Complete genome sequence of Comamonas sp. NLF-7-7 isolated from biofilter of wastewater treatment plant

김동현,한국일,권해준,김미경,김영국,최두호,이근철,서민국,김한솔,이정숙,김종국,Kim, Dong-Hyun,Han, Kook-Il,Kwon, Hae Jun,Kim, Mi Gyeong,Kim, Young Guk,Choi, Doo Ho,Lee, Keun Chul,Suh, Min Kuk,Kim, Han Sol,Lee, Jung-Sook,Kim, Jong-Guk The Microbiological Society of Korea 2019 미생물학회지 Vol.55 No.3

본 연구에서는 폐수처리장의 바이오필터로부터 Comamonas sp. NLF-7-7 균주를 분리하고 유전체서열을 PacBio RS II와 Illumina HiSeqXten 플랫폼을 사용하여 분석하였다. 염색체의 크기는 3,333,437 bp로 G + C 구성 비율은 68.04%, 총 유전자수는 3,197개, rRNA는 9개 및 tRNA는 49개로 구성되었다. 본 유전체는 오염물질분해와 플록형성에 관여하는 황산화 경로 유전자(SoxY, SoxZ, SoxA 및 SoxB)와 플록형성 경로 유전자(EpsG, EpsE, EpsF, EpsG, EpsL 및 glycosyltransferase)를 포함하고 있다. 이러한 Comamonas sp. NLF-7-7 균주는 폐수를 정화하는데 활용될 수 있다. Comamonas sp. NLF-7-7 was isolated from biofilter of wastewater treatment plant. The whole-genome sequence of Comamonas sp. NLF-7-7 was analyzed using the PacBio RS II and Illumina HiSeqXten platform. The genome comprises a 3,333,437 bp chromosome with a G + C content of 68.04%, 3,197 total genes, 9 rRNA genes, and 49 tRNA genes. This genome contained pollutants degradation and floc forming genes such as sulfur oxidization pathway (SoxY, SoxZ, SoxA, and SoxB) and floc forming pathway (EpsG, EpsE, EpsF, EpsG, EpsL, and glycosyltransferase), respectively. The Comamonas sp. NLF-7-7 can be used to the purification of wastewater.

간 질환력과 원발성 간암에 관한 환자-대조군 연구

김동현,박병주,유근영,안윤옥,이효석,김정룡,이상일,이무송,안형식,김헌,박태수,Kim, Dong-Hyun,Park, Byung-Joo,Yoo, Keun-Young,Ahn, Yoon-Ok,Lee, Hyo-Suk,Kim, Chung-Yong,Lee, Sang-Il,Lee, Moo-Song,Ahn, Hyung-Sik,Kim, Heon,Park, Tae-Soo 대한예방의학회 1994 Journal of Preventive Medicine and Public Health Vol.27 No.2

The relationship between past liver disease history and the risk of primary liver cancer was analyzed in a hospital-based case-control study conducted in Seoul on 165 patients with histologically or serologically confirmed hepatocellular carcinoma and individually age- and sex-matched 165 controls in hospital for ophthalmologic, otologic, or nasopharyngeal problems. Significant association were observed for liver deseases occurring 5 or more years before liver cancer diagnosis [OR,4.9;95% confidence interval (CI), $1.6{\sim}14.0$) and family history of liver disease(OR, 9.0;95% CI, $2.1{\sim}38.8$). These associations were not appreciably modified by allowance for major identified potential confounding factors. From these results, it is possible to speculate that liver cell injuries caused by various factors might be a common pathway to developing primary liver carcinoma. Considering the significant effect of family history of liver diseases on PLCA risk after adjusting past liver disease history, there might be genetic susceptibility in the carcinogenic mechanism of liver cancer. Further investigations are needed to clarify the effect of family history of liver disease on PLCA risk.

