화학물질 관리에 대한 이해 54 - 공정안전관리(PSM) 제도의 현황 및 개선방안

이주엽,Lee, Ju-Yeop 대한산업보건협회 2015 산업보건 Vol.332 No.-

산후병에 대한 자하거 치료의 실태 연구

이주엽(Ju Yeop Lee),김혜연(Hye Yeon Kim) 대한모유수유한의학회 2022 nipple confusion,breastfeeding consultation,IBCLC, Vol.3 No.1

Objectives: The purpose of this study is to examine the efficacy and safety of Hominis Placenta for postpartum disease. Methods: Pubmed, Google Scholarship, UptoDate Scholastic, OASIS and KISS were used as search sources. A combination of ‘Postpartum disease’, ‘Hominis Placenta’, ‘Puerperal wind disorder', ‘Sanhupung’, ‘Postpartum depression’, ‘Postpartum hemorrhage’, ‘Postpartum fatigue’ and ‘Postpartum hypertension’ was used as a search term. English and Korean literature were analyzed without any restrictions in the year of publication. Results: Postpartum disease refers to diseases related to childbirth during the postpartum period, which is about 6-8 weeks required for the reproductive system to recover to a non-pregnant state. Various postpartum diseases may occur if proper management is not done at this moment. Hominis Placenta is a pre-treated, dried placenta of a healthy pregnant woman. In clinical practice, it can be used to manage chronic diseases by enhancing the immune system. Conclusions: Hominis Placenta can be recommended for postpartum depression, and there is a possibility that it may be useful for postpartum bleeding, hypertension and energy loss. It cannot be considered for Puerperal Wind Disorder or postpartum abdominal pain and fever, in which the treatment of causation diseases should be preceded. In conclusion, a systematic study on the efficacy and safety of Hominis Placenta is needed.

제주조릿대 잎 잔사 추출물의 항산화 및 항염 활성

이주엽(Ju-Yeop Lee),송하나(Hana Song),고희철(Hee-Chul Ko),장미경(Mi-Gyeong Jang),김세재(Se-Jae Kim) 한국생명과학회 2018 생명과학회지 Vol.28 No.6

제주조릿대는 한라산 일대에서 자생하는 제주특산식물이다. 예로부터 조릿대 잎은 다양한 약리효과를 가지고 있어 전통의약에서 사용되어 왔을 뿐만 아니라 최근에는 식품 및 화장품 소재로서 활용되고 있다. 우리 연구팀은 제주조릿대 잎의 효율적인 산업적 활용을 촉진하기 위하여 열수 추출한 후 남는 잔사로부터 식물화합물을 다량으로 함유한 잔사 추출물(PRE)을 제조하는 방법을 보고한 바 있다. 본 연구는 PRE 및 분획물이 항산화 혹은 항염소재로서 활용 가능성을 평가하기 위하여 수행하였다. PRE 에틸아세테이트 분획물(EPRE)은 DPPH, ABTS, superoxide 자유기 소거활성이 우수하였고, LPS로 자극한 RAW 264.7 세포에서 활성 산소종(ROS)과 nitric oxide(NO) 생성을 효과적으로 억제하였다. EPRE는 nuclear factor E2-related factor 2 (Nrf 2)의 핵내 이동을 증가시킴으로써 heme oxygenase-1 (HO-1)의 발현을 유도하였다. HO-1 저해제인 zinc protoporphyrin (ZPP)은 EPRE에 의한 NO 생성 저해 능을 감소시키기 때문에 HO-1이 NO 생성 억제에 관여함을 알 수 있었다. 본 연구결과는 PRE와 EPRE가 항산화 및 항염 소재로서의 활용 가능성을 제시해 준다. Sasa quelpaertensis Nakai is a native Korean plant that grows only on Mt. Halla of Jeju Island. Its leaf is used for a popular bamboo tea due to its various health-promoting properties, and it has been increasingly used as food and cosmetic ingredients. To utilize the S. quelpaertensis leaf efficiently, the preparation method for phytochemical-rich extract (PRE) using the leaf’s residue was previously reported, which was produced after hot water extraction. This study was undertaken to evaluate the anti-oxidant and anti-inflammatory potential of PRE and its solvent fractions. The ethyl acetate fraction of PRE (EPRE) showed higher DPPH, ABTS, and superoxide radical scavenging activities, and it effectively inhibited intracellular reactive oxygen species (ROS) and nitric oxide (NO) production in lipopolysaccharides (LPS)-stimulated RAW 264.7 cells. EPRE also induced the expression of heme oxygenase-1 (HO-1) by increasing the level of nuclear factor E2-related factor 2 (Nrf2) in a nuclear fraction. The inhibiting effect of EPRE on LPS-induced NO production was partially reversed by the HO-1 inhibitor (zinc protoporphyrin, ZPP), suggesting that HO-1 is involved in suppressing NO production. Taken together, the results suggest that EPRE has potential as a promising anti- oxidant and anti-inflammatory agent.

