Gynecologic manifestation and identification of genetic causes of Klippel-Trenaunay syndrome

( A Mi Roh ),( Sa Ra Lee ),( Young Mee Lim ),( Kyung Ah Jeong ),( Hye Sung Moon ),( Hyewon Chung ) 대한산부인과학회 2018 대한산부인과학회 학술대회 Vol.104 No.-

Klippel-Trenaunay syndrome (KTS) is a congenital disorder characterized by malformations of the arteries, capillaries, veins and lymphatic system, surrounding soft tissue and bone hypertrophy. A. Timur et al. (2005) and others have reported that AGGF1 gene causes excessive angiogenesis in KTS. In addition, Fabio C. Vicentini (2006) and others introduced cases involving bladder and vulva. However, there has been no genetic study of KTS patients who have invaded the clitoris. In this study, whole genome sequencing of KTS patients and their families involving vulva were performed to identify KTS - related genes and to confirm genetic variants in KTS. A 26 year old woman visited OBGY clinic for protruding vaginal mass. On examination, the purplish protruding anterior vaginal wall was noted and a 1.5 x 1.5 cm sized clitoral mass was also noted. A sessile type elevated skin lesion causing itching and irritation symptom was noted on both buttock. She had suffered heavy menstrual bleeding and on pelvic MRI, a upward displacement of uterus was noted near umbilicus level. NSAIDs prescribed onthe first 3 days of menstruation decreased the menstrual blood loss upto 40percent. In this study, whole genome sequencing of a KTS patient involving vulva was performed to identify KTS - related genes and to confirm genetic variation. The patient's peripheral blood and a sessile mass around buttock was sampled and whole genome sequencing was performed. Most KTS has known to be a sporadic one, however some genetic variants has been reported.

Quality Characteristics of Jeju Beets Treated by Various Drying Conditions

A-Ra Ko,Hee-Jung Jin,Ji-Hyun Im,Jung-Hyun Nam,Ji-Yeon Chun 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

Beets (Beta vulgaris) contain a lot of betalains, a type of natural pigment, so they are known to be red and have strong antioxidant abilities. This study was conducted to compare the physicochemical properties of Jeju beets processed by hot air drying and combined drying. Beets were sliced to 3 mm and dried by hot air drying (13 hours at 50°C, 6 hours at 60°C, or 4.5 hours at 70°C) or combined drying (hot air and infrared radiation). Appearance, water activity, moisture content, pH, color, rehydration ratio (RR), total polyphenol content (TPC), FRAP value, DPPH radical scavenging ability, and ABTS radical scavenging ability were determined. In hot air drying, as the drying temperature increased, water activity, moisture content, and b* value were decreased while L*, a* value, RR, TPC, FRAP value, and ABTS radical scavenging ability were increased. The combined drying had lower water activity and pH value; moreover, it had a higher L*, a* value, RR, TPC, FRAP value, DPPH radical scavenging ability, and ABTS radical scavenging ability. In this study, it was found that the combined drying was more suitable than hot air drying to maintain the antioxidant ability of beet.

Inappropriate Gonadotropin Secretion in Polycystic Ovary Syndrome: The Relationship with Clinical, Hormonal and Metabolic Characteristics

( A Ra Shim ),( Yu Im Hwang ),( Kyung Jin Lim ),( Young Mi Choi ),( Young Eun Jeon ),( Seok Kyo Seo ),( Si Hyun Cho ),( Young Sik Choi ),( Byung Seok Lee ) 대한산부인과학회 2011 Obstetrics & Gynecology Science Vol.54 No.11

