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(α-Amylase가 고정화된 Magnetic Nanofiber를 이용한 전분 분해공정 개발
김현,이중헌,Kim, Hyun,Lee, Jung-Heon 한국생명과학회 2007 생명과학회지 Vol.17 No.9
자석으로 분리가 가능한 ${\alpha}-Amylase$가 코팅된 나노고분자를 제조하여 녹말의 분해공정에 활용하였다. 본 연구에서 개발된 고정화 효소의 안정성은 크게 증가하여 상온에서 200rpm으로 교반하면서 보관한 경우 30일 동안에 92.7%의 활성도를 유지하였다 . 고정화 효소를 자석을 이용하여 재사용한 경우 10 회 동안 사용했을 경우 95.2%의 활성도 회수율을 보 여 효소의 재사용 가능성을 확인시켜 주었다 . 고정화 효소 0.5mg을 사용하여 녹말 분해 공정 에 활용하였을 때 2 ml의 40 g/l 녹말 용액을 40분만에 완전히 분해 시켰다, 이러한 고정화 효소를 사용하여 연속 효소반응기를 개발하여 녹말 분해공정에 활용한 결과 체류시간을 1시간으로 하였을 때 녹말 30 g/l를 76% 분해시켜 산업적으로 활용 가능성을 보여 주었다. Magnetically separable enzyme-coated nanofibers were developed for the hydrolysis of starch. Stability of ${\alpha}-amylase-coated$ nanofiber was greatly improved and its residual activity was maintained over 92.7% after 32 days incubation at room temperature and under shaking conditions (200 rpm). The recovery of enzyme was high and enzyme activity after 10 recycle was 95.2% of its original activity. Developed enzyme-coated nanofibers were used for the hydrolysis of starch. When 0.5 mg of magnetically separable enzyme nanofibers was used, 40 g/l of starch (2 ml) was completely degraded within 40 min. The continuous enzyme reactor was developed and used for starch hydrolysis and 76% of starch (30 g/l) was hydrolyzed with 1 hr residence time.
연속 발효조를 이용한 soluble glucan 생산 공정 개발
문찬준,이중헌,Moon, Chan-Jun,Lee, Jung-Heon 한국생명과학회 2006 생명과학회지 Vol.16 No.6
Continuous fermentation process for the production of soluble glucan using mutant Agrobacterium sp. ATCC31750 has been developed in this research. When the concentration of soluble glucan was higher than 6 g/l, the viscosity of the fermented broth was too high and it needs complex separation process to separate from culture broth. Mathematical models which describe the cell growth and glucan production was developed and they kinetic parameters were estimated with experimental data. They are used for the optimization of continuous fermentation process and calculate optimal dilution rate for easy separation of glucan 4 g/l. With continuous fermentation, glucan production rate was increased 1.8 times more than that with batch fermentation.
김현우 ( Hyun Woo Kim ),이중헌 ( Jung Heon Lee ) 조선대학교 공학기술연구원 2020 공학기술논문지 Vol.13 No.1
Hizikia fusiforme, a brown algae, is a major polysaccharide composed of alginate and fucoidan. In this study, the extraction of the useful substance using the organic solvent is generally avoided and a simple biocompatible / eco-friendly extraction method is devised to obtain an extract containing the water-soluble polymer polysaccharide as the main component from the dried or raw Hizikia fusiforme. In this study, the water soluble polymer polysaccharides were prepared with enzyme digestion and recovered with ethanol precipitation. The changes of reducing sugar content and protein content were observed and the molecular weight distribution was analyzed after enzyme digestion. Molecular weight measurements were performed using HPAEC and HPLC to reveal the constituents of the fraction material having anti-obesity bioactivity. The molecular weight (MW) of the extract was analyzed using a RI (Refractive Index) detector using a GPC column (PSS Suprema 1,000Å). Dextran (MW: 5, 12, 50, 150, 670 kDa) was used as a standard. As a result, polysaccharides composed of fucose, galactose and xylose (Average molecular weight: 18.5 kD) were identified. The IC50 value was determined using an enzyme extract having the best inhibition rate in the anti-obesity activity inhibition experiment. viscozyme, celluclast and alcalse were 14.06, 11.27, and 10.02 mg/ml, respectively, indicating that alcalase is most suitable.