식물화합물 다량 함유 제주조릿대 잎 추출물의 제조와 특성

이주엽(Ju Yeop Lee),고희철(Hee Chul Ko),장미경(Mi Gyeong Jang),김세재(Se Jae Kim) 한국생명과학회 2016 생명과학회지 Vol.26 No.11

조릿대 속 식물의 잎은 한의학적으로 염증, 해열, 이뇨 관련 질환 치료에 사용되어 왔다. 제주조릿대는 제주도 한라산 일대에서만 분포되어있는 토착식물이다. 본 연구에서는 제주조릿대 잎 추출물의 산업적 활용 효율을 높이기 위해 추출방법에 따른 식물화합물의 함량과 그 생리 활성을 비교함으로써 기능성이 강화된 식물화합물 다량 함유 추출물(phytochemical-rich extract; PRE)의 제조방법을 개발하였다. PRE는 열수추출물과 알코올추출물에 비하여 높은 폴리페놀 함량을 나타내었고, 조릿대 주요성분인 p-coumaric acid (44.10 mg/g)와 tricin (5.35 mg/g)의 함량은 열추추출물(p-coumaric 23.39 mg/g, tricin 0.18 mg/g)과 알코올 추출물(p-coumaric 10.8 mg/g, tricin 0.38 mg/g)에 비해 매우 높았다. RPE는 다른 추출물에 비해 총 폴리페놀 함량과 항산화 활성[1,1-diphenyl-2-picrylhydrazy (DPPH) 소거활성, 2,2"-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) 소거활성, nitric oxide (NO) 소거활성, xanthine oxidase 저해활성]이 가장 높았다. 그리고 RPE는 LPS로 자극시킨 RAW 264.7 세포에서 NO 생성(IC50 = 59.1μg/ml)과 혈액 암(HL-60) 세포의 성장을 효과적으로 억제하였다. 본 연구결과는 PRE가 식물화합물을 다량 함유하고 있어 항산화 및 항염소재로서 활용가치가 있음을 제시해 준다. Sasa species leaves have been used in traditional medicine for their anti-inflammatory, antipyretic, and diuretic properties. Sasa quelpaertensis Nakai is a small bamboo grass that grows only on Mt. Halla on Jeju Island, Republic of Korea. This small bamboo grass has recently been the focus of much attention due to its potential biomass as well as its beneficial health effects. In this study, to promote the efficient utilization of the S. quelpaertensis leaf, we established a simple preparation method for phytochemical-rich extract (PRE) by comparing phytochemical contents and biological activities according to extraction methods. high performance liquid chromatography (HPLC) analysis revealed that the contents of two major phytochemicals such as, tricin (5.35 mg/g) and p-coumaric acid (44.10 mg/g) contained in PRE were higher than those in fresh hot water extract (SQH, p-coumaric 23.39 mg/g, tricin 0.18 mg/g) and ethanol extract (SQE, p-coumaric 10.8 mg/g, tricin 0.38 mg/g). The antioxidant activities [1,1-diphenyl-2-picrylhydrazy (DPPH) radical scavenging activity, 2,2"-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical scavenging activity, nitric oxide (NO) scavenging activity, and xanthine oxidase inhibitory activity] of PRE were higher than those of SQH and SQE. PRE effectively inhibited NO production in LSP- stimulation RAW 264.7 cells, and the growth of human promyelocytic leukemia (HL-60) cells. These results suggest that PRE has a potential as a promising antioxidant and anti-inflammatory agent.

