Brassica屬 植物에 있어서 自家不和合性 관련 遺傅子 SRA의 遺傳性

盧一燮(Nou Ill Sup),李孝淵(Lee Hyo Yeon),李善河(Lee Seon Ha) 한국원예학회 1994 Horticulture, Environment, and Biotechnology Vol.35 No.5

Brassica campestris로부터 自家不和合性 관련 SLG⁴⁸ cDNA의 cloning 및 sequencing

Ill Sup Nou(盧一燮),Hak Soon Choi(崔鶴淳),Hyo Yeon Lee(李孝淵) 한국육종학회 1996 한국육종학회지 Vol.28 No.1

A cDNA sequence homologous to the Brassica self-incompatibility locus specific glycoprotein sequence was isolated from stigmas of B. campestris plants homozygous for the SLG⁴⁸-allele. The relative molecular mass and isoelectric focusing value were calculated to be 50~60KD and pI 9.5, respectively. SLG⁴⁸ cDNA library consisting of 2.5×10⁵ recombinant clones was obtained from the poly (A)⁺ RNA. The cDNA were ligated to λZAPII arms. The selected cDNA clone was subcloned by in vivo excision using the helper phage R408 and E. coli (JM109). The resultant recombinant plasmid was sequenced with Sequenase Ver 2.0 and a deletion kit. The cDNA contains on open reading frame of 1137bases (379 amino acids), a non-coding 3’ region and a poly (A) tail, six N-glycosylation site. Overall the SLG⁴⁸ and SLG⁴⁵ or SRA⁰¹ sequences showed 80.4%, or 53.3% homology at the amino acid level. The SLG⁴⁸ sequence includes 12 cysteine residues in the carboxyl half of the molecule which are conserved with respect of the SLG⁴⁵ and SRA⁰¹. It is indicates that this common characterization of cysteine residues does not specify the self-incompatibility response.

배추 및 Chromobacterium 유래 병저항성 관련 유전자의 분지적 특성

노일섭(Ill-Sup Nou),이인호(In-Ho Lee),바타아르빈톨가(Arvintulga Bataa) 한국생물환경조절학회 2007 한국생물환경조절학회 학술발표논문집 Vol.16 No.2

자가부화합성 (自家不和合性) Brassica campestris 에 있어서 단일유전자좌가설에 (單一遺傳子座假設) 의해 분리되지 않는 S - 유전자 계통의 분석

노일섭(Ill Sup Nou),이효연(Hyo Yeon Lee),김진호(Jin Ho Kim) 한국식물학회 1993 Journal of Plant Biology Vol.36 No.2

Self-incompatibility in Brassica campestris is controlled by multi-allele system in a single genetic locus, the S locus, and it is elucidated that S-glycoproteins are S gene products. In this experiments, we exaimined the genetic mode(pollen tube behavior and segregation of S-glycoprotein), characteristic of S-glycoproteins and DNA constitution within unclear genome of S gene family that unexplained by single locus model, and investigated the segregation pattern of S-glycoproteins in bred F_1 generation. By diallel cross among the 15 plants within one family the existence of three types of homezygotes and three types of heterozygotes were observed, and segregation of S-allele could not explained by single locus model. From the results of IEF-immunoblot analysis for non-segregated individual plant, the segregation pattern of S specific bands was corresponded with results of diallel cross except with one case(S^αS^α genotype). The molecular weight of 6 different S-genotype varied in near by 50 kD, and each genotype expressed with 2 or 3 bands. Specific bands in S^αS^α, S^bS^b, S^cS^c has almost similar molecular weight between them. Southern analysis of genomic DNA probed with S-glycoprotein cDNA for 6 different genotypes revealed that there are clear difference in polymorphism, multiple bands of hybridization, when restriction enzymes of EcoRI were used. It could be assumed that there are several sequences related to the S-glycoprotein structural genes within their nuclear genome. Therefore, we suggested the possibilities that S-allele system could be controlled by multi-locus, that dominance-recessive interactions could be explaned by modifier gene or supressor gene based on the results of abnormal segregation of S-glycoprotein in bred F_1. The F_2 analyses are progressing in now.

韓國 自生茶 集團과 日本의 綠茶品種 Meiryoku 간의 化學成分 比較

盧一燮(Ill Sup Nou),山口優一(Yuichi Yamaguchi),李善河(Seon Ha Lee) 한국차학회 1997 한국차학회지 Vol.3 No.1

