정상돈과 설사돈에서 분리한 대장균의 항균제 내성 및 Bla_TEM 분포 조사

변재원,김하영,정병열,배유찬,이완규,Byun, Jae Won,Kim, Ha Young,Jung, Byeong Yeal,Bae, You Chan,Lee, Wan Kyu 대한수의학회 2012 大韓獸醫學會誌 Vol.52 No.2

Antimicrobial resistance is one of the most concerns in pig industry. Escherichia (E.) coli have been used for the indicator to monitor the antimicrobial resistance. In this study, 321 E. coli from diarrheic and non-diarrheic piglets were tested for antimicrobial resistance and frequency of $Bla_{TEM}$. In non-diarrheic piglets, they were resistant to oxytetracycline (93%), streptomycin (92%) and sulfadiazine (90%) but susceptible to ceftiofur (99%), colistin (97%), and enrofloxacin (82%). The isolates from diarrheic piglets were resistant to enrofloxacin (72.9%), ceftiofur (17.6%), and colistin (11.3%), whereas the resistance was 1%, 18% and 3% in case of non-diarrheic piglets, respectively. The resistance for amoxicillin/clavulanic acid (54.1%) and ceftiofur (22%) was high in isolates from post-weaning piglets. The resistance for colistin was 15.2% in nursery piglets. Seventy-three percent of isolates from diarrheic piglets showed high multidrug resistance profile (more than 13 antimicrobials) compared to those from non-diarrheic pigs in which 71% of isolates showed moderate multidrug resistance profile (7 to 12 antimicrobials). The frequency of $Bla_{TEM}$ in E. coli from non-diarrheic and diarrheic piglets was 57% and 69%, respectively. The results might provide the basic knowledge to establish the strategies for treatment and reduce antibiotic resistance of E. coli in piglets.

양돈용 생균제 개발을 위한 유산균주 선발

변재원,김경태,배형석,백영진,이완규,Byun, Jae-won,Kim, Gyung-tae,Bae, Hyoung-suk,Baek, Voung-jin,Lee, Wan-kyu 대한수의학회 2000 大韓獸醫學會誌 Vol.40 No.4

This study was carried out to select the lactic acid bacteria(Lactobacillus, Streptococcus and Bifidobacterium) and yeast for probiotic use in pigs. Acid-tolerant 536 strains were isolated from the feces of 30 pigs. To select useful strains, the first screened strains were treated with strong acid solution(pH 2.5 to 3.0) for 3 hours and subsequentely treated with the anaerobic diluent solution containing 0.15% Oxgall for 3 hours. Among these strains, 151 strains showed strong tolerance to both acid and bile. Lactobacillus and Streptococcus tolerant to the acid and bile were treated with heat at $80^{\circ}C$ for 15 min, and at $70^{\circ}C$ for 5 min in Bifidobacterium and yeast. As a result of heat treatment, 38 strains were obtained as heat-tolerant strains. All of heat-tolerant strains were tested for antibiotic resistance against virginiamycin, sulfathiazole, aureomycin, neomycin, linsmycin, tiamulin and ASP250 which were used as feed additives for growth promotion in pigs. Finally, one strain each from Lactobacillus, Streptococcus, Bifidobacterium and yeast that showed resistance to acid, bile, heat and antibiotics was selected for probiotic use in pigs.

국내 유기견의 심장사상충 감염 실태 조사

변재원 ( Jae Won Byun ),박영일 ( Yong Il Park ),이오수 ( O Soo Lee ),심항섭 ( Hang Sub Shim ),조기만 ( Ki Man Cho ),윤순식 ( Soon Seek Yoon ) 한국수의공중보건학회 2007 예방수의학회지 Vol.31 No.1

Peripheal Neuropathy를 동반한 Microscopic Polyangiitis

변재원 ( Jai Won Byun ),이인홍 ( In Hong Lee ),정자헌 ( Ja Hun Jung ),김기찬 ( Ki Chan Kim ),김태환 ( Tae Hwan Kim ),전재범 ( Jae Bum Jun ),정성수 ( Sung Soo Jung ),배상철 ( Sang Cheol Bae ),유대현 ( Dae Hyun Yoo ),김호중 ( Ho Jun 대한류마티스학회 2000 대한류마티스학회지 Vol.7 No.2

