흡연자와 비흡연자의 치은섬유아세포에서 니코틴과 NNK가 부착과 성장에 미치는 영향

김일영,박미영,최성호,조규성,김종관,채중규,Kim, Il-Young,Park, Mi-Young,Choi, Seong-Ho,Cho, Kyoo-Sung,Kim, Chong-Kwan,Chai, Jung-Kiu 대한치주과학회 1998 Journal of Periodontal & Implant Science Vol.28 No.4

In order to study the effects of cigarette smoking on periodontal tissue, gingival fibroblast from the smoking and nonsmoking groups were cultured and each group were treated with nicotine(50ng/ml,100ng/ml) and NNK(50ng/ml, 100ng/ml) to test their attachment ability at time intervals of 30minutes, 60minutes, 90minutes, 120minutes, and 240minutes. Using the same method, the growth each group treated with nicotine and NNK in order to compare their attachment ability and growth rate was done. The Results are as follows. 1. In comparing the attachment ability and growth rate between the smoking and non-smoking group were significantly higher in all time intervals. 2. When the attachment ability was com-pared among these two groups after treatment with nicotine and NNK, the non-smoking group showed decrease in attachment ability while the smoking group was not affected. 3. The growth rate of these two groups were compared after treating with nicotine and NNK. The growth rate of fibroblast from the non-smoking group decreased while fibroblast from the smoking group was not affected. These results suggest that fibroblast from the non-smoking group showed higher attachment ability, growth rate, and sensitivity to nicotine and NNK. This implies that fibroblast from the non-smoking group is a more reliable source in testing the cytotoxicity of nicotine and NNK. Also it could be reasonable to think that nicotine and NNK is a probable cause for problems in attachment and repair mechanism.

Renal Sympathetic Denervation Induces Acute Myocardial Inflammation through Activation of Caspase-1 and Interleukin-1β

Dong Won Lee(이동원),Il Young Kim(김일영),Ihm Soo Kwak(곽임수) 한국생명과학회 2018 생명과학회지 Vol.28 No.2

원심성, 구심성 교감신경 신호는 고혈압 및 심부전의 발생과 밀접한 관련이 있다. 혈관 내 카테터를 이용한 교감신경절제술(RDM)은 난치성 고혈압의 대체치료로 시행되어 왔다. 시술과 관련하여 장기간 신장의 안정성에 대해서는 보고가 있었으나 단기간 심근의 안정성에 대한 연구 결과는 없었다. 저자들은 RDN 시술로 인한 교감신경차단 후 염증성 심근손상이 발생할 수 있음을 가정하여 실험으로 검증하고자 하였다. 25마리의 암컷돼지를 3군으로 나누고–정상대조군(n=5), Sham군(n=5), RDN 시술군(n=15)–RDN 시술군을 시술 후 sacrifice 시기에 따라 다시 세분하였다–시술 후 즉시 sacrifice한 RDN-0 (n=5), 시술 1주 후 sacrifice한 RDN-1 (n=5), 시술 2주 후 sacrifice한 RDN-2 (n=5). 조영제의 영향을 배제하기 위해 설정했던 Sham군과 정상대조군 간에는 의미있는 차이를 보이지 않았다. IL-1β, TNF-α 등의 염증 싸이토카인은 시술 1주 후 증가하여 2주째 감소하였다. 항염증 싸이토카인 IL-10은 시술 직후부터 증가하여 2주째 감소하였다. Inflammasome에 의해 위험신호(danger signal)를 전달받고 활성화되는 Caspase-1 및 inflammasome을 매개하는 도메인 ASC는 시술 직후 활성화되어 발현이 증가하였고 2주까지 지속되었다. 그러나 caspase-1을 매개할 것으로 추정되었던 NLRP3 inflammasome의 발현은 의미있는 차이를 보이지 않았다. RDN 시술에 의한 교감신경차단은 caspase-1, IL-1β 등의 활성화에 의해 염증성 심근손상을 초래할 수 있으며 RDN 시술 후에는 그 위험성을 고려해야 하겠다. NLRP3 외에 다른 NLRP inflammasome pathway의 관련성에 대한 추가연구가 필요하다. Efferent and afferent sympathetic nerves are closely related to the development of hypertension and heart failure. Catheter-based renal sympathetic denervation (RDN) is implemented as a strategy to treat resistant hypertension. We investigated whether RDN procedure causes inflammatory damage on myocardium in the early phase of sympathetic denervation. Twenty-five female swine were divided into 3 groups: normal control (Normal, n=5), sham-operated control (Sham, n=5), and RDN groups (RDN, n=15). The RDN group was further subdivided into 3 subgroups according to the time of sacrifice: immediately (RDN-0, n=5), 1 week (RDN-1, n=5), and 2 weeks (RDN-2, n=5) after RDN. There were no significant changes in the clinical parameters between the normal control and sham-operated group using contrast-media. In the myocardium, inflammatory cytokines, IL-1β and TNF-α increased at the first week, and then decreased at the second week after RDN. Anti-inflammatory cytokine, IL-10 increased immediately, and then decreased at the second week after RDN. Caspase-1 activity and apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) expression increased immediately after RDN until the second week. However, nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing protein 3 (NLRP3) expression did not show any significant differences among the groups. The RDN can cause acute myocardial inflammation through activation of caspase-1 and IL-1β. We should pay attention to protecting against early inflammatory myocardial damage after RDN.

