한국 폐경 여성에서 부갑상선 호르몬, 비타민 D와 골대사 사이의 관계

소재성 ( So Jae Seong ),박형무 ( Park Hyeong Mu ) 대한산부인과학회 2004 Obstetrics & Gynecology Science Vol.47 No.1

목적 : 본 연구는 한국 폐경 여성에서 부갑상선 호르몬, 비타민 D 상태, 골밀도와 골표지 물질 사이의 상관관계를 알아보고자 하였다. 연구 방법 : 본 연구는 1999년 1월부터 1999년 3월까지 서울시내 거주하는 263명의 건강한 폐경 여성을 대상으로 하였다. 부갑상선 호르몬 농도는 intact PTH IRMA kit를 이용하여 면역방사측정법 (immunoradiometric assay, IRMA)으로 측정하였다. 비타민 D 영양상태를 알아보기 위해 Objective : The purpose of this study was to evaluate the relationship between parathyroid hormone (PTH), vitamin D status, bone mineral density (BMD) and bone turnover markers in Korean postmenopausal women. Methods : The subjects were 263 healthy postme

전기포트와 가습기 기능을 가지는 가열기기 구현

소재성(Jae-seong So),박진영(Jin-young Park),송지환(Ji-hwan Song),고다인(Da-in Go),정재훈(Jae-hun Jang),최철웅(Cheol-woong Choi),임민희(Min-Hee Lim),배희재(Hui-Jae Bae),소재현(Jae-hyeon So),김대경(Dae-kyong Kim) 대한전기학회 2019 대한전기학회 학술대회 논문집 Vol.2019 No.10

스트론튬 격리화를 위한 요소 분해 박테리아의 분리 및 특성 연구

최재호(Jae-Ho Choi),강창호(Chang-Ho Kang),한상현(Sang-Hyun Han),곽대영(Dae Young Kwak),오수지(SooJi Oh),소재성(Jae-Seong So) 한국생물공학회 2013 KSBB Journal Vol.28 No.3

In this study, 52 ureolytic bacterial strains were newly isolated from various environments. From these, 2 strains (TB-15 and TB-22) were selected based on their high urease activity. XRD spectra clearly showed presence of various sequestration products such as calcite and strontianite in samples. TB-22 showed 20~30% higher survivability upon Sr concentration (20 mM) than Sporosarcina pasteurii KCTC 3558. TB-15 and TB-22 showed 80~90% higher survivability at pH 6 than S. pasteurii. The results demonstrated that the 2 isolates colud be good candidates for the bioremediation of Sr contaminated sites.

가임기 여성의 질에서 분리한 젖산 세균인 Lactobacillus plantarum UK-3의 특성 및 항균활성

안혜란,소재성,오계헌,Ahn, Hye-Ran,So, Jae-Seong,Oh, Kye-Heon 한국미생물학회 2011 미생물학회지 Vol.47 No.4

본 연구는 가임기 여성의 질로부터 젖산균인 Lactobacillus plantarum UK-3를 분리하여 다양한 생리화학적 특성조사와 여러 가지 병원균들에 대한 항균활성을 확인하고자 실시하였다. 균주 UK-3는 MRS 배지에서 배양되었으며, 형태학적 관찰 및 생화학적 특성을 조사하였으며, 16S rRNA 염기서열 분석을 통해 균주를 동정하여, Lactobacillus plantarum UK-3로 명명하였으며, GenBank에 [JK266589]로 등록하였다. 배양기간 동안에 L. plantarum UK-3의 생장과 유기산의 생성, pH 변화를 조사하였으며, HPLC를 사용하여 대사산물로서 생성된 젖산(lactic acid)과 아세트산(acetic acid)을 정량 및 정성분석 하였다. 이들 유기산의 생성은 L. plantarum UK-3의 생장과 비례하였고, 배양 48시간 경과 후에 젖산과 아세트산의 농도는 각각 약 684.11 mM과 174.26 mM이었으며, 초기 pH 7.0은 배양기간 동안 3.7로 감소하였다. 여성의 질에서 감염의 가능성이 있는 10가지의 그람양성 세균, 그람음성 세균, 그리고 효모에 대하여 25배로 농축된 배양상등액을 처리하여 plate diffusion assay 방법으로 항균활성을 측정하였다. 그 결과 본 연구에 사용된 10가지 미생물에 대해서 광범위하게 항균효과가 있는 것이 관찰되었으며, 정상균총으로서 다른 젖산세균인 Lactobacillus acidophilus에 대해서는 항균활성을 나타내지 않았다. The purpose of this work was to examine the antimicrobial activity derived from the lactic acid bacterium, UK-3 isolated from the vaginas of women of childbearing age. Various physiological and biochemical properties of this strain were characterized. Both the BIOLOG system and phylogenetic analysis using 16S rRNA sequencing were utilized for identification, and the strain was designated as Lactobacillus plantarum UK-3, and registered in GenBank as [JK266589]. Growth rate, production of organic acids (e.g., lactic acid and acetic acid), and pH during growth were monitored. The maximum concentrations of lactic acid and acetic acid were approximately 684.11 mM and 174.26 mM, respectively, and pH changed from 7.0 to 3.7 after 72 h of incubation. High performance liquid chromatography was used to confirm lactic acid and acetic acid production. Significant antimicrobial activity of the concentrated supernatant was demonstrated against various Gram-positive (e.g., Staphylococcus aureus, Staphylococcus epidermidis, Methicillin-resistant Staphylococcus aureus, Enterococcus faecalis, Neisseria species., Listeria monocytogenes), Gram-negative bacteria (e.g., Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis), and yeast (e.g., Candida albicans) by the plate diffusion method. As a result, the concentrated L. plantarum UK-3 cultures had lower acidity and inhibited the growth of all microorganisms tested, whereas the growth of L. acidophilus was not affected.

