Soil Based on Microbially Induced Calcite Precipitation
( Achal Varenyam ),( Xiang Liang Pan ),( Dao Yong Zhang ),( Qing Long Fu ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.2
To remediate lead (Pb)-contaminated soils, it is proposed that microbially induced calcite precipitation (MICP) would provide the best alternative to other remediation technologies. In this study, Pb bioremediation in soils was investigated using the calcite-precipitating bacterium Kocuria flava. Results indicate that the Pb is primarily associated with the carbonate fraction in bioremediated soil samples. The bioavailability of Pb in contaminated soil was reduced so that the potential stress of Pb was alleviated. This research provides insight into the geochemistry occurring in the MICP-based Pb-remediated soils, which will help in remediation decisions.
KUKA youBot Integration with LabVIEW for Industrial Application
Achal Agarwal,Rajat Gupta,Vinayak Agarwal,Vijayant Saini 보안공학연구지원센터 2016 International Journal of Smart Home Vol.10 No.6
In this paper, we are going to explore how we have successfully implemented the interaction of KUKA youBot with LabVIEW using NI hardware C-RIO 9025, ether CAT cable and VGA cable. KUKA has recently launched a major research and development effort towards designing a mobile manipulation platform. We have been able to control the movement of all the five axis of youBot and gripper control. With the help of VGA cable we were able to establish connection between the youBot and PC to align youBot in its “open arm position”. Real time motion of youBot is achieved using C-RIO that is real-time embedded controller.
Characterization of Two Urease-Producing and Calcifying Bacillus spp. Isolated from Cement
( Achal Varenyam ),( Abhijit Mukherjee ),( M. Sudhakara Reddy ) 한국미생물 · 생명공학회 2010 Journal of microbiology and biotechnology Vol.20 No.11
Two bacterial strains designated as CT2 and CT5 were isolated from highly alkaline cement samples using the enrichment culture technique. On the basis of various physiological tests and 16S rRNA sequence analysis, the bacteria were identified as Bacillus species. The urease production was 575.87 U/ml and 670.71 U/ml for CT2 and CT5, respectively. Calcite constituted 27.6% and 31% of the total weight of sand samples plugged by CT2 and CT5, respectively. Scanning electron micrography analysis revealed the direct involvement of these isolates in calcite precipitation. This is the first report of the isolation and identification of Bacillus species from cement. Based on the ability of these bacteria to tolerate the extreme environment of cement, they have potential to be used in remediating the cracks and fissures in various building or concrete structures.
He, Jing,Zhang, Qiuzhuo,Achal, Varenyam The Korean Society for Microbiology and Biotechnol 2020 한국미생물·생명공학회지 Vol.48 No.2
The application of plant-growth-promoting rhizobacteria (PGPR) supports the growth of plants in contaminated soil while ureolytic bacteria can immobilise heavy metals by carbonate precipitation. Thus, dual treatment with such bacteria may be beneficial for plant growth and bioremediation in contaminated soil. This study aimed to determine whether the PGPR Pseudomonas fluorescens could work in synergy with ureolytic bacteria to assist with the remediation of cadmium (Cd)- and lead (Pb)-contaminated soils. Pot experiments were conducted to grow radish plants in Cd- and Pb-contaminated soils treated with PGPR P. fluorescens and the results were compared with dual inoculation of P. fluorescens combined with ureolytic Staphylococcus epidermidis HJ2. The removal rate of the metals from the soil was more than 83% for Cd and Pb by the combined treatment compared to 17% by PGPR alone. Further, the dual treatment reduced the metal accumulation in the roots by more than 80%. The translocation factors for Cd and Pb in plant tissues in both treatments remained the same, suggesting that PGPR combined with the carbonate precipitation process does not hamper the transfer of essential metal ions into plant tissues from the soil.
