사료내 카드뮴 첨가가 체조직과 피모의 카드뮴, 아연, 철 및 구리함량에 미치는 영향

이근우,이현범,Lee, Keun-woo,Lee, Hyun-beom 대한수의학회 1987 大韓獸醫學會誌 Vol.27 No.2

This thesis was designed to find out whether the cadmium content of hair in living animals reflects the status of cadmium accumulation in internal organs or muscles so that this status can be used as a diagnostic method. Forty-five rats were divided into 4 experimental groups and one control group. The control group received a basal diet which contained zinc $100{\mu}g/g$, iron $80{\mu}g/g$ and copper $135{\mu}g/g$. The experimental group received experimental diets sllpplemented with 1, 10, 100 or $1,000{\mu}g/g$ cadmium as cadmium sulfate. Rats were dissected on the 28th or 56th day of experimental periods and tissues, blood and hair samples were taken, dried and burned to ash and analysed. The cadmium, iron zinc and copper concentrations were measured with an atomic absorption spectrophotometer. In addition feed efficiency and hematological changes were observed. The results obtained can be summerized as follows: A marked decrease in feed intake, weight gain and feed efficiency were observed from 1 or 2 weeks of experimental periods, which was in accordance with the dosage and periods. The cadmium contents of kidney, liver, spleen and muscle were significantly increased in all experimental rats; the accumulation was marked in kidney and liver tissues. It was shown that the cadmium contents of hair reflects the cadmium accumulation in internal organs and muscle; the higher the cadmium levels of diet and of rat tissues, the higher the cadmium content of hair. In the $100{\mu}g/g$ group of rats erythrocyte counts and. hemoglobin concentrations were decreased. A significant(p<0.01, p<0.05) increase in iron contents of kidney, liver and muscle was observed in 10, 100, $1,000{\mu}g/g$ groups of rats on the 28th day of experiment. A significant (p<0.01, p<0.05) increase in zinc contents of kidney, liver and muscle was observed in all experimental rats. On the other hand, serum zinc concentration was decreased. A significant(p<0.01) increase in copper contents in the liver was observed on the 28th day in $100{\mu}g/g$ and $1,000{\mu}g/g$ groups of rats. From these results it may be concluded that the analysis of hair for cadmium is a useful diagnostic approach for the cadmium status of internal organs and muscle in living animals.

도저용 착시 도재-금속 개면에서의 원소이동에 관한 실험적 연구

이근우,이호용,Lee, Keun-Woo,Lee, Ho-Yong 대한치과보철학회 1987 대한치과보철학회지 Vol.25 No.1

This study was undertaken to observe the elemental transition of Interface depending on different pretreatment methods, the number of filings, the kinds of porcelain powders and the various alloys in ceramo-metal restoration. The materials used in this study were Pors-on $4^{(R)}$ and Ceramco porcelain powder to compare the differences depending on the pretreatement methods and the number of firings. Ceramco porcelain powder and Vita porcelain powder were used to compare the differences of elemental transition depending on the porcelain powders. The $Parasil^{(R)},\;PGV^{(R)}$ and Pors-on $4^{(R)}$ as Pd-Ag alloy were used to compare the difference of elemental transition depending on the various alloys. The pretreatment methods were : no treatment, treatment under vacuum and air, treatment with 50 % hydrofluoric acid and double heat tretament. The number of firings were 3, 5 and 7 repeated firings All specimens were observed by SEM and concentration of elements were analyzed quantitatively with EPMA The results of this study were obtained as follows : 1. In the groups of air-treatment, concentration of Sn were the highest and widest. A high concentration of In were in the groups of vacuum and air treatment. 2. There were no significant differences in concentration of Sn between the group of vacuum, 5 minutes hydrofluoric acid and double heat treatment. (p>0.05) 3. As the period of time of hydrofluoric acid treatment increased, not only were the trace elements reduced but the main components such as Pd and Ag were also reduced. 4. Concentration of Sn and In increased and diffused with repeated firings but 5 repeated and 7 repeated firings groups had no significant differences. (p>0.05) 5. Sn were more concentrated in the group of Ceramco porcelaion powder than Vita porcelain powder. 6. The higher concentration of trace elements in the alloy, the more increasing concentration of Sn and In in the interface.

