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Lin-Hu Quan,Jin-Ying Piao,Jin-Woo Min,Ho-Bin Kim,Sang-Rae Kim,Dong-Uk Yang,Deok Chun Yang 고려인삼학회 2011 Journal of Ginseng Research Vol.35 No.3
Ginsenoside Rb<sub>1</sub>is the main component in ginsenosides. It is a protopanaxadiol-type ginsenoside that has a dammarane-type triterpenoid as an aglycone. In this study, ginsenoside Rb<sub>1</sub> was transformed into gypenoside XVII, ginsenoside Rd, ginsenoside F<sub>2</sub> and compound K by glycosidase from Leuconostoc mesenteroides DC102. The optimum time for the conversion was about 72 h at a constant pH of 6.0 to 8.0 and the optimum temperature was about 30℃. Under optimal conditions, ginsenoside Rb<sub>1</sub> was decomposed and converted into compound K by 72 h post-reaction (99%). The enzymatic reaction was analyzed by high-performance liquid chromatography, suggesting the transformation pathway: ginsenoside Rb<sub>1</sub>→gypenoside XVII and ginsenoside Rd→ginsenoside F<sub>2</sub>→compound K.
Lin-Hu Quan,Jin-Ying Piao,Jin-Woo Min,Ho-Bin Kim,Sang-Rae Kim,Dong-Uk Yang,Deok Chun Yang 고려인삼학회 2011 Journal of Ginseng Research Vol.35 No.3
Ginsenoside Rb_1is the main component in ginsenosides. It is a protopanaxadiol-type ginsenoside that has a dammarane-type triterpenoid as an aglycone. In this study, ginsenoside Rb_1 was transformed into gypenoside XVII, ginsenoside Rd, ginsenoside F_2 and compound K by glycosidase from Leuconostoc mesenteroides DC102. The optimum time for the conversion was about 72 h at a constant pH of 6.0 to 8.0 and the optimum temperature was about 30°C. Under optimal conditions, ginsenoside Rb_1was decomposed and converted into compound K by 72 h post-reaction (99%). The enzymatic reaction was analyzed by highperformance liquid chromatography, suggesting the transformation pathway: ginsenoside Rb_1→ gypenoside XVII and ginsenoside Rd→ginsenoside F_2→compound K.
Quan, Lin-Hu,Piao, Jin-Ying,Min, Jin-Woo,Kim, Ho-Bin,Kim, Sang-Rae,Yang, Dong-Uk,Yang, Deok-Chun The Korean Society of Ginseng 2011 Journal of Ginseng Research Vol.35 No.3
Ginsenoside $Rb_1$ is the main component in ginsenosides. It is a protopanaxadiol-type ginsenoside that has a dammarane-type triterpenoid as an aglycone. In this study, ginsenoside $Rb_1$ was transformed into gypenoside XVII, ginsenoside Rd, ginsenoside $F_2$ and compound K by glycosidase from Leuconostoc mesenteroides DC102. The optimum time for the conversion was about 72 h at a constant pH of 6.0 to 8.0 and the optimum temperature was about $30^{\circ}C$. Under optimal conditions, ginsenoside $Rb_1$ was decomposed and converted into compound K by 72 h post-reaction (99%). The enzymatic reaction was analyzed by highperformance liquid chromatography, suggesting the transformation pathway: ginsenoside $Rb_1$ ${\rightarrow}$ gypenoside XVII and ginsenoside Rd${\rightarrow}$ginsenoside $F_2{\rightarrow}$compound K.