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Özdemir Erdi,Emet Abdülsamet,Hashemihesar Ramin,Yürüker Ali Celalettin Sinan,Kılıç Emine,Uçkan Çetinkaya Duygu,Turhan Egemen 한국조직공학과 재생의학회 2020 조직공학과 재생의학 Vol.17 No.6
Background: Articular cartilage repair has been a challenge in orthopedic practice due to the limited self-regenerative capability. Optimal treatment method for cartilage defects has not been defined. We investigated the effect of decellularized human placental (DHP) scaffold, mesenchymal stem cells (MSC) and platelet-rich plasma (PRP) on hyaline cartilage regeneration in a rat model. Methods: An osteochondral defect was created in trochlea region of the femur in all groups, bilaterally. No additional procedure was performed in control group (n = 14). Only the DHP scaffold was applied to the P group (n = 14). The DHP scaffold and 1 × 106 MSCs were applied to the PS group (n = 14). The DHP scaffold and PRP were applied to the PP group (n = 14). The DHP scaffold, 1 × 106 MSCs and PRP were applied to the PSP group (n = 14). Outcome measures at 12 weeks included Pineda histology score and qualitative histology. Results: The mean Pineda scores of P, PS, PP, and PSP groups were significantly better than the control group (p = 0.031, p = 0.002, p < 0.001, p < 0001, respectively). There was no statistically difference in mean Pineda scores of P, PS, PP, and PSP groups (p > 0.05). Conclusion: In conclusion, the DHP scaffold appears to be a promising scaffold on hyaline cartilage regeneration. The augmentation of DHP scaffold with MSCs and PRP combinations did not enhance its efficacy on articular cartilage regeneration.
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Potentiometric urea biosensor based on poly(glycidylmethacrylate)-grafted iron oxide nanoparticles
Emre Çevik,Mehmet S¸ enel,Abdülhadi Baykal 한국물리학회 2013 Current Applied Physics Vol.13 No.1
Urease enzyme was covalently attached on the poly(glycidylmethacrylate) (PGMA)-grafted iron oxide nanoparticles on Au electrode for the fabrication of urea biosensor. The telomere of poly(- glycidylmethacrylate) (PGMA) with a trimethoxysilyl terminal group was synthesized by telomerization of glycidylmethacrylate. Iron oxide nanoparticles were coated with telomere of poly(- glycidylmethacrylate) in order to obtain good enzyme immobilization platform. The telomere and nanoparticles were characterized by using Fourier transform infrared (FT-IR), X-ray diffraction (XRD), transmission electron microscopy (TEM) and thermal gravimetric analysis (TGA). The biosensor’s potentiometric response was measured as a function of urea concentration in phosphate buffer solution (10 mM, pH 7.5) and showed a linear range of 0.25e5.0 mM urea. The produced biosensor exhibited a good response time of w8 s and was stable for about two months. The basic features (optimum pH, optimum temperature, interference and storage stability) of the enzyme electrode were determined
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El-Rahman, Atef Abd El-Mohsen Abd,El-Shafei, Sally Mohamed Abd El-Aziz,Ivanova, Elena Vladimirovna,Fattakhova, Alfia Nurlimanovna,Pankova, Anna Victorovna,El-Shafei, Mohamed Abd El-Aziz,El-Morsi, El-M Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.17
Trichoderma spp. are known as a rich source of secondary metabolites with biological activity belonging to a variety of classes of chemical compounds. These fungi also are well known for their ability to produce a wide range of antibiotic substances and to parasitize other fungi. In search for new substances, which might act as anticancer agents, the overall objective of this study was to investigate the cytotoxic effects of Trichoderma harzianum and Trichoderma asperellum cultural filtrates against human cervical and breast cancer cell lines (HeLa and MCF-7 cells respectively). To achieve this objective, cells were exposed to 20, 40, 60, 80 and 100 mg/ml of both T. harzianum cultural filtrate (ThCF) and T. asperellum cultural filtrate (TaCF) for 24h, then the cell viability and the cytotoxic responses were assessed by using trypan blue and 3-(4,5-dimethylthiazol-2yl)-2,5-biphenyl tetrazolium bromide (MTT) assays. Morphological changes in cells were investigated by phase contrast inverted microscopy. The results showed that ThCF and TaCF significantly reduce the cell viability, have cytotoxic effects and alter the cellular morphology of HeLa and MCF-7 cells in a concentration dependent manner. A concentration of 80 and 100mg/ml of ThCF resulted in a sharp decline in the cell viability percent of HeLa and MCF-7 respectively (25.2%, 26.5%) which was recorded by trypan blue assay. The half-maximal inhibitory concentrations ($IC_{50}$) of ThCF and TaCF in HeLa and MCF-7 were recorded as 16.6, 12.0, 19.6 and 0.70mg/ml respectively by MTT assay. These results revealed that ThCF and TaCF have a substantial ability to reduce the viability and proliferation of human cervical and breast cancer cells.
