가토의 두정골 결손부 모델에서 수산화인회석에 4-hexylresorcinol을 혼합하여 만든 인공합성골이식재의 골형성효과에 대한 연구

김민근,박용태,김성곤,박영욱,이석근,채원식,Kim, Min Keun,Park, Yong Tae,Kim, Seong-Gon,Park, Young-Wook,Lee, Suk-Keun,Choi, Weon-Sik 대한악안면성형재건외과학회 2012 Maxillofacial Plastic Reconstructive Surgery Vol.34 No.6

Purpose: The aim of this study was to evaluate the effect of 4-hexylresorcinol and hydroxyapatite combination graft on bone regeneration in the rabbit calvarial defect model. Methods: Ten New Zealand white rabbits were used for this study. Bilateral round shaped defects (diameter: 8.0 mm) were created on the parietal bone. 4-hexylresorcinol and hydroxyapatite combination graft material was grafted into the right parietal bone defect area (experimental). The left bone defect area was not filled with anything (control). The animals were sacrificed at 4 weeks and 8 weeks after grafting. A micro-computerized tomography of each specimen was taken, and the specimens were stained for histological analysis. Results: The average value of bone mineral density (BMD) and Bone volume (BV) was higher in the experimental group than in the control group at 4 weeks and 8 weeks after surgery. However, the difference was not statistically significant (P>0.05) at 8 weeks after grafting. The BMD and BV in the experimental group at 4 weeks after surgery was significantly higher than those in the control group (P<0.05). Conclusion: 4-hexylresorcinol and hydroxyapatite combination graft material showed higher initial bone formation than the control, however, there was no difference at 8weeks after operation.

느티만가닥버섯 '햇살' 품종 봉지재배 적합배지 조성 연구

김민근,심순애,김아영,권진혁,장영호,Kim, Min-Keun,Sim, Soon-Ae,Kim, Ah-Young,Kwon, Jin-Hyeuk,Chang, Young-Ho 한국버섯학회 2021 한국버섯학회지 Vol.19 No.3

느티만가닥버섯 '햇살' 품종에 대한 봉지재배 안정생산 재배기술을 개발하기 위하여 포플러(미루나무) 톱밥을 주배지로 하고 밀기울, 미강, 건비지, 면실박, 팜커널등 5종의 영양원배지 재료를 혼합하여 배지를 조성하여 재배시험을 수행하였다. 배지혼합 전 재료들에 대한 열수추출 배지들에 대한 버섯균사 생장은 팜커널에서 가장 우수하였으며 균사밀도의 경우 포플러배지를 제외하고 모두 우수하였다. 조성배지들에 대한 버섯발생 안정성, 재배기간, 수량성을 기준으로 조사한 결과 포플러톱밥, 밀기울, 미강, 건비지, 팜커널이 55:15:20:5:65(v/v) 수준으로 조합된 T6배지에서 생육기간이 단축되고 수량이 높아 봉지재배에 적합한 배지로 선발하였다. 선발배지에 대한 균사배양 완료기간은 38.0일로 대조로 이용된 T1배지에 비하여 1.7일 더 소요되었다. 그러나 균긁기 이후 수확시까지 소요되는 기간은 18.0일로 대조배지(T1)를 이용한 재배기간보다 1.0일 단축됨에 따라 총 재배기간은 68.0일이 소요되었다. 선발배지에 대한 수량은 144.7 g으로 대조배지(T1) 수량 112.5 g보다 약 28.6%가 증가되었다. 이상의 연구결과를 통해 느티만가닥버섯 '햇살' 품종의 봉지재배 적합배지로 포플러톱밥, 밀기울, 미강, 건비지, 팜커널이 55:15:20:5:5(v/v) 비율로 혼합된 배지조성을 선발하였다. 선발배지에 대한 이화학적 특성으로 수분함량은 60.8%, pH 6.0, 전질소 함량은 0.83% 수준이었다. This study was conducted to investigate a suitable substrate formulation for bag cultivation of Hypsizygus marmoreus. To determine the optimum media composition for H. marmoreus, chemical properties of various media and culture properties were investigated. The fastest primordia formation (9.0 days), the shortest fruiting body harvest period (18.0 days), and the highest yield (144.7 g/bag) were realized with the T6 formulation (55:15:20:5:5 mixing ratio of poplar sawdust, wheat bran, rice bran, dried soybean powder, and palm kernel, respectively). The chemical properties of the T6 formulation were pH, 6.0; total carbon, 19.0%; total nitrogen, 0.83%; C/N ratio, 22.8; P₂O₅, 0.61%; K₂O, 0.46%; and MgO, 0.29%. This is the first attempt using plastic bags for cultivation and will help expand the production of H. marmoreus.