섬유주세포에서 Dipyridamole이 활성산소종과 산화스트레스에 미치는 영향

이근우,김재우,Keun Woo Lee,Jae Woo Kim 대한안과학회 2013 대한안과학회지 Vol.54 No.3

Purpose: To investigate the effects of dipyridamole (DPD) on the production of reactive oxygen species (ROS) and oxidative stress in cultured human trabecular meshwork cells (HTMC). Methods: Antioxidant activity of DPD was determined by DPPH assay. Primarily cultured HTMC were exposed to 0, 20, and 50 μm DPD using serum-deprived media. The effect of DPD on the production of ROS was assessed with the DCHFDA assay. The effect of DPD on the t-butyl hydroperoxide (tBHP)-induced oxidative stress was assessed with resazurin assay. Results: DPD showed significant antioxidant activity. DPD significantly decreased the production of ROS (p < 0.05) and improved cellular activity significantly after treatment with t-BHP (p < 0.05). DPD did not affect the generation of nitric oxides. Conclusions: DPD suppressed the formation of ROS and possessed cytoprotective activity against the oxidative stress in HTMC.

사료내의 아연과 칼슘이 돼지 부전각화증의 발생에 미치는 영향

이근우,이현범,Lee Keun-Woo,Lee Hyun-Beom 대한수의사회 1984 대한수의사회지 Vol.20 No.1

Present experiments were conducted in order to clarify the effect of dietary low zinc and excess calcium on the zinc and calcium concentrations of serum and feces, and to show the therapeutic effect of external application of zinc or soybean oil on the sk

수지접합 수복물용 합금의 피착면처리에 따른 결합력에 관한 실험적 연구

이근우,Lee, Keun-Woo 대한치과보철학회 1991 대한치과보철학회지 Vol.29 No.1

This study investigated the effects of surface treatment on the tensile bond strength of resinbonded prosthesis. The Rexillium III specimens were treated with $50{\mu}m\;Al_2O_3$ blasting. Type IV gold alloy specimens were treated with $400^{\circ}C$ heating and tin plating method. All specimens were bonded with MBAS composite resin cement and followed by immersion test into the $37^{\circ}C$ water bath for 7 days. The specimens were debonded in tension with an Instron machine and observed with SEM. The modes of failure were recorded also. The following conclusions were obtained : 1. The tensile bond strength decreased in following order. $50{\mu}m\;Al_2O_3$ basted Resillium III group, Type IV gold alloy group treated with $400^{\circ}C$ heat and tin plating type IV gold alloy group, and statistical significant differences were observed(p<0.05). 2. The tensile bond strength decreased in all groups after 7 days immersion test, but statistical significant differences were observed in Rexillium III specimens only. 3. The sharp and irregular surface were observed in Rexillium III, but $400^{\circ}C$ heat treated and tin plated groups had round and broad surface in SEM. 4. The models of bond failure were cohesive-adhesive failure mainly.

Rat에 있어서 ethanol투여가 체조직의 무기질 함량에 미치는 영향

이근우,이현범,Lee, Keun-woo,Lee, Hyun-beom 대한수의학회 1989 大韓獸醫學會誌 Vol.29 No.1

This study was designed in order to clarify the effect of ethanol drinking on the mineral contents on liver, kidney, muscle and hair. Forty-five rats were divided into 2 groups and a control group. The control group received tap water and the other 2 groups were given 8% and 40% ethanol as drinking source. Liver, kindney, muscle and hair samples were taken and analyzed for zinc, calcium and copper contents by atomic absorption spectrophotometric methods. The results obtained are summrized as follows; 1. The zinc content of muscle showed significant (p<0.01) decrease in both groups. 2. The calcium content of hair showed significant (p<0.1) increase in 8% ethanol group. 3. The copper contents of kidney and muscle in 8% ethanol group and liver in 40% ethanol group showed significant (p<0.1) decrease.

큰구슬 우렁이 (Neverita didyma) 보일드 통조림의 加熱殺菌 條件에 따른 品質變化

이근우(Keun-Woo Lee),류홍수(Hong-Soo Ryu),주현규(Hyen-Kyu Joo) 한국식품영양과학회 1984 한국식품영양과학회지 Vol.13 No.3

