http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
지칭개(Hemisteptia lyrata) 꽃의 성분연구(I)
하태정,이경동,이종록,이준,박기훈,양민석,Ha, Tae-Joung,Lee, Kyung-Dong,Lee, Jong-Rok,Lee, Jun,Park, Ki-Hun,Yang, Min-Suk 한국생약학회 2001 생약학회지 Vol.32 No.3
The leaves and flowers of Hemisteptia lyrata Bunge (Compositae) have been used for cure sour throat and treat tumors in traditional chinese medicine. As a result of the chemical investigation, two compounds were isolated from the $CHCl_3$ extract of the flowers of H. lyrata. On the basis of spectroscopic experiments, their structures were identified as 8-hydroxy-3-keto-10(14)-guaien-12,6-oxide (Isoamberboin) and 3,8-dihydroxy-4(15),10(14),11(13)-guaiatrien-12,6-oxide (8-Hydroxyzaluzanin C). They were first isolated from the genus Hemisteptia.
하태정,이병원,이종록,이준,황선우,조동령,남상해,양민석,이경동,Ha, Tae-Joung,Lee, Byong-Won,Lee, Jong-Rok,Lee, Jun,Hwang, Sun-Woo,Cho, Dong-Young,Nam, Sang-Hae,Yang, Min-Suk,Lee, Kyung-Dong 한국응용생명화학회 2003 한국농화학회지 Vol.46 No.1
쑥갓(Chrysanthemum coronarium L.) 잎으로부터 생리활성 성분을 가진 3풀의 화합물을 분리하여 다양한 spectral data를 종합한 결과, 화합물 1, 2, 3은 terpenoid의 일종인 monoterpene lactone, sesequiterpene lactone과 sterol 화합물인 것으로 밝혀졌으며, 이들은 각각 dihydrotulipinolide, loliolide, $5{\alpha},8{\alpha}-peroxyergosterol$로 동정되었다. 이 화합물들은 쑥갓에서 처음으로 분리-보고되는 화합물이다. 분리된 화합물들의 세포독성을 측정한 결과, 화합물 3은 3종의 인체암 세포주에 대하여 비교적 강한 활성을 나타내었다. Three terpenoids were isolated from the leaves of Chrysanthemum coronarium L. by silica gel chromatography and recrystalization. The structure of compounds 1, 2, and 3, using various spectroscopic data, were identified as to be terpenoid derivatives of dihydrotulipinolide, loliolide, and $5{\alpha},8{\alpha}-peroxyergosterol$, respectively. The presence of these 3 is the first time reported in this plant. The cytotoxic activity of $5{\alpha},8{\alpha}-peroxyergosterol$ showed strong activities against human cancer cell lines such as A549, PC-3, and HCT 116.
고착협 내탈립 기계수확 적응 장류⋅두부용 콩 품종 ‘새금’
김현태(Hyun Tae Kim),한원영(Won Young Han),이병원(Byung Won Lee),고종민(Jong Min Ko),이영훈(Yeong Hoon Lee),백인열(In Youl Baek),윤홍태(Hong Tai Yun),하태정(Tae Joung Ha),최만수(Man Soo Choi),강범규(Beom Kyu Kang),김현영(Hyun Yeong K 한국육종학회 2019 한국육종학회지 Vol.51 No.4
The soybean cultivar, ‘Saegeum’, has been developed for preparing soy-paste and tofu. The soybean cultivars ‘Daepung’ and ‘SS98207-3SSD-168’ were crossed in 2003 to obtain ‘Saegeum’. Single seed descent method was used to advance the generation from F3 to F5, and the plant lines with promising traits were selected from F6 to F7 by pedigree method. The preliminary yield (PYT) and advanced yield trials (AYT) were conducted from 2009 to 2010, and the regional yield trial (RYT) was conducted in 12 regions between 2011 and 2013. The morphological characteristics of ‘Saegeum’ were as follows: determinate plant type, white flower, tawny pubescence color, and brown pod