A Study of the Two-Dimensional High-Performance Liquid Chromatogrphy for Total Amino Acid Sequencing of rbST

김정호,김훈주,김준,Kim, Jung-Ho,Kim, Hoon-Joo,Kim, Joon 생화학분자생물학회 1992 한국생화학회지 Vol.25 No.3

트립신으로 절단한 재조합 소성장호르몬의 펠타이드 조각을 분리, 정제하기 위하여 강이온 교환 HPLC와 역상 HPLC의 2차원 고성능 액체 크로마토그래피법을 이용하였다. 1차원적으로 역상 HPLC만을 이용해 펩타이드를 분리하였을 때에는 두 가지 이상의 서로 다른 펩타이드가 섞여 있는 heterogeneous peak이 발견되였다. 그러나 2차원 HPLC에 의해 펩타이드를 분리 시켰을 때에는 고도로 정제된 펩타이드를 얻을 수 있였다. 트립신 절단 결과 생긴 펩타이드를 모두 분리시키기 위해 1차적으로 강 양이온 교환 HPLC를 시행하였다. 이 결과 9개의 서로 다른 peak를 분리해 냈으며, 각각의 peak들을 다시 역상 HPLC하여 2차적인 분리를 시도하였다. 여기에서 얻은 peak들을 아미노산 서열분석을 통해 명료한 서열 분석결과를 얻어 낼 수 있었다. 또한 이 과정을 통해 53번 Cys과 164번 Cys 그리고 181번 Cys 사이에 disulfide bond가 존재하고 있음도 동시에 확인할 수 있었다. In order to separate and purify tryptic digested recombinant bST (rbST), 2-dimensional HPLC which involves a combination of strong cation-exchange chromatography and reverse-phase chromatography was applied. A single dimension RP-HPLC resulted in several heterogeneous peaks. However, all fractions purified by two-dimensional chromatogrphy resulted in homogeneous peptides and unambiguous sequence data. With a view of separating perfectly, tryptic digest was first fractionated by strong cation-exchange chromatogrphy. The nine fractions were obtained from this chromatogrphy and each of these fractions was applied to RP-HPLC. Fractions yielded from RP-HPLC were submitted for direct sequence analysis and clear sequence data were obtained. In this study, the location of disulfide bonds, Cys53-Cys164 and Cys181-Cys189, also were confirmed in rbST.

강재로 구속된 프리스트레스트 콘크리트 합성거더의 정적 및 피로거동

김정호,박경훈,황윤국,이상윤,Kim, Jung Ho,Park, Kyung Hoon,Hwang, Yoon Koog,Lee, Sang Yoon 한국강구조학회 2004 韓國鋼構造學會 論文集 Vol.16 No.6