Microscopic polyangiitis (MPA) is a systemic necrotizing vasculitis of small-sized vessels (capillary, venule, or arterioles) whose clinical manifestations are very similar to those of PAN (polyarteritis nodosa), but it is characterized by the presence of rapidly progressive glomerulonephritis (RPGN), which is nearly constant, and pulmonary involvement usually absent in PAN. Peripheral neuropathy occurs in only 14~36% of the patients with MPA, distinctly less frequent than in those with classic PAN. While PAN may be considered a self-limiting disease which tend not to recur once remission is acquired, relapse occurs frequently in MPA. Prognosis has been transformed by corticosteroids and immunosuppressive drugs, especially cyclophosphamide. We reported a 72-year-old woman with subacute sensorimotor polyneuropathy involving peroneal and sural nerve accompanied by focal segmental glomerulonephritis which was proven by renal biopsy.

도축우에서 Mannheimia haemolytica의 분리 및 항생제 감수성검사

변재원 ( Jae Won Byun ),김경호 ( Kyoung Ho Kim ),이승환 ( Seoung Whan Lee ),이혜화 ( Hye Wha Lee ),이정인 ( Jung In Lee ),황현순 ( Hyun Soon Hwang ) 한국가축위생학회 2003 韓國家畜衛生學會誌 Vol.26 No.2

This study was carried out to identify and investigate antimicrobial susceptibility for Mannheimuia haemolytica which is responsible for shipping fever. Samples were collected from nasal and lung of 100 adult healthy cattle which are slaughtered in Samsung meat corporation located in Incheon metropolitan city. Lung lesion index have been investigated within 0-5 range according to Shewen and Wilkie(Can J Vet Res 52 30-36, 1988). Eighty-seven of 100 cattle were under normal condition with 0-1 ranges. A total of 129 strains were collected from blood and tryptic soy agar. Among these strains, 100 strains were identified with Staphylococcus, Streptococcus and enterobacteria containing E coil. Biochemical and fermentation assay of arabinose, trehalose, xylose, mannose, mannitol, lactose and salicin were tested to identify with Mannheimia sp. for 7 strains shown haemolytic activity on blood agar. Five strains were identified with Mannheimia Iriemolytiai and 2 strains were untyped. In seasonal survey, Mannheimia sp recovered from fall to winter(5 of 7) have been highly isolated rather than those from spring to sumrner(2 of 7). Mannheimia izemoiytkv were susceptible to antibacterials tested in this study but more resistant to oxytetracycline and streptomycin.

초기 말더듬아동의 비유창성 특성 연구

변재원(Jae Won Byun),이은주(Eun Ju Lee),심현섭(Hyun Sub Sim) 한국언어청각임상학회 2004 Communication Sciences and Disorders Vol.9 No.1

