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직장암에서 (99m)Tc-항CEA 항체 F(ab`)₂분절을 이용한 수술전 방사면역신티그라피 및 방사면역지침수술에 관한 기초연구
류진숙(Jin Sook Ryu),김진천(Jin Choen Kim),김창남(Chang Nam Kim),공경엽(Gyung Yub Gong),이희경(Lee Kyung Lee) 대한핵의학회 2000 핵의학 분자영상 Vol.34 No.3
N/A Purpose: This prospective study was performed to evaluate the usefulness of preoperative radioimmu-noscintigraphy and intraoperative scintimetric examination (radioimmunoguided surgery: RIGS) using (99m)Tc-anti-CEA F(ab')₂, fragment. Materials and Methods: Nineteen patients with rectal cancer underwent preoperative whole body planar scintigraphy at 4 hours after injection of (99m)Tc-anti-CEA F(ab')₂, fragment and SPECT imaging at 18 hours. Surgical operation was performed at 24 hours after injection. During laparotomy, radioactivities from intraabdominal viscera were measured by gamma probe. The radioac-tivities from excised tumor and lymph nodes were also measured and compared with pathology. Results: All nineteen patients were confirmed to have adenocarcinomas in the rectum. Twenty-seven of 97 excised lymph node groups had metastasis and 2 patients had liver metastasis in pathology. Preoperative radioim- munoscintigraphy detected primary tumors in 11 patients (sensitivity 55% ) and it couId not detect any lymph nodes or liver metastasis. All patients showed high radioactivity in the kidneys, liver, spleen, and major vessels in intraoperative measurement by gamma probe, and tumor activity was not discriminated from background activity. However, ra4ioactivity from excised tumor was higher than normal rectum (T/B ratio; 3.47±2.25). When excised lymph node activity/background activity ratio >1,5 was considered as positive criteria of metastasis, sensitivity, specificity, positive and negative predictive values were 78.6%, 73.9%, 55.0% and 89.5%, respectively. Conclusion: Radioimmunoscintigraphy using (99m)Tc-anti-CEA F(ab')₂. has no additional value for preoperative staging and use of early RIGS using (99m)Tc-anti-CEA F(ab')₂is inappropriate. For early RIGS using (99m)Tc labeled antibodies in rectal cancer patients, further development of more specific antibodies and methods to reduce background activity are needed. (Korean J Nucl Med 2000;34:243-51)
두경부 편평상피세포암종에서 $^{18}FDG-PET$을 이용한 방사선치료 반응평가
이상욱,류진숙,이병용,김종훈,안승도,신성수,김상윤,남순열,송시열,윤상민,박진홍,김성배,김재승,Lee Sang-Wook,Ryu Jin-Sook,Yi Byong-Yong,Kim Jong-Hoon,Ahn Seung-Do,Shin Seong-Soo,Kim Sang-Yoon,Nam Soon-Yuhl,Song Si-Yeol,Yoon Sang-Min,Park Jin-H 대한두경부종양학회 2003 대한두경부 종양학회지 Vol.19 No.1
Purpose: To evaluate the efficacy of positron emission tomography with 2-[F-18] fluoro-2-deoxy-D-glucose in discrimination of response in the nasopharyngeal carcinoma patients who treated with radiotherapy. Methods and Materials: Twenty-four patients who underwent FDG-PET scan before and after radiotherapy for no disseminated head and neck carcinoma at the Asan Medical Center between August 2001 and September 2002 were evaluate by prospective analysis. First FDG-PET scan performed before radiotherapy within 1 month, and second FDG-PET scan performed 1 month after radiotherapy. FDG-PET images were analyzed by standard uptake value (SUV). Follow-up period was more than 6 months. Results: The pretreatment SUV was 3.4-14.0 (median: 6.0) and posttreatment SUV was ground level-7.7 (median: 2.0). The overall sensitivity and specicity of FDG-PET to evaluate residual tumors in the nasopharyngeal carcinoma patients were 94% and 94%. Conclusion: FDG-PET is effective in evaluation of radiation response in the nasopharyngeal carcinoma. We think that the timing of one month after finished radiotherapy FDG-PET scan was not too fast to evaluation of radiation response.
역전사 중합효소 연쇄반응 ( RT-PCR ) 에 의한 HCV-RNA 의 검출 Biotin 및 방사성옥소 표지 Primer 로 구성된 Kit 의 이용
문대혁(Dae Hyuk Moon),류진숙(Jin Sook Ryu),이영상(Young Sang Lee),정영화(Young Hwa Chung),천준홍(Jun Hong Cheon),정윤영(Yoon Young Chung),박흥동(Hung Dong Park) 대한핵의학회 1994 핵의학 분자영상 Vol.28 No.2
N/A This study was performed to evaiuate the clinical applicability of the reverse t.ranscription polymerase chain reaction (RT-PCR) kit of HCV-RNA using biotinylated and radioiodinat.ed primers. Study subjects were 118 patients with positive anti-HCV. HCV-RNA in patient.s serurn was ext.racted by guanidium thiocyanate method. After first amplification, the product. was reamplified by primers labelled with biotin and I-125. The final amplification product was de- tected by counting t,he radioactivity after incubation in avidin coated tubes. In 51 samples, t.he test was repeated for evaluation of reproducibility. This new method was also compared with conventional RT-PCR rnethods in 34 samples from patients with chronic liver disease. The results were as follows, 1) HCV-RNA was positive in 85(97%)of 88 patients with chronic liver disease, and in 23 (73%) of 30 pat.ients with normal liver function. 2) In comparison with conventional method, HCV-RNA was detected in 32(94%) of 34 patient.s with new method, whereas in 27(79% ) of the same group with conventional method, 3) Repeated test with new rnet.hod in 52 samples demonstrat.ed 82% of concordant result. In conclusion, new rnethod with biotinylated and radioiodinated primers was rnore sensitive than conventional method. However, great care must be taken for quality control because there were considerable interassay variat,ion and possiblity of false positivity and false negativity.