color. Flowering and maturity dates were August 2, XXXX and October 17, XXXX, respectively. Plant height, first pod height, number of nodes, number of branches, and number of pods were 79 cm, 18 cm, 16, 2.3, and 44, respectively. The seed characteristics of ‘Saegeum’ were as follows: yellow spherical shape, yellow hilum, and the 100-seed weight was 25.4 g. ‘Saegeum’ was resistant to bacterial pustule and SMV in the field test, and its lodging resistance was mildly strong, whereas its shattering resistance was excellent. The ability of this cultivar to be processed into tofu, soybean malt, and other fermented products was comparable with that of ‘Daewonkong’. The yield of ‘Saegeum’ in the adaptable regions was 3.02 ton ha-1. Thus, ‘Saegeum’ is adaptable to mechanized harvesting because of its high first pod height, as well as lodging and shattering resistance. (Registration number: 5929)
뽀리뱅이 전초로부터 Isoamberboin과 Isolipidiol의 분리
장대식,하태정,최상욱,남상해,박기훈,양민석,Jang, Dae-Sik,Ha, Tae-Joung,Choi, Sang-Uk,Nam, Sang-Hae,Park, Ki-Hun,Yang, Min-Suk 한국생약학회 2000 생약학회지 Vol.31 No.3
Two sesquiterpene lactones were isolated from methanol extracts of the whole plants of Youngia japonica (L.) DC. by the silica gel column chromatography and recrystallization. On the basis of spectrometric studies including $^1H-NMR,\;^{13}C-NMR,\;DEPT,\;^1H-^1H\;COSY,\;^{13}C-^1H\;COSY$, IR and MS, compounds 1 and 2 were identified as derivatives of 10(14)-guaien-12, 6-olide, isoamberboin and isolipidiol, respectively. This is the first report on the isolation of isoamberboin and isolipidiol from Youngia japonica (L.) DC.
권현숙,하태정,황선우,진영민,남상해,박기훈,양민석,Kwon, Hyun-Sook,Ha, Tae-Joung,Hwang, Seon-Woo,Jin, Young-Min,Nam, Sang-Hae,Park, Ki-Hun,Yang, Min-Suk Korean Society of Life Science 2006 생명과학회지 Vol.16 No.5
구절초 전초의 chloroform 분획물로부터 2종의 flavonoid화합물을 분리하여 NMR을 통해서 구조를 확인한 결과, luteolin (1)과 acacetin (2)으로 구조 동정되었다. 이들의 화합물 중에서 luteolin (1)은 구절초에서 처음으로 분리하였다. 분리된 화합물은 sulforhodamine B (SRB) assay법에 따라 인체암세포주인, HCT116 (결장암) , UO-31 (신장암), PC-3 (전립선암) 와 A549 (폐암)등에 대한 in vitro에서의 세포독성을 실험하였다. 그 결과, acacetin (2)이 HCT116 $(IC_{50}\;2.44\;{\mu}g/ml)$과 UO-31 $(IC_{50}\;2.89\;{\mu}g/ml)$에서 유의할만한 세포독성을 나타내었다. Two known flavonoids, luteolin (1) and acacetin (2) were isolated from a $CHCI_3$ soluble fraction of the whole plants of Chrysanthemum zawadskii Herbich var. latilobum Kitamura, and their structures were determined by NMR analysis. The luteolin (1) was isolated from this plant for the first time. These compounds were examined for their in vitro cytotoxic activities against four human cancer cell lines including HCT116 (colon), UO-31 (renal), PC-3 (prostate) and A549 (lung) by sulforhodamine B(SRB) assay. Acacetin (2) showed significant cytotoxic activity against HCT116 and UO-31 cells with an $IC_{50}$ of 2.44 and $2.89\;{\mu}g/ml$, respectively.
