Treatment of Dyrk1A-dependent Mental Retardation of Down Syndrome: Isolation of Human Dyrk1A-specific shRNA

Min-Su Jung(정민수),Yeun-Soo Kim(김연수),Juhyun Kim(김주현),Joung-Hun Kim(김정훈),Sul-Hee Chung(정설희),Woo-Joo Song(송우주) 한국생명과학회 2009 생명과학회지 Vol.19 No.3

다운증후군은 추가적으로 존재하는 인간염색체 21번에 위치한 유전자의 과발현으로 발병한다. 다운증후군 환자에서 보이는 여러 증상들 중 학습과 기억능력 저하와 같은 뇌기능 저하는 다운증후군 환자가 독립적인 생활을 영위하는데 가장 큰 걸림돌이 된다. 인간염색체 21번에 위치하는 Dyrk1A는 신경발달에 중요한 역할을 하는 단백질로 Dyrk1A를 과발현 하는 형질전환 생쥐에서 심각한 해마 의존적 학습과 기억 장애가 보고되었다. 본 연구에서는 인간 Dyrk1A를 과발현 하는 형질전환 생쥐와 RNA interference (RNAi) 방법을 이용하여 endogenous mouse Dyrk1A의 발현은 정상적으로 유지하면서 exogenous human Dyrk1A 발현은 특이적으로 저해함으로써 인간 Dyrk1A 과발현에 의한 학습과 기억 능력저하를 회복시킬 수 있는지 동물모델에서 검증하기 위한 첫 단계로 인간 Dyrk1A 특이적 lentiviral short hairpin RNA (shRNA)를 발굴하였다. 발굴된 shRNA를 이용한 형질전환 모델생쥐에서의 증상의 회복 가능성 검증은 다운증후군의 뇌기능저하 치료제 개발에 중요한 정보를 제공할 것이다. Down syndrome (DS) results from overexpressed genes on an extra copy of human chromosome 21. Among various phenotypes seen in DS patients, mental retardation, such as learning and memory deficits, is a major factor that prevents DS individuals from leading fully independent lives. The Dyrk1A gene that plays a critical role in neurodevelopment has been isolated from chromosome 21, and transgenic mice with over-expression of Dyrk1A show severe hippocampal dependent learning and memory defects. In the present study, as an initial step to test the treatment of Dyrk1A dependent mental retardation phenotypes in model animals, we isolated human Dyrk1A specific lentiviral short hairpin RNA (shRNA) that inhibits the exogenous human Dyrk1A expression, but not the endogenous mouse expression in transgenic mice with human Dyrk1A overexpression. This limited and specific repression of exogenous human Dyrk1A will prove to be valuable information, if Dyrk1A dependent learning and memory defects in DS patients could be treated or at least ameliorated in vivo.

탄소섬유적층 각도에 따른 CFRP 평판의 실내소음 특성

방성일(Sungil Bang),김연수(Yeun Soo Kim),백지선(Jiseon Back),이상권(Sang-Kwon Lee) 한국소음진동공학회 2018 한국소음진동공학회 논문집 Vol.28 No.5

어머니의 운동이 여대생 딸의 운동, 비만지표, 사회심리적 건강지수 및 영양소 섭취에 미치는 영향

조강옥(Cho Kang Ok),김연수(Kim Yeun Soo) 한국지역사회생활과학회 2008 한국지역사회생활과학회지 Vol.19 No.2

한국인 간세포암 환자에서 HBsAg 및 anti - HCV 의 발현상

김주현(Ju Hyun Kim),김연수(Yeun Soo Kim),서동진(Dong Jin Suh) 대한내과학회 1994 대한내과학회지 Vol.46 No.2

