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동물유래(動物由來) 병원세균(病源細菌)의 각종(各種) 항생물질(抗生物質)에 대한 감수성조사(感受性調査)
김봉환,이재진,김기석,한태우,Kim, Bong Hwan,Rhee, Jae Chin,Kim, Ki Seuk,Han, Tae Woo 대한수의학회 1980 大韓獸醫學會誌 Vol.20 No.2
The antimicrobial drug susceptibility of 439 isolates of animal pathogens recovered from various clinical cases during 1978-79 has been investigated by the use of disk diffusion technique. The majority of 308 strains of Eschericihia coli were highly resistant to bacitracin, erythromycin, penicillin, streptomycin and tetracyclinon while only 0.3 per cent of them were resistant to gentamicin and 3.2 per cent to colistin. The percentages of strains resistant to ampicillin, carbenicillin, cephalothin, chloramphenicol and neomycin were 30.5%, 24.7%, 11:4%, 28.2% and 26.2% and repectively. However, none of E. coli cultures of ovine origin were resistant to ampicillin, carbenicillin, chloramphenicol, colistin, gentamicin, kanamycin, and neomycin. A total of 39 patterns of multipe drug1 resistance of 308 strains E. coli against 9 drugs in general use such as ampicillin, cephalothin, chloramphenicol, colistin, gentamicin, kanamycin, neomycin, streptomycin and tetracycline were observed and the most common multiple resistance patterns were SM, TC pattern (20.5%) and AM, CP, KM, NM, SM, TC pattern (9.7%). None of the 43 cultures of salmonella organism from pigs and chickens were resistant to ampicillin, carbenicillin, cephalothin, colistin, gentamicin and kanamycin; and the majority of the cultures were susceptible to chloramphenicol (90.0%), neomycin (97.7%) and tetracycline (93.0%). All the cultures were found to be resistant to bacitracin and penicillin and the rate of resistant strains to erythromycin and s treptomycin being 79.1% and 41.9% respectively. It was found that the majority of 63 cultures of staphylococcal isolates were resistant to lincomycin, penicillin, streptomycin and tetracycline. The percentages of 63 staphylococcal isolates susceptible to gentamicin, nitrofurantoin, cephalothin, ampicillin, methicillin, bacitracin and chloramphenicol were 98.4%, 98.4%, 95.2%, 93.7%, 93.7%, 92.1% and 92.1% respectively. The 25 cultures of streptococcal isolates were resistant in order of prevalence to streptomycin(88.0%), kanamycin(68.0%), gentamicin (44.0%), tetracycline (44.0%) and methicillin (40.0%) wihle the majority of them were sensitive to ampicillin, bacitracin, chloramphenicol and penicillin.
韓國에서 츄잔病의 發生 및 binary ethylenimine 不活化 츄잔바이러스 豫防藥의 소에서의 免疫原性 試驗
Bong Kyun Park(朴奉均),Jae Chin Rhee(李在鎭),Soo Hwan An(安壽煥),Ho Kyou Moon(文琥奎),Kong Sik Kim(金恭湜),Dong Soo Son(孫東秀),Kwang Won Lee(李光源) 한국예방수의학회 1993 예방수의학회지 Vol.17 No.3
Chuzan disease of which the main clinical abnormalities were impairment of mobility and nervous signs took place in a calf breeding farm in Cheju province during the period of March, 1993. The serological survey indicated that antibody to Chuzan virus was present at a high rate in Cheju province, while it was at a low rate in the Honam area of the mainland. The retrospective sero-epidemiological investigations strongly suggested that Chuzan virus comes to Korea from the Southeast Asian countries and the first outbreak of Korea is simultaneous with that of Japan. A preliminary study for development of an inactivated Chuzan virus vaccine was performed. The binary ethylenimine treated-tissue culture fluid with a gel adjuvant had good immunogenicity in cattle. To control this disease in Korea, an inactivated Chuzan virus vaccine is scheduled for practical use in the field in the near future.
