Enhanced Production of Carboxymethylcellulase by a Newly Isolated Marine Microorganism Bacillus atrophaeus LBH-18 Using Rice Bran, a Byproduct from the Rice Processing Industry

Yi-Joon Kim(김이준),Wa Cao(고와),이유정,Sang-Un Lee(이상운),정정한,Jin-Woo Lee(이진우) 한국생명과학회 2012 생명과학회지 Vol.22 No.10

Carboxymethylcellulase를 생산하는 미생물을 해수에서 분리하여 16S rDNA의 염기서열을 분석하고 계통 발생학 방법으로 비교한 결과, Bacillus atrophaeus로 확인되었다. 이 해양 미생물을 B. atrophaeus LBH-18로 명명하였으며 response surface method (RSM)를 사용하여 carboxymethylcellulase의 생산 조건을 최적화하였다. 이 균주의 생육에 최적인 미강, 펩톤 및 배지의 초기 pH는 68.1 g/l, 9.1 g/l 및 7.0이었으나, carboxymethylcellulase의 생산에 최적인 조건은 각각 55.2 g/l, 6.6 g/l 및 7.1이었다. 이 균주의 생육과 carboxymethylcellulase의 생산에 최적인 온도는 30℃이었다. 이 균주의 생육에 최적인 생물배양기의 교반속도 및 통기량은 324 rpm 및 0.9 vvm이었으나, carboxymethylcellulase의 생산에 최적인 조건은 각각 343 rpm 및 0.6 vvm이었다. 파이롯트 규모의 생물배양기를 사용하여 실험한 결과, 이 균주의 생육과 carboxymethylcellulase의 생산에 최적인 내압은 0.06 MPa이었다. 최적 조건의 내압으로 배양한 결과, 이 균주의 carboxymethylcellulase의 생산성은 127.5 U/ml이었으며, 이 결과는 내압을 가하지 않고 배양한 경우에 비하여 1.32배 향상된 것이다. 본 연구를 통하여 쌀 도정 공정의 부산 물인 미강을 기질로 개발하였으며 해양 미생물을 사용하여 carboxymethylcellulase의 생산기간을 7~10일에서 3일로 단축시켰다. A microorganism producing carboxymethylcellulase (CMCase) was isolated from seawater and identified as Bacillus atrophaeus. This species was designated as B. atrophaeus LBH-18 based on its evolutionary distance and the phylogenetic tree resulting from 16S rDNA sequencing and the neighbor-joining method. The optimal conditions for rice bran (68.1 g/l), peptone (9.1 g/l), and initial pH (7.0) of the medium for cell growth was determined by Design Expert Software based on the response surface method; conditions for production of CMCase were 55.2 g/l, 6.6 g/l, and 7.1, respectively. The optimal temperature for cell growth and the production of CMCase by B. atrophaeus LBH-18 was 30℃. The optimal conditions of agitation speed and aeration rate for cell growth in a 7-l bioreactor were 324 rpm and 0.9 vvm, respectively, whereas those for production of CMCase were 343 rpm and 0.6 vvm, respectively. The optimal inner pressure for cell growth and production of CMCase in a 100-l bioreactor was 0.06 MPa. Maximal production of CMCase under optimal conditions in a 100-l bioreactor was 127.5 U/ml, which was 1.32 times higher than that without an inner pressure. In this study, rice bran was developed as a carbon source for industrial scale production of CMCase by B. atrophaeus LBH-18. Reduced time for the production of CMCase from 7 to 10 days to 3 days by using a bacterial strain with submerged fermentation also resulted in increased productivity of CMCase and a decrease in its production cost.

Pilot-scale Optimization of Parameters Related to Dissolved Oxygen for Mass Production of Pullulan by Aureobasidium pullulans HP-2001

Wa Gao(고와),Yi-Joon Kim(김이준),Chung-Han Chung(정정한),Jianhong Li(이잔홍),Jin-Woo Lee(이진우) 한국생명과학회 2010 생명과학회지 Vol.20 No.10

