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사염화탄소(四鹽化炭素)의 독성(毒性)에 미치는 Chlorpromazine 및 Iproniazid의 영향(影響)
양기천,Yang, Ki Chun 대한수의학회 1976 大韓獸醫學會誌 Vol.16 No.1
In the sequence of carbon tetrachloride hepatotoxicity, increased serum levels of a number of enzymes have been demonstrated in experimental animals. These observations, therefore, have served to help in detecting hepatic injury. The serological influence of chlorpromazine (CPZ) and iproniazid on the acute $CCl_4$ poisoning was executed in this investigation taking use of 6 albino rabbits (around 2 kg b.w.) in each group. By measuring of blood sugar level (Nelson-Somogyi method), S-GOT and S-GPT activities (Reitman-Frankel method), the pharmacological effects of the drugs was evaluated setting pretreated groups against the control. The results obtained were summarized as follows: 1. The intramuscular injection of $CCl_4$ led to increase the blood sugar level in first 3 hours and, after that, to decrease reasonably. But CPZ-pretreated group showed a tendency of increasing in compare with the control, and iproniazid-pretreated group inhibited evidently. 2. In S-GOT activity, the increased level was induced by $CCl_4$ in control. And CPZ-pretreated group showed a increased level until first day and decreased rapidly. But this property inhibited inhibited significantly by pretreating with iproniazid. 3. Although a single dose of $CCl_4$ increased the S-GPT activity, the more increasing trend was observed in CPZ-pretreated group. But these tendencies depressed remarkably in the iproniazid-pretreated group. It seemed to be attributed not to defend the $CCl_4$ toxicity but to be suppressed the enzyme systems in the liver by iproniazid that the blood sugar level and serum transaminase activities was decreased significantly in pretreating with iproniazid.
PGF<sub>2</sub>α처리(處理)에 의한 무발정(無發情) 제주마(濟州馬)의 치료효과(治療效果)
장덕지,양기천,김중계,서문현,Chang, Deog-gi,Yang, Ki-chun,Kim, Jung-kye,Suh, Moon-hean 대한수의학회 1990 大韓獸醫學會誌 Vol.30 No.3
The effects of $PGF2{\alpha}$ on the conception rate and the plasma levels of estradiol-$17{\beta}$ and progesterone of anestrus Cheju mares were investigated at the breeding and non-breeding seasons. The results obtained from this studies are as follows; 1. The durations of the estrus and diestrus after $PGF_2{\alpha}$ treatment persisted shorter than control cycle (p<0.05), but ovulation time was fast. 2. The levels of estradiol-$17{\beta}$ and progesteron before $PGF_2{\alpha}$ treatment showed 103.8pg/ml, 8.0ng/ml in breeding season and 72.8pg/ml, 4.7ng/ml in non-breeding season, respectively. 3. The levels of estradiol-$17{\beta}$ rose to 115.4~154.0pg/ml, and 90.8~27.0pg/ml from 2nd to 6th day after the treatment of $PGF_2{\alpha}$, in breeding and non-breeding seasons, respectively, while progesterone level dropped to 1ng/ml with the sign of estrus and at 8th day rose in breeding season (p<0.05). 4. Of thirty anestrus mares investigated for $PGF_2{\alpha}$ administration, 87.5% showed estrus on an average of 3.8 days after treatment and the conception rate was 62.5% in breeding season, but the estrus and conception rates dropped 40% and 20% in non-breeding season, respectively.
SDS 처리한 브루셀라 항원과 Yersinia enterocolitica 0:9주의 혈청학적 교차반응 연구
임윤규,양기천,이경갑,박전홍,이두식,박용호,강승원,목지원,이영순,Lim, Yoon-kyu,Yang, Ki-chun,Lee, Kyung-kap,Park, Jun-hong,Lee, Du-sik,Park, Yong-ho,Kang, Seung-won,Mok, Ji-won,Lee, Yong-soon 대한수의학회 1995 大韓獸醫學會誌 Vol.35 No.1
Brucella abortus cell wall antigen was extracted from Brucella abortus 1119-3 by ultrasonication and followed by sodium dodecyl sulfate(SDS) treatment. In order to confirm whether this preparation is serologically cross reactive with Yersinia enterocolitica 0:9, Western blot analysis with mouse anit-Brucella abortus1119-3 and with mouse anti-Yersinia enterocolitica 0:9 was performed. ELISA results from using those Brucella antigen and Yersinia antigen were assessed whether they had correlation. According to the results of western blot analysis and ELISA, there was no evidence of cross reactivity between the Brucella abortus 1119-3 antigen preparation and Yersinia enterocolitica 0:9. Therefore the SDS treated antigen prepared in this study could be suitably used as specific ELISA antigen without confusion in the interpretation of serological tests for brucellosis in cattle.