Objective To evaluate the clinical, hormonal and metabolic characteristics according to luteinizing hormone (LH)/follicle stimulating hormone (FSH) ratio in women with polycystic ovary syndrome (PCOS). Methods A total of 225 women with PCOS were included in this study. They were divided into two groups according to LH/FSH ratio; group A (LH/FSH < 2, n=160) and group B (LH/FSH > 2, n=65). We compared clinical, hormonal and metabolic characteristics including age at menarche, body mass index (BMI), blood pressures, ovarian volume, and serum androgen levels between the two groups. Serum glucose and insulin levels, fasting glucose/insulin ratio, homeostatic model assessment of insulin resistance (HOMA-IR), and lipid profi les were also compared between the two groups. Pearson correlation coeffi cients were used to evaluate correlation between the LH/FSH ratio and various parameters. Results Age of menarche in the group B was signifi cantly later than that in the group A. BMI, waist-hip ratio and blood pressures were signifi cantly lower in the group B compared to those of the group A. Ovarian volume and serum levels of LH, estradiol, total testosterone, sex-hormone binding globulin, 17-hydroxyprogesterone (OHP), and high density lipoprotein were signifi cantly higher in the group B. Postprandial 2 hours glucose, insulin and HOMA-IR were signifi cantly higher in the group A. After adjustment of BMI, the LH/FSH ratio was signifi cantly positively correlated with age at menarche, ovarian volume, total testosterone levels, and 17-OHP levels. Conclusion The inappropriate gonadotropin secretion may be negatively correlated with BMI, and positively with age of menarche and increased ovarian androgen production irrespective of BMI in women with PCOS. However, it may not be associated with metabolic characteristics.

Stability Analysis of a Family of Continuous State Feedback Synthesis: Theory and Experiments

Raúl Santiesteban-Cos,Araceli Gárate-García,Oscar Montaño-Godinez 제어·로봇·시스템학회 2017 International Journal of Control, Automation, and Vol.15 No.3

A family of continuous state feedback synthesis based on sliding mode control is analyzed using aLyapunov approach, such that the compensation of growing perturbations together with state variables is shown. Robustness properties of a family of controllers, varying from the well known twisting sliding mode control law tothe PD controller, are studied. A non-smooth Lyapunov function is proposed such that global finite-time stabilityof the origin is demonstrated and tuning rules for the control gains are obtained. Moreover, since the Lyapunovfunction is strict, an upper bound for the convergence time of the closed loop system can be estimated, in spite ofthe growing perturbations with respect to the state. To illustrate the performance and robustness properties of thefeedback synthesis, experimental results are presented, using a one-link pendulum as a test bed.

A plasma circulating miRNAs profile predicts type 2 diabetes mellitus and prediabetes: from the CORDIOPREV study

Rosa Jiménez-Lucena,Antonio Camargo,Juan Francisco Alcalá-Diaz,Cristina Romero-Baldonado,Raúl Miguel Luque,Ben van Ommen,Javier Delgado-Lista,Jose María Ordovás,Pablo Pérez-Martínez,Oriol Alberto Rang 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

We aimed to explore whether changes in circulating levels of miRNAs according to type 2 diabetes mellitus (T2DM) or prediabetes status could be used as biomarkers to evaluate the risk of developing the disease. The study included 462 patients without T2DM at baseline from the CORDIOPREV trial. After a median follow-up of 60 months, 107 of the subjects developed T2DM, 30 developed prediabetes, 223 maintained prediabetes and 78 remained disease-free. Plasma levels of four miRNAs related to insulin signaling and beta-cell function were measured by RT-PCR. We analyzed the relationship between miRNAs levels and insulin signaling and release indexes at baseline and after the follow-up period. The risk of developing disease based on tertiles (T1-T2-T3) of baseline miRNAs levels was evaluated by COX analysis. Thus, we observed higher miR-150 and miR-30a-5p and lower miR-15a and miR-375 baseline levels in subjects with T2DM than in disease-free subjects. Patients with high miR-150 and miR-30a-5p baseline levels had lower disposition index (p = 0.047 and p = 0.007, respectively). The higher risk of disease was associated with high levels (T3) of miR-150 and miR-30a-5p (HRT3-T1 = 4.218 and HRT3-T1 = 2.527, respectively) and low levels (T1) of miR-15a and miR- 375 (HRT1-T3 = 3.269 and HRT1-T3 = 1.604, respectively). In conclusion, our study showed that deregulated plasma levels of miR-150, miR-30a-5p, miR-15a, and miR-375 were observed years before the onset of T2DM and pre-DM and could be used to evaluate the risk of developing the disease, which may improve prediction and prevention among individuals at high risk for T2DM.