양악 악교정 수술에서 르포트 I형과 U-자형 복합 골절단술 후 상악골의 안정성에 관한 임상적 연구

김민근,박영욱,Kim, Min-Keun,Park, Young-Wook 대한악안면성형재건외과학회 2009 Maxillofacial Plastic Reconstructive Surgery Vol.31 No.6

Postoperative skeletal stability was evaluated in combination of Le Fort I and U-shaped osteotomies for superior repositioning of maxilla in bi-maxillary surgeries in 30 consecutive patients. The fifteen patients underwent Le Fort I osteotomy alone and the other fifteen patients underwent Le Fort I and U-shaped osteotomies. In all patients, the maxilla was first osteomized and fixed with absorbable plates system. A bilateral sagittal split ramus osteotomy (BSSRO) of the mandible was then carried out and fixation was performed using absorbable plates. Maxillo-mandibular fixation with rubber ring was used for two weeks post-operatively in all patients. Lateral cephalograms were obtained pre-operatively, 1 day post-operatively, 6 months after surgery. The changes in anterior nasal spine (ANS), point A, upper incisior (U1), and point of maxillary tuberosity (PMT) were examined. The maxillas in the fifteen patients of both examination group were repositioned nearly in their planned positions during surgery and no significant post-operative changes in the examined points of the maxilla were found. These results suggest that a combination of a Le Fort I and U-shaped osteotomy is a useful technique for reliable superior repositioning of the maxilla. The post-operative change in the maxilla using this combination osteotomy was comparatively stable.

Cloning and Characterization of Cellulase Gene (cel5C) from Cow Rumen Metagenomic Library

Min Keun Kim(김민근),Dhirendra Nath Barman(디렌 바르만),Tae Ho Kang(강태호),Jungho Kim(김정호),Hoon Kim(김훈),Han Dae Yun(윤한대) 한국생명과학회 2012 생명과학회지 Vol.22 No.4

한우의 반추위에서 게놈 DNA를 분리하여 메타게놈 은행을 구축한 다음 섬유소분해효소를 암호화하는 유전자를 클로닝 및 유전자를 선별하였다. 선별된 유전자의 DNA 염기서열 및 아미노산 서열을 분석하고 유전산물의 생화학적인 특성을 조사하였다. cel5C 유전자는 1,125 bp로 374개의 아미노산 잔기를 가진 단백질을 암호화하였으며 이 단백질 분자량은 42 kDa이었다. 이 효소의 최적 pH는 4 근방이었으며 최적 온도는 50oC 부근이었다. cel5C 유전자의 internal primer를 사용하여 인공적으로 배양할 수 있는 49종의 반추세균에서 분리한 게놈 DNA을 주형으로 PCR 분석한 결과 해당하는 밴드를 확인할 수 없었다. Cel5C는 현재로서는 배양할 수 없는 반추 미생물로 추정된다. A metagenomic library of cow rumen in the pCC1FOS phage vector was screened in E. coli EPI300 for cellulase activity on carboxymethyl cellulose agar plates. One clone was partially digested with Sau3AI, ligated into the BamHI site of the pBluescript II SK+ vector, and transformed into E. coli DH5 α. We obtained a 1.5 kb insert DNA, designated cel5C, which hydrolyzes carboxymethyl cellulose. The cel5C gene has an open reading frame (ORF) of 1,125 bp encoding 374 amino acids. It belongs to the glycosyl hydrolase family 5 with the conserved domain LIMEGFNEIN. The molecular mass of the Cel5C protein induced from E. coli DH5α, as analyzed by CMC SDS-PAGE, appeared to be approximately 42 kDa. The enzyme showed optimum cellulase activity at pH 4.0, and 50℃. We examined whether the cel5C gene comes from the 49 identified cow rumen bacteria using PCR. No PCR bands were identified, suggesting that the cel5C gene came from the unidentified cow rumen bacteria.

