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Gut-Specific Delivery of T-Helper 17 Cells Reduces Obesity and Insulin Resistance in Mice
Hong, C.P.,Park, A.,Yang, B.G.,Yun, C.H.,Kwak, M.J.,Lee, G.W.,Kim, J.H.,Jang, M.S.,Lee, E.J.,Jeun, E.J.,You, G.,Kim, K.S.,Choi, Y.,Park, J.H.,Hwang, D.,Im, S.H.,Kim, J.F.,Kim, Y.K.,Seoh, J.Y.,Surh, C. Elsevier North Holland [etc.] 2017 Gastroenterology Vol.152 No.8
<P>BACKGROUND & AIMS: Obesity and metabolic syndrome have been associated with alterations to the intestinal microbiota. However, few studies examined the effects of obesity on the intestinal immune system. We investigated changes in subsets of intestinal CD4(+) T-helper (T-H) cells with obesity and the effects of gut-tropic T(H)17 cells in mice on a high-fat diet (HFD). METHODS: We isolated immune cells from small intestine and adipose tissue of C57BL/6 mice fed a normal chow diet or a HFD for 10 weeks and analyzed the cells by flow cytometry. Mice fed a vitamin A-deficient HFD were compared with mice fed a vitamin A-sufficient HFD. Obese RAG1-deficient mice were given injections of only regulatory T cells or a combination of regulatory T cells and T(H)17 cells (wild type or deficient in integrin beta 7 subunit or interleukin 17 [IL17]). Mice were examined for weight gain, fat mass, fatty liver, glucose tolerance, and insulin resistance. Fecal samples were collected before and after T cell transfer and analyzed for microbiota composition by metagenomic DNA sequencing and quantitative polymerase chain reaction. RESULTS: Mice placed on a HFD became obese, which affected the distribution of small intestinal CD4(+) T-H cells. Intestinal tissues from obese mice had significant reductions in the proportion of T(H)17 cells but increased proportion of T(H)1 cells, compared with intestinal tissues from nonobese mice. Depletion of vitamin A in obese mice further reduced the proportion of T(H)17 cells in small intestine; this reduction correlated with more weight gain and worsening of glucose intolerance and insulin resistance. Adoptive transfer of in vitro-differentiated gut-tropic T(H)17 cells to obese mice reduced these metabolic defects, which required the integrin beta 7 subunit and IL17. Delivery of T(H)17 cells to intestines of mice led to expansion of commensal microbes associated with leanness. CONCLUSIONS: In mice, intestinal T(H)17 cells contribute to development of a microbiota that maintains metabolic homeostasis, via IL17. Gut-homing T(H)17 cells might be used to reduce metabolic disorders in obese individuals.</P>
호마박의 영양적 가치 평가 및 산란계 사료 내 첨가각 사양 성적에 미치는 영향
임호중,안승민,유선종,김용란,안병기,강창원,Im H. J.,Ahn S. M.,You S. J.,Kim Y. R.,Ahn B. K.,Kang C. W. 한국가금학회 2004 韓國家禽學會誌 Vol.31 No.4
본 연구는 호마박의 사료적 가치를 평가하고 산란계의 성적에 미치는 호마박의 첨가 효과를 구명하기 위해 수행하였다. 실험 1에서는 16수의 ISA-Brown 종계 수탉을 공시하여 강제 급여 방법에 의해 호마박의 진정 대사에너지(TME),질소 보정 진정 대사 에너지 (TMEn)및 진정 아미노산 이용률(TAAA)을 조사하였다. 호마박의 TME 및 TMEn은 각각 2.30 kca1/g및 1.99 kcal/g이었고, 15개 아미노산의 평균 이용률은 $76.93\%$로 나타났다. 실험 2에서는 48주령의 ISA-Brown산란계 90수를 3개 처리(대조구, 호마박 $5\%$, 호마박 $10\%$)로 나누어 4주간의 사양 실험을 실시하였다. 실험사료 내 호마박 첨가가 난 생산성 및 계란 품질에 미치는 영향을 조사하였다. 산란계 사료 내에 호마박을 $5\%$및 $10\%$첨가했을 때 난 생산성 및 생산된 계란의 품질에는 큰 영향을 미치지 않은 것으로 나타났다. 호마박 $10\%$ 첨가구에서 난황 내 Cl8:3 $\omega$3의 비율이 가장 높았으나, 통계적인 유의차는 인정되지 않았다. 혈청 내GOT, G-GT, BUN및 cholesterol 조성에 있어서도 처리간에 큰 차이는 발견되지 않았다. 본 연구에서는 호마박을 정확한 원료 평가를 거쳐 사용한다면 산란계 사료에 있어서 $10\%$까지 사용하여도 난 생산성 및 계란 품질을 저하시키지 않았으며, 공시계에게 어떠한 부정적인 영향도 미치는 않았다는 결과가 시사되었다. Two experiments were conducted to evaluate the feeding values of sesame oil meal (SOM) and to investigate the effects of its dietary supplementation on egg production in laying hens. In experiment I, the values of true metabolizable energy (TME), nitrogen corrected true metabolizable energy (TMEn) and true amino acid availability (TAAA) were determined by force-feeding 16 ISA-Brown roosters and collecting the total excreta from the birds, The TME and TMEn of SOM were 2.30 and 1.99 kcal/g, respectively, and the average TAAA of 15 amino acids was $76.93\%$. In experiment 2, a total of ninety, 48 weeks old ISA-Brown layer were randomly divided into 9 groups of 10 birds each and assigned to three experimental diets containing 0, 5 and $10\%$ SOM for 4 weeks (30 birds per treatment). The inclusion of SOM into laying hen diets at the 5 and $10\%$ level did not affect production and quality of egg. The C18:3 $\omega$3 content of egg yolks in the $10\%$ SOM group was higher than the other groups, but not significantly. There were no adverse effects on blood parameters in layers fed treated diets containing $5\%$ or $10\%$ SOM, The results indicate that SOM can be used for layers diet up to $10\%$ without any significant negative effects on egg production and quality.