열풍 및 복합건조된 제주산 비트의 이화학적 특성과 항산화 활성

고아라(A-Ra Ko),남정현(Jung-Hyun Nam),진희정(Hee-Jung Jin),임지현(Ji-Hyun Im),김한상(Han-Sang Kim),천지연(Ji-Yeon Chun) 한국식품영양과학회 2022 한국식품영양과학회지 Vol.51 No.6

본 연구에서는 기존 비트의 한계점을 극복하고 고품질의 건조 비트를 제조하기 위한 기초 자료를 제시하고자 제주산 비트를 다양한 온도 조건에서 열풍 및 적외선 복합건조 하였고, 이에 따른 품질변화를 비교하여 건조 조건이 비트의 이화학적 특성과 항산화 활성에 미치는 영향에 대해서 조사하였다. 3 ㎜ 두께로 비트를 절단한 후 70℃에서 4.5시간, 60℃에서 6시간, 50℃에서 13시간 열풍건조 하거나 100℃에서 1시간 복합건조 하였다. 열풍건조한 비트는 건조 온도가 높아질수록 수분함량, 수분활성도, b<SUP>*</SUP>값(황색도), betanin 함량이 감소했고 건조수율과 pH에는 변화가 없었으며, L<SUP>*</SUP> 값(명도), a<SUP>*</SUP> 값(적색도), ΔE 값(건조 전 원물과의 색차), 재복원율, vulgaxanthin-Ⅰ 함량, 총 폴리페놀 함량, DPPH 라디칼 소거능, ABTS⁺ 라디칼 소거능, FRAP 활성은 증가하는 경향을 보였다. 그중에서 수분활성도, a<SUP>*</SUP>값, ΔE 값, FRAP 활성은 건조 온도가 높아짐에 따라 유의적으로 변화하였다. 복합건조한 비트는 열풍건조한 비트보다 L<SUP>*</SUP>값, a<SUP>*</SUP> 값, ΔE 값, 재복원율, 총 폴리페놀 함량, DPPH 라디칼 소거능, ABTS⁺ 라디칼 소거능, FRAP 활성이 유의적으로 높았고 pH는 유의적으로 낮았으며, 수분함량, 수분활성도, b<SUP>*</SUP>값, betanin 함량, vulgaxanthin-Ⅰ 함량에서는 열풍건조한 비트와 유의적인 차이를 나타내지 않았다. 이상의 결과에 따르면 복합건조한 비트가 다른 조건에서 건조를 진행한 비트에 비해 소요되는 시간이 매우 짧고 pH가 낮으며, 재복원율, 총 폴리페놀 함량, 항산화 활성이 높아 고품질의 건조 비트를 제조하기 위해서는 열풍뿐만이 아니라 적외선도 적절히 병용하는 것이 적합할 것으로 보인다. Beet (Beta vulgaris L.) is a healthy food that has recently attracted the consumer’s attention. However, beet has low storage stability due to the high moisture content. Therefore, a drying process is necessary to improve the beet quality. In this study, Jeju beet was dried by applying two processes: hot air drying (4.5 hours at 70℃, 6 hours at 60℃, 13 hours at 50℃) and combined drying (hot air and infrared radiation, 1 hour at 100℃). The effect of both processes was investigated on the physicochemical properties and antioxidant activity was investigated. The dried beets were compared for their appearance, drying yield, moisture content, water activity, pH value, color, rehydration ratio, betalain pigment content, total polyphenol content, DPPH radical scavenging activity, ABTS⁺ radical scavenging activity, and FRAP assay. The beet subjected to combined drying was determined to have high storage properties due to its low moisture content and pH value. In addition, the combined dried beet showed the highest rehydration ratio and antioxidant activity. Taken together, our results indicate that an appropriate combination of hot air and infrared radiation is suitable to produce high-quality dried beet.

