Brassica 속 작물 유묘에서 장기 저온 순화처리에 따른 생화학적 변화

남민희(Min Hee Nam),박우철(Woo Churl Park),오윤진(Yun Jin Oh) 한국응용생명화학회 1995 Applied Biological Chemistry (Appl Biol Chem) Vol.38 No.3

This study was aimed for determining the biochemical mechanism of cold tolerance in crops and for searching the biochemical genetic marker related with cold tolerance by the analysis of isozyme pattern. We investigated various biochemical changes induced by the long-term cold acclimation in cold sensitive rape (B. napus) and in cold tolerant $quot;Sandongchae$quot;(B. campestris) seedlings. The cold shock after long-term cold acclimation to B. napus and B. campesris greatly increased the activities of peroxidase 157% and 50% in root fraction and, 201% and 205% in hypocotyl, respectively. Simultaneously, the activity of superoxide dismutase was largely increased in hypocotyl fraction, too. Protein contents of hypocotyl fractions in B. napus and B. campestris were also increased by 11.4% and 57.8%, respectively. The band of pI 6.4 among peroxidase isozymes newly biosynthesized during long-term cold acclimation was emerged in the hypocotyl fraction of cold tolerant it. campestris as well as in the root of both species. From above and previous results, we presented a model of interconversions of molecular oxygen species due to the cold injury and biochemically inferred the mechanism of cold tolerance in crops.

Brassica 속 작물 유묘에서 저온처리에 따른 생화학적 변화

남민희(Min Hee Nam),박우철(Woo Churl Park),박경배(Kyeong Bae Park) 한국응용생명화학회 1995 Applied Biological Chemistry (Appl Biol Chem) Vol.38 No.3

In order to determine the mechanism of cold tolerance in crops, changes in biochemical factors related with the biological reduction of molecular oxygen upon cold shock treatment were analyzed at an early stage of Brassica germination. As the cold shocked seedlings were recovered under the normal growth condition for 24 hours, the peroxidase activities in cold sensitive rape(B. napus) and cold tolerant $quot;Sandongchae$quot;(B. campestris) were considerably increased by 33% and 87% in root fraction and, 84%. and 206% in hypocotyl, respectively. The content of superoxide(O₂) in hypocotyl fraction was dramatically accumulated until 8 hours after recovery and then gradually decreased. The extent of superoxide accumulation was severer in B. napus than 13. campestris. At 24 hours after cold shock, O₂ content was decreased to the nearly control level in B. campestris but still remained by 38% in B. napus. Even though H₂O₂ content in hypocotyl fraction was decreased only 2% in B. napes during cold shock, while in B. campestris it was severely decreased about 15%. On the other hand, the cold shock at 3 days after Uniconazole treatment was more effective in increase of peroxidase activity than each separate treatment.

Brassica 속 작물 유모에서 생장억제제 Uniconazole 처리에 따른 생화학적 변화

남민희(Min Hee Nam),박우철(Woo Churl Park) 한국응용생명화학회 1995 Applied Biological Chemistry (Appl Biol Chem) Vol.38 No.3

In order to obtain the basic data for clarifing the mechanism of cold tolerance in crops, we analyzed various biochemical changes according to the Uniconazole treatment in Brassica seedling. Peroxidase activity in the root fraction of Brassica seedling was about 3 to 4 times higher than that in hypocotyl fraction, while catalase activity in those fractions showed opposite trend to the peroxidase activity. The content of hydrogen peroxide in root fraction was higher than that of hypocotyl fraction as being a reciprocal proportion with catalase activity. Especially in all fractions, peroxidase activity of $quot;Sandongchae$quot; (B. campestris) seedling, known as cold tolerant, was two-fold higher than that of cold sensitive rape(B. napus). The elongation rate of hypocotyl after germination was faster in B. napus than in B. campestris. The application of Uniconazole at 0.3 to 1.0 ppm to B. napus suppressed 43 to 46% of hypocotyl elongation and increased 65 to 73% of peroxidase activity in hypocotyl fraction. The shortening rate of hvpocotyl length due to Uniconazole treatment was positively correlated with the increasing rate of peroxidase activity in hypocotyl fraction. Superoxide dismutase was not induced upon Uniconazole treatment and has only 3 isozymes in any fractions. Its activity was observed in the order of cotyledon$gt;root$gt;hypocotyl fraction.

