Comparative analysis of bacterial community between organic and conventional farming soils under plastic film house cultivation

Jaekyeong Song(송재경),Hang-Yeon Weon(원항연),Byung-Yong Kim(김병용),Jae-Hyung Ahn(안재형),Yi-Seul Kim(김이슬),In-Cheol Park(박인철) 한국토양비료학회 2011 한국토양비료학회 학술발표회 초록집 Vol.2011 No.10

Comparative Analysis of Bacterial Community in Heavy Metal Contaminated and Noncontaminated Upland Soil

Jaekyeong Song(송재경),Byung-Yong Kim(김병용),Jae-Hyung Ahn(안재형),Hang-Yeon Weon(원항연) 한국토양비료학회 2012 한국토양비료학회 학술발표회 초록집 Vol.2012 No.6

목질섭식곤충의 장내 세균 다양성 분석 및 섬유소 분해균 탐색

최민영,안재형,송재경,김성현,배진우,원항연,Choi, Min-Young,Ahn, Jae-Hyung,Song, Jaekyeong,Kim, Seong-Hyun,Bae, Jin-Woo,Weon, Hang-Yeon 한국미생물학회 2015 미생물학회지 Vol.51 No.3

In this study, gut bacterial communities in xylophagous insects were analyzed using the pyrosequencing of 16S rRNA genes for their potential biotechnological applications in lignocelluloses degradation. The result showed that operational taxonomic units (OTUs), species richness and diversity index were higher in the hindgut than in the midgut of all insect samples analyzed. The dominant phyla or classes were Firmicutes (54.0%), Bacteroidetes (14.5%), ${\gamma}-Proteobacteria$ (12.3%) in all xylophagous insects except for Rhinotermitidae. The principal coordinates analysis (PCoA) showed that the bacterial community structure mostly clustered according to phylogeny of hosts rather than their habitats. In our study, the two carboxymethyl cellulose (CMC)-degrading isolates which showed the highest enzyme activity were most closely related to Bacillus toyonensis $BCT-7112^T$ and Lactococcus lactis subsp. hordniae $NCDO\;2181^T$, respectively. Cellulolytic enzyme activity analysis showed that ${\beta}-1,4-glucosidase$, ${\beta}-1,4-endoglucanase$ and ${\beta}-1,4-xylanase$ were higher in the hindgut of Cerambycidae. The results demonstrate that xylophagous insect guts harbor diverse gut bacteria, including valuable cellulolytic bacteria, which could be used for various biotechnological applications. 목질 섭식 곤충에 관한 장내 세균 군집의 연구를 이용한 lignocellulose의 분해는 생명 공학적 응용에 있어 큰 잠재력을 갖는다. 본 연구에서 목질 섭식 곤충의 장내 세균 군집은 16S rRNA 유전자를 기반으로 한 파이로시퀀싱 방법을 이용하여 분석되었다. 분석된 모든 곤충에서 중장보다 후장에서 OTU수, 종 풍부도, 다양성 지수가 높았다. 세균 문 또는 강 수준의 다양성을 분석한 결과, 흰개미를 제외한 곤충의 장내 군집에는 Firmicutes, Bacteroidetes, ${\gamma}-Proteobacteria$가 우점하였다. PCoA (principal coordinates analysis)를 이용하여 세균의 군집 구조를 분석한 결과, 서식지보다는 곤충의 과별로 클러스터링 되는 경향이었다. CMC 분해 활성이 가장 높은 두 균주는 Bacillus toyonensis $BCT-7112^T$와 Lactococcus lactis subsp. hordniae $NCDO\;2181^T$과 유연관계가 높았다. 장 적출물의 섬유소 분해활성 실험 결과, 하늘소 후장에서 ${\beta}-1,4-glucosidase$, ${\beta}-1,4-endoglucanase$, ${\beta}-1,4-xylanase$의 효소활성이 가장 높았다. 본 연구에서는 목질 섭식 곤충의 장내에 다양하고 풍부한 세균이 서식하며, 섬유소를 분해하는 세균이 존재한다는 사실을 확인하였고, 이로부터 다양하고 유용한 섬유소 분해균을 분리할 수 있을 것으로 판단되었다.