N/A Obifectives: Hepatocellular carcinoma (HCC) is one of the most common malignant neoplasms. Epidemiologic surveys have established that hepatitis B virus (HCV) is a major etiologic factor. It has 1ong been suspected that non-A, non-B (NANB) hepatitis infection may occasionally lead to HCC, and the development of an assay for antibodies against hepatitis C virus (anti-HCV) has confirmed that HCV is frequently involved in chronic liver disease and HCC. In western countries and Japan, the prevalence of anti-HCV in patients with HCC was higher than that of HBsAg, which suggested that HCV infection plays a more important etiologic role in the development of HCC. But in South Africa and Taiwan where the prevslence of HBsAg is high, HBsAg was detected more frequently than anti-HCV in patients with HCC. We investigated the prevalence of HBsAg and anti-HCV in 427 patients with HCC to evaluate etiologic role of HBV and HCV in HCC in Korea where HBV infection is endemic. Methods: The sera which had been collected on admission and stored at -20℃ were tested for HBsAg, HBeAg and AFP, using commercially available RIA kits (Ausria-II, Abbott-HBe and alpha-Feto RIABEAD; Abbott Lab., North Chicago, IL). Anti-HCV was detected by the 2nd generation ELA kit (United Biochemical Inc.). Results: 1) In 427 patients with HCC, 306 cases (71.7%) were positive for HBsAg. 2) Anti-HCV was positive in 20.4% (87/427) of patients with HCC. The positive rate of anti-HCV was significantly higher in HBsAg-oegative patients (42.1%) than in HBsAg-positive ones (11.8%). 3) Considering HBsAg and anti-HCV positivity together, 63.2% of patients with HCC were positive for HBsAg only, 12.0% positive for anti-HCV only and 8.4% positive for both HBsAg and anti-HCV, 16.4% of patients had neither HBsAg nor anti-HCV. 4) Male to female sex ratio of patients with HCC was 4:1 as a whole, and highest prevalence was found in the fifties. However, patients positive for anti-HCV only had relative female preponderance (M:F=2.6:1) than HBsAg-positive patients and highest prevalence was found in the sixties in them. 5) In patients positive for both HBsAg and anti-HCV, 67.6% had serum AFP level greater than 400 ng/ml, while those with neither HBsAg nor anti-HCV tended to have lower AFP and 47.1% had serum AFP level less than 100 ng/ml. Conclusion: It is suggested that HBV is the main etiologic factor of HCC in Korea. However, HCV infection is not uncommon, especially in HRsAg-negative HCC and HCV is considered another risk factor for HCC. HCC patients positive for anti-HCV only tend to be older in age and to have relative female preponderance and lower serum AFP level than HBsAg-positive patients.

신경회로망과 HMM을 이용한 한국어 인식에 관한 연구

이승용(Seung Young Lee),김연수(Yeun soo Kim),유병욱(Byong wook Yoo),김창석(Chang seok Kim) 한국통신학회 1992 한국통신학회 학술대회논문집 Vol.1992 No.7

Neural-Fuzzy System을 이용한 한국어 단어인식에 관한 연구

김연수,김창석 明知大學校 産業技術硏究所 1992 産業技術硏究所論文集 Vol.11 No.-

This dissertation aims about Korean-word recognition using the neural networks and fuzzy inference system (NFS), which is absorbing fluctuation due to personal difference in order to develop recognition rate. This method regards propability index observation each word model as input parameters of neural networks, and recognizes input words using fuzzy ingerence with membership function with is consisted with output of neural networks. Word recognition using fuzzy inference to develop recognition rate, which is absorbing flucturation due to personal difference, speaker jindependent word recognition system of a cause false-recongition. To evaluate validity of this method, this experiment has been carried out female and males speech data that is made 28DDO local area names. As a result of testing HMM (DHMM) with 8 state, condeword is 64, the recongition rate 91[%] , as a result of testing neural network with 64 codeword the recognition rate is 89 [%]. Finally, as a result of testing NFS with 64 codeword which is in the best condition in former tests, the recongition rate is 96 [%]