Anticoagulant and Fibrinolytic Activities of Hwanggeumchal Sorghum In Vitro

Min Soo Kim(김민수),In Taek Oh(오인택),Do Youn Jun(전도연),Ji Young Lee(이지영),Ho-Yong Sohn(손호용),Do Yeon Kwak(곽도연),Myung Chul Seo(서명철),Koan Sik Woo(우관식),Jee Yeon Ko(고지연),Tae Wook Jung(정태욱),Min Hee Nam(남민희),Mi He 한국생명과학회 2013 생명과학회지 Vol.23 No.12

잡곡류의 항혈전작용을 조사하기 위해 국내산 11종의 잡곡으로부터 80% 에탄올 추출물을 얻어 혈액응고저해활성을 thrombin time (TT)법으로 측정한 결과, 황금찰수수의 에탄올 추출물이 가장 높은 혈액응고저해활성을 보였으며, 뒤이어 청차조 유래의 에탄올 추출물도 혈액응고저해활성을 보였다. 황금찰수수의 에탄올 추출물을 n-hexane, methylene chloride, ethyl acetate 및 n-butanol로 분획하였을 때, 대부분의 TT-저해활성은 hexane과 methylene chloride 분획에 분포하였으며, 이들 분획의 활성은 동일 농도의 aspirin (최종 농도 480 μg/ml)보다 더 높은 것으로 나타났다. 또한 황금찰수수의 에탄올 추출물의 혈액응고저해활성은 activated partial thromboplastin time (APTT)법 및 prothrombin time (PT)법으로도 확인되었다. 이때 APTT-저해활성은 hexane 및 methylene chloride분획에 주로 분포하였으나 PT-저해활성은 hexane 분획에 주로 분포하였다. 이들 분획들의 APTT-저해활성 및 PT-저해활성은 warfarin (최종농도 3.13 mg/ml) 보다 더 높은 것으로 확인되었다. 황금찰수수 유래 에탄올 추출물의 TT-저해활성은 열 안정성 및 pH 안정성이 매우 우수하였다. 한편, 이와 같은 황금찰수수 유래 에탄올추출물, hexane 분획 및 methylene chloride 분획의 경우는 피브린 응괴를 가용화할 수 있는 피브린용해활성을 지닌 것으로 나타났다. 이러한 결과들은 황금찰수수가 혈액응고저해활성과 혈전용해활성을 지니고 있음을 보여주며, 아울러 항혈전 식이요법의 소재가 될 수 있음을 시사한다. To examine whether miscellaneous cereal grains have an antithrombotic effect, we investigated the anticoagulant activity of 80% ethanol extracts from eleven selected miscellaneous cereal grains. The 80% ethanol extract of hwanggeumchal sorghum (Sorghum bicolor) showed the highest anticoagulant activity, followed by that of green foxtail millet grains, in terms of thrombin time (TT). When the ethanol extract of hwanggeumchal sorghum was sequentially fractionated with n-hexane, methylene chloride, ethyl acetate, and n-butanol, the majority of the TT-inhibitory activity was detected in the hexane and methylene chloride fractions. Whereas aspirin (final conc. 480 μg/ml) prolonged TT by 2-fold, the ethanol extract, hexane fraction, and methylene chloride fraction in the same dose prolonged TT by 2.2-fold, 2.9-fold, and 2.5-fold, respectively. The ethanol extract of hwanggeumchal sorghum could delay activated partial thromboplastin time (APTT) as well as prothrombin time (PT). Although the APTT-inhibitory activity of the ethanol extract was mainly partitioned into the hexane and methylene chloride fractions, the PT-inhibitory activity of the ethanol extract was solely partitioned into the hexane fraction. The APTT- and PT-inhibitory activities of these organic solvent fractions were more potent than those of the control warfarin (final conc. 3.13 mg/ml). The TT-inhibitory activity of the ethanol extract was heat-stable and acid-stable. The ethanol extract, hexane fraction, and methylene chloride fraction of hwanggeumchal sorghum appeared to possess a direct fibrinolytic activity toward fibrin clotting. These results show that hwanggeumchal sorghum can exert anticoagulant and fibrinolytic effects and, thus, have the potential to be applicable as antithrombotic dietary sources.