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Abd El-Aty, Abd El-Aty M,Choi, Jeong-Heui,Park, Jong-Hyouk,Shim, Jae-Han P. Parey 2007 Berliner und Münchener tierärztliche Woc Vol.120 No.5
<P>The purpose of this study was to determine whether concurrent oral administration of activated charcoal has an affect on the depletion of the residual concentrations of enrofloxacin (ENRO) in chicken breast muscles. Sixty-four broiler chickens were divided into four groups (n = 16 per group), one given a daily oral dose of enrofloxacin with feed at a dose of 10 mg/kg for 5 consecutive days (control group) and the others given the same dose of enrofloxacin simultaneously with activated charcoal at a dose rate of 0.5, 1, and 2 % of daily feed for 5 days (treatment groups). At the end of treatment, 2 hens were sacrificed at each of the sampling time points (6,12, 18,48, 72,96,120 and 144 h after completion of dosing), breast muscles were collected and analyzed. Supercritical fluid extraction and high-performance liquid chromatography methods were used to determine the enrofloxacin residue levels in chicken breast muscles.The limit of quantification (LOQ) 16.5 microg/kg, was lower than the maximum residue levels (MRL) fixed by the Commission of the European Union. For all the time periods, charcoal treatment did not affect enrofloxacin tissue concentrations except at 12 and 48 h post treatment.To our knowledge, no studies on the depletion of enrofloxacin in the presence and absence of activated charcoal in chicken muscles have been performed. Although our current understanding is incomplete, multiple dose activated charcoals may play a role in the therapy of overdose.To prove this, further investigation is warranted.</P>
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Wafaa Abd El-Basit,Safaa Mohamed El-Ghanam,Ashraf Mosleh Abdel-Maksood,Sanaa Abd El-Tawab Kamh,Fouad Abd El-Moniem Saad Soliman 한국원자력학회 2016 Nuclear Engineering and Technology Vol.48 No.5
The present paper reports on a trial to shed further light on the characterization, applications, and operation of radar speed guns or Gunn diodes on different radiation environments of neutron or g fields. To this end, theoretical and experimental investigations of microwave oscillating system for outer-space applications were carried out. Radiation effects on the transient parameters and electrical properties of the proposed devices have been studied in detail with the application of computer programming. Also, the oscillation parameters, power characteristics, and bias current were plotted under the influence of different g and neutron irradiation levels. Finally, shelf or oven annealing processes were shown to be satisfactory techniques to recover the initial characteristics of the irradiated devices.
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Ahmed Abd El-badie Abd Allah K,Faten Abd El-Sattar Zahran El- 한국지능시스템학회 2020 INTERNATIONAL JOURNAL of FUZZY LOGIC and INTELLIGE Vol.20 No.4
Decision support systems improve medical diagnosis and minimize diagnostic errors. Existing diagnostic systems are often complex and exhibit limited performance on liver diseases, particularly the liver cancer. This paper presents a fuzzy decision support system for helping students diagnose some human liver diseases in educational medical institutions. The proposed system aims to improve real medical diagnosis processes. The approach has three basic steps: 1) symptoms-based diagnosis, 2) liver function-based diagnosis, and 3) image processing-based diagnosis. The proposed system employs two artificial intelligence techniques: fuzzy logic and image processing. The first is used for diagnosing liver diseases based on the liver function tests, while the second is used for diagnosing liver diseases such as the liver cancer, hepatitis, liver cirrhosis, liver fibrosis, and fatty liver. The proposed system combines two methods: the Mamdani inference and simulation method used in the MATLAB17 fuzzy logic toolbox, and the gray level co-occurrence matrix, for extracting the features of the second-order statistical texture of images acquired using computed tomography, magnetic resonance imaging, or ultrasound, for various liver diseases. Our results reveal a very good agreement between expert-made and system-made diagnoses, suggesting high accuracy.