저강도 레이저가 흰쥐 창상 후 급성통증에 미치는 영향

김동현,백수정,김석범,송주민,김진상,Kim Dong-Hyun,Baek Su-Jeong,Kim Suk-Bum,Song Ju-Min,Kim Jin-Sang 대한물리치료학회 2002 대한물리치료학회지 Vol.14 No.2

This study was performed, using c-fos and Substance P, to investigate the effect of GaAlAs laser on acute pain model induced by wound in lumbar region's spinal level. The test group was divided into control and experimental group. Control group is shamed group(c-fos and substance P expression after non-irradiation by a GaAlAs laser), The experimental group was divided into two subgroups: one is experimental group I (c-fos expression after irradiation by a GaAlAs laser), and the other is experimental group II (substance P expression after irradiation by a GaAlAs laser). The results of this study were as following: 1. The numbers of c-fos immunoreactive neuron in spinal cord was increased markedly 1 day after wound, and decreased gradually from 1 day to 2 days in wound with GaAlAs laser irradiation. 2. The changes of the average percentages of substance P immunoreactive neurons in spinal cord was increased markedly 1 day after wound, and decreased gradually from 1 day to 2 days in wound with GaAlAs laser irradiation. Therefore, decreasing the changes of c-fos and substance P expression after irradiation by a GaAlAs laser indicates and GaAlAs laser have effect on pain control.

헤어핀 보강 선설치앵커의 정적 및 지진모의실험에 의한 전단 저항강도 평가

김동현,박용명,김태형,조성훈,강충현,Kim, Dong Hyun,Park, Yong Myung,Kim, Tae Hyung,Jo, Sung Hoon,Kang, Choong Hyun 한국강구조학회 2015 韓國鋼構造學會 論文集 Vol.27 No.3

본 연구에서는 비균열 및 균열콘크리트에 설치된 헤어핀 보강 선설치앵커의 정적 및 동적 저항강도 평가를 위한 연구를 수행하였다. 이를 위해 앵커 직경 30mm, 연단거리 150mm, 매입깊이 240mm에 D10 헤어핀 철근으로 보강한 시험체를 제작하였으며, 균열시험체는 전단하중에 직각방향과 평행방향 균열을 각각 고려하였다. 동적 강도 평가는 지진모의실험에 의하였으며 가력방법은 ACI 355.2의 기준을 적용하였다. 헤어핀 보강 앵커의 저항강도는 콘크리트 균열과는 상관성이 없었으며 동적 강도는 정적 강도와 동등한 수준을 보였다. 마지막으로 헤어핀 보강 앵커의 설계 강도에 대한 고찰을 제시하였다. This study evaluated the static and dynamic shear strength of cast-in-place anchors reinforced with hairpin bars in uncracked and cracked concrete. The anchors 30mm in diameter reinforced with D10 hairpin bar were designed with an edge distance of 150mm and an embedment depth of 240mm. The cracked specimens consisted of the orthogonal and parallel cracks to the direction of shear loads, respectively. The dynamic strength was evaluated using seismic qualification tests based on the ACI 355.2 standard. The shear strength of the hairpin reinforced anchor was hardly correlated to the concrete cracks and the dynamic strength was similar to its static shear strength. Finally, a consideration on the design strength of hairpin reinforced anchors was presented.

일본뇌염 예방접종 후 면역원성 및 중화항체 지속률에 관한 조사: 전향적 다기관 코호트 연구

김동현,홍영진,이훈재,최보율,김창휘,박재옥,강진한,최병준,김종현,안영민,주영란,정영의,한명국,Kim, Dong Hyun,Hong, Young-Jin,Lee, Hoon-Jai,Choi, Bo-Yul,Kim, Chang Hwi,Park, Jae Ock,Kang, Jin Han,Choi, Byung Joon,Kim, Jong Hyun,Ahn, Young Min 대한소아감염학회 2013 Pediatric Infection and Vaccine Vol.20 No.3