르 꼬르뷔제의 주택사상과 작품분석

김연수,배윤선,주혜련 연세대학교 생활과학대학 1992 婦學 Vol.25 No.-

기저세포암 153예의 임상적 고찰

김경호,이증훈,서기범,박장규,김형주,김연수 대한피부과학회 1999 대한피부과학회지 Vol.37 No.11

Background:Clinical features of basal cell epithelioma(BCE) are known to be different according to social and environmental factors. Recently, there have been reports of an increase of BCE in the Chung-Nam area. Objective:We evaluated the incidence of tumors, age and sex distribution, and tumor sites. Methods:Clinical records were reviewed for 153 cases of BCE with a histologically confirmed diagnosis for patients who visited the Department of Dermatology, Chungnam National University Hospital during the 20 years from 1978 to 1997. Pathologic characteristics were also reviewed. Results:The frequency of BCE expressed as a percentage of the total number of new patients increased from 0.10% in 1978 to 0.76% in 1997. The ratio of man to woman with BCE was 1:1.1 with a mean age of 61.8 years. Most cases of BCE were on the face (90.8%), especially on the nose (29%). The solid type (66.7%) was the most common pathologic pattern, followed by the adenoid type (9.7%), and the morpheic type (8.6%). The pigmented type of BCE accounted for 41.9%. Conclusion:The incidence of BCE has increased during the past 20 years. However, the age of occurrence, common site, and pathologic patterns did not show a significant difference from Caucasians, except for a higher frequency of pigmented type BCE, as can be expected in oriental people.