제주재래마(濟州在來馬)의 혈중(血中) progesterone농도측정(濃度測定)에 의한 임신진단(姙娠診斷)에 관한 연구(硏究)
장덕지,양기천,김중계,강병규,최한선,손창호,Chang, Deuk-Jee,Yang, Ki-Chun,Kim, Jung-Kye,Kang, Byong-Kyu,Choi, Han-Sun,Son, Chang-Ho 대한수의학회 1991 大韓獸醫學會誌 Vol.31 No.4
Plasma progesterone concentrations were measured from 97 Che-ju native mares throughout the estrous cycle, Day 20 to 23, 5 to7 months of pregnancy, and gestation period (<1 to 7 months) using the radioimmunoassay techniques for pregnancy diagnosis. Plasma progesterone concentrations during the estrous cycle were the lowest (<1.0ng/ml) at estrus, remained high from 9 to 18 days (3.7~7.6ng/ml) and drastically decreased thereafter to reach minimal concentrations at the next estrus. Plasma progesterone concentrations at Day 21 to 24 were a significantly different between in pregnant mares (>5.7ng/ml) and in non-pregnant mares (<1.0ng/ml). The accuracy of the pregnancy diagnosis based on plasma progesterone concentrations at Day 20 to 23 was 86.7% for positive and 100% for negative, whereas on 5 to 7 months was 66.7% for positive and 71.0% for negative, respectively. Plasma progesterone concentrations during the gestation period(1 to 7 months) increased after 1 month of pregnancy, with peak concentrations(>8.0ng/ml) occurring between 60 to 100 days of pregnancy, and decreased below 1.7ng/ml after 6 months of pregnancy.
임윤규,이두식,박전홍,양기천,김승호,김공식,현관종,김우택,이영순,Lim, Yoon-kyu,Lee, Doo-sick,Park, Jun-hong,Yang, Ki-chun,Kim, Seung-ho,Kim, Kong-sick,Hyun, Kwan-jong,Kim, Woo-tack,Lee, Yong-soon 대한수의학회 1993 大韓獸醫學會誌 Vol.33 No.1
Enzyme-linked Immuno sorbent Assay (ELISA) for the serological diagnosis of Brucella abortus was developed and compared with plate agglutination test. Cell wall antigen was extracted from Brucella abortus 1119-3 by sonication and with a sodium deoxychlate solution. Optimum protein concentration of coating antigen were $0.4{\mu}g/100{\mu}{\ell}$ protein on each microtiter plate well. Horse radish peroxidase (HRP) labeled protein-G was used as a tracer of reacted antibodies. ELISA confirmed the agreeable results of 40 cases out of 43 cases by plate aggulutination test. ELISA diagnosed positive cases(10 out of 12) and negative cases (1 out of 12) with dubious sera by plate agglutination test. From this results ELISA could be used for the early diagnostic tools of Brucellosis in cattle.
국내 말로부터 비폐렴바이러스의 분리 및 면역원성에 관한 연구 I. 말 비폐렴바이러스(equine rhinopneumonitis virus)에 대한 혈청학적 역학조사
조길재,김봉환,이두식,신태균,양기천,임윤규,조성수,Cho, Gil-jae,Kim, Bong-hwan,Lee, Du-sik,Shin, Tae-kyun,Yang, Ki-chun,Lim, Yoon-kyu,Cho, Sung-soo 대한수의학회 1995 大韓獸醫學會誌 Vol.35 No.4
The present study was conducted to investigate the incidence of equine herpesvirus 1(EHV-1) infection in Korean horses by cell EUSA and serum neutralization test A significant level of antibodies to EHV-1 was detected from 539(82.8%) by cell EUSA and from 524 horses(80.5%) by SNT indicating that EHV-1 infection in Korean horses are widely spreaded throughout the country.
제주도 목야지내 독초의 분포상황 및 그 성분에 관한 조사 연구
양기천,김승호,이정택 제주대학교 1974 논문집 Vol.6 No.-
We, investigators made a survey on the species, regional distribution and toxic chemical components of the poisonous plants in Cheju island from March to Nov. 1974. The results obtained were as follows : 1. If not all, 86 species(65 spp. of herbs(inbolvlng ergot), 21 spp. of shrubs) within 42 families of the poisonous plants distributed in this Cheju area were classified. 2. According to their regional distribution, 36 spp, were in wild, field, 34 spp. in jungle area, 21 spp. in inhabited region, 15 spp. in a region of seashore, 5 spp. in a mountainous area. 3. And according to their toxic chemical components or poisoning types, they were classified as 7 spp. of cyanogenetic plants, 8 spp. of photosensitization, 6 spp. of oxalate-producing plants, 3 spp. of producing a thiamine deficiency, 2 spp. of teratogenetic effect, 1 sp (ergot) of gangrene production and 59 spp. of the other activities. 4. Actually, the bracken fern(Pteridium nquilinum) is a most problematic poisonous plant in livestock, because it is so high in frequecy or in this Cheju island. But, of course, it will be a nonsense to despise lots of the other poisonous plants.
양기천 제주대학교 농과대학 제주도축산문제연구소 1986 畜産論叢 Vol.1 No.1
Because c very little information available regarding the poisonous plants distributed in Cheju-lsland, the author made a survey of such plants from March. 1974 to Dec., 1977. The results obtained were as follows: 1. If not all, 68 families, 137 genera and 190 species of poisonous plants distributed in Cheju-island were classified. And 190 species were divided into 137 spp. of herbs, 45 spp. of shrubs, 3 spp. of molds and 5 spp. of toadstools. 2. Relatively, a large number of poisonous plants were distributed in wild field. Jungle area, inhabited region, seashore and high mountainous area took next place gradually by the number of plants. 3. And according to their toxic chemical components or poisoning types, they were clas-sified as 16 spp. of cyanogenetic plants, 14 spp. of oxalate-producing plants, 14 spp. of nitrate-producing plants, 10 spp. of photosensitization and 136 spp. of the other activities.