보문 : 단핵균주간 교잡에 의한 큰느타리버섯 신품종 "단비"의 특성

김민근 ( Min Keun Kim ),류재산 ( Jae San Ryu ),유영복 ( Young Bok Yoo ) 한국균학회 2011 韓國菌學會誌 Vol.39 No.1

A new cultivar "Dan Bi" of Pleurotus eryngii was developed by the method of mono-mono crossing between monokaryotic strains derived from KNR2312 and KNR2596. The parental strains, KNR2312 and KNR2596, are characterized by the property of high quality and a small number of primordia formation, respectively. The optimum temperature of mycelial growth was 25 and that of fruiting body development was 15~16℃. The period of harvesting including primordia formation was 0.7~1.3 days later than that of control strain Knneutari No. 3 in the culling cultivation. The color of pileus and stipe surface was neutral-brown and pure white, respectively. The shape of pileus was dome and has a scale like as cobweb. The yield was 93±9.7 g per 850 cc of plastic bottle. Analysis of the genetic characteristics of the new commercial variety "Dan Bi" showed a different profile as that of the control strain, Knneutari No. 3, when RAPD (Random Amplified Polymorphic DNA) primer #8005 was used. This new variety "Dan Bi" of Pleurotus eryngii is characterized by a small number of primordia formation after scratching.

느티만가닥버섯에서 Penicillium brevicompactum 에 의해 유발된 곰팡이 오염 특성

김민근 ( Min-keun Kim ),심순애 ( Soon-ae Sim ),김아영 ( Ah-young Kim ),권진혁 ( Jin-hyeuk Kwon ),장영호 ( Young-ho Chang ) 한국균학회 2020 韓國菌學會誌 Vol.48 No.4

느티만가닥버섯은 일본에서 가장 많이 생산하고 소비되는 버섯으로 영양학적 가치, 우수한 식감 및 저장성 때문에 국내에서도 점진적으로 생산량이 늘어나고 있다. 2018년과 2019년 사이 경남지역 느티만가닥버섯 재배농가에서 자실체 발생이 불균일하며 수량이 감소하는 피해가 확인되었다. 분리한 오염균은 균총의 전체 표면에 회색을 띠는 진녹색포자를 형성하였다. 분리 균주의 균사생장 적합온도는 25℃로 확인되었으며 비교적 느리게 자라는 특성을 보여주었다. 분리 균주는 ITS rDNA 염기서열 분석결과Penicillium brevicompactum과 100.0%의 상동성을 나타내었고, P. brevicompactum F51로 동정 및 명명하였다. 분리 균주를 처리한 배지는 농가에서 수집된 시료와 동일하게 버섯균사의 재생을 저해하면서 발이가 46.5-71.5% 수준까지 감소되었으며, 수량은 최대 75.6%까지 감소하였다. 이러한 결과는 P. brevicompactum이 느티만가닥버섯 재배과정에서 피해를 일으킨다는 사실을 국내에서 처음 보고하는 것이다. Beech mushroom (Hypsizygus marmoreus) is one of the most popular mushrooms in Japan, China, and Korea because of its delicious taste, nutritious value, and unique crunchy texture. In 2018-2019, unusual signs on the mushroom were observed in mushroom farms in Gyeongnam Province. The main signs were the inhibition of mycelial growth and primordial formation on the surface of mushroom media. When green mold caused contamination at early stage, the rate of pinhead formation by the mushroom greatly decreased to 46.5-71.5%. Conidia of the causal green mold were ellipsoidal, dark green, and measured 2.0-3.1μm long. The optimum temperature for mycelial growth of the green mold was 25℃. The phylogenetic tree obtained from the internal transcribed spacer (ITS) rDNA sequences showed that the isolated green mold corresponded to Penicillium brevicompactum (100.0%). This is the first report of green mold contamination caused by P. brevicompactum on H. marmoreus.