Experiences and Conceptualisation of Spinal Intramedullary Tuberculoma Management
Manish Jaiswal,Ashok Gandhi,Achal Sharma,Radhey Shyam Mittal 대한척추신경외과학회 2015 Neurospine Vol.12 No.1
Objective: Spinal intramedullary tuberculoma (SIMT) is rare, accounting for 2/100,000 cases of tuberculosis and only 0.2% of all cases of central nervous system(CNS) tuberculosis. We share our experiences of 11 cases of this entity for improving diagnosis and conceptualize the management of this rare disease. Methods: The clinical profile, radiological data and management of 11 cases of SIMT which were managed either conservatively or by surgical intervention during last 27 years (1987-2014) were analysed. Results: Male:female ratio was 1.75:1. Five cases had associated pulmonary Koch’s. Most common site was thoracic cord. Two cases had concurrent multiple intracranial tuberculoma. Most common presentation was paraparesis. X-ray myelography was performed in two patients in the initial period of study suggesting intramedullary pathology. In the subsequent nine cases who had magnetic resonance imaging (MRI), seven showed typical ‘‘target sign’’ and conglomerate ring lesion. Out of 8 surgically managed patients, 6 cases improved rapidly and in 2 patients gradual improvement was seen in follow-up. Most common indication of surgical excision was rapid neurological deterioration followed by diagnosis in doubt. Histopathology confirmed tuberculous etiology of the intramedullary lesion in all. Clinical and radiological improvement was seen in all 3 conservatively managed patients in follow-up. Conclusion: MRI findings of SIMT were specific and proven histologically correct. Surgical intervention may be indicated if there is no response to chemotherapy, the diagnosis is in doubt, or there is a rapid deterioration in neurological function because surgical outcome is good in these circumstances.
Cardiovagal Barorefex Sensitivity in Parkinson’s Disease and Multiple-System Atrophy
Sankanika Roy,Ashok Kumar Jaryal,Achal Kumar Srivastava,Kishore Kumar Deepak 대한신경과학회 2016 Journal of Clinical Neurology Vol.12 No.2
Background and Purpose Parkinson’s disease (PD) and multiple-system atrophy of the parkinsonian type (MSA-P) are progressive neurodegenerative disorders that in addition to dysfunction of the motor system also present with features of dysautonomia, frequently manifesting as orthostatic hypotension (OH). Te pathophysiology of OH has been proposed to difer between these two disorders. Tis study investigated the spontaneous and cardiovagal barorefex sensitivity (BRS) in Parkinson’s disease patients with orthostatic hypotension (PDOH ) and multiple system atrophy of Parkinsonian type with orthostatic hypotension in an attempt to diferentiate the two disorders. Methods Two methods were used for determining the BRS: a spontaneous method (spontaneous BRS) and the refexive barorefex gain (cardiovagal BRS) from phases II and IV of the Valsalva maneuver (VM) in PDOH and MSA-POH . Results The spontaneous BRS (5.04±0.66 ms/mm Hg vs. 4.78±0.64 ms/mm Hg, p=0.54) and the cardiovagal BRS from phase II of the VM (0.96±0.75 ms/mm Hg vs. 1.34±1.51 ms/mm Hg, p=0.76) did not difer between PDOH and MSA-POH , but the cardiovagal BRS from phase IV of the VM (0.03±0.07 ms/mm Hg vs. 2.86±2.39 ms/mm Hg, p=0.004) was signifcantly lower in PDOH . Conclusions Te cardiovagal BRS from phase IV of the VM has potential for diferentiating PDOH and MSA-POH , indicating a diference in the pathophysiological mechanisms underlying the autonomic dysfunction in the two disorders.
Jing He,Qiuzhuo Zhang,Varenyam Achal 한국미생물·생명공학회 2020 한국미생물·생명공학회지 Vol.48 No.2
The application of plant-growth-promoting rhizobacteria (PGPR) supports the growth of plants in contaminated soil while ureolytic bacteria can immobilise heavy metals by carbonate precipitation. Thus, dual treatment with such bacteria may be beneficial for plant growth and bioremediation in contaminated soil. This study aimed to determine whether the PGPR Pseudomonas fluorescens could work in synergy with ureolytic bacteria to assist with the remediation of cadmium (Cd)- and lead (Pb)-contaminated soils. Pot experiments were conducted to grow radish plants in Cd- and Pb-contaminated soils treated with PGPR P. fluorescens and the results were compared with dual inoculation of P. fluorescens combined with ureolytic Staphylococcus epidermidis HJ2. The removal rate of the metals from the soil was more than 83% for Cd and Pb by the combined treatment compared to 17% by PGPR alone. Further, the dual treatment reduced the metal accumulation in the roots by more than 80%. The translocation factors for Cd and Pb in plant tissues in both treatments remained the same, suggesting that PGPR combined with the carbonate precipitation process does not hamper the transfer of essential metal ions into plant tissues from the soil.