Aureobasidium pullulans HP-2001 균주를 사용하여 풀루란을 대량 생산을 위하여 7 l 및 100 l 생물배양기를 사용하여 용존산소와 관련된 조건을 최적화하였다. 풀루란의 생산에 최적인 탄소원과 질소원은 각각 50.0 g/l 포도당 및 2.5 g/l 효모추출물이었으며 플라스크 규모에서의 풀루란 변환율은 37%이었다. 풀루란 생산 균주의 생장에 최적인 배지의 초기 pH 및 배양온도는 7.5 및 30℃이었으나 풀루란의 생산에 최적인 배지의 초기 pH 및 배양 온도는 각각 6.0 및 25℃이었다. 7 l 생물배양기에서 Aureobasidium pullulans HP-2001 균주의 생육에 최적인 교반속도 및 통기량은 각각 600 rpm 및 2.0 vvm이었으나 풀루란 생산에 최적인 조건은 각각 500 rpm 및 1.0 vvm이었으며 최적 조건에서 풀루란의 생산농도는 18.13 g/l이었다. 100 l 생물배양기에서 풀루란 생산 균주의 생장에 최적인 내압은 0.0 ㎏f/㎠이었으나, 풀루란 생산에 최적인 내압은 0.4 ㎏f/㎠이었으며 최적 조건에서 풀루란의 생산 농도는 22.89 g/l이었다. 이는 내압이 없는 상태에 비하여 풀루란의 생산 농도가 1.38배 증가한 것이다. Parameters related to dissolved oxygen for the production of pullulan by Aureobasidium pullulans HP-2001 were optimized in 7 l and 100 l bioreactors. The optimal concentrations of glucose and yeast extract for the production of pullulan were 50.0 and 2.5 g/l, respectively, and its conversion rate from glucose was 37% at a flask scale. The optimal initial pH of the medium and temperature for cell growth were 7.5 and 30℃, whereas those for the production of pullulan were 6.0 and 25℃. The optimal agitation speed and aeration rate for cell growth were 600 rpm and 2.0 vvm in a 7 l bioreactor, whereas those for the production of pullulan were 500 rpm and 1.0 vvm. The production of pullulan with an optimized agitation speed of 500 rpm and aeration rate of 1.0 vvm was 18.13 g/l in a 7 l bioreactor. Maximal cell growth occurred without inner pressure, whereas the optimal inner pressure for the production of pullulan was 0.4 ㎏f/㎠ in a 100 l bioreactor. The production of pullulan under optimized conditions in this study was 22.89 g/l in a 100 l bioreactor, which was 1.38 times higher than that without inner pressure.

안구건조증 모델에서 미세조류의 완화 효과

김진우(Jin-Woo Kim),김이준(Yi-joon Kim),김성진(Sung Jin Kim),김진철(Jin-Chul Kim) 한국식품과학회 2023 한국식품과학회지 Vol.55 No.5

Biosorption of Metal Ions by Seaweed Alginate, Polyguluronate, and Polymannuronate

Dae-Young Jung(정대영),Chang-Woo Son(손창우),Sung-Koo Kim(김성구),Yi-Joon Kim(김이준),Chung-Han Chung(정정한),Jin-Woo Lee(이진우) 한국생명과학회 2009 생명과학회지 Vol.19 No.5