목적: 일본뇌염 백신의 면역원성 및 중화항체 지속률을 평가하여 일본뇌염 예방 사업을 위한 토대를 제공하고자 하였다. 방법: 국내 6개 대학병원에 입원한 2-6세의 어린이 중 기초접종력이 확인된 170명을 대상으로 기초접종 완료 경과 기간에 따른 중화항체가를 조사하였다. 결과: 총 170명 중 불활성화 백신 접종군 103명, 생백신 접종군 64명, 교차접종군 3명이었다. 항체검사까지의 기간은 불활성화 백신 17.5개월, 생백신 21.0개월이었고 모두 일본뇌염 방어가 가능한 항체가를 보여 불활성화 백신 322, 생백신 266이었다. 추가접종 후 항체역가 변화는 1-4개월에 가장 높았고 이후 서서히 떨어지는 경향을 보였다. 결론: 불활성화 백신과 생백신의 면역원성과 중화항체 지속률에는 차이가 없고 두 백신 모두 일본뇌염 예방을 위한 적절한 수준의 면역원성을 갖고 있었다. 향후 대규모 표본을 대상으로 더 많은 연구가 필요하다. Purpose: This study aimed to study the antibody response of Japanese encephalitis vaccination in children using different kinds of vaccines (inactivated vaccine, live attenuated vaccine or interchanged) and evaluate the effectiveness of the vaccines to provide the basis of efficient immunization schedule of Japanese encephalitis. Methods: Measurement of the neutralization antibody (NTAb) titers following Japanese encephalitis vaccination using different vaccines for 170 children, 2-6 year of age, who visited six university hospitals and are confirmed by immunization records. Results: Among 170 children who were given primary immunization on Japanese encephalitis, 103 children were given inactivated vaccine, 64 children were given live attenuated vaccine and 3 children were given interchangeably. NTAb titers were more than 1:10 in all children of three groups. The geographic mean antibody titer was 322 in inactivated vaccine group and 266 in live attenuated vaccine group. However, there was no significant difference between two groups. In both groups, the NTAb titer showed the peak at 1-4 months after the third immunization and declined. The NTAb titers of three children who were given two kinds of vaccines alternately were 1:135, 1:632, and 1:2511, respectively. Conclusion: According to the results of this study in children younger than 6 years old, there is no significant difference in effectiveness between inactivated and live attenuated vaccines. However, further studies for the changes of antibody titers for a longer period of time on larger population are required.

초등 과학영재 수업에서의 언어적 상호작용 사례 분석

김동현 ( Dong Hyun Kim ),김효남 ( Hyo Nam Kim ) 한국과학교육학회 2011 한국과학교육학회지 Vol.31 No.8

The purpose of this study was to analyze and extract the features of verbal interactions between teacher and science-gifted students in their classes. For the purpose of this study, authors observed 27 elementary science classes for the science gifted of three elementary teachers. To analyze the verbal interactions in the classes, the authors have adopted the theoretical background, which was based on Sinclair & Coulthard`s Initiative-Response- Feedback pattern. Verbal interactions in the classes were analyzed by Kim`s framework, which modified Jeong`s framework. IRF patterns were derived from the verbal interactions of science classes for the science gifted. As a result, authors obtained some important features in IRF pattern. The most commonly used IRF pattern was the teacher`s managerial question-student`s short answer pattern, which was due to the regional policy for the science gifted. The teacher`s delayed feedback as well as the teacher`s question was meaningful for student`s thinking ability. If elementary teachers consider the pattern, the strategy should be applied, which is depending on student levels and levels of contents. But three teachers did not show the characteristic verbal interaction regarded as a pattern strategy. In the future, inservice programs about verbal interaction are needed for the science gifted classes.

슬러지 폐기물을 활용한 반도체급 균일한 콜로이달 실리카 나노입자의 제조 및 CMP 응용

김동현,김지원,제갈석,김민정,김하영,김민상,김상춘,박선영,윤창민,Kim, Dong Hyun,Kim, Jiwon,Jekal, Suk,Kim, Min Jeong,Kim, Ha-Yeong,Kim, Min Sang,Kim, Sang-Chun,Park, Seon-Young,Yoon, Chang-Min 유기성자원학회 2022 유기물자원화 Vol.30 No.3

This study suggests the effective recycling method of sludge waste from various industrial fields to synthesize uniform colloidal silica nanoparticles. In detail, polymers are removed from the sludge waste to attain sludge-extracted silica (s-SiO₂) micron-sized particles, and ammonia assisted sonication is applied to s-SiO₂, which has effectively extracted the silanol precursor. The nano-sized silica (n-SiO₂) particles are successfully synthesized by a typical sol-gel method using silanol precursor. Also, the yield amounts of n-SiO₂ are determined by the function of s-SiO₂ etching time. Finally, n-SiO₂-based slurry is synthesized for the practical CMP application. As a result, rough-surfaced semiconductor chip is successfully polished by the n-SiO₂-based slurry to exhibit the mirror-like clean surface. In this regard, sludge wastes are successfully prepared as valuable semicondutor grade materials.