저온온열치료에 의한 종양 내 저산소상태 개선효과를 18F-Fluoromisonidazole의 섭취 변화를 이용한 평가
이상욱(Sang-wook Lee),류진숙(Jin Sook Ryu),오승준(Seung Joon Oh),임기천(Ki Chun Im),천기정(Gi Jeong Chen),이소령(Ryung Lee),송도영(Do Young Song),임수정(Soo Jeong Im),문은숙(Eun Sook Moon),김종훈(Jong Hoon Kim),안승도(Seung Do Ahn),신 대한방사선종양학회 2004 Radiation Oncology Journal Vol.22 No.4
목 적: 종양 내에서 산소공급 부족현상으로 발생하는 저산소증 조직에서 저온온열치료(42oC)와 nicotinamide 에 의한 perfusion limited 저산소증의 개선 효과를 마우스 종양 모델을 이용하여 종양 내 [18F]FMISO 섭취변화 를 이용하여 증명할 수 있는지 알아보고자 하였다. 대상 및 방법: C3H 마우스에 [18F]FMISO를 정주하고 11개 장기에서 %ID/g을 구하여 biodistribution을 관찰하였다. 또한 같은 마우스에 동종 종양세포인 SCC7을 이식하여 종양모델을 만들고 저온온열치료(42oC)와 nicotinamide를 투여한 마우스와 대조군 마우스에서 [18F]FMISO의 섭취정도 차이를 %ID/g, autoradiography, PET scan을 시행하여 비교하고자 하였다. 결 과: 대조군에서 종양의 FMISO의 섭취는 5.1+/-2.28 %ID/g였고, 종양/근육, 종양/혈액의 섭취비는 2.2와 1.8이었다. 실험군에서는 각각 2.4+/-0.64 %ID/g, 1.4와 1.2를 나타내어 대조군보다 유의하게 낮았다(p<0.021). Autoradiography에서 대조군의 종양 내부에 FMISO가 섭취됨을 확인하였고, 저온온열치료와 nicotinamide를 투여한 실험군에서는 섭취가 감소된 것을 관찰하였다. 결 론: C3H 마우스와 동종 종양세포인 SCC-VII을 이용한 종양모델에서 [18F]FMISO가 종양내에 섭취가 되어 자산소증 종양모델로 적절함을 확인하였고, 저온온열치료(42oC)와 nicotinamide에 의한 perfusion limited 저산소증 개선효과를 [18F]FMISO의 종양 내 섭취가 감소하는 것을 통하여 확인할 수 있었다. Purpose: The aims of this study were to evaluate the change of [18F]fluoromisonidazole ([18F]FMISO) uptake in C3H mouse squamous cell carcinoma-VII (SCC-VII) treated with mild hyperthermia (42oC) and nicotinamide and to assess the biodistribution of the markers in normal tissues under similar conditions. Methods and Materials: [18F]FMISO was producedby our hospital. Female C3H mice with a C3H SCC-VII tumor grown on their extremities were used. Tumors were size matched. Non-anaesthetized, tumor-bearing mice underwent control or mild hyperthermia at 42oC for 60 min with nicotinamide (50 mg/kg i.p. injected) and were examined by gamma counter, autoradiography and animal PET scan 3 hours after tracer i.v. injected with breathing room air. The biodistribution of these agents were obtained at 3 h after [18F]FMISO injection. Blood, tumor, muscle, heart, lung, liver, kidney, brain, bone, spleen, and intestine were removed, counted for radioactivity and weighed. The tumor and liver were frozen and cut with a cryomicrotome into 10-μm sections. The spatial distribution of radioactivity from the tissue sections was determined with digital autoradiography. Results: The mild hyperthermia with nicotinamide treatment had only slight effects on the biodistribution of either marker in normal tissues. We observed that the whole tumor radioactivity uptake ratios were higher in the control mice than in the mild hyperthermia with nicotinamide treated mice for [18F]FMISO (1.56±1.03 vs. 0.67± 0.30; p=0.063). In addition, autoradiography and animal PET scan demonstrated that the area and intensity of [18F]FMISO uptake was significantly decreased. Conclusion: Mild hyperthermia and nicotinamide significantly improved tumor hypoxia using [18F]FMISO and this uptake reflected tumor hypoxic status.