http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
고온.내산성 Bacillus sp. SJ-15를 이용한 음식물 쓰레기의 호기적 퇴비화
김춘희,남수완,최우봉,이종환,강병원,김회수,전숭종,Kim, Choon-Hee,Nam, Soo-Wan,Choi, Woo-Bong,Lee, Jong-Hwan,Kang, Byoung-Won,Kim, Hweh-Su,Jeon, Sung-Jong 한국생명과학회 2007 생명과학회지 Vol.17 No.5
고온 내산성 미생물을 퇴비에서 분리하고, 생리 및 생화학적 특성을 조사하여 Bacillus sp. SJ-15로 명명하였다. 생장을 위한 최적 온도와 pH는 각각 $55^{\circ}C$와 5.0 이었다. 분리된 SJ-15 균주는 음식물쓰레기의 고온 고속 퇴비화 공정에 적용하였다. 퇴비화 과정에서 16시간 만에 최대 생균수인 $9.2{\times}10^9/ml$를 나타내었다. 본 퇴비화 공정은 개시 100일 후에 약 88%의 감량율을 나타내었고, 퇴비성분의 농도를 분석한 결과 본 공정을 통해 생산된 퇴비는 염분을 제외하면 유기질 비료의 기준을 모두 만족하는 것으로 나타났다. A thermoacidophilic bacterium was isolated from the compost and designated as Bacillus sp. SJ-15 by physiological and biochemical characteristics. The optimum temperature and pH for growth were at $55^{\circ}C$ and pH 5.0, respectively. The strain SJ-15 was adapted in process of accelerated high-temperature composting of garbage. The highest viable cell count of composting process reached to $9.2{\times}10^9/ml$ in 16 hours. After running times of 100 days, the composting process showed a reduction rate of approximately 88%, and the concentrations of components were sufficiently high or low to satisfied the standard of organic compost except for salinity.
Bacillus stearothermophilus KJ16이 생산하는 Cyclodextrin Glucanotransferase 의 정제와 효소특성
권현주,남수완,김광현,송승구,윤종원,김병우,Kwon, Hyun-Ju,Nam, Soo-Wan,Kim, Kwang-Hyun,Song, Seong-Koo,Yun, Jong-Won,Kim, Byung-Woo 한국생명과학회 1998 생명과학회지 Vol.8 No.3
Cyclodextrin glucanotransferase from B. stearothermophilus KJ16 that can produce both cyclodextrin glucanotransferase and cyclodextrinase was purified by ammonium sulfate precipitation, DEAE-cellulose chromatography, Sephadex G-100 chromatography, and FPLC. The molecular weight of the purifice enzyme was about 65,000 dalton by SDS-PAGE. The optimal pH and temperature were 6.0 and $60^{\circ}C$, respectively. The enzyme was stable at $50^{\circ}C$ for 1 hr and in the pH range of 5.5 and 8.5. Mercaptoethanol and dithiothreitol inhibited the enzyme activity strongly. The enzyme produced 60% cyclodextrin(CD) from 5% soluble starch with the $^{\alpha}$, $^{\beta}$, $^{\gamma}$-CD ratio of 42:46:12. Amylopectin was the most suitable substrate with 67% conversion to CD.
