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Carolina A. Lima,José L. Lima Filho,Benício B. Neto,Attilio Converti,Maria G. Carneiro da Cunha,Ana L. F. Porto 한국생물공학회 2011 Biotechnology and Bioprocess Engineering Vol.16 No.3
A 2^4 full factorial design was used to identify the main effects and interactions of the initial medium pH, soybean flour concentration, temperature and orbital agitation speed on extracellular collagenase production by Penicillium aurantiogriseum URM4622. The most significant variables for collagenase production were soybean flour concentration and initial medium pH that had positive main effects, and temperature that had a negative one. Protein concentration in soybean flour revealed to be a significant factor for the production of a collagenase serine proteinase. The most favorable production conditions were found to be 0.75% soybean flour, pH 8.0, 200 rpm, and 28ºC, which led to a collagenase activity of 164 U. The enzyme showed an optimum activity at 37℃ and pH 9.0,was stable over wide ranges of pH and temperature (6.0 ~10.0 and 25 ~ 45℃, respectively) and was strongly inhibited by 10 mM phenylmethylsulphonylfluoride. The firstorder rate constants for collagenase inactivation in the crude extract, calculated from semi-log plots of the residual activity versus time, were used in Arrhenius and Eyring plots to estimate the main thermodynamic parameters of thermoinactivation (E^*_d = 107.4 kJ/mol and ΔH^*_d = 104.7kJ/mol). The enzyme is probably an extracellular neutral serine collagenase effective on azocoll, gelatin and collagen decomposition.
Balhaddad Abdulrahman A.,Garcia Isadora,Collares Fabrício,Felix Cristopher M.,Ganesh Nisha,Alkabashi Qoot,Massei Ward,Strassler Howard,Melo Mary Anne 대한치과보존학회 2020 Restorative Dentistry & Endodontics Vol.45 No.4
Objectives: This study investigated the effects of physically damaged and resin-contaminated tips on radiant emittance, comparing them with new undamaged, non-contaminated tips using 3 pieces of spectrophotometric laboratory equipment. Materials and Methods: Nine tips with damage and/or resin contaminants from actual clinical situations were compared with a new tip without damage or contamination (control group). The radiant emittance was recorded using 3 spectrophotometric methods: a laboratory-grade thermopile, a laboratory-grade integrating sphere, and a portable light collector (checkMARC). Results: A significant difference between the laboratory-grade thermopile and the laboratory-grade integrating sphere was found when the radiant emittance values of the control or damaged/contaminated tips were investigated (p < 0.05), but both methods were comparable to checkMARC (p > 0.05). Regardless of the method used to quantify the light output, the mean radiant emittance values of the damaged/contaminated tips were significantly lower than those of the control (p < 0.05). The beam profile of the damaged/contaminated tips was less homogeneous than that of the control. Conclusions: Damaged/contaminated tips can reduce the radiant emittance output and the homogeneity of the beam, which may affect the energy delivered to composite restorations. The checkMARC spectrophotometer device can be used in dental offices, as it provided values close to those produced by a laboratory-grade integrated sphere spectrophotometer. Dentists should assess the radiant emittance of their light-curing units to ensure optimal curing in photoactivated, resin-based materials.
Wagner L. Gonçalves,Guilherme F. Gomes,Yohan D. Mendéz,Fabrício A. Almeida,Valquíria C. Santos,Sebastião S. Cunha Jr. 사단법인 한국계산역학회 2018 Computers and Concrete, An International Journal Vol.22 No.1
Reinforced concrete structures are widely used in civil engineering projects around the world in different designs. Due to the great evolution in computational equipment and numerical methods, structural analysis has become more and more reliable, and in turn more closely approximates reality. Thus among the many numerical methods used to carry out these types of analyses, the finite element method has been highlighted as an optimized tool option, combined with the non-linear and linear analysis techniques of structures. In this paper, the behavior of reinforced concrete beams was analyzed in two different configurations: i) with welding and ii) conventionally lashed stirrups using annealed wire. The structures were subjected to normal and tangential forces up to the limit of their bending resistance capacities to observe the cracking process and growth of the concrete structure. This study was undertaken to evaluate the effectiveness of welded wire fabric as shear reinforcement in concrete prismatic beams under static loading conditions. Experimental analysis was carried out in order compare the maximum load of both configurations, the experimental load-time profile applied in the first configuration was used to reproduce the same loading conditions in the numerical simulations. Thus, comparisons between the numerical and experimental results of the welded frame beam show that the proposed model can estimate the concrete strength and failure behavior accurately.
