피복재료가 유채의 생육특성 및 수량에 미치는 영향

임종석(Jong Suk Lim),서석기(Sug Kee Suh),김영두(Young Doo Kim) 원광대학교 생명자원과학연구소 1992 농대논문집 Vol.15 No.-

나물콩 蒐集種의 作物學的 特性 및 種實成分含量

Sang Kyun Cho(趙湘均),Young Jin oh(吳永鎭),Sug Kee Suh(徐錫埼),Hak Sin Kim(金學信),Soo Dong Kim(金洙東),Young Sun Chang(張榮宣),Kyeong Gu Choi(崔京求) 한국육종학회 1995 한국육종학회지 Vol.27 No.2

Improvement of sprout soybeans could be accomplished by maintenance and collection of varieties which have characteristics of small seed, disease resistance and high yielding ability. This research was conducted to investigate morphological and ecological characters for 102 sprout soybean varieties collected in Chonnam and Chonbuk area, and donated by Sprout Soybean Association and Genetic Engineering Research Institute. The sprout soybean collections contained 85% purple flower color, 76% ring-type leaf, 68% brown trichome, 57% elliptical-type seed, 69% yellow seed color and 44% brown hilum color. Days to flowering ranged from 53 to 79 days and growth duration averaged 137 days, with the range of 119~149 days. Stem length ranged from 52㎝ to 165㎝ and 100-seed weight distributed into 4.9~13.3g. The grain yield revealed large range of 0.53~4.18MT/ha. Crude protein and lipid contents was ranged 35.8~48.7% (average 40.9%), and 19.1~’21.2% (average 20.1%), respectively. Correlation coefficients between grain yield and pod numbers was significantly positive, while that between 100-seed weight and pod numbers was significantly nagative. Crude protein contents was significantly associated with growth duration but negatively correlated with 100-seed weight and yield. Crude lipid contents was negatively correlated with growth duration but positively associated with 100-seed weight.

Tissue Culture and Genetic Transformation of Soybean [Glycine max (L.) Merill] with a γ-TMT Gene to Enhance α-Tocopherol Content

Kim, Young Jin,Suh, Sug Kee,Oh, Young Jin,Kim, Kyong Ho,Park, Ho Ki,Kim, Seok Dong,Yun, Song Joong 한국콩연구회 2003 韓國콩硏究會誌 Vol.20 No.1

This study was conducted to introduce a gamma-tocopherol methyl transferase gene (γ-TMT) into soybean (Glycien max) to improve tocopherol (vitamin E) composition in soybean seeds. One-day-old cotyledon after germination induced adventitious shoots (98%. 2.6 shoots/cotyledon) directly from cotyledon. The optimal medium for the induction of multiple shoots from cotyledon in 'Pungsmnamulkong'. 'Lx 16'. and 'Ilpumgeomjeongkong'was MS medium supplemented with 2 ㎎/ℓ BAP. but for 'Alchankong. MS medium supplemented with 1 ㎎/ℓ zeatin and 1 ㎎/ℓ IAA 3% sucrose. 4% phytagel and pH 5.8. Somatic embryos were induced from immature cotyledons and cultured on a MS medium containing 40 ㎎/ℓ 2.4-D. The maximum induction of embryos was obtained from cotyledons in a sue of 3-4 ㎜ at pH 7.0. The most efficient system for multiplication and development of somatic embryos is CP6 prcedure with the media MSM6AC/FNL OS3S3GM. Soy I solution (EC 2.9 mS/㎝) was proved to be the most effective solution for the acclimation. Soybean transformation with γ-TMT gene was conducted through somatic embryogenesis. Nineteen regenerated plantlets obtained on the selection medium from 1.460 cotyledons. However, only 9 plantlets were transformed. Putative selected transformants were confirmed for the stable integration of the transgene into the soybean genomic DNA by PCR and Southern blot analyses, and for the expression of the transgene by HPLC analysis. HPLC analysis showed that the content of α-tocopherol in transgenic soybean seeds (AT-I) was approximately 4-fold higher than that of non-transgenic plants. The result indicated the expression of y-TMT transgene and conversion of a part of the γ-tocopherol pool to α-tocopherol by the expressed γ-TMT.Although transformation frequency from the initial explant to whole plant was extremely low (0.7%) until now, the system can be used to transfer foreign genes into soybean cells. Gene transformation was also accomplished by in planta procedures using Agrobacterium tumefaciens. The procedure requires no tissue culture processes as it involves only direct infection of Agrobacterium at the cotyledonary nodes and adjacent regions of germinating soybean seeds or at the leaf axil of soybean seedlings. The length of time that the seeds take to germinate prior to Agrobacterium infection greatly affected infection efficiency of meristematic cells. The optimum time for the infection of germinating seeds was 24 hr after germination.

