D-Amino Acid Aminotransferase 활성보유 고온성미생물의 탐색 및 분류학적 특성 연구

곽미선,이승구,정상철,서승현,이재흥,전영중,김영호,성문희 충남대학교 약학대학 의약품개발연구소 1999 藥學論文集 Vol.15 No.-

To acquire an industrially useful biocatalyst for the enzymatic synthesis and production of various D-amino acids, 1,300 thermophiles isolated from the soil of Korea were analyzed for D-amino acid aminotransferase (D-AAT) activity. The enzyme activity was found from 110 strains of isolated thermophiles revealing its wide occurrence in thermophiles. Enzyme activity and thermal stability of the D-AAT producers were compared. Finally we have selected four thermophiles as producers of potent biocatalysts for the D-amino acid production; two therrnophiles, Bacillus sp. LK-I and LK-2, having higher specific activity and two thermophiles, B. stearothermophilus KL-01 and Bacillus sp. KLS-0l, having higher thermal stability than the D-AAT producers. Taxonomic and physiological characteristics of the four isolated thermophiles were described herein.

자폐장애 환자에서 FMR-1 유전 삼염기 반복의 분자생물학적 분석

곽호순,정철호,전효진,장은진,김희철,김정범,박영남 대한소아청소년정신의학회 2000 소아청소년정신의학 Vol.11 No.1

연구목적 : 자폐장애의 원인을 유전학적으로 규명하려는 연구가 시도되고 있으며, 그 중 fragile-X 증후군과의 연관성에 대한 연구가 활발히 진행되고 있다. fragile-X 염색체(Xq27.3)는 세포유전학적 방법으로 증명할 수 있으나 검사에 많은 제약과 단점이 있으므로, 본 연구에서는 보다 신뢰성이 높은 분자 생물학적 방법으로 FMR-1 유전자내 CGG 삼염기 반복부위를 분석하여 자폐장애와 fragile-X 증후군의 연관성을 규명하고자 하였다. 방법 : 자폐장애 환아(99명)와 정상대조군(8명)을 대상으로 FMR-1 유전자의 CGG 반복배열 부위를 sense와 antisense primer를 이용하여 PCR법으로 분석하엿으며, 동시에 세포유전학적 검사도 시행하였다. PCR 분석에서 CGG 반복수가 50 이상인 경우에 대해서는 StB12.3 혹은 Pfxa3 probe를 이용한 Sourthern blot hybridization으로 확인하였다. 결과 : FMR-1 유전자의 CGG 반복배열에 대한 PCR 분석 결과 CGG 삼염기의 반복배열의 수는 자폐장애 환자군과 정상대조군 사이에 통계적으로 유의한 차이가 없었다(p=0.207). 자폐장애 환자에서 CGG 반복수가 50회 이상인 조기변이(premutation) 환자가 2명 있었으나 Sourthern blot hybridization 결과 완전변이(full mutation)로 판정할 수 있는 경우는 없었다. 세포유전학적 검사에서 환자군 모두에서 정상 핵형을 나타내었으며 fragile-X 염색체는 확인되지 않았다. 결론 : 이상의 결과에서 자페장애 환자가 FMR-1 유전자의 CGG 삼염기 반복부위 이상, 즉 fragile-X 염색체 이상을 동반하지 않았음을 증명할 수 있었다. 이는 fragile-X 증후군을 자�장애의 직접적인 원인이라고 보기에는 어려움이 있음을 시사한다. Objectives : There has been a rapid expansion of studies aimed at elucidating the genetic basis of autistic disorder, especially it's relationship to fragile-X syndrome. The detection of fragile X chromosome(Xq27.3) by cytogenetic analysis has revealed many difficulties in testing. Therefore, to explore the relationship between autistic disorder and fragile X syndrome, this study administered molecular biologic methods which examined an unstable CGG repeat within the fragile X mental retardation-1(FMR-1) gene. Methods : Ninety nine autistic children and eight normal control children were tested. The number of CGG repeats within FMR-1 gene was measured after amplification by PCR, and cytogenetic analysis was also carried out to detect fragile site Xq27.3. Sourthern blot hybridization, using StB12.3 and/or Pfxa3 probe, was done for the patients showing expansion of more than 50 CGG repeats(premutation). Results : All but two autistic patients had no expansion in CGG repeats by PCR and there was no significant statistical difference in number of CGG repeat in comparison with normal control. Two autistic patients, considered as premutation by PCR analysis, had no full mutation or premutation by Southern blot hybridization. All autistic children tested did not have any abnormal karyotype or fragile site Xq27.3. Conclusions : These results suggest that autistic patients may not have abnormality in FMR-1 gene or abnormal expansion in CGG repeat. In conclusion, fragile X syndrome may not be antecedent of autistic disorder. KEY WORDS : Autistic disorder·FMR-1 gene trinucleotide repeats·Molecular biologic analysis.