Inhaled Loxapine as a Rapid Treatment for Agitation in Patients with Personality Disorder: A Prospective Study on the Effects of Time

Marc Ferrer,Óscar Soto-Angona,María Soler-Artigas,Pol Ibáñez,Christian Fadeuilhe,Raúl Felipe Palma-Álvarez,Raquefet Lidai,Sebastian Vargas-Cáceres,María Ángeles Torrecilla,Anna López,Josep Antoni Ramo 대한정신약물학회 2022 CLINICAL PSYCHOPHARMACOLOGY AND NEUROSCIENCE Vol.20 No.3

Objective: Agitation in patients diagnosed with personality disorders (PD) is one of the most frequent crises in emergency departments (ED). Although many medications have been tested, their effectiveness has been small or non-significant, and no specific drugs are supported by the available evidence. This study aimed to evaluate the efficacy of Inhaled loxapine (IL) as a therapeutic option for agitated patients with PD. Methods: A naturalistic, unicentric, prospective study was carried out. Thirty subjects diagnosed with PD and attending the ED with episodes of agitation were recruited most of whom were women diagnosed with Borderline Personality Disorder. Subjects were treated with a single dose of IL (9.1 mg). Efficacy was assessed with the Clinical Global Impression scale, the Excited Component of the Positive and Negative Syndrome Scale (PANSS-EC) and the Agitation-Calmness Evaluation Scale (ACES). Patients were followed 60 minutes after administration to measure IL effect and its duration. Results: IL exhibited an overall efficacy in managing mild to severe agitation, with a quick onset of effect and persistence. ‘Effect of time’, where IL efficacy is maintained over time, is more marked in higher-severity agitation. No additional treatments were needed to improve agitation during the follow-up time. Conclusion: Results suggest that IL could be a safe and effective option to manage agitation in PD.

Low-Scale Expression and Purification of an Active Putative Iduronate 2-Sulfate Sulfatase-Like Enzyme from Escherichia coli K12

Edwin David Morales-Álvarez,Claudia Marcela Rivera-Hoyos,Angélica María Baena-Moncada,Patricia Landázuri,Raúl A. Poutou-Piñales,Homero Sáenz-Suárez,Luis A. Barrera,Olga Y. Echeverri-Peña 한국미생물학회 2013 The journal of microbiology Vol.51 No.2

The sulfatase family involves a group of enzymes with a large degree of similarity. Until now, sixteen human sulfatases have been identified, most of them found in lysosomes. Human deficiency of sulfatases generates various genetic disorders characterized by abnormal accumulation of sulfated intermediate compounds. Mucopolysaccharidosis type II is characterized by the deficiency of iduronate 2-sulfate sulfatase (IDS), causing the lysosomal accumulation of heparan and dermatan sulfates. Currently, there are several cases of genetic diseases treated with enzyme replacement therapy, which have generated a great interest in the development of systems for recombinant protein expression. In this work we expressed the human recombinant IDS-Like enzyme (hrIDS-Like) in Escherichia coli DH5α. The enzyme concentration revealed by ELISA varied from 78.13 to 94.35 ng/ml and the specific activity varied from 34.20 to 25.97 nmol/h/mg. Western blotting done after affinity chromatography purification showed a single band of approximately 40 kDa, which was recognized by an IgY polyclonal antibody that was developed against the specific peptide of the native protein. Our 100 ml-shake-flask assays allowed us to improve the enzyme activity seven fold,compared to the E. coli JM109/pUC13-hrIDS-Like system. Additionally, the results obtained in the present study were equal to those obtained with the Pichia pastoris GS1115/pPIC-9-hrIDS-Like system (3 L bioreactor scale). The system used in this work (E. coli DH5α/pGEX-3X-hrIDS-Like)emerges as a strategy for improving protein expression and purification, aimed at recombinant protein chemical characterization,future laboratory assays for enzyme replacement therapy, and as new evidence of active putative sulfatase production in E. coli.