온도 환경 변화에 따른 평판형 TV 모서리 파손 방지를 위한 구조 설계 연구

김민근(Min Keun Kim),김성기(Sung Ki Kim) 대한기계학회 2007 대한기계학회 춘추학술대회 Vol.2007 No.5

It is conducted that study on corner crack protection for various thermal environment in a flat panel display. Most of the consumer electronics consist of a plastic and a metal structure. And different properties of materials could cause failure of structural reliability due to the various operating temperatures. Especially for front bezel with thin and slender structure, the effect of temperature is significant, and the design for crack protection is crucial for thermal reliability of displays. In this study, it is prescribed the behavior of the front bezel in flat panel display for various operation temperatures and proposed design parameters to ensure the structural reliability of displays.

보문 : 천연배지 열수추출물을 이용한 큰느타리버섯 균사배양 적합 배지 개발

김민근 ( Min Keun Kim ),류재산 ( Jae San Ryu ),이영한 ( Young Han Lee ),이성태 ( Seong Tae Lee ),허재영 ( Jae Young Heo ),권진혁 ( Jin Hyeuk Kwon ) 한국균학회 2012 韓國菌學會誌 Vol.40 No.1

센서 네트워크의 에너지 관리를 위한 TTL을 이용한 계층적 라우팅

김민근(Min-Keun Kim),최호용(Ho-Yong Choi),김준년(Joon-Nyun Kim) 한국통신학회 2005 한국통신학회 학술대회논문집 Vol.2005 No.6

수산화인회석과 인산삼칼슘 및 1형 콜라젠 혼합골의 전자빔 조사 효과

박정민,김성민,김민근,박영욱,명훈,이병철,이종호,이석근,Park, Jung Min,Kim, Soung Min,Kim, Min Keun,Park, Young Wook,Myoung, Hoon,Lee, Byung Cheol,Lee, Jong Ho,Lee, Suk Keun 대한악안면성형재건외과학회 2013 Maxillofacial Plastic Reconstructive Surgery Vol.35 No.1

Purpose: The aim of this study is to evaluate the effect and potential of electron beam (E-beam) irradiation treatment to the synthetic bony mixtures composed of hydroxyapatite (HA; Bongros$^{(R)}$, Bio@ Co., Korea) and tricalcium phosphate (${\beta}$-TCP, Sigma-Aldrich Co., USA), mixed at various ratios and of type I collagen (Rat tail, BD Biosciences Co., Sweden) as an organic matrix. Methods: We used 1.0~2.0 MeV linear accelerator and 2.0 MeV superconductive linear accelerator (power 100 KW, pressure 115 kPa, temperature $-30{\sim}120^{\circ}C$, sensor sensitivity 0.1~1.2 mV/kPa, generating power sensitivity 44.75 mV/kPa, supply voltage $5{\pm}0.25$ V) with different irradiation dose, such as 1, 30 and 60 kGy. Structural changes in this synthetic bone material were studied in vitro, by scanning electron microscopy (SEM), elementary analysis and field emission scanning electron microscope (FE-SEM), attenuated total reflection (ATR), and electron spectroscopy for chemical analysis (ESCA). Results: The large particular size of HA was changed after E-beam irradiation, to which small particle of TCP was engaged with organic collagen components in SEM findings. Conclusion: The important new in vitro data to be applicable as the substitutes of artificial bone materials in dental and medical fields will be able to be summarized.