열처리에 의한 황금(Scutellaria baicalensis) 추출물의 생리활성 변화

최명락(Myeong Rak Choi),이진승(Jin Seung Lee),임현수(Hyun Soo Lim) 한국생명과학회 2007 생명과학회지 Vol.17 No.10

황금에 대한 수 추출물의 열 변환에 의한 생리활성 변화를 보기위하여 HPLC를 통한 peak분석과 기능성 평가를 하였다. 열처리 전의 시료 (CW)와 열처리 후의 시료 (CHW)간의 peak분석에서 CW와 CHW의 비교 시 A와 B가 없어지고 열처리 후에 C라는 다른 물질의 peak가 형성됨을 알 수 있었다. 또한, 전체적인 기능성 평가에서 항산화성에서는 CW와 CHW 별 차이가 없었고, 항고혈압 활성은 CW가 높았다. 항암성에 있어서 폐암에서는 CW가 높았고, 위암, 간암, 후두암 등에는 CHW가 높게 나왔다. 그리고, 멜라닌 생성세포의 멜라닌 생성 억제는 CHW에서 약 80%로 매우 높게 나타났다. 결론적으로 열처리를 통한 시료 내 구성성분의 변환이 기능성 차이를 보여주며, 이는 새로운 열 변환 물질의 생성때문으로 판단된다. 이들 변화된 물질은 황금의 주성분인 Baicalin 외의 물질이 열 변환 된 것으로, 생성된 물질에 대한 구조분석이 필요한 것으로 사료된다. To elucidate the changes in physiological activities of Scutellariae baicalensis by heating, composition analysis with HPLC and evaluation of physiological activities of extracts were conducted. HPLC analysis of CW(water extract at room temperature) and CHW(heat treated CW) indicated that two distinctive peaks in the HPLC chromatogram were disappeared after boiling and additional peak was newly observed. This observation suggested that certain chemical changed in the extract occurred by heat treatment. In the estimation of physiological activities, CW showed higher ACE inhibiting activity than CHW. In the case of cytotoxic activity against cancer cells, CW exhibited higher activity than CHW against CCL-185. On the other hand, CHW showed higher activity than CW against HeLa, SNU-601 and HepG2. In addition, CHW showed the inhibitory effect on melanogenesis (80% inhibition) while CW did not show any activity.

돌산 갓의 부위별 생리활성 작용의 비교

최명락(Myeong-Rak Choi),유은정(Eun-Jeong Yoo),송상호(Sang-Ho Song),강동수(Dong-Soo Kang),박종철(Jong-Cheol Park),임현수(Hyun-Soo Lim) 한국식품영양과학회 2001 한국식품영양과학회지 Vol.30 No.4

성장 시기에 따른 돌산 갓의 부위별 즙액 첨가에 의한 생리 기능성을 연구하였다. 생리기능성을 평가하기 위하여 돌산갓을 분쇄하여 여과하고, 원심 분리하여 얻은 상등액을 여과하여 시료로 사용하였다. 세포독성 평가를 위한 돌산 갓 즙액의 농도는 1%, 3%, 6%로 조제하여 간암세포에 첨가하였다. 세포독성은 갓 즙액 6% 첨가 시에 성장기간이 20일인 갓의 개량종 뿌리에서 78.2%로 가장 높게 나타났다. DPPH 방법에 의한 항산화 활성 평가에서는 20일 자란 재래종 잎이 80.4%로서 가장 높게 나타났다. ACE 저해 활성으로는 60일 자란 갓에서는 개량종 잎이 94.0%로서 가장 ACE 저해율이 높은 것으로 나타났다. 따라서 돌산 갓의 부위별 첨가에 의한 생리기능성 평가에서 재래종과 개량종 사이에 큰 차이를 발견할 수는 없었으나 부위별로는 항암성은 뿌리에서, 항산화성과 ACE 저해율은 잎에서 비교적 활성이 높게 나타났다. Physiological activity in different parts of Dolsan leaf mustard (DLM) was investigated. To determine the physiological activity, DLM was crushed, filtered, centrifuged and then the supernatant was used as a sample. The sample was added into HepG2 (hepatocellular carcinoma, human, ATCC) culture broth by the concentration of 1%, 3% and 6%. By adding of improved root juice (20th day) with the concentration of 6%, the cytotoxicity against HepG2 was the highest about 78.2%. And antioxidative effects in different parts of DLM was measured by using the DPPH method. Antioxidative effects was higher in all leaves than other parts. In particular, antioxidative effects was the highest in leaves of traditional DLM at the 20th day of growth about 80.4%. In leaves of improved DLM at the 60th growth, angiotensin-I converting enzyme inhibitory effect was the highest about 94.0%. Consequently, there was not significant difference of physiological activity between improved and traditional DLM. However, the cytotoxicity against HepG2 was the highest in roots of DLM. And the antioxidative and the ACE inhibitory effect in leaves of DLM were higher than those of other parts.