주정 중독 흰쥐에서 총담관 결찰이 간 및 혈청의 Glyoxalase I 활성에 미치는 영향

곽춘식,김여희,변용준 啓明大學校 醫科大學 1995 계명의대학술지 Vol.14 No.4

The activities of the rat liver and serum glyoxalase-I(GLO-I) were studied in cholestasis induced by common bile duct(CBD) ligation after chronic ethanol intoxication, and in cholestasis before acute ethanol intoxication to establish the biochemical background of alcohol hazards in hepatobiliary disease. GLO-I activities in the rat's liver and serum showed less increases when CBD was ligated after chronic ethanol intoxication than that in the CBD ligation alone. GLO-I activities in the rat's liver and serum showed no more increases when acute ethanol intoxication was induced after CBD ligation than that in the CBD ligation alone. On the other hand, when CBD was ligated after chronic ethanol intoxication, the value of Vmax of the liver GLO-I decreased significantly than that in the CBD was ligated alone. However, the values of Km of the liver GLO-I did not changes in the all experimental groups. Viewed from above results, when chronic ethanol intoxication is combined with cholestasis, GLO-I in the liver seems to be decrease its activity than that in cholestasis, and the cause of the decrease is thought to be decreased biosynthesis.

닭과 대구를 첨가한 족편의 관능적특성

곽은정,안효진,이경희,이영순 한국조리과학회 2002 한국식품조리과학회지 Vol.18 No.6

We examined the quality characteristics and texture of Jokpyuns with chicken and codfish. In proportion to the increase of water addition, the color became lighter and the texture characteristics decreased in So-Jokpyuns, which were prepared with cow feet. The texture and taste had an influence on overall preference of So-Jokpyun. Yongbon-Jokpyun which was prepared with cow feet and chicken was much preferred to So-Jokpyun in the appearance and texture, and showed a similar preference with So-Jokpyun. However, Sodaegu-Jokpyun which was prepared with cow feet and codfish was not preferred to So-Jokpyun in all items including appearance and texture, and the overall preference was remarkably low. Overall preference of Yongbong-Jokpyun and Sodaegu-Jokpyun was greatly related with the preference of taste and flavor. The lightness and the degree of yellow color of Yongbong-Jokpyun were higher and the degree of red color was lower than So-Jokpyun. On the other hand, the results of lightness, degree of red and yellow color in Sodaegu-Jokpyun were opposite to those of Yongbong-Jokpyun. In case of the hardness, Yongbong-Jokpyun was the lowest and Sodaegu-Jokpyun was similar with So-Jokpyun. Adhesiveness of Yongbong-Jokpyun and Sodaegu-Jokpyun was lower than that of So-Jokpyun. There was no difference in the springiness and cohesiveness among 3 kinds of Jokpyun.