Fabrício Eduardo Rossi,Ana Claudia S. Fortaleza,Lucas M. Neves,Tiego A. Diniz,Marcela R. de Castro,Camila Buonani,Jorge Mota,Ismael F. Freitas Junior 한국운동재활학회 2017 JER Vol.13 No.3
The aim of this study was to compare the effects of combined (CT; strength plus aerobic) and functional training (FT) on the body composition and metabolic profile with a similar training load in postmenopausal women. The participants were divided into three groups: CT (n= 20), FT (n= 17), and control group (CG, n= 15). The trunk FM, fat mass (FM), percentage of FM (FM%), and fat-free mass were estimated by dual-energy X-ray absorptiometry. The metabolic profile, glucose, triacylglycerol, total cholesterol, high-density lipoprotein cholesterol and low-density lipoprotein cholesterol (LDL-c) were assessed. There were main effects of time in trunk fat, FM, and FM% (P< 0.05). There were statistically significant interaction for FM (P= 0.015), FM% (P= 0.017) with lower values for CT group. For LDL-c, there was significant interaction (P= 0.002) with greater values for FT group in relation to CG and CT. Furthermore, when performed the post hoc test on the “mean absolute differences” (Δ), it can observed statistically significant difference between FT, CT, and CG (-13.0± 16.5 mg/dL vs. 4.8± 18.4 mg/dL vs. 9.2± 18.8 mg/dL, P< 0.05). In conclusion, when training loads are equivalent CT potentiated a reduction in FM and FM%, however, only FT reduced LDL-c in postmenopausal women.
Statistical bias indicators for the long-term displacement of steel-concrete composite beams
Julian A. Moreno,Jorge L.P. Tamayo,Inácio B. Morsch,Marcela P. Miranda,Lucas H. Reginato 사단법인 한국계산역학회 2019 Computers and Concrete, An International Journal Vol.24 No.4
Steel-concrete composite beams are widely employed in constructions and their performance at the serviceability stage is of concern among practitioners and design regulations. In this context, an accurate evaluation of long-term deflections via various rheological concrete models is needed. In this work, the performance and predict capability of some concrete creep and shrinkage models ACI, CEB, B3, FIB and GL2000 are ascertained, and compared by using statistical bias indicators. Ten steel-concrete composite beams with existing experimental and numerical results are then modeled for this purpose. The proposed modeling technique uses the finite element method, where the concrete slab and steel beam are modeled with shell finite elements. Concrete is considered as an aging viscoelastic material and cracking is treated with the common smeared approach. The results show that when the experimental ultimate shrinkage strain is used for calibration, all studied rheological models predict nearly similar deflections, which agree with the experimental data. In contrast, significance differences are encountered for some models, when none calibration is made prior to. A value between twenty and thirty times the cracking strain is recommended for the ultimate tensile strain in the tension stiffening model. Also, increasing the relative humidity and decreasing the ambient temperature can lead to a substantial reduction of slab cracking for beams under negative flexure. Finally, there is not a unique rheological model that clearly excels in all scenarios.