Angelica keiskei 지상부의 화학성분

유영법,이종호,최명락,옥광대,박종철,Yu, Young-Beob,Lee, Jong-Ho,Choi, Myeong-Rak,Ok, Kwang-Dae,Park, Jong-Cheol 한국생약학회 1996 생약학회지 Vol.27 No.1

Nucleoside, flavonol glycoside and disaccharide have been isolated from the aerial part of Angelica keiskei Koidz and identified by means of spectral analysis as adenosine, hyperoside and sucrose, respectively.

LC-PDA/MS/MS Analysis of Glucosinolates in Dolsan Leaf Mustard Kimchi and Dolsan Leaf Mustard Pickles

오선경,김기웅,최명락,Oh, Sun Kyung,Tsukamoto, Chigen,Kim, Ki Woong,Choi, Myeong Rak The Korean Society for Biotechnology and Bioengine 2016 KSBB Journal Vol.10 No.5

Changes in the concentrations of glucosinolates and related compounds in different extracts of Dolsan leaf mustard kimchi (DLMK) and Dolsan leaf mustard pickles (DLMP) were during storage investigated. Samples were kept at 0oC for 35 days and collected at 7 day intervals. The leaves and stems of DLMK and DLMP were refluxed for 24 h with 50% acetonitrile, and the extracts were analyzed by LC-PDA/MS/MS. The main glucosinolates detected in DLMK were sinigrin, gluconapoleiferin, glucobrassicanapin, and gluconapin, whereas those in DLMP were sinigrin, gluconapoleiferin, glucobrassicanapin, glucobrassicin, and glucoerucin. Sinigrin concentrations were quantified by UV absorption at 228 nm. Sinigrin concentrations in the leaves and stems of DLMK on the day of preparation were 2.14 mg/g and 2.25 mg/g, respectively, and those on day 35 after preparation were 1.25 mg/g and 1.00 mg/g, respectively. DLMP showed a similar trend: the concentrations in the leaves and stems on the day of preparation were 2.04 mg/g and 0.29 mg/g, respectively, whereas those on day 35 after preparation were 0.59 mg/g and 0.41 mg/g, respectively. Thus, sinigrin concentrations decreased during storage.

ACE and α-glucosidase Inhibitory Activity of the Glucosinolates in Dolsan Leaf Mustard Pickle during Storage

손혜련,오선경,최명락,Son, Hae-Reon,Oh, Sun-Kyung,Tsukamoto, Chigen,Choi, Myeong-Rak The Korean Society for Biotechnology and Bioengine 2016 KSBB Journal Vol.17 No.3

This study was carried out for investigate that physiological activity, quantification and qualitative were sinigrin of Dolsan leaf mustard pickle (DLMP) during storage. sinigrin contains high amounts of nutritional and medicinal compounds, which are important for maintaining optimum health. ACE inhibitory activity was ranged between 43.2 and 79.4%. DLMP methanol extracts demonstrated highest ACE inhibitory activity at 79.4% on day 14, whereas DLMP ethanol extracts demonstrated highest Angiotensin I-converting Enzyme (ACE) inhibitory activity of 43.2% at day 0. The ${\alpha}-glucosidase$ inhibitory activity of positive control 0.02% (v/v) acarbose was 78%. The DLMP methanol extracts had the highest ${\alpha}-glucosidase$ inhibitory activity at 64.0% on day 14, whereas DLMP ethanol extracts had the lowest ${\alpha}-glucosidase$ inhibitory activity of 42.8% at day 28. Sinigrin was high in DLMP methanol extracts at $49.55{\mu}g/ml$ on day 14 of storage. Sinigrin standard was eluted at 2.73 min and MS analysis was m/z 283.03 along with fragment ions at m/z 204 and 149.06. These data show that sinigrin formed desulfo-glucosinolates $[M-SO_3-2H_2O+K+2H]^+$. Sinigrin concentration increased until day 14 and then decreased after that. DLMP methanol extracts had consistently higher sinigrin concentration than DLMP acetonitrile extracts during 28 days of storage.

