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- 저자 : Y.H. Jung(정용환) (검색결과 6 건)
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Oh,Y.S.,Kayano, Hiroshi,Jung,Y.H.,Oh,M.Y.,Hong,S.S.,Kim,S.J.,Kim,G.O.,Ko,M.H. 濟州大學校 基礎科學硏究所 1997 基礎科學硏究 Vol.10 No.2
네 개의 다른 말 집단(Cheju native horses I; Cheju Racing horses, Cheju native horses II; Chejudo Livestock Promotion Institute horses, Tsushima native horses, Thoroughbreds)에서 mitochondrial DNA(mtDNA)의 제한효소 단편양상을 연구하였다. 122개체의 total DNA는 말의 mtDNA probe를 사용하여 18개의 제한효소 (Bam HⅠ, EcoRⅠ, HindIll, A vall,HpaⅠ,BglⅡ, XbaⅠ,PstⅠ,pvuⅡ,SacⅠ, KpnI, XhoI, AvaI, HincII, Bst EII, NotI, AccI, and HaeII)에 대한 분석을 하였다. 네 집단에서 16개의 다른 단편양상(mtDNA types)을 조합한 결과 12개의 Morphs를 관찰하였다. 네 집단에서 12개의 restriction types를 갖고 UPGMA 방법에 의하여 계통수를 작성한 결과 2개의 clusters로 분산되었다. 첫 번째 cluster는 주로 1, 6, 3, 8, 9, 2 types, 두 번째 cluster는 4, 7, 10, 11, 12 types들로 각각 구성되었다. 쓰시마재래마는 mtDNA types의 동질성이 높은 빈도를 보였다. 제한 효소 단편양상에서 제주재래마 두 집단과 Thoroughbreds 집단은 polymorphic한 반면, 쓰시마재래마는 monomorphic(1-2-1-1 mtDNA type)함을 보였다. 제주재래마집단 II와 쓰시마재래마가 진화학적 유연관계가 가장 가깝게 나타났으며, 두 집단간에 유전적거리는 1.5×10-3으로 추정되었다. Mitochondrial DNA(mtDNA) restriction endonuclease fragment patterns were studied in four different equine populations(Cheju native horses I; Cheju Racing horses, Cheju native horses II; Chejudo Livestock Promotion Institute horses, Tsushima native horses, Thoroughbreds). Total DNA samples from 122 individuals were analysed with 18 restriction enzymes, Bam HI, EcoRI, HindlⅡ,AvaⅡ,HpaⅠ, BglⅡ,XbaⅠ,PstⅠ,PvuⅡ,SacⅠ, KpnⅠ, XhoI, AvaI, HincII, BstEII, NotI, AccI, and HaeII using the horse mtDNA probe. Twelve morphs were observed yielding sixteen different combinations of fragment patterns(mtDNA types) in four populations. The phylogenetic tree constructed from the 12 restriction types by the UPGMA method indicated that mtDNA types in four populations were divided into two clusters. In this tree, the clear separation into the two clusters was observed, namely one with types 1, 6, 3, 8, 9, 2, and 5, and the other one with types 4, 7, 10, 11, and 12. Tsushima native horses were found to be highly homogeneous by the frequencies of mtDNA types. The populations of Cheju native horses I, II, and Thoroughbreds showed polymorphism, while the population of Tsushima native horses was monomorpic(only 1-2-1-1 type), acording to the restriction enzymes. Cheju native horses II were shown to be closely related to the Tsushima native horses, and the genetic distance between these two populations was 1.5×10-3.