댄스스포츠 트레이닝이 여대생의 심폐기능과 신체구성 및 혈중 콜레스테롤에 미치는 영향

정진욱,전태원,김연수,김은경,김광준,이경영,박성태,전병환 한국운동과학회 2003 운동과학 Vol.12 No.1

정성태, 전태원, 정영수, 이동기, 박익렬, 엄우섭, 우재홍, 김경래. 대도시 직장인의 체력과 건강인식에 대한 연구. 운동과학, 제12권 제1호, 133-152, 2003. 본 연구의 목적은 대도시 직장인의 체력과 건강인식의 관계를 규명하는 데 있다. 연구대상자는 서울 지역에서 3개구를 임의 선정하여 각 구별로 3개의 단체를 무선 표집하여 연령별로 30-50대의 남·여 130명을 선정한 후, 이들을 대상으로 건강인식에 대한 설문조사와 건강관련 기초체력검사를 실시하였다. 자료처리 방법은 Crosstab을 통하여 사회인구학적 변인과 각 문항간의 χ^2검정을 실시하였으며, 사회인구학적 변인별로 체력과 건강인식관련 하위 변인간의 유의도 검증은 독립 t-test를 사용하여 분석하였다. 체력과 건강인식에 대한 설문조사와 기초체력검사를 실시한 결과 다음과 같은 결과를 얻었다. 첫째, 남자가 여자에 비해 직장 취업률이 높은 것으로 나타났다. 둘째, 남자보다 여자가 체중감소 노력을 더 많이 하는 것으로 나타났다. 셋째, 체중감소 희망부위에 대해 남자는 허리인 반면에 여자는 복부인 것으로 나타났다. 넷째, 비반의 원인에 대해 남자의 경우 운동부족을, 여자의 경우 음식 불균형에 의해 비만이 주로 초래된다고 인식하는 것으로 나타났다. 다섯째, 체중조절 방법에 대해 30대의 경우 주로 운동요법에 의해, 50대의 경우 식이요법에 의해 체중을 조절하고 있는 것으로 나타났다. 여섯째, 남자는 대다수가 흡연을 하는 것으로, 여자는 대다수가 흡연을 하지 않는 것으로 나타났다. 일곱번째, 흡연기간에 대해 남자는 흡연기간이 주로 10년 이상으로 나타났으며, 반면에 여자 흡연자의경우 흡연기간은 1년 미만인 것으로 나타났다. 여덟째, 음주횟수는 남자의 경우 평균 주 3회, 여자의 경우 평균 주 1회 술을 마시는 것으로 나타났다. 아홉째, 음주량은 남자의 경우 평균 7-9병을, 여자의 경우 4병 정도를 마시는 것으로 나타났다. 열번째, 스트레스 원인에 대해 남자는 주로 직장문제로, 여자는 주로 경제적 문제 때문에 스트레스를 받는 것으로 나타났다. 열한번째, 스트레스 해소방법에 대해 고학력자인 경우 이완요법을, 고졸이하의 경우 주로 운동에 의해 스트레스를 해소하는 것으로 나타났다. 열두번째, 운동횟수에 대해 연령이 증가함에 따라 운동횟수가 감소하는 것으로 나타났다. 열세번째, 운동시간에 대해 남자는 주당 평균 2-4시간 정도를, 여자는 1시간에서 1시간 30분 정도를 운동하는 것으로 나타났다. 열네번째, 운동종목 선호도에 대해 남자는 주로 조깅 및 헬스를, 여자는 주로 헬스 및 수영을 주로 선호하는 것으로 나타났다. 열다섯번째, 여가시간 운동여부에 대해 남자는 여가시간에 주로 운동을 하는 것으로 나타났으며, 여자는 주로 운동을 하지 않은 것으로 나타났다. 열여섯번째, 남자가 여자에 비해 근력, 근지구력, 심폐지구력 등이 높은 것으로 나타났다. 반면에, 여자가 남자에 비해 유연성, 체지방율 등이 높은 것으로 나타났다. Chung, S.T., Jun, T.W., Chung, Y.S., Lee, D.K, Park, I.R., Eom, W.S., Woo, J.H., Kim, K.L The Study of Fitness and Health Consciousness for Big City Company Employee's. Exercise Science, 12(1): 133-152, 2003. The purpose of this study is to examine closely big city company employee's relationship between fitness and health consciousness. Research targets were 130 men and women in ages of 30-50 of 3 organizations that were chosen randomly in 3 districts (gu)'s of Seoul area and they took the survey about health consciousness, basic physical test. Ran χ^2 test between society-population factor and each questions by doing cross-tab method to get the statical analysis and used independent-test between society-population factor and other subordinate factor(about fitness and health consciousness) to see the significant level. Got the following results after taking the surveys and physical tests. First, man appeared that man has higher employment rate than woman does. Second, woman was more exposed to weight loss effort than man did. Third, man's weight loss hope region was waist while woman was abdomen area. Fourth, in man's case about cause of corpulence was exposed due to lack of exercise and imbalance of food in the case of woman. Fifth, weight control system was exercising in ages of 30 and food control by people in ages of 50. Sixth, most of the men were smokers but only a few women were smokers. Seventh, smoking period of man was more than 10 years mainly and while smoking period was less than 1 year in woman's case. Eighth, in average man usually drinks 3 times a week and 1 time a week in woman's case. Ninth, man drinks 7 - 9 bottles and woman drinks about 4 bottles in averages. Tenth, about stress cause, man appeared to be as company problems, that woman receives stress from economical problem mainly. Eleventh, people with high education levels did relaxation(이완??) method to treat stress but people's with less than high school levels got rid of stress by exercise. Twelfth, exercise number decreased as people gets older. Thirteenth, man's average exercise time was about 2 - 4 hours per week, and 60 - 90 minutes in a week for woman. Fourteenth, man prefers jogging and health-club exercise, and woman prefers also health-club exercises and swimming. Fifteenth, man exercises mainly at leisure times but woman usually does not exercise during spare time. Sixteenth, man has higher muscle strength, muscle endurance, cardio-respiratory function than woman has. However, woman has higher value on flexibility and % body fat than man has.

흰쥐 각 장기에서 Transglutaminase 1 발현에 관한 면역조직화학적 연구

이증훈,김수열,김원식,박장규,김연수,박영군,박경란 대한해부학회 1998 Anatomy & Cell Biology Vol.31 No.5