Quality Characteristics of Dolsan Leaf Mustard according to Various Blanching Conditions and Liquid Chromatography with Photodiode Array and Tandem Mass Spectrometry Analysis

손혜련,오선경,최명락,Son, Hae-Reon,Oh, Sun-Kyung,Tsukamoto, Chigen,Choi, Myeong-Rak The Korean Society for Biotechnology and Bioengine 2016 KSBB Journal Vol.10 No.5

This study aims to examine the optimum blanching conditions as a pretreatment condition to improve the storage stability of Dolsan leaf mustard pickle. The effects of the blan- ching temperature and time were investigated at a temperature range of $80-100^{\circ}C$. Sampling was done for 1 month after a 5 days interval. The L value of the Dolsan leaf mustard was found to be the highest at $80^{\circ}C$. The cutting force increased as the blanching temperature increased. The tensile strength decreased at $95^{\circ}C$ and $100^{\circ}C$. In addition, the sensory evaluation scores were the best at $80^{\circ}C$. The storage stability was assessed at various blanching temperatures to increase the sinigrin content during storage. Liquid chromatography with photodiode array and tandem mass spectrometry (LC-PDA/MS/MS) analysis was conducted to identify and quantify the sinigrin content in the Dolsan leaf mustard. Sinigrin as an internal standard was co-injected into each sample solution. The sample was monitored by recording the ultraviolet absorbance at 228 nm and by electrospray ionization (ESI) positive ion mode in the m/z 50-1,500 range. Blanching the sample at $80^{\circ}C$ showed the highest sinigrin concentration during storage among various temperatures and the maximum concentration was 350 ppm at 15 days storage. Study on utilization of vegetable from food processing of leaf mustard and preservation conservation results suggest that blanching at $80^{\circ}C$ is expected to improve the palatability of the pickle.

해조류 추출물과 페놀성화합물의 in vitro 및 in vivo 간보호활성

최재수,송상호,최명락,김광용,최종원,박종철,Choi, Jae-Sue,Song, Sang-Ho,Choi, Myeong-Rak,Kim, Kwang-Young,Choi, Jong-Won,Park, Jong-Cheol 한국생약학회 1997 생약학회지 Vol.28 No.4

The methanol extracts of some marine algae were tested for investigating the effects on the formation of lipid peroxide and the activities of free radical generating enzyme in vitro in bromobenzene-treated rat. The extracts of Enteromorpha compressa, Capsosiphon fulvescens, Gelidium amansii, Hizikia fusiformis, Sargassum siliquastrum and Sargassum thunbergii which decreased the formation of lipid peroxide, inhibited the activity of xanthine and aldehyde oxidases by adding of each extracts. Phloroglucinol isolated from Ecklonia stolonifera reduced bromobenzene-induced hepatic lipid peroxidation. This compound administered daily over one week before intoxication with bromobenzene did not affect the activities of aminopyrine N-demethylase, aniline hydroxylase and glu tathione S-transferase. Epoxide hydrolase activity was decreased by bromobenzene, which was restored by pretreatment of phloroglucinol, The results suggest that the bromobenzene-induced hepatic lipid peroxidation by phloroglucinol is reduced by enhancing the activity of epoxide hydrolase.

Total Polyphenol, Flavonoid Contents and Antioxidant Activities of Dolsan Leaf Mustard Pickle during Storage

손혜련,오선경,최명락,Son, Hae Reon,Oh, Sun Kyung,Choi, Myeong Rak The Korean Society for Biotechnology and Bioengine 2016 KSBB Journal Vol.6 No.2

Dolsan leaf mustard has various biological functions, including those in the immune system and cancer prevention. It contains high amounts of nutritional and medicinal compounds, which are important for maintaining optimum health. The objective of this study was to determine the total phenolics contents and antioxidant activity in Dolsan leaf mustard pickle (DLMP) during storage. DLMP methanol extracts had the highest total polyphenol and flavonoid contents of $35.56{\pm}0.01GAEmg/g$ and $4.54{\pm}0.03QEmg/g$, respectively. The DPPH and ABTS radical scavenging activities in the DLMP methanol extracts showed the highest activities of 79.4 and 85.36%, respectively. The ferric reducing antioxidant power (FRAP) assay showed 2.24-3.82 mM $FeSO_4$ eq. (p<0.05) in DLMP extracts. Overall, storage at day 14 showed the highest antioxidant activity.