HG-AAS에 의한 음용수중의 세레늄분석에 관한 연구

곽영규,김철,천미희 동의공업대학 2000 論文集 Vol.26 No.1

This paper describes a adequate pretreatment method for determining selenium by atomic absorption spectrometry with hydride generation (HG-AAS). The concentration of selenium was determined by HG-AAS, in which selenium was not detected, but selenite was measured. For a total selenium determination, selenate should be reduced to selenite by boiling a few minutes to sample solutions with reducing reagent. After a variable study, its condition was determined which 30m1 sample solution is boiling for 30min with hydrochloric acid (FW: 36.46, 35%) in lOO℃ water bath. As a recovery result in this method to deionized water and drinking water, almost 100% of selenate and selenite were recovered from deionized water, above 97% from drinking water.

고전압 급준펄스의 오버슈트 개선에 관한 연구

곽희로,권동진,김영찬 崇實大學校 生産技術硏究所 1994 論文集 Vol.24 No.-

This paper describes very fast rising high voltage pules generation using the traveling wave theory of the transmission line. This very fast rising high voltage pulse generator consists of a charging coaxial cable, a discharging switch, and a terminating resistance. The results of the study show that the rising time of pulses is very fast about 30 [nsec]. The length of the coaxial cable and the charging voltage can regulate the duration and the amplitude or the polarity of the pulse. In addition, rod to plane electrodes are suitable as a short switch and the overshoot of pulse was improved with the larger radius of the rod electrode.

관상 동맥 질환에서 아포 E 지단백 유전자 다형성과 혈청 지질치와의 관계

곽선영,김성구,정호석,이유경,이광희,김철현,최태명,현민수,권영주 순천향의학연구소;Soonchunhyang Medical Research Institute 2000 Journal of Soonchunhyang Medical Science Vol.6 No.1

Background and aims: The Apolipoprotein E is a ligand of both the protein component LDL receptor as well as the apo E LDL-Receptor related protein (LRP). It modulates the receptor binding of lipoproteins, with the apolipoprotein E found on cell surfaces as its component, thus serving an important role in the lipid metabolism by carrying out the intracellular transport of cholesterol in lipoproteins. The gene for apolipoprotein E is the product of three common genotypes as well as many more rare alleles. The common genotypes are ε2, ε3, and ε4, and are expressed in the three phenotype isoforms of E2, E3, and E4. In the event that E4 is the main component, a rise in the cholesterol level, as the result of down-regulation of the LDL receptor, is observed. Therefore, those samples with E4 genotypes are known to be in much higher risk of coronary artery disease than those with ε3/ε3, while those with ε2 are in low risk (with the exception of hypertiglyceremai Ⅲ). The aim of this study is to analyze in patients with ischemic heart disease the role of aplipoprotein E alleles in order to seek its correlation with coronary artery disease, as well as to seek whether the polymorphism of apo E produces any differences in the severity of coronary artery disease according to plasma lipid levels. Methods: The subjects for study were 273 patients admitted to the Internal Cardiology Division of the Soonchunhyang University Hospital form December 1998 to February 1999. The subjects were divided into the two groups of which one was ischemic heart disease (IHD) experiment group totaling 105 (avg.60.1 years of age, male/female ratio = 69/36) and the control group totaling 168 (avg. 59.7 years of age, male/female = 73:95). The coronary angiogram was given to 127 subjects, and of this total, 94 have developed significant stenosis in the coronary artery. The stages of the analyzing of the apo E phenotype was first, the separation of DNA from the blood samples, subjecting it to the PCR from with 228 base pairs of expanded products were obtained. The band was determined by means of the reverse hybridization principle on the nitrocellulose strip. Results: From the 105 patients the distributions of apo E phenotypes were as follows: ε3/2(5.7%), ε4/2(1.9%), ε3/3(70%), ε4/3(20%), ε4/4(1.9%). The relative frequencies of each allele are as follow: ε2 (0.038), ε3 (0.833), ε4 (0.128). The results show as follows: ⅰ) The IHD experiment group to have a higher occurrence of ε4/3 phenotypes as well as ε4 alleles than the control group. ⅱ) Both the control group and IHD group showed the largest distribution of ε3/3 for phenotypes, and ε4 for alleles. ⅲ) The IHD group showed less ε2/3 phenotypes as well as significantly less allele frequency of ε3 in comparison to the control group. ⅳ) the IHD group showed a much lower level of HDL in comparison to the control group, while the LDL was significantly higher; samples including the apo ε2 showed a significantly higher level of HDL than those without. Among the control group, samples including apo ε2 showed a significantly higher level of TG (triglyceride) than samples without. No significant difference was found between the experiment apo ε4 sample and the control plasma lipid sample. ⅴ) No significant correlation was found between an apo E polynorphism and the number of involved arteries of a coronary angiogram. Conclusion: Between the experiment IHD group and control group were found differences in the frequency of alleles. The polymorphism of apo E alleles may contribute as a risk factor to the development of heart disease by involving itself in the metabolism and modulation of plasma lipids.