본 연구에서는 초기 말더듬아동과 일반아동의 발화에 나타난 비유창성의 유형과 빈도, 총비유창성에 대한 진성비유창성(SLD)과 가성비유창성(OD)의 비율, 말더듬 정도, 평균반복단위 등을 비교하였다. 또한 가중말더듬지수를 기초로 초기 말더듬아동의 말더듬 정도를 알아보고, 말더듬 경과기간이 지남에 따라 비유창성 측정치들이 어떻게 변화되어 가는지 살펴보았다. 연구대상은 말더듬이 시작된 지 6개월 이내에 있는 초기 말더듬아동 10명과 이들과 성별, 생활연령을 일치시킨 일반아동 10명이었다. 연구결과, 총비유창성의 빈도, SLD의 빈도, 가중말더듬지수와 평균반복단위에 있어서 두 집단은 유의한 차이를 보였다. 또한 총비유창성에 대한 SLD와 OD의 비율에 집단간 차이를 보여 SLD가 말더듬 진단시 집단을 변별하기 위한 준거로 사용될 수 있음을 보여주었다. 가중말더듬지수를 근거로 한 말더듬 정도 평가에서 초기 말더듬아동의 말더듬 정도는 약함보다는 중간이나 심한 정도인 것으로 나타났다. 상관분석을 통해 초기 말더듬아동의 말더듬 경과기간과 비유창성 측정치들의 상관을 살펴본 결과, 유의한 상관관계를 발견하지 못하였다. 이는 초기 말더듬은 그 시작시기부터 일반아동과 큰 차이를 보이고 상대적으로 심한 정도의 비유창성을 보일 수 있다는 최근 연구결과와 일치하는 것이다. The purpose of the present study was to investigate the differences in speech disfluencies between normal and stuttering children and the relation of TSO(time since onset of stuttering) to 5 measures of stuttering behaviors for stuttering children. Ten stuttering children aged 2 to 7 who are within 6 months since the onset of stuttering and 10 gender- and age-matched normally fluent children participated in this study. Speech samples, collected for each subject were at least 200 syllables in length. All utterances during the task were video-recorded and transcribed for analysis. The Mann-Whitney U test was used to examine group differences and Spearman correlation coefficient was also calculated to examine the relationship between TSO and the 5 dependent measures of disfluencies. The results obtained were as follows: (1) mean number of SLD, mean number of repetition units, and weighted SLD scores significantly differed between the two groups, whereas the mean number of OD did not show a significance difference between the two groups. Also, the proportion of each type(SLD, OD) in the total disfluencies differentiated the two groups. (2) The two groups differed from each other in terms of weighted SLD scores. The stuttering severity of children near the stuttering onset was moderate to severe, rather than mild. (3) Within the stuttering children no significant relationship was found between TSO and any of the 5 measures of stuttering. The findings of the present study were taken to suggest that certain measures of speech disfluencies, such as weighted SLD scores and proportions of disfluencies, can serve as an important diagnostic tool for differentiating stuttering children and normally fluent children.

사료 첨가 항생제 금지 전후 돼지 설사증 유래 대장균의 병원성 인자 및 항생제 내성 유전자

도경효,변재원,이완규,Do, Kyung-Hyo,Byun, Jae-Won,Lee, Wan-Kyu 대한수의학회 2020 大韓獸醫學會誌 Vol.60 No.3

This study examined the prevalence of adherence factors, toxin genes, antimicrobial resistance phenotypes, and resistance genes in Escherichia coli (E. coli) isolated from piglets with diarrhea before and after the ban on antibiotic growth promoters (AGPs) in Korea from 2007 to 2018. In this period, pathogenic 474 E. coli isolates were obtained from diarrheic piglets. The virulence factors and antimicrobial resistance genes were assayed using a polymerase chain reaction, and the susceptibility to antibiotics was tested according to the Clinical and Laboratory Standards Institute guidelines. After the ban on AGPs, the frequency of F4 (12.5% to 32.7%) increased significantly, and LT (31.9% to 20.3%) and EAST-I (46.5% to 35.2%) decreased significantly. In addition, the resistance to streptomycin (45.8% to 67.9%), cephalothin (34.0% to 59.4%), and cefazlin (10.4% to 28.8%) increased significantly. Colistin resistance plasmid-mediated genes, mcr-1 and mcr-3, were detected after the ban on AGPs. The results of this study can provide useful data for analyzing the impact of the ban on AGPs on the virulence profiles and antimicrobial resistance of E. coli isolated from piglets with diarrhea in Korea.

국내 유기견의 개 옴 감염 상황

윤순식,변재원,양동군,신연경,위성환,김병한,Yoon, Soon-Seek,Byun, Jae-Won,Yang, Dong-Kun,Shin, Yeun-Kyung,Wee, Sung-Hwan,Kim, Byounghan 대한수의학회 2010 大韓獸醫學會誌 Vol.50 No.3