Streptomyces sp. A75와 A501 균주의 인삼 잘록병에 대한 방제효과

이상엽 ( Sang Yeob Lee ),송재경 ( Jaekyeong Song ),윤봉식 ( Bong-sik Yun ),박경훈 ( Kyeong Hun Park ),김정준 ( Jeong Jun Kim ),한지희 ( Ji Hee Han ) 한국균학회 2016 韓國菌學會誌 Vol.44 No.4

Streptomyces sp. A75 and A501 inhibited the mycelial growth of pathogenic Rhizoctonia solani and Pythium sp., which cause the ginseng disease known as damping-off. Three methods were evaluated for the control of these pathogens, using a mixture of the culture broths from Streptomyces sp. A75 and A501. The methods tested were seed dipping with 50-fold diluted broth, drenching of soil with 100-fold diluted broth after sowing, and combined seed dipping and drenching. These methods reduced the incidence of ginseng damping-off caused by R. solani by 81.3%, 84.8%, and 32.2% and that caused by Pythium sp. by 51.0%, 52.1%, and 75.3%, respectively. Based on these results, the combination of seed dipping and soil drenching after sowing using a mixture of the culture broths from Streptomyces sp. A75 and A501 effectively reduced the incidence of dampingoff in ginseng.

토마토에 염류 내성을 유도하는 바실러스 균주 처리 후 근권 미생물 군집 구조 연구

유성제,이신애,원항연,송재경,상미경,Yoo, Sung-Je,Lee, Shin Ae,Weon, Hang-Yeon,Song, Jaekyeong,Sang, Mee Kyung 한국환경농학회 2021 한국환경농학회지 Vol.40 No.1

BACKGROUND: Soil salinity causes reduction of crop productivity. Rhizosphere microbes have metabolic capabilities and ability to adaptation of plants to biotic and abiotic stresses. Plant growth-promoting bacteria (PGPB) could play a role as elicitors for inducing tolerance to stresses in plants by affecting resident microorganism in soil. This study was conducted to demonstrate the effect of selected strains on rhizosphere microbial community under salinity stress. METHODS AND RESULTS: The experiments were conducted in tomato plants in pots containing field soil. Bacterial suspension was inoculated into three-week-old tomato plants, one week after inoculation, and -1,000 kPa-balanced salinity stress was imposed. The physiological and biochemical attributes of plant under salt stress were monitored by evaluating pigment, malondialdehyde (MDA), proline, soil pH, electrical conductivity (EC) and ion concentrations. To demonstrate the effect of selected Bacillus strains on rhizosphere microbial community, soil microbial diversity and abundance were evaluated with Illumina MiSeq sequencing, and primer sets of 341F/805R and ITS3/ITS4 were used for bacterial and fungal communities, respectively. As a result, when the bacterial strains were inoculated and then salinity stress was imposed, the inoculation decreases the stress susceptibility including reduction in lipid peroxidation, enhanced pigmentation and proline accumulation which subsequently resulted in better plant growth. However, bacterial inoculations did not affect diversity (observed OTUs, ACE, Chao1 and Shannon) and structure (principle coordinate analysis) of microbial communities under salinity stress. Furthermore, relative abundance in microbial communities had no significant difference between bacterial treated- and untreated-soils under salinity stress. CONCLUSION: Inoculation of Bacillus strains could affect plant responses and soil pH of tomato plants under salinity stress, whereas microbial diversity and abundance had no significant difference by the bacterial treatments. These findings demonstrated that Bacillus strains could alleviate plant's salinity damages by regulating pigments, proline, and MDA contents without significant changes of microbial community in tomato plants, and can be used as effective biostimulators against salinity stress for sustainable agriculture.

Bacillus velezensis CC112 균주의 인삼 잘록병에 대한 생물적 방제

이상엽 ( Sang Yeob Lee ),송재경 ( Jaekyeong Song ),박경훈 ( Kyeong Hun Park ),원항연 ( Hang Yeon Weon ),김정준 ( Jeong Jun Kim ),한지희 ( Ji Hee Han ) 한국균학회 2016 韓國菌學會誌 Vol.44 No.3

Bacillusvelezensis CC112 균주는 인삼 잘록병을 일으키는 Rhizoctonia solani를 비롯한 여러 식물병원균의 균사 생장을 억제하였다. 인삼 잘록병균 R. solani에 대하여 B. velezensisCC112 균주를 Luria-Bertani (LB)와 Bacillus-soytonemedium 배지에 배양한 10배 희석액의 종자침지처리와 LB배지에 배양한 10배 희석액을 토양관주처리하여 65.8%, 67.1%와 64.2% 방제효과를 나타냈다. B.velezensis CC112균주의 시제품을 100배로 희석하여 토양관주처리하여 R.solani에 77.3%, Pythium sp.에 65.7% 방제효과를 각각 나타내었다. 이들 결과에서 B. velezensis CC112 균주는 인삼잘록병의 방제에 유망한 미생물살균제가 될 수 있다. Bacillus velezensis CC112 inhibited the mycelial growth of several plant pathogens, including Rhizoctonia solani, causing damping-off on ginseng. The control efficacies of B. velezensis CC112 against R. solani by seed dipping in LB and BSM broth diluted 10 times, soil dipping, and soil drenching with LB broth diluted 10 times were 65.8%, 67.1%, and 64.2%, respectively. Treatment of soil drenching with the 100 times diluted prototype of B. velezensis CC112 against R. solani and Pythium sp. by soil revealed control efficacies of 77.3% and 65.7%, respectively. These results indicate that B. velezensis CC112 is a prospective biofungicide for the biological control of ginseng damping off.

