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유단희 ( Dan Hee Yoo ),이인철 ( In Chul Lee ) 대한미용학회 2021 대한미용학회지 Vol.17 No.4
In the current study, we investigated the biological activities of ethanol extract of unripe Mangifera indica L. var. Irwin using a variety of in vitro and cell culture model systems for investigation of the anti-inflammatory and whitening effects. A total polyphenol, electron donating, 2, 2’-azinobis [3-ethylbenzothiazoline-6- sulfonic acid] (ABTS) radical scavenging assay was used for measurement of the antioxidant activities of unripe Mangifera indica L. var. Irwin. The content of phenolic compounds in unripe Mangifera indica L. var. Irwin was 121.47±0.47 mg/100 g. In measurement of electron donating and ABTS radical scavenging ability of unripe Mangifera indica L. var. Irwin, the effect was shown in a concentration-dependent manner. Inhibition of tyrosinase activity was 40.69% at 1,000 μg/ mL concentration of unripe Mangifera indica L. var. Irwin. An MTT assay was used to evaluate cell viability in order to evaluate the anti-inflammatory and whitening effects of unripe Mangifera indica L. var. Irwin in macrophages (RAW 264.7 cells) and melanoma (B16F10 cells). The result showed more than 95% at a concentration of 500 μg/ mL in RAW 264.7 cells and more than 95% at a concentration of 100 μg/mL in B16F10 cells. Nitric oxide inhibitory activity was confirmed for measurement of anti-inflammatory activity. Consequentially, unripe Mangifera indica L. var. Irwin showed an inhibitory effect of 62.64% at a concentration of 500 μg/mL. The amount of melanin production was measured in order to confirm the whitening effect; the inhibitory effect was 15.16% at a concentration of 100 μg/ mL. Thus, unripe Mangifera indica L. var. Irwin could be applied as an anti-inflammatory and whitening effect and could be used in the future as a safe, natural material for use in cosmetics.
십이지장 궤양 발생에 있어서 H . pylori와 위상피화생의 연관에 대한 연구
박선미(Seon Mee Park),양석균(Suk Kyun Yang),홍원선(Weon Seon Hong),민영일(Young Il Min),이인철(In Chul Lee) 대한소화기학회 1995 대한소화기학회지 Vol.27 No.6
N/A Background/Aims: H. pylori infection and gastric metaplasia are frequently associated in patients with duodenal ulcers. However, the pathogenesis of duwlenal ulcer in relation to H, pilori infecri<in and gastric metaplasia has not reacbed a consistent result, yet. The aim of this study v,as to assess whether H. pylori infection and gastric metapla.ia contrihute to duodenal ulcerogenesis. Methods: Multiple endoscopic biopsy specimens were tnken frorn duodenum and gastric antrum of ?I subjects(21 active duodenal ulcers; 30 scarred duodenal ulcers; 20 normal controls). The specimens were examined histologically for the presence ot' H. pylori, gastric metaplasia, and degree of gastritis and duodenitis. Rapid tissue urease test was performed t'or detection of H, pi krri infc cti<in. Results: The positive rate of H. pylrri in gastric antrurn was not significantly different aniong ttlTCC groups, while it was increasing in duodenum, I,e., normal controls(3%'), scarred DU's(37,r), active DU's(62%) in ascending order. The incidence of duodenal gastric metaplasia in the active DU'.I was 95.2% in contrast with the scarred duodenal ulcer's(72.07<) and the nonnal contrnls(2i.04) Diffuse gastric metaplasia lesions were more prevalent in active duodenal ulcers. 1n the duodenum, a strong association was shown between the H. pylrri and gastric rnetaplasia. The severity ot' duodenitis were significantly increased in the order ot' groups of normal controls, scarrecl duodena1 ulcers, and active duodenal ulcers. In duoclenum, there was a strong association with the grade c>f' intlammation anci H. pylori. Conclusions: These results suggest that gaslric metaplasia in the duodenum and concurrent H. pilori infection may play a synergistic role in the pathogene.I. Vt' duodenal ulcer. (Korean J Gastroenterol 199S;27: 617-62S)
애플망고 가지 추출물의 항산화 활성 및 대식세포내의 iNOS, COX-2의 염증인자 발현 저해 효과