The uniaxial strain test - a simple method for the characterization of porous materials
J. Grácio,T. Fiedler,A. Öchsner 국제구조공학회 2006 Structural Engineering and Mechanics, An Int'l Jou Vol.22 No.1
The application of cellular materials in load-carrying and security-relevant structures requires the exact prediction of their mechanical behavior, which necessitates the development of robust simulation models and techniques based on appropriate experimental procedures. The determination of the yield surface requires experiments under multi-axial stress states because the yield behavior is sensitive to the hydrostatic stress and simple uniaxial tests aim only to determine one single point of the yield surface. Therefore, an experimental technique based on a uniaxial strain test for the description of the influence of the hydrostatic stress on the yield condition in the elastic-plastic transition zone at small strains is proposed and numerically investigated. Furthermore, this experimental technique enables the determination of a second elastic constant, e.g., Poisson’s ratio.
Balhaddad Abdulrahman A.,Garcia Isadora M.,Maktabi Haifa,Ibrahim Maria Salem,Alkhubaizi Qoot,Strassler Howard,Collares Fabrício M.,Melo Mary Anne S. 대한치과보존학회 2021 Restorative Dentistry & Endodontics Vol.46 No.4
Objectives This study aimed to evaluate the effect of improper positioning single-peak and multi-peak lights on color change, microhardness of bottom and top, and surface topography of bulk fill and incremental composites after artificial aging for 1 year. Materials and Methods Bulk fill and incremental composites were cured using multi-peak and single-peak light-emitting diode (LED) following 4 clinical conditions: (1) optimal condition (no angulation or tip displacement), (2) tip-displacement (2 mm), (3) slight tip angulation (α = 20°) and (4) moderate tip angulation (α = 35°). After 1-year of water aging, the specimens were analyzed for color changes (ΔE), Vickers hardness, surface topography (Ra, Rt, and Rv), and scanning electron microscopy. Results For samples cured by single-peak LED, the improper positioning significantly increases the color change compared to the optimal position regardless of the type of composite (p < 0.001). For multi-peak LED, the type of resin composite and the curing condition displayed a significant effect on ΔE (p < 0.001). For both LEDs, the Vickers hardness and bottom/top ratio of Vickers hardness were affected by the type of composite and the curing condition (p < 0.01). Conclusions The bulk fill composite presented greater resistance to wear, higher color stability, and better microhardness than the incremental composite when subjected to improper curing. The multi-peak LED improves curing under improper conditions compared to single-peak LED. Prevention of errors when curing composites requires the attention of all personnel involved in the patient's care once the clinical relevance of the appropriate polymerization reflects on reliable long-term outcomes.
Lentinula edodes (Shiitake) Modulates Chemically Induced Mutagenesis by Enhancing Pitting
Patrı´cia L. Alves de Lima,Marina M. Sugui,Angela I.M. Petrı´cio,Lı´zia C. Vilela,Andre´a V.F. Pinto,Priscila R. Martins,Ramon Kaneno,Daniel A. Ribeiro,Daisy M.F. Salvadori,Lu´cia R. Ribeiro 한국식품영양과학회 2013 Journal of medicinal food Vol.16 No.8
This study was undertaken to understand how Lentinula edodes modulates in vivo mutagenesis induced by alkylating agents in bone marrow and peripheral blood as described in our previous article. Male Swiss mice were pretreated for 15 consecutive days with aqueous extracts prepared from L. edodes, after which, the number of circulating blood cells, normal erythroid bone marrow cell cycling, and phagocytosis of micronucleated reticulocyte (MNRET) and activation of spleen macrophages were assessed. The results indicate that the antimutagenicity seen in bone marrow and peripheral blood is exerted by distinct compounds with different actions. The antimutagenic effect in bone marrow is exerted by compounds subject to degradation at deep-freeze storage temperature of - 20 C. On the other hand, compounds responsible for antimutagenicity in peripheral blood are not subject to degradation at - 20 C. The results also indicate that the antimutagenic action in peripheral blood leading to the reduction of circulating MNRET occurs in the spleen primarily through a phagocytic activity due to higher macrophage numbers and probably not due to the enhanced activation state of individual cells.