회원작품 - 한국방송통신대학교 대전·충남지역 대학

이병구,남민희,Lee, Byeong-gu,Nam, Min-hee 대한건축사협회 2013 建築士 Vol.2013 No.6

Some Properties of Partially Purified Alkaline Inorganic Pyrophosphatase from Mung-bean (Phaseolus radiatus) Seedling

박우철,남민희,Park, Woo-Churl,Nam, Min-Hee 한국토양비료학회 1982 한국토양비료학회지 Vol.15 No.4

발아초기 녹두의 부위별 alkaline inorganic PPase의 활성변화 및 잎 부위에서 부분 정제하여 얻은 효소를 이용하여 효소적 성질을 조사한 결과를 요약하면 다음과 같다. 1. 잎 부위가 타 부위보다 약 2~4배의 더 높은 활성을 나타냈으며 발아가 진행됨에 따라 잎과 뿌리 그리고 지엽에서는 효소 활성이 초기에 증가하다가 점차 감소하는 경향을 보인 반면, 상배축에서는 초기부터 계속 감소하였으며 하배축에서는 계속 증가하는 경향을 보였다. 2. 정제 과정동안 23.9%의 수율로 86배가 정제되었으며 전기 영동상의 Rm value는 0.35였으며 homogenenity는 아니었고, Km value는 0.89mM로 나타났다. 3. 이 효소는 $Mg^{2+}$에 대해 대단히 Specific하였으며 $Cu^{2+}$와 $Fe^{2+}$도 $Mg^{2+}$에 비해 각각 56%, 55%의 activating effect를 나타냈다. 그러나, $Cu^{2+}$, $Zn^{2+}$, $Mn^{2+}$, $Co^{2+}$와 $Ni^{2+}$은 이 효소에 대해 저해체로 작용하였다. 4. 이 효소는 pH 8-9와 $50^{\circ}C$에서 최대의 활성을 보였으며 열에 대해서도 상당히 안정하였다. Time course of alkaline inorganic PPase activity with different parts of mung-bean sprout and some properties of partially purified enzyme from mung-bean leaves were investigated. The enzyme activity in leaf, root and cotyledon showed a tendency to increase at an early stage and then decrease gradually as germination continued. However, the crude homogenate of epicotyl showed the continuous decline of the enzyme activity but that of hypocotyl showed the continuous increase. In particular, the enzyme activity of leaf fraction was about 2-4 times as high as those of other fractions. The specific activity of the leaf enzyme was increased 86-fold, with a 23.9% yield, upon purification procedures. The purified enzyme from leaves had the Rm value of 0.35 and was not homogenous when judged by disc gel electrophoresis. Using tetrasodium pyrophosphate as a substrate, the apparent Km value for the partially purified enzyme was 0.89 mM. The enzyme was highly specific for $Mg^{2+}$ $CU^{2+}$ and $Fe^{2+}$ was also showed the activating effect of 56% and 55% with $Mg^{2+}$, respectively. However, $Ca^{2+}$), $Zn^{2+}$, $Mn^{2+}$, $Co^{2+}$ and $Ni^{2+}$ acted as inhibitors for the enzyme. The pH optimum for the enzyme shifted from 9.0 to 8.0 as the concentration of $Mg^{2+}$ was increased. The enzyme from mun-bean leaf was the most active at $50^{\circ}C$ and considerably stable on heat.

집합페로몬 트랩의 색깔, 설치높이 및 장소에 따른 노린재류의 유인효과

배순도,김현주,이건휘,윤영남,남민희,Bae, Soon-Do,Kim, Hyun-Ju,Lee, Geon-Hwi,Yoon, Young-Nam,Nam, Min-Hee 한국응용곤충학회 2010 한국응용곤충학회지 Vol.49 No.4