For using as a selection indicator and an estimation data of adaption of teas in breeding system, major chemical components among spontaneous populations of Korean tea plant and "Meiryoku" of an excellent Japanese cultivar were analyzed and compared. The average contents of theanine, the major amino acid which improves its taste in tea, were ranked in sequence of Seonamsa (1.220%) > Beomwangri (1.085%) > Meiryoku (0.943%) > Baekyangsa (0.335%) > Songgwangsa (0.314%) > Dasolsa (0.238%). Arginine contents were in the range from 0.013 to 0.226%. Significantly, Arginine contents of Seonamsa population were extremely higher(about 21 times) than those of "Meiryoku". Total flavanol contents were ranked in sequence of Meiryoku (23.58 mg/g) > Baekyangsa (22.06 mg/g) > Beomwangri (19.72 mg/g) > Seonamsa (16.90 mg/g) > Dasolsa (15.34 mg/g) > Songgwangsa (15.13 mg/g). The lower grade flavanol contents of Korean populations may be possible to use as a genetic resources for green tea cultivar. Total polysaccharide contents, highly varied depending on developmental stage of leaf and manufacturing process, were ranked in sequence of Seonamsa (1.56%) > Dasolsa (1.50%) > Songgwangsa (1.45%) >Meiryoku (1.34%) > Baekyangsa (1.29%) > Beomwangri (0.81%). Contents of ascobic acid and tocopherol in Beomwangri and Dasolsa were higher than those of other populations or cultivar.

PCR-핵산지문법에 의한 국내 재래종 쪽파의 유전적 다양성분석

노일섭(Ill-Sup Nou),양승렬(Seung-Yul Yang),우서(Seo Woo) 한국원예학회 2010 한국원예학회 학술발표요지 Vol.2010 No.5

同形化型 自家不和合性에 관한 硏究動向 Ⅱ. 配偶體型 自家不和合性에 관한 遺傳分析 및 分子的 硏究

Ill Sup Nou(盧一燮),Hyo Yeon Lee(李孝淵),Jin Ho Kim(金晋鎬) 한국육종학회 1993 한국육종학회지 Vol.25 No.1

In gametophytic self-incompatibility, pollen tubes expressing S-alleles identical to those expressed in the style cease growth in the upper third of the style. The S-allele products of pollen and discriminate between self and cross-pollen. S-allele-associated pistil proteins(S-glycoproteins) have been identified in many Solanaceae. The sequences of a number of Solanaceous S-glycoprotein have been deduced from the corressponding cDNA sequences and compared their primary structure between them. The biological function of S-glycoproteins in the self-incompatibility interactions has not been demonstrated. S-glycoproteins corresponding to the most S-alleles isolated from style extracts of Nicotiana, Petunia, Solanum has a ribonuclease(RNase) activity. But the arrest of pollen tube growth was not simply explained by RNase action, and transcription and glycosylation process is necessary for the expression of self-incompatibility, plural genes or action sites may be involved in an S-gene. There are some unresolved question in RNase hypothesis ; How can be explain S-allele specificity? What is substrate of RNase? What is self-incompatibility related substance in pollen side against style side? Anyhow, at the moment, differences between gametophytic and sporophytic systems are clear, and we are inevitably believe that plants having different structure of flower have obtained respective systems to secure the genetic heteroveneity in the course of evolution.

同形化型 自家不和合性에 관한 硏究動向 Ⅰ. 胞子體型 自家不和合性에 관한 遺傳分析 및 分子的 硏究

Ill Sup Nou(盧一燮),Hyo Yeon Lee(李孝淵),Jin Ho Kim(金晋鎬) 한국육종학회 1993 한국육종학회지 Vol.25 No.1

In self-incompatibility, the rejection if self pollen occurs at the initial site of pollination, the stigma, where pollen germination either does not commence or is aborted before invasion of the papillar cell wall by the pollen tube. Recently, analysis of self-incompatibility in Brassica has focused on the study of a class of stigma glycoproteins, the S-locus glycoprotein(SLG). The level of SLG corresponds with the onset of self-incompatibility and the polymorphisms of these molecules correlate with S-allele polymorphism and genetic segregation of S-allele. In these decades, knowledge have been accumulated explosively on S-alleles at DNA and protein levels. An evidence that the function of SLG was suggested by a database survey in respective systems tells us recent trends of studies in this area. These studies revealed the difference of the structure and function of S-alleles in sporophytic and gametophytic systems. Siganl transduction system as found in vertebrates seems to be involved in sporophytic incompatibility, since SRK(S-receptor kinase) genes were found. SRK gene was shown to encode a putative receptor protein kinase with a cytoplasmic domain exhibiting homology to serine/threonine protein kinase and an SLG-homologous extracelluar domain was speculated to be involved in ligand binding. Despite of the explosive increase of data, many important question are still unsolved What is the pollen specific substance that participate in the recognition reaction? Can variation of S-alleles be explained only by the structural difference of SLG? How is the whole structure of S-genes? These situation give us an impression that we are still very poverty in our knowledege of self-incompatibility, but the rush of papers in a couple of years gives us also a hope that the mechanism of self-incompatibility will be recongnized by us on a sound basis in very near future.