Inhibition of Matrix Metalloproteinase-2 Activity of Flavonol Glycosides from Cedreia sinensis
황선우,하태정,고영희,전효곤,이준,권현숙,박기훈,양민석,Hwang Seon-Woo,Ha Tae-Joung,Kho Yung-Hee,Chun Hyo-Kon,Lee Jun,Kwon Hyun-Sook,Park Ki-Hun,Yang Min-Suk Korean Society of Life Science 2006 생명과학회지 Vol.16 No.3
Cedrela sinensis is a broadleaf tree that is widely cultivated in Korea and China. It was used for treating enteritis, dysentery, and skin itch in oriental medicine. In this study, three major flavonoids, kaempferol-3-O-rhamnoside (1), quercetin-3-O-rhamnoside (2), and quercetin-3-O-glucoside (3), were isolated from the leaf of Cedrela sinensis. The biological activities of these compounds were tested by inhibitory activity of matrix metalloproteinases-2 (Type IV collagenase) method together with a cytotoxicity and a apoptosis test against human cancer cell lines. 참죽나무는 멀구슬과 나무로서 한국과 중국에 널리 분포하고 있다. 한방에서는 장염(腸炎), 이질(痢疾), 개선(疥癬) 등의 치료에 이용되고 있다. 본 연구는 참죽나무 잎에서 3종의 flavonol glycosides를 분리하여 NMR을 통해 구조를 확인한 결과, kaempferol-3-O-rhamnoside (1), quercetin-3-O-rhamnoside (2)와 quercetin-3-O-glucoside(3)로 구조 동정되었다. 분리된 화합물들의 생리활성은 matrix metalloproteinase-2 저해 활성과 인체암 세포주에 대한 세포독성과 apoptosis실험을 통하여 항암효과를 조사하였다. 그 결과 인체암 세포주에 대한 세포독성과 apoptosis 저해활성은 나타내지 않았으나, MMP-2활성저해 조사에서는 강한 저해 효과를 나타내었다. 특히 quercetin-3-O-glucoside는 암세포에 직접적인 독성을 보이는 것이 아니라 암의 침윤과 전이에 특이적으로 작용하는 물질로 보여진다.
김현태(Hyun Tae Kim),고종민(Jong Min Ko),한원영(Won Young Han),백인열(In Youl Baek),윤홍태(Hong Tae Yun),이영훈(Young Hoon Lee),이병원(Byoung Won Lee),하태정(Tae Joung Ha),정찬식(Chan Sik Jung),신상욱(Sang Ouk Shin),김홍식(Hong Sik K 한국육종학회 2018 한국육종학회지 Vol.50 No.3
‘Haepum’ is a soybean cultivar for soy sprouts. ‘Bosug’ and ‘Suwon 214’ were crossed in 2001, and promised lines were selected from F3 to F5 using the pedigree method (Pedigree: HS1273-2B-4-1-2-3-3). Preliminary yield (PYT) and advanced yield (AYT) trials were conducted from 2008 to 2009, and regional yield trials (RYT) in four regions (Suwon, Naju, Miryang, Jeju) were conducted from 2010 to 2012. In RYT, ‘Haepum’ had higher plant height and first pod height than other cultivars, which are desirable characteristics for mechanized harvest. Morphological characteristics of ‘Haepum’ are as follows: determinate, white flowers, grey pubescence, yellow pods, yellow spherical small seeds (10.4 g/100 seeds), and light brown hilum. The flowering and maturity dates were July 29 and October 6, respectively. Plant height was 61 cm, 6 cm longer than that of ‘Pungsannamulkong’ (standard cultivar). Moreover, the first pod height of ‘Haepum’ was 15 cm, 6 cm longer than that of ‘Pungsannamulkong’. The lodging tolerance was also stronger (1) than that of ‘Pungsannamulkong’ (6). The germination rate, total length, hypocotyl length, thickness, and sprout yield of ‘Haepum’ soybean sprouts were 99%, 20.6 cm, 9.7 cm, 2.1 mm, and 453%, respectively. The yield in adaptable regions was 301 kg/10 a, which was 10% higher than that of ‘Pungsannamulkong’. ‘Haepum’ could be broadly used for soybean sprout culture and an adequate cultivar for conversion to jeju and mechanized harvesting.
이경동 ( Kyung Bong Lee ),하태정 ( Tae Joung Ha ),이병원 ( Byong Won Lee ),이종록 ( Jong Rok Lee ),이준 ( Jun Lee ),황선우 ( Sun Woo Hwang ),조동령 ( Dong Young Cho ),남상해 ( Sang Hae Nam ),양민석 ( Min Suk Yang ) 한국응용생명화학회 2003 Applied Biological Chemistry (Appl Biol Chem) Vol.46 No.1
Three terpenoids were isolated from the leaves of Chrysanthemum coronarium L. by silica gel chromatography and recrystalization. The structure of compounds 1, 2, and 3, using various spectroscopic data, were identified as to be terpenoid derivatives of dihydrotulipinolide, loliolide, and 5α, 8α-peroxyergosterol, respectively. The presence of these 3 is the first time reported in this plant. The cytotoxic activity of 5α, 8α-peroxyergosterol showed strong activities against human cancer cell lines such as A549, PC-3, and HCT 116.