Saccharomyces cerevisiae와 Pichia pastoris에서 Bovine Pancreatic Deoxyribonuclease I의 과발현과 특성
조은수 ( Eun Soo Cho ),김정환 ( Jeong Hwan Kim ),윤기홍 ( Ki Hong Yoon ),김연희 ( Yeon Hee Kim ),남수완 ( Soo Wan Nam ) 한국미생물생명공학회(구 한국산업미생물학회) 2012 한국미생물·생명공학회지 Vol.40 No.4
본 연구에서는 S. cerevisiae와 P. pastoris에서 bovine pancreatic (bp-) DNase I의 과발현과 재조합 DNase I의 특성을 조사하였다. bp-DNase I 유전자는 GAL10 promoter, MFα, GAL7 terminator 사이에 삽입하여 재조합 plasmid인 pGAL-MFα-DNaseI (6.4 kb)를 구축하였다. 그리고 bp-DNase I 유전자를 AOX1 promoter, MFα, AOX1 terminator에 삽입하여 재조합 plasmid인 pPEXI (8.8 kb)를 구축하였다. 재조합 plasmid인 pGAL-MFα-DNaseI과 pPEXI를 각각 S. cerevisiae와 P. pastoris 숙주세포에 형질전환시켰다. 형질전환된 효모세포들을 galactose와 methanol 배지에서 30oC, 48시간 배양하면 bp-DNase I은 대부분이 배양 상등액으로 과발현되었다. P. pastoris 형질전환체는 배양 상등액에서 45.5 unit/mL의 DNase I 활성을 보였으며, 반면에 S.cerevisiae 형질전환체는 37.7 unit/mL의 DNase I 활성을 보였다. 또한 DNA 분해 특성을 조사한 결과, P. pastoris 재조합 DNase I으로 기질 DNA(calf thymus)를 처리하였을 때 1분 이내 DNA가 분해되는 것을 확인할 수 있었으며 이는 상업용 bp-DNase I과 S. cerevisiae 재조합 DNase I으로 처리했을 때보다 빠른 분해 패턴을 보였다. In the present study, we investigated the overexpression and characterization of bovine pancreatic (bp)- DNase I in Saccharomyces cerevisiae and Pichia pastoris. The bp-DNase I gene was fused in frame with the GAL10 promoter, MFα, and GAL7 terminator sequences, resulting in the plasmid, pGAL-MFα-DNaseI (6.4 kb). Also, the bp-DNase I gene was fused in frame with the AOX1 promoter, MFα, and AOX1 terminator sequences, resulting in the plasmid, pPEXI (8.8 kb). The recombinant plasmids, pGAL-MFα-DNaseI and pPEXI were introduced into S. cerevisiae and P. pastoris host cells, respectively. When the transformed yeast cells were cultured at 30oC for 48 h in galactose or methanol medium, bp-DNase I was overexpressed and the most of activity was found in the extracellular fraction. P. pastoris transformant activity showed 45.5 unit/mL in the culture medium at 48 h cultivation, whereas S. cerevisiae transformant revealed 37.7 unit/mL in the extracellular fraction at 48 h cultivation. The enzymatic characteristics, such as DNA cleavage and half life were investigated. Treatment of the recombinant DNase I from P. pastoris induced degradation of the calf thymus DNA within 1 minute, and this DNA degradation rate was higher than that of commercial bp-DNase I (SIGMA) and the recombinant DNase I from S. cerevisiae.
Yeon-Hee Kim(김연희),Soo-Wan Nam(남수완) 한국생명과학회 2010 생명과학회지 Vol.20 No.5
복잡한 진핵생물에서의 물리적 지도 작성이나 기능해석에 효모인공염색체(YAC)를 이용하기 위해서는 원하는 target region의 인공염색체화 및 single-copy인 YAC의 복제수를 늘이는 것이 요구된다. 본 연구에서는 YAC manipulation system에 복제수 증폭시스템(copy number amplification system)을 도입한 Simultaneous YAC Manipulation-Amplification (SYMA) system을 구축하였다. 식물염색체를 가진 YAC clone의 splitting과 증폭을 위해 conditional centromere와 thymidine kinase (TK) 유전자를 가진 pBGTK plasmid를 구축하였고, splitting fragment의 PCR을 위한 주형으로 사용하였다. 590 kb의 YAC clone은 splitting과 동시에 copy number amplification element를 가진 100 kb YAC와 490 kb YAC로 분리되었고, 100 kb YAC는 유도기질로 3 ㎎/㎖ sulfanilamide와 50 μg/㎖ methotrexate (S3/M50)의 첨가에 의해 14.4배로 그 복제수가 증가하였음을 확인할 수 있었다. Artificial chromosome manipulation and amplification of single-copy yeast artificial chromosome (YAC) are usually required in order to use YACs for applications such as physical mapping and functional analysis in eukaryotes. We designed and implemented a Simultaneous YAC Manipulation- Amplification (SYMA) system that combines the copy number amplification system of YAC with a convenient YAC manipulation system. To achieve the desired split and to amplify a YAC clone-harboring plant chromosome, a pBGTK plasmid containing a conditional centromere and thymidine kinase (TK) gene was constructed as a template to amplify the splitting fragment via PCR. By splitting, new 490-kb and 100-kb split YACs containing the elements for copy number amplification were simultaneously generated from a 590-kb YAC clone. The 100-kb split YAC was then successfully amplified 14.4-fold by adding 3 ㎎/㎖ sulfanilamide and 50 ㎍/㎖ methotrexate (S3/M50) as inducing substances.