서해안에서 분리한 비브리오균의 항생제 감수성 특성

강창호,오수지,소재성,Kang, Chang-Ho,Oh, Soo Ji,So, Jae-Seong 한국미생물학회 2013 미생물학회지 Vol.49 No.2

비브리오균은 해양 및 하구 환경에서 상재하는 균으로 그람 음성 간균, 호염성, 비포자형성의 특징을 가지고 있다. 질병과 관련된 균주로는 Vibrio parahaemolyticus, Vibrio vulnificus, Vibrio cholera 등이 있다. 매년 어패류의 섭취 등을 통해 식중독 사고가 빈번하게 발생하고 있으며, 이에 본 연구에서는 서해안 6곳에서 패류를 채취하여 비브리오균의 분포도를 모니터링하고, 이들의 항생제 내성을 분석하였다. 총 120건의 패류를 TCBS 평판배지와 API 20E kit 기법으로 동정하였을 때 23.3%의 시료에서 비브리오균이 검출되었다. 이들 분리균주들의 항생제 내성을 16종의 항생제에 대해 분석한 결과 82.1%가 각각 vancomycin과 ampicillin에 대해 내성을 보였고, rifampin (71.4%)과 cephalothin (53.6%)에 대한 내성비율도 다른 항생제들에 비해 상대적으로 높은 것으로 나타났다. 분리균주들의 항생제 감수성은 chloramphenicol (92.9%), sulfamethoxazole/trimethoprim (92.9%)과 tetracycline (96.4%)에 대해 가장 높았다. 분리균주들의 약 71.4%가 vancomycin과 ampicillin을 포함하여 3개 이상의 항생제에 대해 다중내성을 보였다. Bacteria of genus Vibrio are Gram-negative, curved, halophilic, nonspore-forming bacteria, autochthonous inhabitans of the marine and estuarine environments. Some of the Vibrio species such as V. parahaemolyticus, V. vulnificus, and V. cholerae are associated with human disease. Each year many people have been suffering from food-borne disease caused by the ingestion of seafood. In this study, we have monitored antibiotic resistance of this microorganism in 6 coastal areas of West Sea by sampling shellfish monthly. Vibrio spp. were detected from 23.3% of 120 samples analyzed using TCBS agar plates as well as API 20E kit. Among 16 antibiotics tested, resistance to vancomycin and ampicillin was observed in 82.1% of the isolates, and Vibrio spp. resistant to rifampin (71.4%) and cephalothin (53.6%) were also high. Most of the isolates were sensitive to chloramphenicol (92.9%), sulfamethoxazole/trimethoprim (92.9%), and tetracycline (96.4%). About 71.4% of the isolates showed multiple drug resistance toward 3 antibiotics including vancomycin and ampicillin.