장수풍뎅이 유충의 분변에 존재하는 방선균의 다양성 및 항균활성

이혜원,안재형,김민욱,원항연,송재경,이성재,김병용,Lee, Hye-Won,Ahn, Jae-Hyung,Kim, Minwook,Weon, Hang-Yeon,Song, Jaekyeong,Lee, Sung-Jae,Kim, Byung-Yong 한국미생물학회 2013 미생물학회지 Vol.49 No.2

방선균은 의약, 농업 및 식품 생산 등에 유용하게 사용되는 이차대사물질을 생산하는 미생물 자원으로 자연환경 내에서 많은 생물들과 긴밀한 상호작용을 유지하고 있다. 본 연구에서는 장수풍뎅이 유충의 분변에 존재하는 방선균의 다양성과 기능성을 조사하기 위해서 비배양적 접근과 배양적 방법으로 실험을 수행하였다. 먼저 시료로부터 직접 추출한 community DNA에서 방선균-특이 primer를 이용하여 방선균의 16S rRNA 유전자를 PCR로 증폭, 클로닝한 후에 각 clone에 삽입된 염기서열을 분석하였다. 총 37개의 염기서열을 얻었으며 계통분류학적 분석을 수행한 결과, 15속 24종으로 분류되었다. 아울러 53개의 방선균 균주를 장수풍뎅이 유충 분변으로부터 분리하였다. 형태학적 특징을 비교하여 최종적으로 27개의 균주를 선발하여 다양성, 항균활성 및 생화학적 특징을 검정하였다. 분리된 균주들은 4속 14종으로 분류되었으며, 24균주(89%)는 Streptomyces 속으로 분류되었다. 대다수의 균주들이 식물병원성 곰팡이와 그람양성 세균에 대해 길항 효과를 보였다. 또한 많은 균주들이 셀룰로오스와 카제인을 분해하는 생화학적 특징을 보였다. 본 연구를 통해, 장수풍뎅이 유충의 분변 시료로부터 다양한 방선균이 분리될 수 있으며, 분리된 균주들은 다양한 항균효과 및 효소활성을 지니고 있다는 것을 확인하였다. 결론적으로 본 연구는 장수풍뎅이와 같은 곤충의 장과 분변은 다양한 방선균의 서식처이며, 이곳에서 유용한 생리활성 물질을 발견할 수 있다는 것을 제시한다. Actinomycetes produce diverse secondary metabolites which have the primary importance in medicine, agriculture and food production, and key to this is their ability to interact with other organisms in natural habitats. In this study, we have investigated the taxonomical and functional diversity of actinomycetes in fecal sample of rhinoceros beetle larvae (Allomyrina dichotoma L.) by using culture-dependent and -independent approaches. For the culture-independent approach, the community DNA was extracted from the sample and 16S rRNA genes of actinomycetes were amplified using actinomycetes-specific PCR primers. Thirty-seven clones were classified into 15 genera and 24 species of actinomycetes. For the culture-dependent approach, 53 strains were isolated from larval feces, of which 27 isolates were selected based on morphological characteristics. The isolates were classified into 4 genera and 14 species, and 24 isolates (89%) were identified as the genus Streptomyces. Many of the representative isolates had antimicrobial activities against plant pathogenic fungi and Gram-positive bacteria. In addition, most of the isolates (78%) showed biochemical properties to hydrolyze cellulose and casein. The results demonstrated that diverse and valuable actinomycetes could be isolated from insect fecal samples, indicating that insect guts can be rich sources for novel bioactive compounds.

Amycolatopsis sp. KCTC 29142로부터 유래된 siderochelin A의 다제 내성 균주에 대한 항균활성

이동령,성금화,이성권,홍희전,송재경,양승환,서주원,Lee, Dong-Ryung,Cheng, Jinhua,Lee, Sung-Kwon,Hong, Hee-Jeon,Song, Jaekyeong,Yang, Seung Hwan,Suh, Joo-Won The Microbiological Society of Korea 2016 미생물학회지 Vol.52 No.3