Recently, the number of stray dogs is proportionate to the increase of the number of the companion dogs. Sarcoptic scabiei var canis, causing scabies, is one of the most important canine zoonotic arthropods in Korea and around the world. Thus, we have tried to know the prevalence of canine scabies in the stray dogs in Korea. A total of 565 stray dogs were collected from the rescue centers all over the country from Jan. 2006 to Dec. 2006. They were grouped with euthanasia or natural death and examined for the gender and age estimated by dental formula. To identify the lesions, the whole body was grossly examined and tested pathologically. Thirty two (5.66%) of 565 dogs were diagnosed as canine scabies. Dogs from urban areas had fewer scabies (0.62%) than those of rural areas (12.5%). Prevalence of scabies in male and female dogs was no difference as 5.96% and 5.25%. Euthanasia group showed higher prevalence (6.48%) than natural death group (2.44%) in scabies. Old dogs over five years showed lower infestration (1.82%) in scabies. In histopathological findings, there were mites in the burrows formed in the subcorneal space. Acanthosis, hyperkeratosis with crust, and vascular dilatation were main findings. One hundred thirteen (20%) of 565 stray dogs were diagnosed to have skin disease. Among them, canine scabies is the most prominent ectoparasite as 5.66 %. With previous reports on human infection in Korea, canine scabies must be regarded as the important zoonotic canine skin disease. Accordingly, for the human and canine hygiene it is imperative that stray dogs with skin problems are segregated and tested for the parasites to treat properly as soon as arriving at rescue shelter.

돼지 희석정액의 세균오염도 및 유효 항생제 선발

김하영,변재원,신동호,김형순,윤하정,박최규,이오수,정병열,Kim, Ha-Young,Byun, Jae-Won,Shin, Dong-Ho,Kim, Hyoung-Soon,Yoon, Hachung,Park, Choi-Kyu,Lee, O-Soo,Jung, Byeong Yeal 대한수의학회 2010 大韓獸醫學會誌 Vol.50 No.2

Bacterial contamination is an unavoidable finding of the semen collection process in boar and can lead in deleterious effects on semen quality and longevity if left uncontrolled. The purpose of this study is to identify the bacteria in extended boar semen and to select the effective antimicrobials to control of the contaminants. Of 116 extended boar semen samples submitted from eight AI centers in Korea, 39 (33.6%) samples were positive for bacterial contamination. Among 39 contaminated semen, most of them (84.6%) were contaminated with one or two bacterial species and there was no significant difference between two age groups $(\leq\;24\;and\;>\;24\;month\;old).$ Stenotrophomonas maltophilia (n = 18) was the most predominant bacterium followed by Elizabethkingia meningoseptica (n = 12), Sphingomonas paucimobilis (n = 12), Myroides spp. (n = 5), Ochrobactrum anthropi (n = 3), and so on. Enrofloxacin (72.9%), florfenicol (72.9%), bacitracin (49.2%) and tylosin (49.2%) showed higher sensitivity compared with penicillin (13.6%) or aminoglycosides (6.8%-18.6%). Brucella spp., Leptospira spp., Mycoplasma hyopneumoniae, Mycoplasma hyorhinis, Mycobacterium tuberculosis complex were not detected in semen by PCR.

엘크의 구포자충 감염사례

윤순식,배유찬,변재원,이경현,장환,송재영,위성환,Yoon, Soon-Seek,Bae, You-Chan,Byun, Jae-Won,Lee, Kyeong-Hyun,Jang, Hwan,Song, Jae-Young,Wee, Sung-Hwan 대한수의학회 2010 大韓獸醫學會誌 Vol.50 No.2

A thirty-five week old elk was referred for Chronic Wasting Disease (CWD) confirmation from Chungnam province in Korea. Necropsy revealed much bloody contents in the lumen of small and large intestines, and reddening of its mucosa. Microscopically, few coccidia showing various developmental stages were infiltrated in lamina propria of small intestine with granulomatous inflammation and congestion. They were identified as Eimeria spp. according to their location and morphological characteristics. Because fecal examination and oocyst culture were not available, the species of the Eimeria could not be confirmed. There were no detection of pathogenic bacteria such as clostridia and virus in intestinal contents. CWD was shown to be negative in immunohistochemistry test. In conclusion, it was the first Eimeria case detected in small intestine of Korean domestic elk as far as we know although Eimeria spp. were not the main cause of death.