피조개, Scapharca broughtonii (Schrenck) 정자의 동결보존

라성주,한경호,최명락,고강희,Rha, Sung-Ju,Han, Kyeong-Ho,Choi, Myeong-Rak,Kho, Kang-Hee 한국패류학회 2010 The Korean Journal of Malacology Vol.26 No.4

피조개, Scapharca broughtonii (Schrenck) 정자의 동결보존 위해 동결높이에 따른 예비동결 (0, 2, 4, 6 cm), 희석제 (150 mM NaCl, 300 mM glucose, Ringer's solution)와 동해방지제(glycerol, dimethylsulfoxide (DMSO), dimethylacetamide (DMA), methaol) 에 따른 효과를 정자의 운동성과 생존율로 비교하였다. 피조개 정자 동결보존에 있어 예비동결은 액체질소표면으로부터 2 cm가 가장 효과적이었으며, 희석제로는 Ringer's solution이 동해방지제로는 DMSO 10% 농도가 가장 적합하였다. This study was conducted to investigate protocol standardization for spermatozoa cryopreservation of the Scapharca broughtonii (Schrenck). Among the freezing rates, freezing at a height of 2 cm above liquid nitrogen surface for 5 minute gave higher activity and survival rate. Among the various diluents, Ringer's solution was the best for S. broughtonii sperm cryopreservation. The suitability of cryoprotectants dimethylsulfoxide (DMSO), dimethylacetamide (DMA), glycerol and methanol were tested against three freezing rates. DMSO gave significantly higher activity and survival rates than others.

Bacillus brevis로 제조한 된장의 품질

양성호(Sung-Ho Yang),최명락(Myeong-Rak Choi),지원대(Won-Dae Ji),정영건(Yung-Gun Chung),김종규(Jong-Kyu Kim) 한국식품영양과학회 1994 한국식품영양과학회지 Vol.23 No.6

Bacillus brevis로 발효한 된장은 약염기성, 황토색이었다. Amylase 액화력은 발효 5일 부터 25일 까지 약 98 D.P. unit정도 이었으며 이후 다소 감소하였고, 당화력은 5, 15, 25 및 35일에 각각 6.1, 7.2, 6.8, 6.4 S.P. unit로 나타났으며, protease 역가는 발효기간 15일 이후 급격히 증가하여 15, 25 및 35일에 각각 250, 275, 299unit로 비교적 높은 역가를 나타내었다. Bacillus brevis로 발효한 된장의 맛 성분 중 가장 많이 함유된 유리아미노산은 glutamic acid (561.8㎎%)이었으며, 유리당은 fructose가 25일째 10.25㎎%로 최고 함유량을 보였고, 비휘발성 유기산으로는 oxalic acid가 발효 15일째 12.20㎎%로 최고 함유량을 각각 나타내었다. 한편, 성분에 따라 다소 차이는 있으나 대부분 발효 25일째 최고 함유율을 보였고 이들의 함량은 재래식 된장과 비슷하였다. Bacillus brevis로 발효한 된장의 향은 25일째 이후 좋은 된장향을 나타내었으나 맛의 관능 검사치는 10일째 이후 오히려 낮아져 Bacillus brevis에 의한 된장발효는 개선이 필요하였다. We investigated the quality of soybean paste (Doenjang) fermented by Bacillus brevis. The results obtained were as follows : soybean paste fermented by Bacillus brevis had alkaline pH and yellow ochre color. Dextrinizing activity was about 98 D.P. unit from 5th to 25th day of fermentation at 30℃ and after that day somewhat decreased. Saccharifying activity was respectively 6.1, 7.2, 6.8, 6.4 S.P. unit on 5, 15, 25 and 35th day of fermentation. Protease activity suddenly increased after 15th day of fermentation and was 250, 275, 299 unit on 15, 25, 35th day of fermentation, respectively. The most abundant free amino acid was found to be glutamic acid (561.8㎎%) in soybean paste fermented by Bacillus brevis. In case of free sugar and non-volatile organic acid, fructose and oxalic acid showed highest content of 10.25㎎% on 25th day and 12.20㎎% on 15th day. The contents of free amino acids, free sugars, organic acids in soybean paste fermented by B. brevis were most abundant after 25days of fermentation and this results were similar to that of traditional soybean paste. The odor of soybean paste fermented by Bacillus brevis was improved to be a nice soybean paste odor on 25th day of fermentation. However, sensory evaluation value of the taste of it decreased after 10th day of fermentation.