최근 국내에서 I형 거더의 외부가 강재로 이루어지고 내부에 무근콘크리트가 채워져 합성된 거더에 프리스트레스를 도입하여 경간을 장대화할 수 있는 새로운 개념의 합성거더인 '강재로 구속된 프리스트레스트 콘크리트 합성거더(Steel-Confined Prestressed Concrete Girder; SCP Girder)'가 개발되었다. 또한 개발된 거더를 실물크기로 제작하여 시공성을 확인하고 정적하중 재하에 의한 파괴실험을 통해 공용 중 안전성에 대한 실험적 검증이 수행되었다(김정호 등, 2002). 그러나 SCP 합성거더의 실용화를 위해서는 교량 파손의 주원인이 주행하중의 영향에 의한 피로파손인 점을 고려하여 반복하중에 대한 피로거동의 연구 및 피로 안전성 확보가 요구된다. 따라서 본 연구에서는 SCP 합성거더에 대한 피로거동 특성을 파악하고 피로설계를 위한 기초적인 자료를 제시하기 위해 피로실험을 실시하였다. 피로실험을 위한 시험체의 제작은 1등교에 해당하는 설계활하중(DB-24)에 대하여 10m 지간으로 설계 제작하였으며, 실험결과 도로교설계기준(한국도로교통협회, 2000)의 상세피로범주에 따른 허용피로응력범위를 초과하지 않는 것으로 나타났다. 또한 초기정적재하를 통해 설계의 타당성과 구조적 거동을 분석하였으며, 피로실험 종료 후 거더 외부를 이루고 있는 강재를 제거하여 내부콘크리트의 상태를 관찰하였다. A new type of girder, called steel-confined prestressed concrete girder (SCP girder), has been developed, which maximizes the structural advantages of concrete, steel, and PS tendon, and improves on the shortcomings of steel plate girder, PSC I-girder, and preflex girder bridge for use in the construction of middle- or long-span bridges. To verify the propriety of design, structural safety, and applicability of this girder, a static load test was carried out (Kim et al.., 2002). Since the main damage typically sustained by steel bridges results from the fatigue caused by the repetition of traffic loads, fatigue safety must therefore be guaranteed in applying the SCP girder in the construction of real bridges. In this study, a fatigue test was carried out to investigate fatigue behavior and provide basic data for fatigue design. Based on the fatigue test, the fatigue safety of the girder was estimated. For the fatigue test, 10-m specimens were designed for a standard-design truckload (DB-24). A static load test was also performed before the fatigue test to analyze the structural behavior of the specimens. After the fatigue test, outer steel plates were removed to observe the condition of the concrete in the girder.

진동/음향 해석에 의한 스피커의 음향특성 연구

김정호,김준태,김진오,민진기,Kim, Jung-Ho,Kim, Jun-Tai,Kim, Jin-Oh,Min, Jin-Ki 대한기계학회 1997 大韓機械學會論文集A Vol.21 No.10

The acoustic characteristics of a direct radiator type loudspeaker has been studied in this paper. The natural modes of the speaker cone vibration analyzed numerically by the finite element method have been verified by comparing them with experimental results. The so-ap-proved finite-element model has been used to calculate the vibration response of the cone excited by the voice coil. The vibration displacement of the speaker cone paper has been converted into the vibration velocity and used as a boundary condition for the acoustic analysis. The frequency characteristics, directivity, and sound pressure distribution of the loudspeaker have been calculated by the boundary element method. The numerical results have been verified by the experiments carried out in an anechoic chamber. The variations of the acoustic characteristics due to the changes of some design parameter values can be examined using the numerical model.

공작실에서 실내 및 작업종사자의 중금속 오염도에 관한 고찰

김정호,김가중,김성기,배석환,Kim Jeong-Ho,Kim Gha-Jung,Kim Sung-Ki,Bea Suk-Hwan 대한방사선치료학회 2005 대한방사선치료학회지 Vol.17 No.2

Purpose : Heavy metal use when producing the block from the workshop. At this time, production of heavy metal dust and fume gives risk in human. This like heavy metal to improve seriousness through measurement and analysis. And by the quest in solution is purpose of this thesis. Materials and Methods : Organization is Inductively Coupled Plasma Atomic Emission Spectrometer, and the object is Deajeon city 4 workshops in university hospital radiation oncology (Bismuth, Lead, Tin and cadmium). Method is the ppb the pumping it does at unit, comparison analysis. And the Calculation heavy metal standard level in air through heavy metal standard level in body and blood, so Heavy metal temporary standard set. Results : Subterranean existence room air quality the administration laws appointed Lead and Cadmium's exposure recommend that it is $3{\mu}g/m^3\;and\;2{\mu}g/m^3$. And Bismuth and Tin decides $7{\mu}g/m^3\;and\;6{\mu}g/m^3$ through standard level in air heavy metal and standard level in body and blood. Heavy metal measurement level of workshops in 4 university hospital Daejeon city compares with work existence and nonexistence. On work nonexistence almost measurement level is below the recommend level. But work existence case express high level. Also consequently in composition ratio of the block is continuous with the detection ratio. Conclusion : Worker's heavy metal contamination imbrued serious for solution founds basic part. In hospital may operation on local air exhauster and periodical efficiency check, protector offer, et al. And worker have a correct understanding part of heavy metal contamination, and have continuous interest, health control. Finally, learned society sphere administer to establishment standard level and periodical measurement. And it founds basic solution plan of periodical special health checkup.