Attractiveness of stink bugs to various colors, heights and locations of fish-net traps incorporated with aggregation pheromones was determined. Bean bug, Riptortus pedestris Fabricius, was most attracted to yellow color trap, followed by white, black, green, blue and brown. R. pedestris and one-banded stink bug, Piezodorus hybneri Gmelin, were most attracted to fish-net traps placed 80 cm above the ground regardless of sexes of two species of stink bugs. Between the sexes, however, R. pedestris females were more attracted to 40 and 80 cm above the ground than the males at those heights although the attraction was not significantly different at 120 and 160 cm heights. P. hybneri females tended to be attracted to 40 and 80 cm height traps but the attraction was the opposite at 120 and 160 cm heights. The highest attraction of R. pedestris was observed in a perilla field with no significant difference in the soybean field and border area in which no crop were cultivated between the perilla and soybean fields. R. pedestris was also more attracted to the sweet persimmon orchard than the soybean and medicinal crops fields, which was a significant difference between the two fields. 집합페로몬 트랩의 색깔, 설치높이 및 장소에 따른 노린재류 유인효과를 구명하였다. 톱다리개미허리노린재는 황색트랩에 가장 많이 유인되었으며, 다음은 백색>흑색>녹색>청색>갈색의 순 이었다. 트랩설치높이에 따른 톱다리개미허리노린재와 가로줄노린재의 유인은 지상 80 cm 높이에서 성별에 관계없이 가장 많았으며, 성별간에는 톱다리개미허리노린재는 40 및 80 cm에서 암컷이 수컷보다 많이 유인되었으나, 120 및 160 cm에서 유의한 차이가 없었다. 가로줄노린재는 40 및 80 cm에서 암컷이 수컷보다 많이 유인되는 경향이었으나, 120 및 160 cm에서는 오히려 수컷이 암컷보다 약간 많이 유인되는 경향이었다. 콩 및 들깨 재배포장에서 트랩설치 위치에 따른 톱다리개미허리노린재의 유인은 들깨포장에서 가장 많았으며, 콩포장과 콩들깨의 경계지점에서는 차이가 없었다. 또한 콩포장, 약용작물포장 및 단감원에서 톱다리개미허리노린재의 유인효과는 단감원에서 가장 높았으며, 콩 및 약용작물포장간에는 유의한 차이가 없었다.

표면매입 탄소섬유판으로 보강된 철근콘크리트 부재의 휨 거동에 관한 실험연구

임동환 ( Lim¸ Dong-hwan ),남민희 ( Nam¸ Min-hee ) 한국구조물진단유지관리공학회 2008 한국구조물진단유지관리공학회 학술발표대회 논문집 Vol.12 No.1

본 연구에서는 탄소섬유판이 표면 매입된 철근 콘크리트 보의 휨 거동 효과를 고찰하였다. 이를 위하여, 탄소섬유판이 표면 매입된 T 형 철근 콘크리트 보를 제작하여 실험을 수행하였다. 본 연구 결과, 표면매입(NSM) 탄소섬유판으로 보강된 철근콘크리트 부재의 휨 강성 및 극한강도는 섬유판으로 보강되지 않은 보에 비하여 크게 증진되며, 그 최대 증가율은 보강되지 않은 부재의 경우보다 약 247%로 나타났다. 위 철근콘크리트 부재의 파괴는 부재 길이 방향으로 섬유 소선이 풀리는 표면매입 탄소 섬유판의 파괴로 시작되며, 탄소섬유판의 파단이 이어지는 2차 파괴가 발생하였다. 표면매입 탄소섬유판은 콘크리트와의 완정한 합성거동을 유도하여 따라서 탄소섬유판을 표면에 매입하는 방식은 노후 구조물 보강방식으로 매우 효과적인 것으로 판단된다.

Some Properties of Partially Purified Alkaline Inorganic Pyrophosphatase from Mung-bean (Phaseolus radiatus) Seedling

Woo-Churl Park(朴愚喆),Min-Hee Nam(南玟熙) 한국토양비료학회 1982 한국토양비료학회지 Vol.15 No.4

영남지역 시설재배지에 분포하는 Arbuscular 균근균의 포자 밀도 및 기내조건에서의 포자발아와 균사생장 특성

박향미(Hyang-Mee Park),남민희(Min-Hee Nam),강항원(Hang-Won Kang),이재생(Jae-Saeng Lee),고지연(Jee-Yeon Ko),강위금(Ui-Gum Kang),박경배(Kyeong-Bae Park) 한국토양비료학회 1999 한국토양비료학회지 Vol.32 No.2