Changes in plasma lipoxin A4, resolvins and CD59 levels after ischemic and traumatic brain injuries in rats

Jun-Sub Jung,A Ra Kho,Song Hee Lee,Bo Young Choi,Shin-Hae Kang,Jae-Young Koh,Sang Won Suh,Dong-Keun Song 대한생리학회-대한약리학회 2020 The Korean Journal of Physiology & Pharmacology Vol.24 No.2

Ischemic and traumatic brain injuries are the major acute central nervous system disorders that need to be adequately diagnosed and treated. To find biomarkers for these acute brain injuries, plasma levels of some specialized pro-resolving mediators (SPMs, i.e., lipoxin A4 [LXA4], resolvin [Rv] E1, RvE2, RvD1 and RvD2), CD59 and interleukin (IL)-6 were measured at 0, 6, 24, 72, and 168 h after global cerebral ischemic (GCI) and traumatic brain injuries (TBI) in rats. Plasma LXA4 levels tended to increase at 24 and 72 h after GCI. Plasma RvE1, RvE2, RvD1, and RvD2 levels showed a biphasic response to GCI; a significant decrease at 6 h with a return to the levels of the sham group at 24 h, and again a decrease at 72 h. Plasma CD59 levels increased at 6 and 24 h post-GCI, and returned to basal levels at 72 h post-GCI. For TBI, plasma LXA4 levels tended to decrease, while RvE1, RvE2, RvD1, and RvD2 showed barely significant changes. Plasma IL-6 levels were significantly increased after GCI and TBI, but with different time courses. These results show that plasma LXA4, RvE1, RvE2, RvD1, RvD2, and CD59 levels display differential responses to GCI and TBI, and need to be evaluated for their usefulness as biomarkers.

Determination of volatile organic compounds generated from fresh, white and red Panax ginseng (C. A. Meyer) using a direct sample injection technique

Abd El-Aty, A. M.,Kim, In-Kyung,Kim, Mi-Ra,Lee, ChiHo,Shim, Jae-Han John Wiley Sons, Ltd. 2008 Biomedical chromatography Vol.22 No.5

<P>Ginsenosides are regarded as the main active, non-volatile components of Panax ginseng (C. A. Meyer). However, throughout the long history of ginseng research, there has been virtually no report describing its volatile flavor compounds. A solvent-free procedure for the determination of volatile flavor compounds generated from fresh, white and red Panax ginseng (C. A. Meyer) using solvent-free solid injection (SFSI) coupled with gas chromatography-mass spectrometry (GC-MS) detection is described here. At no point in the SFSI technique were the extraction conditions optimized. Rather, the experimental variables including various sample preparations (fresh, oven-dried and freeze-dried), injector temperatures (100, 150, 200, 250 and 300°C), and preheating times (3, 5, 7, 10 and 15 min), were predicated on the experience of the authors. A total of 47 compounds were identified in various forms of ginseng. Among the compounds identified in the sample, fresh ginseng was characterized by a high proportion of 3-acetyl-1-(3,4-dimethoxyphenyl)-5-ethyl-4,5-dihydro-7,8-dimethoxy-4-methylene-3H-2,3-benzodiazepine (64.24%) and 23,24-dinor-3-oxolean-4,12-dien-28-oic acid (21.42%); 2-furanmethanol (20.26%) and 3-hydroxy-2-methyl-4H-pyran-4-one (17.95%) were detected as the major components in white ginseng while the main components of the red ginseng were found to be 1,2-benzenedicarboxylic acid dibutyl ester (16.27%) and 2-furanmethanol (13.82%). SFSI is a solvent-free, rapid and simple sample preparation technique based on direct vaporization. There is no dilution or contamination with solvent or its impurities and no loss of quickly eluted components was observed in the solvent peak. Copyright © 2008 John Wiley & Sons, Ltd.</P>