난관 세포와 공동 배양에 의한 배 세포 발달의 향상

김정호,홍기연,김기석,최정훈,민부기,Kim, Jung-Ho,Hong, Gi-Youn,Kim, Kie-Sock,Choi, Jung-Hoon,Min, Bu-Kie 대한생식의학회 1994 Clinical and Experimental Reproductive Medicine Vol.21 No.1

To improve in vitro embryonic cell development, this study was desigend to culture in vitro fertilized early embryos of mouse in two different systems; conditioned medium alone and ampullary cells co-culture. Thirty two of 83 embryos(38.6%) were blocked in the 2 cell stage by co-culture, as compared to forty of 42 embryos(95.2%) in control group for 24hours culture. And all the embryonic cells cultured for conditioned medium alone were blocked for 48 hours culture. Twenty seven of 46 embryos (58.7 %) which overcome culture block in 2 cell stage by cocultured were developed morular and expanded blastocyst, and ninteen of 46 embryos(26.1 %) underwent hatching for 96 hours culture. The cellular fragmented rates for embryo were 26.2% in medium alone; 10 fragmented blastomere were graded mild status and 1 fragmented blastomere in severe status. On the other hand, the fragmented rate for 48 hours co-cultured were 15.7%03/83); 8 fragmented embryos were graded mild status, moderate status in 3 fragmented embryos and severe in 2 fragmented embryos respectively. In conclusion, the co-culture of embryos with ampullary cells is good to improve quality of embryos and overcome of culture block as well as development of cell cleavage.

3-3'-Dimethyldiphenyl 4,4'-diisocyanate (TODI) 계 Polyurethane의 내부구조가 배향거동과 기계적 특성에 미치는 영향

김정호,이정상,이한섭,Kim, Jung-Ho,Lee, Jung-Sang,Lee, Han-Sup 한국섬유공학회 2005 한국섬유공학회지 Vol.42 No.2

New types of polyurethane (PU) have been synthesized using 3,3'-dimethyldiphenyl 4,4'-diisocyanate (TODI) in order to test the effect of the chemical structure of hard segment on the domain/segment deformation behavior and mechanical stability of polyurethane. The effect of hard segment (H/S) content on the domain/segment deformation behavior was measured with SAXS and FTIR-dichroism methods. For TODI-based PU of low H/S content, the long axes of individual hard domains of lamellar type were aligned along the uniaxial stretching direction. When the hard segment content increases, the hard domains form lamellar stacks and those stacks are either rotated or tilted towards the initial stretching depending on their H/S content. For polyurethanes of highest HIS content, the selective domain break-up phenomenon was found to be a major restructuring process. Segmental orientation behavior observed with FTIR-dichroism is consistent with domain deformation behavior observed with SAXS. Compared with MDI-based polyurethane, TODI-based polyurethane exhibits better mechanical stability and this is explained in terms of the good packing behavior of hard segments in the TODI-based polyurethanes.