Trichoderma reesei QM 9414 의 섬유소 분해 효소 생산을 위한 조절변이주의 분리 및 특성에 관한 연구

최건호,구윤모,소재성 ( Kun Ho Choi,Youn Mo Koo,Jae Seong So ) 한국균학회 1998 韓國菌學會誌 Vol.26 No.1

전복(Haliotis discus)에서 분리한 Serratia marcescens가 생산하는 적색 색소의 항균활성

신유진(YuJin Shin),강창호(Chang-Ho Kang),소재성(Jae-Seong So) 한국생물공학회 2016 KSBB Journal Vol.31 No.4

Serratia marcescens characterized by the ability to produce red pigments inhabits various ecological niches. A strain Serratia marcescens PYU was isolated from abalone (Haliotis discus) collected at the West Sea in Korea. The isolated strain was gram-negative, motile, rods like coccus, oxidase-negative, and catalase-positive; and formed red pigment. S. marcescens PYU was grown in the presence of 0~10% (w/v) NaCl, at pH 4~9, and at 10~40°C. The strain PYU produced red pigment, and the extracted pigment showed antibacterial activity against Streptococcus iniae and Lactococcus garviae which has been known as an important fish pathogens. Further studies are underway to elucidate the direct relationship between the red pigment and antibacterial activity.

탄산칼슘 생성 균주의 분리 및 다양한 칼슘원에 따른 토양 고결화

구탁용(Takyong Gu),강창호(Chang-Ho Kang),신유진(Yujin Shin),소재성(Jae-Seong So) 한국생물공학회 2017 KSBB Journal Vol.32 No.3

The physical method used to prevent a landslide has the risk of environmental pollution. Calcite forming bacteria (CFB) have been received increasing attention as a novel and environmental friendly strategy for the soil improvement. In this study, we selected 11 CFB strains with high calcite production. We also examined survivability and calcite productivity of the strains under various stress conditions to select strains with high resistance to various stresses. Two strains was selected by environment stress. Sphingobacterium sp. KJ-32 and Viridibacillus arenosi B-25 precipitate calcite more than other strains at pH 5 and 15oC respectively. Bio-consolidated soil cakes were made using various calcium salts (calcium chloride, calcium acetate, calcium lactate, calcium gluconate) with mixed culture of 2 strains. Among them, the calcite made using calcium chloride was the largest. These observations demonstrate that this bio-consolidation technology has the potential for eco-friendly prevention of landslide and soil improvement.

딱지꽃 (Potentilla chinensis) 추출물의 항염증 효과

강창호(Chang-Ho Kang),한상현(Sang-Hyun Han),소재성(Jae-Seong So) 한국생물공학회 2013 KSBB Journal Vol.28 No.1

In this study, we investigated the anti-inflammation effect of Potentilla chinensis (PC) on Raw264.7 macrophage cells. Ethanol extract of PC decreased the production of nitric oxide (NO) in LPS-stimulated RAW264.7 cells. Ethanol extract was fractioned by n-hexane, chloroform, ethyl acetate, n-butanol, water and each fraction was tested for inhibitory effects on inflammation. Among the sequential solvent fractions, PC chloroform extracts (50, 100, 300, and 500 μg/mL) significantly suppressed LPS-stimulated production of NO. During the entire experimental period, 200 and 300 μg/mL of PC chloroform extracts had no cytotoxicity. LPSinduced NO and prostaglandin E2 (PGE2) production were inhibited by PC chloroform extracts up to 50% and 90% of these productions, respectively. PC chloroform extracts reduced the expression of iNOS and COX-2 gene. These results suggest that PC chloroform extracts exhibit strong effects of anti-inflammation and can be a potential candidate in the treatment of acute and chronic inflammatory diseases.

Lactobacillus crispatus KLB46의 스트레스 전처리시 열 내성 증진효과

곽대영(Dae-Yung Kwak),강창호(Chang-Ho Kang),전한을(HanEul Jeon),소재성(Jae-Seong So) 한국생물공학회 2014 KSBB Journal Vol.29 No.2

Lactobacilli, the dominant species of microorganisms in the vaginal flora of healthy women, play important roles to prevent bacterial vaginosis and other sexually transmitted diseases. In this study, we carried out studies on stress adaptation prior to various stress treatment. We found that heat or salt adapted KLB46 showed higher cell viability than non adapted upon heat stress at 60℃ for 20 min. When chloramphenicol was added during the adaptation process, heat tolerance was abolished. This result suggested that de novo protein synthesis was essential during adaptation.