두께 불균일 압전 초음파 트랜스듀서의 형태에 따른 특성변화 해석

김동현,김정순,김무준,하강렬,Kim, Dong-Hyun,Kim, Jung-Soon,Kim, Moo-Joon,Ha, Kang-Lyeol 한국음향학회 2008 韓國音響學會誌 Vol.27 No.6

압전판의 길이나 폭에 따라 두께가 변화하는 압전 세라믹을 사용하여 광대역 특성을 구현하는 초음파 트랜스듀서에 대하여 압전진동자의 측면에서 본 형태에 따른 전기-음향적 특성해석을 이론적으로 수행하였다. 압전진동자의 길이방향에 따른 두께 변화를 지수함수의 조합으로 표현하고 이 함수를 이용하여 압전진동자의 전기단자에서 본 자유 어드미턴스 및 파워전달함수에 대한 식을 도출하였다. 대표적인 PZT압전 세라믹을 예로 들어 압전 진동자의 측면 형태변화에 따른 비 대역폭을 고찰해본 결과 넓은 대역폭를 얻기 위한 최적의 형태가 존재함을 알 수 있었으며, 압전진동자의 최대두께에 대한 최소두께 비가 자아짐에 따라 대역폭은 100%이상까지도 넓어질 수 있으나 파워전달함수는 반대로 감소하는 경향을 확인할 수 있었다. 또 압전진동자의 길이가 길어질수록 전달함수의 크기는 증가하나 광대역 특성을 갖는 압전진동자의 형태는 매우 한정적이 됨을 확인할 수 있었으며 이는 고효율의 광대역 초음파 트렌스듀서 제작에 있어서는 정밀한 가공이 요구됨을 확인할 수 있었다. The electro-mechanical characteristics were theoretically analyzed for the wideband ultrasonic transducer made of non-uniform thickness piezoelectric vibrator. This paper proposes a combination of exponential functions which describes the thickness variation along the length of the vibrator to derive the input admittance and power transfer function of the transducer. The bandwidth and the power transfer function of the transducer were investigated while the lateral shape of the vibrator changes. The results showed there is an optimum shape for the wideband characteristics of the transducer, and the bandwidth has increased up to over 100% as the ratio of minimum value of thickness to maximum value decreases. However, the power transfer function had a downward trend as the ratio of thickness decreases. Also we confirmed that even though the value of transfer function increases as the length of the piezoelectric vibrator increases, the shape providing wideband characteristics is very limited. It means that precision processing is required to manufacturing a wideband ultrasonic transducer with high efficiency.

ICT 기반의 인삼 공정 육묘 시 질소 형태와 게르마늄(Ge) 유무가 생장에 미치는 영향

김동현,김연복,구현정,백현진,이수빈,최지혜,홍의기,장광진,Dong Hyun, Kim,Yeon Bok, Kim,Hyun Jung, Koo,Hyun Jin, Baek,Su Bin, Lee,Jeei Hye, Choi,Eui Gi, Hong,Kwang Jin, Chang 국립한국농수산대학교 교육개발센터 2022 현장농업연구지 = Journal of practical agricultural resear Vol.24 No.4

Ginseng hydroponic cultivation was cultivated as a nutrient solution and the growth was investigated 60 days later. The length(cm) increased from EC1.0 and EC2.0 concentrations to 5.47cm before, followed by EC0.5, 2.5, 1.5, and 0.0. The weight(g) increased from EC1.0 concentration to 2.39g before, followed by EC1.5, 2.5, 0.5, 0.0 and 2.0. The width(mm) increased from EC1.0 concentration to 1.9mm than before, followed by EC2.5, 0.5, 1.5, 2.0, and 0.0. In the ginseng growth experiment according to nutritional components, the average length of NO₃-N mixed experiment increased to 0.33cm, the average weight of 0.04g, and the average width of 0.35mm. In the experiment in which NH₄-N was mixed without NO₃-N, the growth was reduced to 0.37cm in average length, 0.03g in average weight, and 0.22mm in average width. In an experiment in which germanium(Ge) is mixed with a nutrient component, the average length is increased to 0.33cm, the average weight is increased to 0.04g, and the average width is increased to 0.35mm, but in an experiment excluding germanium(Ge), the growth is reduced to 0.11cm, the average weight is 0.04g, and the average width is 0.03mm.

느타리 버섯류 ( Pleurotus spp. ) 의 생화학적 방법에 의한 품종구분

김동현,공원식,김경수,김영호,유창현,김영배 ( Dong Hyun Kim,Won Sik Kong,Kyung Soo Kim,Young Ho Kim,Chang Hyun You,Young Bae Kim ) 한국균학회 1998 韓國菌學會誌 Vol.26 No.2