자장감쇠법을 이용한 $(Bi,Pb)_2Sr_2Ca_2Cu_3O_x$/Ag 초전도선재의 접합저항 측정

김정호,이승묵,주진호,Kim, Jung-Ho,Lee, Seung-Muk,Joo, Jin-Ho 한국초전도학회 2012 Progress in superconductivity Vol.14 No.1

We fabricated a closed coils by using resistive-joint method and the joint resistance of the coils were estimated by field decay technique in liquid nitrogen. We used the Runge-kutta method for the numerical analysis to calculate the decay properties. The closed coil was wound by $(Bi,Pb)_2Sr_2Ca_2Cu_3O_x$/Ag tape. Both ends the tape were overlapped and soldered to each other. The current was induced in a closed coils by external magnetic flux density. Its decay characteristic was observed by means of measuring the magnetic flux density generated by induced current at the center of the closed coil with hall sensor. The joint resistance was calculated as the ratio of the inductance of the loop to the time constants. The joint resistances were evaluated as a function of critical current of loop, contact length, sweep time, and external magnetic flux density in a contact length of 7 cm. It was observed that joint resistance was dependent on contact length of a closed coil, but independent of critical current, sweep time, and external magnetic flux density. The joint resistance was measured to be higher for a standard four-probe method, compared with that for the field decay technique. This implies that noise of measurement in a standard four-probe method is larger than that of field decay technique. It was estimated that joint resistance was $8.0{\times}10^{-9}{\Omega}$ to $11.4{\times}10^{-9}{\Omega}$ for coils of contact length for 7 cm. It was found that 40Pb/60Sn solder are unsuitable for persistent mode.

순환골재 콘크리트 활성화를 위한 순환잔골재 품질개선 및 현장적용

김정호,이세현,김상헌,전찬수,최원영,Kim, Jung-Ho,Lee, Sea-Hyun,Kim, Sang-Heon,Jeon, Chan-Soo,Choi, Won-Young 한국건설순환자원학회 2020 한국건설순환자원학회지 Vol.15 No.1

숙지황 제조과정에서 중금속과 잔류농약

김정호,양준영,문영수,Kim, Jung-Ho,Yang, Joon-Young,Moon, Yung-Soo 환경독성보건학회 2007 환경독성보건학회지 Vol.22 No.3