Pig viral diseases causing reproductie failure in Korea
김병한,권창희,안수환,이재진,Kim, Byoung-han,Kweon, Chang-hee,An, Soo-hwan,Rhee, Jae-chin The Korean Society of Veterinary Science 1992 大韓獸醫學會誌 Vol.32 No.3
1988년부터 1990년 6월까지 전국의 양돈장에서 수집된 돼지 유사산 태아 74복에서 바이러스성 원인체 분리 및 혈청학적 진단을 수행하였던 바 다음과 같은 결과를 얻었다. 공시한 74복의 유사산 태아중 44복의 태아 흉강액에서 면역 globulin이 검출되어 전염성 질병감염에 의한 유사산으로 추정되었다. 이중 37%가 바이러스성 유사산으로 나타났으며 유사산의 원인체별 분포를 살펴보면 돼지 파보바이러스가 21%로 가장 높았으며, 뇌심근염 바이러스가 11%, 일본뇌염 바이러스가 9% 등의 순으로 나타났다. 한편 돼지 콜레라바이러스 및 오제스키병 바이러스에 의한 유사산이 각각 1건씩 검출되었으며 동일 유사산 태아에서 2가지 병원체가 중복감염된 예도 관찰되었다.
박종명,황의경,김인천,이재진,정현규,강구환,Park, Jong-myung,Hwang, Eui-kyung,Kim, In-cheon,Rhee, Jae-chin,Chung, Hyun-kyu,Kang, Ku-hwan 대한수의학회 1998 大韓獸醫學會誌 Vol.38 No.1
Piglets' diarrhea is one of the major causes of economic loss in Korean swine industry. To prevent this serious and persistant problem, we have developed systemic farm evaluation and analysis program. The program consists of the checklist and the computerized program. The items of the checklist are selected on the basis of various causative factors related with the piglets' diarrhea including sanitational status, nutritional management, or farm environment. The checklist is composed of 30 items including 6 factors on facility, 8 factors on environment, 6 factors on nutrition and 10 factors on sanitation, respectively. Minimum 1 to maximum 10 points was assigned to each item depending on level or status of farm. The scores calculated by sum of points obtained from each item could find out the problems which the farm was confronted with. To investigate the applicability of a program 150 swine farms raising over 500 heads were selected from 7 provinces throughout the country and surveyed. There were significant correlations between the piglets' diarrhea and the scores of the checklist. Based on above results we revised the checklist which consisted of 22 items including 5 factors on facility, 7 factors on environment, 3 factors on nutrition and 7 factors on sanitation. After that we composed a computerized diagram out of each point of an item which showed and indicated weak factors to be improved promptly. Our present study suggested that this newly established swine farm evaluation program would be a very efficient way to find out the problems and support the systemic control program of the piglets' diarrhea for swine farms.
김재훈,손현주,황의경,황우석,허권,진영화,이병천,이재진,강영배,야마네 이츠로,김대용,Kim, Jae-hoon,Sohn, Hyun-joo,Hwang, Eui-kyung,Hwang, Woo-suk,Hur, Kwon,Jean, Young-hwa,Lee, Byung-chun,Rhee, Jae-chin,Kang, Yung-bai,Yamane, Itsuro,Kim, 대한수의학회 1998 大韓獸醫學會誌 Vol.38 No.1
The Neospora sp. was isolated from the brain of 1 calf via continuous in vitro cultivation in Vero cell. Neospora tachyzoites were observed 45 days after inoculation of the homogenized brain suspension into the Vero cell. The isolated parasite (named tentatively as NCKB-1) was morphologically and ultrastructurally similar to the previously reported Neospora sp isolated in cattle (BPA-1, JPA-1). A comparison of the antigenic reactivity of cultivated tachyzoites with polyclonal antisera to Neospora caninum and Toxoplasma gondii confirmed that this protozoal isolate was similar to N caninum. This is the first report of successful isolation of Neospora sp from cattle in Korea.
김대용,황우석,김재훈,허권,황의경,이병천,진영화,이재진,최상호,Kim, Dae-yong,Hwang, Woo-suk,Kim, Jae-hoon,Hur, Kwon,Hwang, Ui-Gyeong,Lee, Byeong-chun,Jean, Young-hwa,Rhee, Jae-chin,Choi, Sang-ho 대한수의학회 1997 大韓獸醫學會誌 Vol.37 No.3
A case of neosporosis is described in a 6-month-old aborted bovine fetus. Grossly, numerous, well-demarcated, 2-3mm, yellowish white foci were scattered in the heart and skeletal muscle. Histopathologic changes were confined to the brain, heart, and skeletal muscle. Multifocal areas of necrotizing encephalitis were observed in the cerebrum as well as cerebellum. Severe multifocal to coalescing nonsuppurative inflammation was observed in the myocardium and skeletal muscle. The tachyzoites of Neospora caninum were demonstrated in the brain, myocardium and skeletal muscle with immunohistochemical method using Neospora caninum-specific antibody. Based on the histopathology and immunohistochemistry, this case was diagnosed as neosporosis in aborted Korean cattle. This is believed to be the first reported case of bovine neosporosis in Korea.