Factors on Somatic Embryogenesis and Organogenesis of Soybean (Glycine max)

Kim, Kyong-Ho,Oh, Young-Jin,Suh, Sug-Kee,Kim, Hag-Sin,Kim, Tae-Soo,Lee, Mi-Ja,Park, Ho-Ki,Suh, Duck-Yong,Yeo, Up-Dong 한국콩연구회 2002 韓國콩硏究會誌 Vol.19 No.1

To enhance in vitro plantlet regeneration efficiency of soybean through embryogenesis, the culture conditions such as meterial part and size of immature seed, 2,4-D pH and solidifying agents for somatic embryogenesis were investigated. Somatic embryogenesis was induced from the immature embryo, immature cotyledon, and embryonic axis explants of the immature seed on MS medium supplemented with 2.0 mg/L 2,4-D. The highest rate (up tp 23%) of somatic embryogenesis was obtained from the immature cotyledon, followed by embryonic axis and immature embryo. The rate varied with the developmental stages of seed. The maximum rate (25%) of embryogenesis was obtained from 3-4 mm length of the seed after 25 days of flowering. The optimum concentration of 2,4-D for embryogenesis was 10mg/l. The optimum pH was at 5.8 and solidifying agent for medium was better with 0.4% gelrite than with agar. For rapid multiplication of shoot tip from germinating somatic embryos, they were cultured on MS medium containing 2 mg/L IBA and 1 mg/L BAP, After then somatic embryos with one and three cotyledons were transferred to the plant growth regulator free medium. The medium exhibited the higher rate (50%) of development than the multiplication medium. To select the section with shoot formation ability, the calli and shoot formation from three sections of 5-days-seedings of soybean were induced on MS medium supplemented with 1.0 mg/L BAP, 3% sucrose, and 0.3% gelrite for one month. The first leaf section exhibited the highest shoot formation rate (51%), followed the hypocotyl section and the cotyledon section. The shoot formation rates and shoot number of the four excised sections of the first leaf were aldo investigated on the same medium. A half of the first leaf explant and the third of the first leaf explant had higher shoot formation rates (76-80%) and number (3-4/ explants) than those in other two explants. Effects of cytokinins on shoot formation were determined, using the half of the first leaf explants. Zeatin (1.0mg/L) exhibited the highest in shoot formation rate (94%) and combination (1: 1, v/v) of zeatin (1.0mg/L) and IAA (1.0 mg/L) eshibited the highest in shoot formation rate (96%) and numbers (16/explant), twice more than zeatin alone (1.0 mg/L). The shoot cuttings were transferred and cultivated on the rooting media supplemented with only auxin, IBA at variois concentrations. The highest root formation (8/shoot) was achieved on the medium supplementes with 1.5 mg/L. After 4 weeks of cultivation, the plantlets with an extensive root system were transplanted in pots with a soil mixture of vermiculite and fine sand. Transferred to field, about 75% of the plantlets survived.