본 연구에서는 건지황을 증숙하여 제조하는 숙지황 제조과정 동안의 중금속과 농약의 안전성을 평가하였다. 숙지황을 제조할 때 건지황을 황주로 9번 증숙하는 과정동안 증숙한 한국 시료의 평균은 As는 $0.147{\pm}0.007\;mg/L$이었으며, Cd는 $0.025{\pm}0.001\;mg/L$이었고, Pb는 $0.193{\pm}0.021\;mg/L$이었다. 또한 중국 시료의 평균은 As, Cd와 Pb가 각각 $0.0145{\pm}0.014\;mg/L,\;0.023{\pm}0.002\;mg/L$와 $0.299{\pm}0.013\;mg/L$이었다. 건지황을 숙지황을 제조하는 동안 중금속의 농도는 큰 변화가 없었다. 일반 시중 숙지황 중에서 As는 우리나라 시료는 평균 $0.106{\pm}0.052\;mg/L$이었으므로 As의 허용기준 3.0mg/kg에 3.53%이었으며, 중국 시료는 $0.135{\pm}0.011\;mg/L$이었으므로 As의 허용기준에 4.50%이었다. 우리나라와 중국의 숙지황 중 Cd농도는 허용기준 0.3 mg/kg에 각각 6.66%와 5.33%였다. Pb의 허용기준은 5.0 mg/kg인데 우리나라와 중국의 숙지황 중 Pb농도는 허용기준의 5.16%와 7.86%였다. 이와 같이 우리나라와 중국의 숙지황 중 중금속의 농도가 허용기준의 $3{\sim}8%$ 수준으로 매우 낮았으며, 이는 중금속으로부터 안전하였다. 우리나라 안동과 중국 하남의 건지황 중 잔류농약으로 Aldrin, $BHC-{\alpha},\;BHC-{\beta},\;BHC-{\gamma},\;BHC-{\delta}$, Captan, Chlorfenapyr, Chlorpyrifos, DDD, DDE, DDT-o,p, DDT-p,p, Dieldrin, Endosulfan-sulfate, $Endosulfan-{\alpha},\;Endosulfan-{\beta}$, Fenitrothion, Penthoate, Permethrin, Quintozene, Tolyfluanid와 Vinclozolin 등이 검출되지 않았다. 숙지황 제조에 사용된 황주 시료에도 이들 농약이 모두 동일하게 불검출로 나타났다. 따라서 이를 이용하여 제조한 숙지황에도 이들 농약이 불검출 되었다. 따라서 본 연구에서 검토된 우리나라와 중국지역의 건지황으로 제조된 숙지황은 중금속과 잔류농약이 식품의약안전청 기준에 적합한 안전성이 확인되었다. To obtain the risk assessment of hazardous materials with maturing process of Rehmanniae Radix Preparata, the residual heavy metals and pesticides in samples are surveyed. Concentration of As, Cd and Pb during the maturing process of Rehmanniae Radix Preparata of Korea are $0.147{\pm}0.007\;mg/L,\;0.025{\pm}0.001\;mg/L\;and\;0.193{\pm}0.021\;mg/L$, respectively. Concentration of As, Cd and Pb during the maturing process of Rehmanniae Radix Preparat of China are $0.0145{\pm}0.014\;mg/L,\;0.023{\pm}0.002\;mg/L\;and\;0.299{\pm}0.013\;mg/L$, respectively. Concentration of As, Cd and Hg remain unchanged during the maturing process of Rehmanniae Radix Preparata. Concentration of As, Cd and Pb in Rehmanniae Radix Preparat on the Korea are 3.53%, 6.66% and 5.16% for Korea Food & Drug Administration (KFDA) advisory level, respectively. The residual pesticides, such as aldrin, $BHC-{\alpha},\;BHC-{\beta},\;BHC-{\gamma},\;BHC-{\delta}$, captan, chlorfenapyr, chlorpyrifos, DDD, DDE, DDT-o,p, DDT-p.p, dieldrin, endosulfan-sulfate, $endosulfan-{\alpha}$, $endosulfan-{\beta}$, fenitrothion, penthoate, permethrin, quintozene, tolyfluanid and vinclozolin, are not detected in Rehmanniae Radix Preparat on the Korea and China. The hazardous materials in Rehmanniae Radix Preparata on the Korea and China are verified the safety of the residual heavy metals and pesticides compare with Korea Food & Drug Administration (KFDA) advistory level.

신산학협력 패러다임 관점에서 본 창업대학원 사업의 실태조사 연구;전국 5개 창업대학원 사례 연구를 중심으로

김정호,민경세,Kim, Jung-Ho,Min, Kyung-Se 한국벤처창업학회 2007 벤처창업연구 Vol.2 No.4

The purpose of this paper is to clarify the actual conditions of entrepreneurial school which was drawn from the new industry-academy cooperation paradigm, and to suggest a role model for educational program for entrepreneurial school, in order to increase its self-generating ability. According to survey of 5 entrepreneurial schools, most of curriculums did not show a user-based. This indicates that series of processes, such as analyzing user's(learner, local community, institution) request and setting up the goal are not being conducted professionally and systematically. Through this study, I would like to deliver the importance of new industry-academy cooperation paradigm of entrepreneurial schools and incite them to apply major principles of new industry-academy cooperation paradigm to their entrepreneurial education program, in order to enable continuous survival and development. This study has conducted research on the actual conditions of entrepreneurial school to examine whether the role model of entrepreneurial education program coincides with the paradigm of new industry-academy cooperation paradigm. Thus, this study has conducted two verifications as whole. First, through theoretical examination, this study has drawn out the major principles of new industry-academy cooperation paradigm and considered effective applying method for it. Second, this study has analyzed overseas' cases for development of entrepreneurial education program and proved the propriety of major principles. Through theory and case studies, the study presented a role models for each subjects and provided necessity for applying major principles to entrepreneurial school's research on the actual operating condition.