Isolation of porcine epidemic diarrhea virus (PEDV) in Korea
권창희,권병준,정태성,기영진,허동호,황의경,이재진,안수환,Kweon, Chang-hee,Kwon, Byung-joon,Jung, Tae-sung,Kee, Young-jin,Hur, Dong-ho,Hwang, Eui-kyung,Rhee, Jae-chin,An, Soo-hwan The Korean Society of Veterinary Science 1993 大韓獸醫學會誌 Vol.33 No.2
설사로 폐사한 자돈의 장 절편을 이용하여 돼지 유행성 설사바이러스(PEDV), 돼지 전염성 위장염 바이러스(TGEV), 돼지 로타 바이러스(PRV)에 대한 병인학적 검사를 형광항체반응을 통하여 조사하였던 바 돼지 유행성 설사 바이러스의 감염에 의한 자돈의 폐사를 확인하였다. 간접형광항체검사시 돼지 유행성 설사 바이러스에 대한 양성반응을 보인 가검재료를 이용 Vero세포에 연속 계대한 후 plaque assay를 통하여 크로닝된 돼지 유행성 설사 바이러스 KPEDV-9주를 작성하였다. 돼지 유행성 설사 바이러스에 대한 면역혈청과 바이러스가 분리된 농장에서 채취된 돼지 혈청을 이용 돼지 유행성 설사 바이러스에 대한 구조단백성분을 분석하였던 바 88K(M.W.), 74K, 70K, 58~54K, 54~46K, 44~40K 및 33~32K에 상당하는 단백성분을 검출할 수 있었다. The etiological survey on porcine epidemic diarrhea virus(PEDV) by immunofluorescence antibody test(IFA) showed the positive rusult from the intestines of piglet died from acute diarrhea. The viral agent of PED was also isolated from intestine, which showed positive reaction by immunofluorescence test. After passage in Vero cell, the viral agent was further cloned by plaque purification and designated as KPEDV-9. The immunoblotting analysis using hyperimmune sera and porcine sera revealed the presence of several polypetide bands with molecular weight(M.W.) of 88K, 74K, 70K, 58~54, 54~46K, 44~40K and 33~32K, respectively.
Yun, Hyo-in,Park, Seung-chun,Jiang, Cheng-zhe,Huh, Won,Rhee, Jae-chin,Park, Jong-myung,Choi, Sang-ho,Cho, Joon-hyoung 충남대학교 수의과대학 동물의과학연구소 1994 動物醫科學硏究誌 Vol.2 No.-
세균 감염증으로 인한 돼지 설사증의 효과적인 치료 및 예방을 위해 돼지 설사 분변으로부터 분리한 E coli 와 S typhimurium을 시험균주로 하여 norfloxacin과 국내 가축 치료에 주로 이용되고 있는 여러 항생제를 실험실적 방법으로 병용투여시 두 항생제 간의 상호작용을 checkerboard method를 이용하여 실험하였다. E coli를 시험균주로 실험한 결과, norfloxacin과 colistin의 두 항생제간의 상호작용은 상승작용을 나타내었다. 그러나 norfloxacin과 tetracycline의 병용 처치 실험에서는 길항작용을 보여주었다. S typhimurium을 시험균주로 실험한 결과, norfloxacin과 colistin의 병용 처치 실험에서는 E coli에서와 마찬가지로 상승 작용을 나타내었다. Norfloxacin과 colistin에서의 배합 비율에 따른 E coli에 대한 FIC 값은 norfloxacin이 0.08 그리고 colistin이 0.78일 때 0.38로 가장 항균 작용이 높았으며, S typhimurium에 대한 FIC 값은 E coli에서와 같은 비율에서 가장 활성이 높은 0.25를 보여주어 두 항생제의 항균력은 배합 비율에 따라서 차이가 있음을 알 수 있었다.