재배조건에 따른 나물콩 품종별 콩나물 생육특성

장영선,김수동,오영진,서석기,김학신,조상균 한국콩연구회 1995 韓國콩硏究會誌 Vol.12 No.1

재배조건에 따른 나물콩 픔종별 콩나물 생육특성을 구명코자 공시 품종으로 은하콩, 이리1호, 이리2호와 이리3호를 일반재배(액비무 처리)와 액비(SAERONA, 60배액)에 각각4시간 침종후 15±1, 20±1, 25±1, 30±1℃ 등 4 수준으로 하여 수경채소재배기로 5일간 재배하 였으며, 콩나물 생육특성인 하배축장, 하배축직경, 근장, 세근수, 생중 및 수율을 치상후 2일부터 1일 간격으로 조사한 결과를 요약하면 다음과 같다. 가. 하배축장은 재배은도가 높고 100립중이 낮을수륵 일수정과에 따른 하배축신장이 큰 경향이며, 액비처리시는 일반재배에 비하여 하배축신장이 현저히 억제되었다. 나. 배축직경은 초기부터 거의 일정한 두께를 유지하였으며, 액비처리시는 일반재배에 비해 다소 두꺼웠고 품종간에는 100립중이 높은 은하콩, 이리 2호 등에서 다소 두꺼운 경향이 었다. 다. 근장과 세근수는 품퐁간에 뚜켠한 경향이 없었고, 재배온도가 높고 일수가 경과할수록 근장이 길고 세근수가 많아졌으나, 액비처리시는 근장이 현저히 억제되고 세근은 전혀 발생되지 않았다. 라. 부패립 비율은 재배온도가 높을수록 높고, 품종간에는 차이가 없으나 액비처리시는 현저한 감소 경향이었다. 마. 콩나물생증은 재배온도가 30℃까지는 높을수록 높아지는 경향으로 액비처리시는 일반재배에 비해 다소 무거웠으며 품종간에는 립중이 낮은수록 높은 경향이었다. 바. 콩나무수율은 재배온도가 30℃까지는 높을수록 높고, 품종간에는 100립중이 가벼운 품종에서 높았으며, 액비처리시는 일반재배보다 다소 높은 경향이었다. 사. 종합적인 면에서 양질 콩나물 생산에 부합되는 나물콩은 100립중이 가벼운 계통이 좋을 것으로 생각되며, 재배온도는 높을수록 수율은 많아지나 근장, 세근수 및 부패립 발생비율을 고려할 때 20∼25±1℃ 재배가 유리한 것으로 보여졌으며, 액비처리시는 세근의 발생과 부패립 발생을 억제시킬수 있으나, 액비사용에 따른 콩나물 성분분석은 금후 연구과제로 사료된다. As a primary study of soybean breeding for high quality soybean sprouts, this experiment was conducted to investigate the growing characteristics with 4 cultivars of which seed weight are different. Soybean seeds were soaked in under ground water and Saerona liquid(60 times) which is No.4 complex fertilizer for 4 hours and they were cultured under 4 different temperature conditions (15±1, 20±1, 25±1, 30±1℃) Hypocotyl length, root length and root hair number of soybean sprout are increased by the cultural temperature rise. But soybean sprout quality was not good by increase of the root length, hair number and putrefied seeds at 30±1℃. Yield ratio was high with small seed. So to grow high quality soybean sprouts, optimum culturing temperature was 20∼25±1℃ with small seed.

저장기간에 따른 나물콩 품종의 발아력 및 콩나물 특성 : 2 . 저장기간에 따른 나물콩 품종의 콩나물 특성

김영진,김경호,장영선,박호기,오영진,서석기,김학신 한국콩연구회 1996 韓國콩硏究會誌 Vol.13 No.1

기존 나물콩 품종 및 호남농업시험장 육성계통을 사용하여 저장기간에 따른 콩나물 특성을 구명하여 콩나물 재배 및 나물콩 품종육성을 위한 기초자료로 활용코자 실험하였던바, 그 결과를 용약하면 아래와 같다. 1. 25℃에서 콩나물을 5일간 재배한 결과 배축장, 근장 및 전장은 저장기간이 짧을수록 길고 세근의 발생수가 많았으며, 품종간에는 이리3호의 배축장이 가장 길고 잔뿌리의 발생은 적었다. 2. 배축직경은 저장기간에 따른 차이는 보이자 않았으며 품종간에는 립중이 낮을수록 가는 경향이었다. 3. 생육불량립율과 부패리율은 저장기간이 길어질수록 높았는데 저장기간이 4년 된 종자는 자엽 및 배축색이 갈변되기도 했으며, 5년된 종자는 발아불량으로 콩나물재배가 되지않았다. 4. 콩나물 수율은 저장기간이 짧을수록 높았으며 품종간에는 립중이 낮은 이리3호의 수율이 가장 높았다. 이상의 결과를 종합할 볼 때 콩나물 생산이 가능한 원료콩의 저장기간은 3년 이내의 것으로 보이나 생산성 및 품질면을 고려할 때 2년 이내의 종자를 사용하는 것이 안전할 것으로 판단되며, 나물콩 품종중에서는 부패 및 생육불량립율이 낮으면서 소립일수록 콩나물재배에 유리할 것으로 사료된다. This experiment was conducted to know the offect of seed storage period on the germinability and soybean - sprout (ss) characteristics using varieties for sprout soybean and breeding lines in National Honam Agricultural Experiment Station (NHAES) at NHAES in 1995, Soybean - sprout cultured for five days in incubator at 25℃. The yield of SS showed higher mainly due to long hypocotyl and root length when the sprout soybean seeds were stored for short period, while poor growth rate and high putrefaction were observed with increasing storage period. Moreover, The stored seeds for four to five years showed brownish of hypocatyl color as well as poor germination. As a results, the stared seeds for 3 years at least can be utilized for the production of SS but the seeds stored less than 2 years and with genotypes of light seed weight are considered more desirable in terms of productivity of SS and quality.