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El-Mageed, Amal Abd El-Hafez Abd,Shawky Mohamed, Abd El-Aty,Elesawy, Basem Hasan Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.2
Background: Prognostication of breast cancer using clinico-pathologic variables, although useful, remains imperfect. Recent research has focused on finding new markers of prognosis using gene expression profiling. Panels of proteins assessed by immunohistochemistry might also be useful in this regard. This study focused on Bcl-2 protein expression in triple-negative (TNBC) and non- triple-negative breast cancer (non-TNBC) with correlation to clinico-pathologic variables. Materials and methods: We analyzed Bcl-2 expression in 77 women with primary breast carcinoma divided into two groups; triple-negative and non- triple-negative according to expression of estrogen (ER), progesterone (PR) and human epidermal growth factor receptors (Her2/neu). Bcl-2 expression was assessed in relation to age, histo-pathological subtype, grade, nodal status and tumor size. Results: Bcl-2 was expressed in 74% of triple-negative breast cancers and 70% of non- triple-negative cancers. In TNBC, expression was significantly correlated with invasive ductal subtype, while in non-TNBC it was significantly correlated with age and negative nodal status. In both groups higher Bcl-2 expression associated with favourable prognostic factors in breast cancer, but no significant statistical correlations were found. Conclusions: Frequency of Bcl-2 expression does not differ between TNBC and non-TNBC, but different prognostic factors correlate with Bcl-2 in the two cases.
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Measuring Performance in Egyptian Construction Firms Applying Quality Management Systems
Abd Elhamid, Manal S.,Ghareeb, Sahar.Sh. Korea Institute of Construction Engineering and Ma 2011 Journal of construction engineering and project ma Vol.1 No.2
Egyptian construction firms (ECF) started recently to adopt quality management system (QMS) as a way to develop and improve their performance as previous studies have shown. However, considering all the firm's aspects in a competitive way is a crucial process for the ECF's culture. This research is trying to indicate the role of the QMS implementation in measuring performance (MP) through developing a model for measuring performance at the organizational level, and to explore its impact on the organizations that adopt QMS. This model is based on specific elements and their related indicators which have been derived from national approaches and models of MP. This model determines the performance level (PL) of the organization that is measured by means of a point system. Weights of the elements in the point system considered both the elements' importance in the international models and its real practice in the ECFs. This model has been validated using another questionnaire and the majority of surveyed experts agreed that the proposed model can be applied effectively.
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Anaerobic codigestion of urban solid waste fresh leachate and domestic wastewaters
Abd-essamad Moujanni,Imane Qarraey,Aaziz Ouatmane 대한환경공학회 2019 Environmental Engineering Research Vol.24 No.1
The Biochemical Methane Potential (BMP) of fresh leachate and domestic wastewaters codigestion was determined by laboratory Bach Tests at 35ºC over a period of 90 d using a wide range of leachates volumetric ratios from 0% to 100%. To simulate wastewaters plant treatment step, all the ratios were first air stripped for 48 h before anaerobic incubation. The kinetic of biogas production was assessed using modified Gompertz model and exponential equation. The results obtained showed that cumulative biogas production was insignificant in the case of wastewaters monodigestion while the codigestion significantly improves the BMP. Air stripping pretreatment had positive effect on both ammonium concentration and volatiles fatty acids with reduction up to 75% and 42%, respectively. According to the Modified Gompertz model, the optimal anaerobic co-digestion conditions both in terms of maximal biogas potential, start-up period and maximum daily biogas production rate, could be achieved within large leachate volumetric ratios from 25% to 75% with a maximum BMP value of 438.42 mL/g volatile solid at 50% leachate ratio. The positive effect of codigestion was attributed to a dilution effect of chemical oxygen demand and volatile fatty acid concentrations to optimal range that was between 11.7 to 32.3 gO₂/L and 2.1 to 7.4 g/L, respectively. These results suggested that the treatment of fresh leachate by their dilution and co digestion at wastewaters treatment plants could be a promising alternative for both energetic and treatment purposes.
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