부가적인 전기 장비품의 인증 방안

김일영,서장원,Kim, IL-Young,Suh, Jang-Won 항공우주시스템공학회 2007 항공우주시스템공학회지 Vol.1 No.4

The interest about the installation and certification for miscellaneous non-required electrical equipment is being increased in the domestic aviation industries. In this paper, the minimum applicable requirements in Airworthiness Standard and the acceptable means of compliance which can be applied to installation and certification for miscellaneous non-required electrical equipment is being researched. As a case study, galley certification procedures are presented by electrical load analysis, installation of warning devices and installation requirements.

경영자로서 한의사 역량에 대한 교육요구도 분석

김일영,임병묵,김동수,Kim, Il-young,Lim, Byungmook,Kim, Dongsu 대한예방한의학회 2015 대한예방한의학회지 Vol.19 No.2

Objective : This study aims to investigate the priority of education needs of managerial competencies for Korean Medicine doctors. Method : Self-administered questionnaires were sent via on-line survey system to 4,107 Korean Medicine (KM) doctors who were registered in the Association of Korean Medicine and whose business places were in Seoul. The cognition of necessities, present levels, and future importance of managerial competencies were measured. To measure the degree of educational needs on health management, t-test and Borich method were used. Results : The response rate was 1.9%(79 persons) and 68 responses were included in the analysis. The cost-effectiveness studying ability and the public relations ability were the most important competencies. Analyzing the competency groups by t-test and Borich method, the marketing group was the most important competency group. Many KM doctors (53 persons; 68.9%) who answered this questionnaire expressed needs of the business administration course. Conclusion : KM doctors conceded the necessity of business competency to manage their hospitals or clinics. Academic institutions needs to consider the implementation of business administration courses for KM students in accordance with needs of the managerial competencies.

하수오(何首烏)가 methotrexate로 유도된 흰쥐의 면역기능저하(免疫機能低下)에 미치는 영향(影響)

김일영,이상재,김광호,Kim Il-Young,Lee Sang-Jae,Kim Kwang-Ho 대한예방한의학회 2000 대한예방한의학회지 Vol.4 No.2

To examine the effects of Polygonum multiflorum on white rats with deteriorated immunity caused by methotrexate, first of all, methotrexate was fed to the rats once a day for 4 days. After the immune response of the rats are dereriorated, dried extracts of Polygonum multiflorum mixed in water was fed to the white rats once a day for 144days. The next conclusion was made by examining the rates of B-cells and T-cells of the peripheral blood and the changes in rates of CD4+ T-cells and CD8+ T-cell of the blood sampled from the spleen and peripheral region . Polygonum multiflorum has an effect of increasing immune responses on white rats with deteriorated immunity caused by MTX. Especially the count of CD4+ T-cells of the peripheral blood and the count of CD4+ T-cell of the spleen proved the significant effect o( increasing immune responses statistically . Verification of the effects of the Polygonum multiflorum should be made through comparitive .studies using various immune response indexes. Also additional studies for a modern and practicible application of Polygonum multiflorum seems to be needed.