P1/2 값을 참고로 비교한 알긴산, 폴리글루론산 및 폴리만뉴론산의 금속이온들에 대한 상대적인 친화성은 다음과 같다; 1) 알긴산: Cu<SUP>2+</SUP>>Cd<SUP>2+</SUP>>Pb<SUP>2+</SUP>>Fe<SUP>3+</SUP>>>Zn<SUP>2+</SUP>>Sr<SUP>2+</SUP>>Ca<SUP>2+</SUP>>Co<SUP>2+</SUP>>>Cr<SUP>6+</SUP>>Mn<SUP>2+</SUP>>>Hg<SUP>2+</SUP>, Mg<SUP>2+</SUP>, Rb?, 2) 폴리글루론산: Cd<SUP>2+</SUP>>Cu<SUP>2+</SUP>>Pb<SUP>2+</SUP>>Fe<SUP>3+</SUP>>>Ca<SUP>2+</SUP>>Sr<SUP>2+</SUP>, Zn<SUP>2+</SUP>, Co<SUP>2+</SUP>>>Mn<SUP>2+</SUP>>Cr<SUP>6+</SUP>>>Hg<SUP>2+</SUP>, Mg<SUP>2+</SUP>, Rb?, 그리고 3) 폴리만뉴론산: Cd<SUP>2+</SUP>, Cu<SUP>2+</SUP>>Fe<SUP>3+</SUP>>Pb<SUP>2+</SUP>>Ca<SUP>2+</SUP>>Zn<SUP>2+</SUP>>Sr<SUP>2+</SUP>>Co<SUP>2+</SUP>>Cr<SUP>6+</SUP>>>Mn<SUP>2+</SUP>>>Hg<SUP>2+</SUP>, Mg<SUP>2+</SUP>, Rb?. 알기산 1 g에 흡착하는 Cd<SUP>2+</SUP>, Cu<SUP>2+</SUP>, Fe<SUP>3+</SUP>, Pb<SUP>2+</SUP> 및 Zn<SUP>2+</SUP>의 양은 363.5±45.0, 226.3±9.2, 1,299.4±81.3, 500.7±27.7 및 165.9±11.4 ㎎이었으며, 폴리글루론산 1g에 흡착하는 Cd<SUP>2+</SUP>, Cu<SUP>2+</SUP>, Fe<SUP>3+</SUP>, Pb<SUP>2+</SUP> 및 Zn<SUP>2+</SUP>의 양은 354.5±26.5, 177.6±8.7, 1,288.6±60.1, 424.0±7.4 및 140.2±28.5 ㎎이었으나, 폴리만뉴론산 1 g에 흡착하는 Cd<SUP>2+</SUP>, Cu<SUP>2+</SUP>, Fe<SUP>3+</SUP>, Pb<SUP>2+</SUP> 및 Zn<SUP>2+</SUP>의 양은 329.0±10.3, 226.9±1.9, 1,635.6±11.1, 419.8±12.6 및 251.0±49.1 ㎎이었다. 폴리만뉴론산은 알긴산보다 높은 용해도와 폴리글루론산보다 높은 금속이온에 대한 친화성 때문에 독성이 높은 중금속이나 경제성이 높은 금속을 선택적으로 분리하는 데 사용할 수 있을 것이다. Based on P1/2 values, relative affinities of alginate, polyguluronate, and polymannuronate for metal ions are, in order, as follows; 1) seaweed alginate: Cu<SUP>2+</SUP> > Cd<SUP>2+</SUP> > Pb<SUP>2+</SUP> > Fe<SUP>3+</SUP> >> Zn<SUP>2+</SUP> > Sr<SUP>2+</SUP> > Ca<SUP>2+</SUP> > Co<SUP>2+</SUP> >> Cr<SUP>6+</SUP> > Mn<SUP>2+</SUP> >> Hg<SUP>2+</SUP>, Mg<SUP>2+</SUP>, Rb?, 2) polyguluronate: Cd<SUP>2+</SUP> > Cu<SUP>2+</SUP> > Pb<SUP>2+</SUP> > Fe<SUP>3+</SUP> >> Ca<SUP>2+</SUP> > Sr<SUP>2+</SUP>, Zn<SUP>2+</SUP>, Co<SUP>2+</SUP> >> Mn<SUP>2+</SUP> > Cr<SUP>6+</SUP> >> Hg<SUP>2+</SUP>, Mg<SUP>2+</SUP>, Rb? , and 3) polymannuronate: Cd<SUP>2+</SUP>, Cu<SUP>2+</SUP> > Fe<SUP>3+</SUP> > Pb<SUP>2+</SUP> > Ca<SUP>2+</SUP> > Zn<SUP>2+</SUP> > Sr<SUP>2+</SUP> > Co<SUP>2+</SUP> > Cr<SUP>6+</SUP> >> Mn<SUP>2+</SUP> >> Hg<SUP>2+</SUP>, Mg<SUP>2+</SUP>, Rb?. Amounts of the metal ions, Cd<SUP>2+</SUP>, Cu<SUP>2+</SUP>, Fe<SUP>3+</SUP>, Pb<SUP>2+</SUP>, and Zn<SUP>2+</SUP>, bound to 1 g of seaweed alginate, were measured as 363.5±45.0, 226.3±9.2, 1,299.4±81.3, 500.7±27.7, and 165.9±11.4 ㎎, respectively. Amounts of the metal ions, Cd2+, Cu2+, Fe3+, Pb2+, and Zn2+, bound to 1g of polyguluronate, were 354.5±26.5, 177.6±8.7, 1,288.6±60.1, 424.0±7.4, and 140.2±28.5 ㎎, respectively, whereas those bound to 1 g of polymannuronate were 329.0±10.3, 206.9±1.9, 1,635.6±11.1, 419.8±12.6, and 251.0±49.1 ㎎, respectively. Due to its higher solubility than alginate and higher affinity for metal ions than polyguluronate, polymannuronate can be used for bioremediation or biosorption of toxic and/or noble metal ions.

지연제를 혼입한 속경성 콘크리트의 지연성능 및 강도 평가

이겨레(Lee, Kyeo Re),윤경구(Yun, Kyong Ku),김이준(Kim, Yi Joon),백윤식(Baek, Yoon Sik),최성용(Choi, Sung Yong) 대한토목학회 2017 대한토목학회 학술대회 Vol.2017 No.10

셀룰러 스프레이 콘크리트의 공정 별 슬럼프 변화

한승연(Ham, Seung Yeon),윤경구(Yun, Kyong Ku),김이준(Kim, Yi Joon) 한국콘크리트학회 2017 한국콘크리트학회 학술대회 논문집 Vol.29 No.1