제주도 자생 제주아그배 (Malus micromalus Makino) 추출물의 항산화와 항염증 활성

이주엽(Ju-Yeop Lee),강민철(Min-Chul Kang),이정아(Jung-A Lee),고광효(Kwang-Hyo Ko),김봉석(Bong-Seok Kim),한종헌(Jong-Heon Han),김세재(Se Jae Kim),김기옥(Gi-Ok Kim) 한국생물공학회 2009 KSBB Journal Vol.24 No.4

본 연구는 제주에 자생하고 있는 제주아그배의 생리활성, 즉 항산화 효과와 항염 효과를 알아보고자 수행하였다. 제주 아그배의 항산화 활성을 측정하고자 비교 분석한 결과, 제주 아그배 에탄올 추출물 (DPPH 소거활성 IC50 값, 16.1 μg/mL; NO 소거활성 IC50 값, 901.2 μg/mL; superoxide 소거활성 및 Xanthine oxidase 억제 활성 IC50 값, 42.5 μg/mL, 2.7 μg/mL) 은 좋은 항산화 활성을 보였고, 그 분획물 중에서는 에틸아 세테이트 분획물이 항산화 활성 실험 모두에서 높은 소거 활성을 나타내었다. 따라서 항산화 활성이 좋은 제주아 그배의 추출물의 항염증 효과를 탐색하기 위하여 대식 세포 모델을 이용하였다. LPS로 자극한 마우스 대식세포인 RAW 264.7 세포에서 NO 활성과 세포독성간의 상대적 비교지수인 선택지수를 이용하여 탐색한 결과 에탄올 추출물과 에틸아세테이트 분획물이 2.9와 12.2로 가장 높은 상대적 NO 생성 억제 활성을 보였다. 그리고 NO 생성 억제율에 대한 IC50 값은 각각 16.7 μg/mL, 4.1 μg/mL으로 나타났다. 이런 결과를 기초로 하여 제주아그배의 에탄올 추출물의 NO 감소가 iNOS 단백질 감소에 의한 것임을 확인한 결과, 시료를 처리한 RAW 264.7 세포에서 iNOS 단백질이 농도 의존적으로 감소하였다. 또한 iNOS 단백질의 억제가 mRNA의 감소로 인한 것인지 조사하고자, RT-PCR 을 통해 iNOS 의 mRNA 발현을 조사한 결과, 단백질과 마찬가지로 농도 의존적인 mRNA 발현 감소를 볼 수 있었다. 제주아그배의 에탄올 추출물을 농도 별로 처리하였을 때 유의적으로 COX-2 단백질과 mRNA 발현이 농도 의존적으로 억제하였다. 분획물 중에서는 NO 활성과 세포 독성간의 상대적 비교지수인 선택지수가 높았던 에틸아세테 이트 분획물이 에탄올 추출물보다도 NO, iNOS, COX-2의 억제 정도가 더 좋은 경향을 나타내었다. The antioxidant and anti-inflammatory activities of ethanol extract of Malus micromalus were studied in vitro. Ethanol extract of M. micromalus showed scavenging effects on 1,1-diphenyl-2-picrylhydrazyl (DPPH) and nitric oxide (NO) radicals. In addition, ethanol extract of M. micromalus inhibited the generation of superoxide anion (O2 -) radical and uric acid by xanthine oxidase. We also investigated the effect of ethanol extract of M. micromalus on NO production in a lipopolysaccharide (LPS)-stimulated murine macrophage RAW 264.7 cells. Ethanol extract of M. micromalus significantly inhibited NO production and this inhibitory action was not due to the cytotoxicity. The expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) was markedly down-regulated by ethanol extract of M. micromalus. These results indicate that the inhibitory action of ethanol extract of M. micromalus on NO production in LPS-stimulated macropages might be due in part to abrogation of iNOS and COX-2 protein induction. Taken together, this study suggests that ethanol extract of M. micromalus could contribute to the chemoprevention and therapy of oxidative stress and inflammation.

포르말린과 청색 LED 병용처리에 의한 항스쿠티카 효과

강문경,이주엽,이윤항,김도형,Kang, Mun-Gyeong,Lee, Ju-Yeop,Lee, Yoonhang,Kim, Do-Hyung 한국어병학회 2022 한국어병학회지 Vol.35 No.1