검정콩 껍질 유래 안토시아닌의 RBL-2H3 세포에서 탈과립화와 사이토카인 생성 저해 효과
정미자(Mi Ja Chung),하태정(Tae Joung Ha),최하나(Ha Na Choi),이지선(Ji Sun Lee),박용일(Yong Il Park) 한국식품영양과학회 2011 한국식품영양과학회지 Vol.40 No.12
안토시아닌(anthocyanins)은 플라보노이드에 속하고 항산화 활성들을 포함한 그들의 다양한 건강 유익성에 대해 알려져 있다. 그들 중 검정콩 껍질에서 분리한 주요 안토시아닌은 glycopyranose를 함유하고 있는 배당체이다. 천식은 호염기성 세포(basophils)와 비만세포(mast cells)를 포함한 다양한 면역세포와 관련된 알레르기 관련 질병이다. 호산구 (eosinophils), 호염기성 세포, 비만세포는 탈과립화에 의한 천식-특이적 보조 2(T-helper 2) 사이토카인 분비 그리고 계속해서 일어나는 증폭과 같은 염증을 일으키는 매개체의 증가를 통해 알레르기 천식(allergic asthma)에서 중요한 역할을 한다. 흰쥐 호염기성 백혈병(rat basophilic leukemia) RBL-2H3 세포는 알레르기 반응을 측정하기 위해 가장 일반적으로 사용되는 in vitro 모델이다. 본 연구에서는 검정콩 껍질 안토시아닌이 항원으로 자극한 RBL-2H3 세포에서 탈 과립 그리고 Th2 사이토카인 생산에 미치는 효과를 조사하였다. 세포 탈과립은 β-hexosaminidase의 방출을 검출함으로써 평가하였다. IgE-항원 복합체로 자극한 RBL-2H3 세포내 β-hexosaminidase 방출과 Th2 사이토카인 생산이 무 처리군의 그것과 비교하여 더 높았다. 안토시아닌은 RBL-2H3 세포의 IgE-항원 복합체 유도 탈과립을 현저하게 억제 시켰고 RBL-2H3 세포내 IgE-항원 복합체-매개체 interleukin( IL)-4, IL-13 그리고 TNF-α 생산을 저해하였다. 이러한 결과는 검정콩 껍질 유래 안토시아닌이 알레르기 반응(allergic reaction)을 현저히 저해하는 효과가 있음을 보였고, 이들 안토시아닌이 향후 알레르기 천식을 억제하거나 개선하는데 유용한 물질로 사용될 가능성이 있을 것으로 판단되었다. Anthocyanins belong to a group of flavonoid compounds and are well known for their various health beneficial effects, which include antioxidative activities. Among them, the major anthocyanins isolated from seed coat of black soybean (Glycine max L.) were previously characterized as glycosides containing glucopyranose. Asthma is an allergic disease that is strongly associated with various immune cells, including basophils and mast cells. Eosinophils, basophils, and mast cells play important roles in allergic asthma through the release of inflammatory mediators such as asthma-specific T-helper 2 (Th2) cytokines and subsequent amplification of asthma symptoms via degranulation. Rat basophilic leukemia RBL-2H3 cells are the most common in vitro models for evaluating allergic reactions. In this study, we examined the effects of anthocyanin from seed coat of black soybean on antigen-stimulated degranulation and Th2 cytokine production in RBL-2H3 cells. Cell degranulation was evaluated by measuring the release of β-hexosaminidase. β-Hexosaminidase release and Th2 cytokine production in RBL-2H3 cells was much higher upon stimulation with IgE-antigen complex than those in untreated control cells. Anthocyanins significantly suppressed IgE-antigen complex-induced degranulation of RBL-2H3 cells and inhibited IgE-antigen complex-mediated interleukin (IL)-4, IL-13, and tumor necrosis factor α (TNF-α) production in RBL-2H3 cells. These findings suggest that anthocyanins from seed coat of black soybean effectively inhibit allergic reactions and may have beneficial effects against allergic asthma.