골프선수와 일반인의 견관절 등속성 근력에 관한 비교 연구

박영균,곽노석,최현희 경희대학교 체육과학연구소 2000 體育學論文集 Vol.28 No.-

The purpose of this study was to examine whether and how much isokinetic strength in shoulder is different between general male with age of earlier 20's who have not been experienced in golf and golfers who have been experienced for at least 5 years. For the purpose of this study, an intensive isokinetic test was carried out with thirteen general male and fourteen golfers by using Cybex 770. The test was conducted at two speeds of 60deg/sec (5 repetitions) and 180deg/sec (5 repetitions) for flexion and extension in left and right shoulders. From the above study, the following results were obtained: (1) Peak torque was significantly different (p〈0.001) between general male and golfers for flexion and extension at all of the above speeds. (2) The ratio of peak torque to body weight was significantly different (p〈0.001) for extension and also different (p〈0.01) for flexion at all of the speeds. (3) Peak torque ratio of flexion to extension was significantly different (p〈0.01) with the exception of left shoulder at a speed of 60deg/sec. (4) Average power was significantly different (p〈0.001) for flexition and extension at all of the above speeds. (5) Average power to body weight was significantly different (p〈0.001) with the exception that it was significantly different (p〈0.05) only for flexion at a speed of 60deg/sec.

염지 중 베이컨육의 특성 변화

문영덕,문윤희,곽석범 慶星大學校 1989 論文集 Vol.10 No.2

Cured becon meats were manufactured from bacon using dry curing method with varing levels of sodium chloride. To study the changes of moisture content, water activity, pH, salt concentration, extractability of proteins, SDS-PAGE and ATPase activity of protein were investigated during curing period at 3±1℃. During the process of curing, the moisture content and water activity in bacon meat decreased while the pH and salt concentration increased. Extractabilities of protein and SDS-PAGE shows that changes in extractability of water soluble protein are less noticeable while the extractability of salt soluble protein depended on curing period. These changes were rapid in the high level concentration of sodium chloride. The ATPase activity of myofibril from cured bacon with low level of salt concentration demonstrated a higher sensitivity to ionic strength than that of myofibril from cured bacon with high levels.

대전대학교 기숙사 식당의 급식만족도 조사

임영희,김미원,곽현옥,박상현 대전대학교 생활과학연구소 2002 생활과학연구 Vol.8 No.-

This study aimed at examining the foodservice satisfaction on dormitory restaurant by gender, age, college. The subjects of the study were 236 students living in the dormitory of Daejeon University. The survey was conducted between June 4 to June 12, 2002. The data was collected by survey and- was analyzed by SPSS package. The evaluation of the foodservice satisfaction by gender, showed that answers of the most students were below average. Generally, male students were more satisfied than females. The evaluation of the foodservice satisfaction by age, showed that younger students were more disatisfied than older students. The results suggest that food preference of subjects should be considered in meal planning and various cooking method should be utilized to enhance taste of foods for better foodservice. The management of the foodservice will be made efforts to improve the quality of foodservice on the basis of these results.