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벼 組織培養 由來 變異系統의 主要特性 및 種子 蛋白質 電氣泳動相
Seung Yeob Lee(李承燁),Jae Kil Lee(李載吉),Hyun Jung Kang(姜鉉中),Hyun Tak Shin(申鉉卓),Seon Yong Lee(李善龍) 한국육종학회 1995 한국육종학회지 Vol.27 No.3
To select the useful rice variants by tissue culture, anther, isolated microspore or inflorescence of four japonica rice cultivars were cultured in vitro. Regenerated plants showed wider range of variation than original cultivar in several characteristics. 18 variants were selected from the progenies(R₂) derived from anther(AC), isolated microspore(MC) or inflorescence culture(IC). The selected variants(R₂) were investigated for agronomic characteristics and grain quality. Five AC lines derived from Koshihikari were delayed for 4~10 days in heading date than original cultivar. Culm length and panicle length were simultainously reduced in most of the selected lines, while Dongjinbyeo-IC 1 and Norinmochi No. 1-AC 13 were semidwarf without reducing the panicle length. Daecheongbyeo-MC 2, -MC 3, -MC 13 and -MC 21 were more highly tillered than original cultivar. Grain number per panicle of five variants, Dongjinbyeo -AC 7, -IC 1, Daecheongbyeo -MC 3, -MC 13 and -MC 21, was significantly increased and the highest one was 103 grains per panicle in Dongjinbyeo-IC 1. One-thousand-grain weight was significantly reduced in most of the variants except Daecheongbyeo-AC 11 and-AC 13. Milled rice yield of Daecheongbyeo-MC 3, and -MC 21 were significantly increased compared with the original cultivar, but that of other lines was the similar or reduced one. Amylose content and alkari digestibility of selected lines were similar to those of original cultivars. Chalkiness of milled rice were more increased in Dongjinbyeo-IC 1, Koshihikari-AC 3, -AC 4 and -AC 7, and Daecheongbyeo-MC 3 compared with the original cultivars. SDS - PAGE banding patterns of seed protein showed no differance between variants and original cultivars. The specific band(pH 4.2~4.8) of esterase in Norinmochi No. 1-AC 13 was stronger than that in original cultivar, and the band wasn’t detected in non-glutinoues cultivars, Dongjinbyeo and Daecheongbyeo.
Ki Yong Ha(河基庸),Jae Kil Lee(李載吉),Young Doo Kim(金永斗),Seon Yong Lee(李善龍) 한국육종학회 1996 한국육종학회지 Vol.28 No.3
This experiment was conducted to examine the characteristics of brewing rice for the basis of varietal improvement. Among all tested varieties, 1000-grain weight was over 25.4g at nine varieties except five varieties such as Mangeumbyeo, and expressivity and degree of white core in rice grain were respectively over 75.0 and 48.7 percent at eight varieties such as Yangjobyeo. The highly chalky varieties showed low amylose content, low hardness and high water absorption ratio in rice grain. Highly chalky varieties showed higher liquifying power, saccharificating power, baume, total acid and sugar content and rice wine production ratio, but lower alcohol content and residue/wine ratio compared with the other ones.