Scuticociliatosis caused by Miamiensis avidus is a very important parasitic disease in olive flounder farming industry. The aim of this study was to determine effect of combined treatment with blue LED (light-emitting diode) illumination and formalin on olive flounder (Paralichthys olivaceus) infected with M. avidus. Different intensity of 405 nm LED (20, 40, and 60 μmol·m^-2·s^-1) was illuminated on 2.2×10⁴ cells/well of M. avidus in a 24 well microplate for 24 h. Also, 2.4×10⁴ cells/well of M. avidus were exposed to varying combinations of 60 μmol·m^-2·s^-1 of 405 nm LED and serial 10-fold dilutions of formalin (from 10 to 100 ppm) for 15, 30, 45, and 60 min. Surviving M. avidus were counted using a hemocytometer. For in vivo test, flounder acclimatized at 11-12 practical salinity unit (psu) were challenged with 2×10⁶ cells/ml of M. avidus by immersion method for 1 h. Then, fish were moved and divided into four groups; "F" group, treated with formalin at 50 ppm; "L" group, treated with 60 μmol·m^-2·s^-1 of 405 nm LED; "C" group, treated with combination of the two methods; and the control group. After treatment for 30 min, fish were transferred to new tanks (salinity = 11-12 psu) and observed for 3 weeks. As a result, illumination of 405 nm LED at 60 μmol·m^-2·s^-1 killed 100% of M. avidus after 12 h, while 67% and 90% of the scuticociliate died at 20 and 40 μmol·m^-2·s^-1, respectively, after 24 h exposure. One hundred percent of M. avidus was killed at 90, 80, 80 and 70 ppm after exposure to formalin for 15, 30, 45 and 60 min, respectively. However, combined method (e.g., 60 μmol·m^-2·s^-1 of 405 nm-LED plus 50 ppm formalin) killed the parasite within 30 min. From in vivo test, similarly, survival rates of fish challenged with M. avidus were 100%, 43%, 29% and 0% in the C, F, L, and control groups, respectively. Results obtained in this study demonstrates that the combined treatment method has clear synergistic effect on scuticociliatosis in fish.

다종재료를 이용한 경량차체 개발

배한일,박동호,이주엽,이문용,류성지,Bae, Han-Il,Park, Dong-Ho,Lee, Ju-Yeop,Lee, Mun-Yong,Ryu, Seong-Ji 재료연구소 2004 機械와 材料 Vol.16 No.4

제주도 자생 노루참나물 (Pimpinella komarovil) 추출물의 항산화 효과 및 멜라닌 억제 효과

강민철(Min-Chul Kang),이주엽(Ju-Yeop Lee),이정아(Jung-A Lee),한종헌(Jong-Heon Han),김봉석(Bong-Seok Kim),김기옥(Gi-Ok Kim) 한국생물공학회 2008 KSBB Journal Vol.23 No.1

당뇨병성 케톤산증의 회복 중에 발생한 Torsades de Pointes 유발 심정지 증례

김진모 ( Jinmo Kim ),이주엽 ( Ju Yeop Lee ),유원상 ( Won Sang Yoo ),이명용 ( Myung Yong Lee ),정현경 ( Hyun-kyung Chung ) 대한내과학회 2021 대한내과학회지 Vol.96 No.5

당뇨병성 케톤산증 환자는 QT 간격 연장, 그리고 이에 따른 Torsades de Pointes 발생 위험의 위험요소를 많이 내포하고 있으며 실제로 치명적인 부정맥인 Torsades de Pointes, 그리고 그에 따른 심정지를 경험하였기에 문헌 고찰과 함께 보고하는 바이다. Diabetic ketoacidosis (DKA) is an acute complication related to severe hyperglycemia. While the mortality rate for DKA is low with appropriate therapy, several complications may lead to deterioration of the clinical course. Here, we report a case of a 23-year-old patient with DKA who suffered from a rare but hemodynamically unstable cardiac arrhythmia, polymorphic ventricular tachycardia with prolonged QT interval, or Torsades de Pointes. During the recovery phase of DKA, three episodes of Torsades de Pointes suddenly occurred, and were recovered by immediate defibrillation. The patient did not have structural heart disease or a genetic predisposition. To the best of our knowledge, this is the first report of an adult with DKA complicated with QT prolongation related to Torsades de Points after correction of ketosis. To manage DKA, more attention may be needed on changes in the QT interval as well as risk factors for Torsades de Points. (Korean J Med 2021;96:432-437)

제주도 근해에 자생하는 참가죽그물바탕말 (Dictyota coriacea) 추출물의 멜라닌 억제 효과 및 항염증 효과

강민철(Min-Chul Kang),이주엽(Ju-Yeop Lee),고려경(Ryeo Kyeoung Ko),김행범(Haeng-Bum Kim),홍승호(Seung-Ho Hong),김기옥(Gi-Ok Kim) 한국생물공학회 2008 KSBB Journal Vol.23 No.4