西海産 큰구슬우렁이의 보다 效率的인 利用方案을 모색하기 위하여 試圖한 보일드통조림의 加熱殺菌條件究明 結果는 다음과 같다.<br/> 1. 큰구슬우렁이生肉은 高蛋白, 低脂肪食品이었으며, 特히 炭水化物含量은 1% 前後였다.<br/> 2. 加熱處理時 凝固性物質은 液汁 1ml當 0.37~0.40g N. 이상에서만 凝固現象이 일어났다. 따라서 105℃, 20分, 110℃, 40分, 115℃, 20分이상 처리 시에만 發見할 수 있었다.<br/> 3. 有效性 lysine은 110℃, 20分보다는 125℃, 20分에서 12% 減少하는 반면, 20分에서 109分까지는 約 17%~22.8%의 變化를 보였다.<br/> 4. 각 處理區의 生菌檢出은 110℃, 80分, 115℃, 40分 이상에서는 檢出되지 않았다.<br/> 5. 큰구슬우렁이 生肉에서는 tryptophan이 制限아미노산이었으며, 다른 貝類의 約 57%(0.83g/16g N.) 밖에 되지 않았고, 보일드肉에서는 methionine (보유율 87%)이 가장 많이 損傷되었다.<br/> 또한 PPDRI値는 生肉 63.16, 보일드肉 65.85(115℃, 40分 處理로 生肉보다 加熱處理肉이 높았다.<br/> 6. 이상의 結果로 보아 最適加熱殺菌溫度 및 時間은 生菌數, 凝固性物質, 有效性 lysine을 감안하여 115℃, 40分 處理가 바람직하다고 생각되었다. Changes in available lysine and water soluble proteins were checked to obtain the desirable condition of processing for boiled Neverita didyma under the various autoclaving temperatures and times. It was also carried out to assess the affect of heat treatments upon the solubility of coagulable substances in boiled products.<br/> In order to evaluate the nutritional quality of processed Neverita didyma under the highest available lysine retention and without bacteria, it was studied the value of pepsin-pancreatin digest residue index in comparison with unprocessed (raw) material.<br/> The crude protein content of raw material was higher than that of the other mollusks, while the content of crude fat was showing lower value. It was noticed that the higher contents of coagulable substances and water soluble proteins in processed Neverita didyma were obtained under the conditions, retort temperature at 120℃ above, and 40-60 min. sterilization.<br/> After the pannel test, the coagulable substances were not found below the level of 0.40±0.03 gram nitrogen/ml of juice. The remarkable retention of available lysine (FDNB-reactive lysine) was resulted in the conditions of autoclaving at 115℃ for 40 minutes in boiled Neverita didyma products, and that processing condition was coincide with the condition of commercial sterilization for boiled top shell. It was revealed that the boiled meat of Neverita didyma showed the higher PPDRI(65.85) than that of raw material (63.16).