본 연구에서는 신규 Amycolatopsis 균주 KCTC 29142를 분리하여 형태학적 관찰, 계통분석 및 화학분류학적 분석 등 다상 분류분석을 통해 분석하였다. KCTC 29142 균주의 에틸아세테이트추출물은 강한 항균활성을 나타났고, 활성물질은 철 이온 킬레이트 물질인 siderochelin A로 동정되었다. 본 연구에서 분리된 siderochelin A는 다제내성균인 Acinetobacter baumanii, methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant Staphylococcus aureus (VRSA), 및 Escherichia coli (E. coli)에 대해 강한 활성을 보였고, 임상에서 분리된 다제내성균에 대한 MIC를 결정하였다. A novel Amycolatopsis strain KCTC 29142 was isolated and characterized based on the polyphasic taxonomic analysis including morphological observation, phylogenetic analysis, physiological and chemotaxonomic characteristics. The ethyl acetate extract of strain KCTC 29142 culture broth showed strong antibacterial activity and the active compound was identified as siderochelin A, a ferrous-ion chelating compound. In this study, siderochelin A showed good activity against multi-drug resistant pathogens, including Acinetobacter baumanii, methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant Staphylococcus aureus (VRSA), and Escherichia coli (E. coli). The minimum inhibitory activity against clinical isolates was also determined.

토마토 뿌리에서 분리한 식물생육촉진 세균 Bacillus velezensis T20E-257균주의 유전체 염기서열

이신애,김상윤,상미경,송재경,원항연,Lee, Shin Ae,Kim, Sang Yoon,Sang, Mee Kyung,Song, Jaekyeong,Weon, Hang-Yeon 한국미생물학회 2017 미생물학회지 Vol.53 No.4

Bacillus velezensis T20E-257 was isolated from the root tissue of a tomato plant and exhibited plant growth-promoting activity. Here we present the complete genome of strain T20E-257. The genome contains 3,900,066 base pairs with a G + C content of 46.7% in 2 contigs. The genome includes 3,708 coding sequences, 27 rRNAs, and 86 tRNAs. We found gene clusters encoding secondary metabolites with an antimicrobial activity and genes related to the production of indole-3-acetic acid and 2,3-butanediol, which play a role in plant growth and health. 토마토 뿌리에서 분리한 Bacillus velezensis T20E-257 균주는 식물촉진효과가 있었고, 본 연구에서 T20E-257 균주의 유전체 서열을 해독하였다. 유전체 초안에서 포함된 2개 contig는 총 염기서열이 3,900,066 bp고, G + C content가 46.7%이었다. 유전체에서 단백질 유전자 3,708개, rRNA 유전자 27개, tRNA 유전자 86개를 확인하였다. 항균활성을 가지는 2차 대사산물 생합성 관련 유전자군과 식물생육촉진에 관여하는 IAA와 2,3-butadiol 생합성 관련 유전자를 T20E- 257 균주 유전체에서 확인하였다.

Complete genome sequence of Variovorax sp. PMC12, a plant growth-promoting bacterium conferring multiple stress resistance in plants

이신애,김현수,김이슬,상미경,송재경,원항연,Lee, Shin Ae,Kim, Hyeon Su,Kim, Yiseul,Sang, Mee Kyung,Song, Jaekyeong,Weon, Hang-Yeon The Microbiological Society of Korea 2018 미생물학회지 Vol.54 No.4

본 연구에서 생물 및 비생물학적 스트레스에 내성을 유도하는 식물 생육 촉진 세균인 Variovarx sp. PMC12 균주의 유전체 염기서열을 분석하였다. PMC12 균주의 유전체는 5,873,297 bp와 1,141,940 bp 크기의 원형 염색체 2개로 구성되었다. 총 6,436개 단백질 유전자, rRNA 9개, tRNA 64개, ncRNA 3개와 유사유전자 80개가 확인되었다. 유전체상에서 발견된 1-aminocyclopropane-1-carboxylate (ACC) deaminase, 항산화 활성, 인산 가용화, 프롤린 생합성, 시드로포어 생합성과 관련된 유전자들은 PMC12 균주가 염, 온도, 병원균에 대한 스트레스에 대한 식물의 내성 유도와 관련되어 있을 것으로 판단된다. Variovorax sp. PMC12 is a rhizobacterium isolated from tomato rhizosphere and enhanced the plant resistance to abiotic and biotic stresses. Here we present the complete genome sequence of strain PMC12. The genome is comprised of two circular chromosomes harboring 5,873,297 bp and 1,141,940 bp, respectively. A total of 6,436 protein-coding genes, 9 rRNAs, 64 tRNAs, 3 ncRNAs, and 80 pseudogenes were identified. We found genes involved in 1-aminocyclopropane-1-carboxylate (ACC) deaminase, antioxidant activity, phosphate solubilization, and biosynthesis of proline and siderophore. Those genes may be related to capability of improving plant resistance to various stresses including salinity, cold temperature, and phytopathogen.