유단희 ( Dan Hee Yoo ),이인철 ( In Chul Lee ) 대한미용학회(구 대한미용과학회) 2020 대한미용학회지 Vol.16 No.4
In the present study, anti-oxidant and anti-inflammation activities of distilled water extract (AMBW) and 70% ethanol extract (AMBE) of Mangifera indica L. var. irwin branches were investigated. The total polyphenol content of M. indica L. var. irwin branch extracts was 523.24±1.03 (AMBW), 556.15±2.80 (AMBE) mg TAE/100 g dry weight. The anti-oxidant activity of the extracts was estimated using electron donation and scavenging activity of 2,2'-azino bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radicals which increased with concentration. To measure the anti-inflammation effects, RAW 264.7 cells treated with lipopolysaccharide were used. The cell viability of M. indica L. var. irwin branches extracts in RAW 264.7 cells was measured with 3-[4,5-dimethyl-thiazol-2-yl]-2,5- diphenyl-tetrazoliumbromide (MTT) assay. The results of MTT assay showed more than 95% refers to cell viability at the 100 μg/mL of AMBW and AMBE. The production of nitric oxide (NO) analyzed by griess assay decreased with increasing concentration of the extracts. The mRNA expression toward inducible NO synthase and cyclooxygenase-2 was confirmed using reverse transcription-polymerase chain reaction and inhibited the treated M. indica L. var. irwin branches extracts. In conclusion, M. indica L. var. irwin branch extracts were confirmed to have antioxidant and anti-inflammation properties and can be used as a natural ingredient of cosmetic materials.
몰로키아(Corchorus olitirius L.) 추출물의 생리활성에 대한 연구
유단희(Dan-Hee Yoo),이인철(In-Chul Lee) 한국인체미용예술학회 2021 한국인체미용예술학회지 Vol.22 No.2
In this study, the effects of the physiological activity of the Corchorus olitirius L. were verified and the applicability as a cosmetic material was reviewed. The total polyphenol content was expressed as 396.44±0.42 mg/100g dry in the water extract of Corchorus olitirius L. (COWE) and the content was expressed as 458.13±0.42 mg/100g dry in the 70% ethanol extract of Corchorus olitirius L. (COEE). The antioxidant effects were measured through an experiment evaluating the electron-donating ability and ABTS radical scavenging activities. In order to verify the whitening effects, tyrosinase inhibitory activity was measured and results showed an effect of 11.82% (COWE) and 48.21% (COEE) at 1,000 μg/mL, respectively. An MTT assay was conducted in RAW 264.7 and B16F10 cells to assess the cell survival rate; it was found that no toxicity was apparent at 100 μg/mL. Active suppression of nitric oxide (NO), an inflammation inducer, was measured to verify the anti-inflammatory efficacy. In the NO assay, experiments were conducted using a concentration of 100 μg/mL which led to a 95% survival rate. The NO assay was measured using Griess reagent and compared to the lipopolysaccharide treatment group and non-treatment group. The inhibition of cellular melanin synthesis was measured to determine the whitening effects. At 100 μg/mL, the inhibition activities of COWE and COEE were 15.69% and 22.67%, respectively. It has been confirmed that COWE and COEE are effective antioxidants, have whitening effects, and are anti-inflammatory agents. In particular, the use of COWE and COEE as natural materials for cosmetics was confirmed to have excellent effects.
이인철(In-Chul Lee),김미경(Mee-Kyung Kim) 한국인체미용예술학회 2015 한국인체미용예술학회지 Vol.16 No.4
The stability of Onchungeum ethanol extract was investigated to determine its applicability as a functional cosmetic material. For its industrial application, the cosmetics were prepared with a water/oil/water(W/O/W) multiple emulsion. The results of the stability test showed that the W/O/W multiple emulsion containing ethanol extract of Onchungeum was highly stable for 60 days without change in particle stability, pH, viscosity, temperature and exposure to artificial lighting. These results suggest that an ethanol extract of Onchungeum can be a useful functional cosmetics material for improving skin diseases.