중대재해처벌법 경과와 제정 법률에 대한 비판적 검토

이근우 ( Keun-woo Lee ) 한국형사정책학회 2021 刑事政策 Vol.32 No.4

우리나라에서 산업재해로 인한 사망, 상해 사고가 지나치게 높다는 비판은 오래 전부터 이어져 왔고 이는 통계적으로도 확인된다. 또한 사람이 자신의 삶을 영위하는 직장 등의 일상공간에서 조차 안전을 담보받지 못한다는 것, 그리고 누군가의 죽음은 그 한 사람의 손실만이 아니라 그와 연결된 많은 사람들과 사회 전체에 영향을 미치게 된다는 점에서 결코 가볍게 여길 수 없는 문제라고 할 수 있다. 우리도 이 문제를 해결하기 위해 오랫동안 노력해 왔고‘중대재해처벌법’이라는 새로운 형태의 법률이 제정되었다. 이는 종전의 법안보다 책임주의에 부합하는 것으로 평가할 수 있다. 종전 법안들은 자신의 일터와 생활환경에서 안전하게 살 수 있는 환경을 만들기 위한 시도는 높이 평가할 수 있지만, 다른 대부분의 전공과 달리 상당수가 형법, 형벌이 적극적으로 활용되는 것을 꺼리도록 훈련받은 형사법학자의 입장에서 그 결과물이 반드시 지금 국회에 제출된 이러한 형태의 법안이어야 하는가 하는 의문이 적지 않았기 때문이다. 근대 이전까지 형법, 형벌은 이성적 판단의 대상이었다고 보기는 힘들 것이다. 계몽시기 이후부터 이러한 專斷 형벌에 의문을 품고 형벌의 부과대상이 되는 행위와 형벌의 종류 정도에 대해 이성적으로 접근하게 되었고, 형법학은 이러한 배경 위에서 새로운 기초 원리를 형성시켜 왔다. 형벌을 부과하는 법률이 아무리 형식적 합법성을 지닌 것이더라도 무제한으로 허용될 수 없고, 형법학의 기본 원칙을 벗어나지 않는 범위에서만 허용된다고 하는 점은 지난 30여년간 헌법재판소의 위헌법률심사의 대상이 대부분 형벌조항이었다는 점에서도 확인될 수 있을 것이다. 즉 입법권을 가진 국회의 절차를 준수한 것만으로는 불충분하고 내용적 정당성, 타당성까지도 요구되는 것이다. 산업현장과 일상 생활공간에서의 안전을 누구보다도 소망하지만, 법, 형벌만으로는 세상이 바뀌지 않는다. 많은 산업안전 분야 전문가들의 제안처럼 손쉬운 형벌의 입법, 사후처벌 중심이 아니라 비용과 노력이 들어가더라도, 근로감독관과 산업안전감독관 제도를 고용노동부에서 분리하여 독자적 책임과 판단 하에서 산업현장에서의 안전관리체계를 사전 감독, 검사하는 예방체계의 구축에 보다 많은 노력이 필요하고, 이것이 엄한 형벌의 경고만으로는 막을 수 없는 산업재해를 줄일 수 있는 길이라고 믿는다. Criticism that deaths and injuries caused by industrial accidents are too high in Korea has been criticized for a long time, and this is also statistically confirmed. In addition, the fact that a person is not guaranteed safety even in everyday spaces such as the workplace where he leads his or her life, and that the death of someone affects not only the loss of one person, but also many people connected to him and the whole society. It can be said that it is a problem that cannot be taken lightly. We have been working hard to solve this problem for a long time, and a new form of legislation called the "Severe Accident Business Punishment Act" has been submitted to the National Assembly. Attempts to create an environment where one can live safely in one's workplace and living environment can be highly appreciated, but from the standpoint of criminal law scholars, there are many questions about whether the result must be this type of bill submitted to the National Assembly now. This is because, unlike most other majors, many majors in criminal law are trained to be reluctant to actively use criminal law and punishment. Until modern times, it would be difficult to say that criminal law and punishment were the objects of rational judgment. Since the enlightenment period, they have questioned these penal punishments and have a rational approach to the types of punishments and behaviors subject to punishment, and criminal law has formed a new basic principle based on this background. In particular, Korean criminal law is strongly influenced by the German criminal law reflecting the Nazzi era. The fact that the law imposing punishment cannot be allowed indefinitely, even if it has formal legality, and that it is allowed only to the extent that it does not deviate from the basic principles of criminal law. It can be confirmed from the point. In other words, it is insufficient to comply with the procedures of the National Assembly, which has legislative powers, and content justification and validity are required. Although I wish more than anyone else for safety in industrial sites and in everyday life, the world does not change only with laws and punishments. As suggested by many experts in the field of occupational safety, even if the legislation of easy punishment, not focusing on post-punishment, but cost and effort, the labor inspector and occupational safety inspector system are separated from the Ministry of Employment and Labor, and under independent responsibility and judgment, the safety management system at the industrial site We believe that more efforts are needed to establish a preventive system that monitors and inspects in advance, and this is a way to reduce industrial accidents that cannot be prevented by warning of severe punishment alone.

MEMS 기술을 이용한 프로브 카드의 탐침 제작

이근우(Keun Woo Lee),김창교(Chang-Kyo Kim) 제어로봇시스템학회 2008 제어·로봇·시스템학회 논문지 Vol.14 No.4

Tips of probe card were fabricated using MEMS technology. P-type silicon wafer with SiO2 layer was used as a substrate for fabricating the probe card. Ni-Cr and Au used as seed layer for electroplating Ni were deposited on the silicon wafer. Line patterns for probing devices were formed on silicon wafer by electroplating Ni through mold which formed by MEMS technology. Bridge structure was formed by wet-etching the silicon substrate. AZ-1512 photoresist was used for protection layer of back side and DNB-H100PL-40 photoresist was used for patterning of the front side. The mold with the thickness of 60㎛ was also formed using THB-120N photoresist and probe tip with thickness of 50㎛ was fabricated by electroplating process.

중간수사발표에 대한 피의사실공표죄 적용의 몇 가지 쟁점

이근우 ( Keun Woo Lee ) 한국비교형사법학회 2008 비교형사법연구 Vol.10 No.1