ICT 원격제어 system 이용 식물진단, Phenomics 연구현황 및 전망

정유진,노일섭,김용권,김회택,강권규,Jung, Yu Jin,Nou, Ill Sup,Kim, Yong Kwon,Kim, Hoy Taek,Kang, Kwon Kyoo 한국식물생명공학회 2016 식물생명공학회지 Vol.43 No.1

Remote sensing는 각종 센서를 이용하여 지표면, 물, 요소 기술에 대해 비접촉, 비파괴적인 방법으로 필요한 정보를 얻어내는 기술이다. 이들 기술은 센서 등의 요소 기술 뿐만 아니라, 센서를 탑재하는 플랫폼과 정보 통신 기술(ICT) 등을 복합적으로 이용한다. 특히 농업 분야에서는 ICT를 중개로 기상이나 토양 등의 환경 정보와 작물 정보를 측정하여 수치화하고 클라우드 컴퓨팅에 의해 생산 단계뿐만 아니라 유통 및 소비 단계까지 관리하는 스마트 농업에 크게 기여한다. 식물을 측정하기 위해서는 비파괴 비접촉 bioimaging (remote imaging)을 포함한 식물기능 remote sensing 기술개발이 필요하다. 또한 식물 과학 분야에서도 유전자 세포 수준에서 개체 수준까지를 대상으로 한 bioimaging 연구가 활발히 진행되고 있다. 최근 들어 표현형 연구를 통해 환경과 유전자형의 관계를 구명하는 phenomics 연구가 활발히 진행되고 있다. 따라서 본 논문에서는 식물기능 원격탐사의 기술동향, 식물진단 및 식물환경응답해석과 식물 phenomics 연구현황에 대해 고찰하였다. Remote Sensing (RS) is a technique to obtain necessary information in a non-contact and non-destructive method by using various sensors on the surface, water or atmospheric phenomena. These techniques combine elements such as sensors, and platform and information communication technology (ICT) for mounting the sensor. ICT has contributed significantly to the success of smart agriculture through quantification and measurement of environmental factors and information such as weather, crop and soil management to distribution and consumption stage, as well as the production stage by the cloud computer. Remote sensing techniques, including non-destructive non-contact bioimaging (remote imaging) is required to measure the plant function. In addition, bioimaging study in plant science is performed at the gene, cellular and individual plant level. Recently, bioimaging technology is considered the latest phenomics that identifies the relationship between the genotype and environment for distinguishing phenotypes. In this review, trends in remote sensing in plants, plants diagnostics and response to environment and status of plants phonemics research were presented.

국내 멜론 흰가루병균의 race 동정 및 시판품종의 흰가루병 저항성 판별

김회택,박종인,노일섭,Kim, Hoy-taek,Park, Jong-in,Nou, Ill-sup 한국식물생명공학회 2016 식물생명공학회지 Vol.43 No.1

국내 멜론 및 박과채소 재배농가(안성, 이천, 영암, 창녕, 순천)에서 발병하고 있는 멜론 흰가루병균을 채집한 후 흰가루병균의 형태학적 특성을 이용한 종류 구분 및 멜론 race 판별계통을 이용한 국내에서 발병하고 있는 흰가루병균의 race를 확인하였다. 그 결과 국내 멜론 흰가루병균의 종류는 Podosphaera xanthii (syn. Sphaerotheca fuliginea)였으며, 기존에 보고된 race 1, N2, A와 판별결과가 일치하는 3 종류의 균주가 분리되었다. 또한 기존에 보고된 race (1, N1, N2, A, S, 5)와 검정 결과가 다른 새로운 race의 흰가루병균 2종이 동정되었다. 그리고 5개의 분리 및 동정된 흰가루병균을 이용하여 국내 시판중인 멜론 15품종에 대한 저항성을 조사한 결과, race1에 대해서는 15품종 중 9품종이 저항성을 보였다. Race N2와 A대해서는 소수의 품종에서 저항성을 보였으며, 새로운 race BN968에서는 15품종 모두가 이병성을 나타내었다. 또한 멜론 흰가루병 race 1 저항성 DNA 마커 KPMR1M-1과 KPMR1M-2에 의한 이병성 및 저항성 판별 결과와 race 1 접종 결과는 일치하였다. 따라서 국내 멜론 품종 육종에 있어서 다양한 race 특이적 마커 개발 및 이를 활용한 복합 race 저항성 품종 개발이 요구된다. Powdery mildew is an important disease of the melon (Cucumis melo L.). Seven isolates of powdery mildew fungi were collected from five locations in Korea; Anseong (DH487), Icheon (BN103, BN625, BN968), Yeongam (YA141), Changnyeong (CN582), and Suncheon (SN102). All 7 fungi had a similar trend of conidial chain and conidiophore development as Podosphaera xanthii with fibrosin bodies in mature conidia. Among them, 2 isolates of powdery mildew fungi; CN582 and SN102 showed similar responses to resistance against powdery mildew as the previously reported race 1 and race N2. The isolates YA141 and BN103 showed similar responses as like as race A. However, three isolates of powdery mildew fungi (BN625, BN968, and DH487) showed different responses compared to the previously reported races (1, N1, N2, A, S, and 5). Therefore, these three isolates could be designated as new races in melon. Nine out of 15 commercial melon cultivars in Korea showed resistance to race 1 (CN582). However, the new race BN968 invaded all 15 cultivars. Results of the two molecular markers were consistent in response to disease development by race 1 of Podosphaera xanthii in case of the above mentioned cultivars. This study confirmed the presence of new melon powdery mildew fungi in Korea which are similarly notorious as like as the previously reported race 1. Therefore, breeders can use these two molecular markers for breeding melon in Korea that is resistant to race 1 and as well as to some other races.