전치부 Diastema Closure

김일영,Kim, Il-Young 대한심미치과학회 2002 Journal of the Korean Academy of Esthetic Dentistr Vol.11 No.2

균체 지질 생산에 관한 연구

김일영,정동효,Kim, Il -Young,Chung, Dong-Hyo 한국식품과학회 1985 한국식품과학회지 Vol.17 No.2

A potential lipid producing strain of Penicillium sp. was isolated from natural source. Penicillium sp. was cultivated in an appropriate medium containing 6% of glucose as a corbon source, ammonium nitrate as a nitrogen source, C:N ratio 200, pH 4.0 for a period of 17 days at $35^{\circ}C$. Under the condition, the lipid content was 64.2% of dry cell weight. The total lipid produced was 13.7g/100g of glucose consumed. The proportion of nonpolar and polar lipid fractions was 92.2% and 7.8%, respectively. The nonpolar lipid compositions of lipid produced under optimum condition were 5.3% of free fatty acids, 6.8% of free sterols, 9.3% of partial glycerides and 72.0% of triglycerides. The major fatty acids of total lipid were 20.1% of palmitic acid, 21.6% of linoleic acid and 53.3% of oleic acid. 자연계로 부터 지질생산 균주, Penicillium sp.을 분리하였다. 이 균의 최적 지질생산 조건은 탄소원으로 포도당 6%, 질소원으로 ammonium nitrate, 배양기간 17일, C:N ratio 200, 배양 초기pH 4.0, 진탕배양(100 rpm), $35^{\circ}C$이었고 이때 생산된 균체량은 2.26g/200ml, 지질함량은 64.2%. 지질생성률 13.7g이었다. 최적 지질생산 조건에서 생산된 지질 중에 비극성 지질의 함량은 92.2%, 극성 지질은 7.8%이었다. 비극성 지질의 조성은 free fatty acid, free sterol, partical glyceride, triglyceride이며 이들중 triglyceried가 72.0%로 대부분을 차지하고 있다. 총지질의 주요 지방산은 oleic acid 53.3%, linoleic acid 21.6%, plamitic acid 20.1%로 oleic acid가 가장 많이 존재하며 이들 주요 지방산이 전체 지방산의 90%이상을 차지하고 있다. 식물성 유지인 땅콩 olive oil의 지방산 조성과 비슷한 경향을 나타내고 있다.

박정희체제 18년 : 발전과정에 대한 분석과 평가

김일영(Kim Il-Young) 한국정치학회 1995 한국정치학회보 Vol.29 No.2

본 논문의 목적은 박정희체제 18년을 한국 산업화과정의 단계와 관련시켜 자리매김해 보는 것이다. 후발산업화의 초기 단계였던 이 시기를 제대로 평가하기 위해 본논문에서는 먼저 비교사적 관점에서 영국과 프러시아로 대변되는 선발 및 후발산업화 경험을 재검토했다. 그 결과 적어도 경험적으로는 산업화 초기 단계에서 발전지향형 권위주의체제와 자본주의적 경제발전 사이에 ‘선택적 친화력’이 있다는 이론적 명제를 도출했다. 그리고 이런 초기 산업화과정에서 초래된 수많은 부작용은 박정희체제 탓만도 아니고 산업화의 불가피한 부산물만도 아닌 양자가 책임을 나누어 가져야 할 문제라고 평가했는데, 그 과정에서 인간의 행위나 역사에서 나타나는 결과의 패러독스를 살펴보기 위해 베버의 행위의 이념형적 분류-책임윤리와 신념윤리-를 원용했다. 아울러 기존의 리더십론이나 편승론과는 다른 세계체제의 헤게모니적 구조, 사회계급, 그리고 국가의 성격 등의 요인을 포함한 과학적 분석틀을 구성하여 이 시기에 이루어진 경제발전과정에 대한 분석을 시도하였다. 끝으로 1980년을 계기로 한국사회에서 발전지향형 권위주의체제는 유효성을 상실했기 때문에 그 후의 전두환체제는 시효가 만료된 모델을 인위적으로 연장시킨 것으로 자리매김할 수 있고, 근래 재평가의 명분 아래 박정희를 다시 들먹이는 것은 망령(亡靈)을 불러내는 망령(妄靈)이라는 것이 본논문의 결론이다.

국내 우주용 제품에 적용되는 공정에 대한 고찰

김일영(Il-Young Kim) 항공우주시스템공학회 2017 항공우주시스템공학회 학술대회 발표집 Vol.2017 No.4

마르크스주의 정당논의

김일영(Il Young Kim) 한양대학교 아태지역연구센터 1990 중소연구 Vol.14 No.2