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돼지 Melanocortin Receptor 1(MC1R) 대립유전자 3의 신규 유전변이 탐색
조인철,정용환,정진관,성필남,오운용,고문석,김병우,이정규,전진태 한국동물자원과학회 2004 한국축산학회지 Vol.46 No.1
This study was conducted to investigate novel genetic variations of MCIR/R*3 allele. In general, white spotting or white belt on a black backgroud in pigs is determined by the E^(p) allele at the MICR/Extention locus. E^(p) shares a frameshift mutation with the E^(p) allele for dominant black color. An oligonucleotide primer set was designed to amplify complete coding sequence of the porcine MICR gene. The MICR coding sequences obtained from five breeds those were Landrace(white), Yorkshire(white), Hampshire(belt), Berkshire(spot) and Jeju native black pigs(black), were used for this study. A multiple sequence alignment of the MICR coding region using Clustal W was performed. The total length of the MCIR coding sequence ranged from 963 to 966 base pairs(bp) among the selected breeds. The sequence analysis of the complete coding region of MICR was revealed that Hampshire and Jeju native black pig have 3 cytosines deletion and Birkshire has 2 cytosines deletion at codon 23(nt68) in Extention loci. Besides the finding, there were three different missense mutations and a frameshift mutation in the MCIR coding region.
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Single Stranded Conformation Polymorphism 분석에 의한 돼지 Duroc 품종의 미토콘드리아 DNA 유전적 변이
조인철,정용환,정진관,성필남,김병우,이정규,전진태 한국동물자원과학회 2003 한국축산학회지 Vol.45 No.6
The mitochondrial DNA(mtDNA) D-loop region was amplified from Duroc(Su scrofa) by polymerase chain reaction(PCR). The oligonucleotide primer used to amplify the Sus scrofa mtDNA D-loop region was designed using tRNA-Pro and tRNA-Phe sequence in mtDNA regions highly conserved in many other amimal species. There were 1,145 base paris(bp) in the D-loop region. the middle of the region contained 10 tandem repeat of an 10-bp Sus scrofa-specifie sequence, TACACGTGCG. We designed primers for PCR-mediated single stranded conformation polymorphism(SSCP) analysis that amplified of denatured amplification products was carried out by polyacrylamide(8%) gel electrophoresis followed by ethidium bromide staining. The SSCP analysis identified two band patterns(A and B) and comparision of these two nucleotide sequences identified 21 base substitutions. These results show that SSCP analysis of the D-loop region is useful for detecting the genetic polvmorphism.
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제주재래미와 쓰시마재래마에서 미토콘드리아 DNA 의 제한효소 분석과 진화학적 유연관계
오문유,고미희,김기옥,오유성,정용환,김세재,홍성수,모야박 한국축산학회 1997 한국축산학회지 Vol.39 No.4
Mitochondrial DNA(mtDNA) restriction endonuclease fragment patterns were studied in four different equine populations(Cheju native horses I; Cheju Racing horses, Cheju native horses II; Chejudo Livestock Promotion Institute horses, Tsushima native horses, Thoroughbreds). Total DNA samples from 122 individuals were analyzed with 18 restriction enzymes, Bam HI, EcoRI, HindIII, AvaII, HpaI, BgIII, XbaI, PstI, Pvu II, Sac I, Kpn I, Xho I, AvaI, Hinc II, Bst EII, Not I, Acc I, and Hae II using the horse mtDNA probe. Twelve morphs were observed yielding sixteen different combinations of fragment patterns(mtDNA types) in four populations. The phylogenetic tree constructed from the 12 restriction types by the UPGMA method indicated that mtDNA types in four populations were divided into two clusters. In this tree, the clear separation into the two clusters was observed, namely one with types 1, 6, 3, 8, 9, 2, and 5, and the other one with types 4, 7, 10, 11, and 12. Tsushima native horses were found to be highly homogeneous by the frequencies of mtDNA types. The populations of Cheju native horses I, II, and Thoroughbreds showed polymorphism, while the population of Tsushima native horses was monomorpic(only 1-2-1-1 type), according to the restriction enzymes. Cheju native horses II were shown to be closely related to the Tsushima native horses, and the genetic distance between these two populations was 1.5×10^(-3).
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