Molecular Characterization of cDNA Encoding the ADP-Ribosylation Factor-like Protein (ARL), BcARF3, from Chinese Cabbage Flower Bud

Hong, Joon Ki,Lee, Eun Young,Jin, Zheng Lu,Kim, Ho Il,Hong, Jong Chan,Choi, Young Ju,Yun, Dae-Jin,Lee, Sang Yeol,Bahk, Jeong Dong,Cho, Moo Je,Lim, Chae Oh 한국유전학회 2002 Genes & Genomics Vol.24 No.4

A cDNA encoding a small GTP-binding protein highly similar to Arabidopsis ADP-ribosylation factor 3 (ARF3) was isolated from the Chinese cabbage flower bud cDNA library. The cDNA clone contained an open reading frame encoding a 182-amino acid polypeptide with a calculated molecular mass of 20.3 kDa. A multialignment and phylogenetic analysis demonstrated that all the plant genes evaluated were clustered in a major evolutionary tree branch, indicating the need for a new plant ADP-ribosylation factor (ARF) and ARF-like protein (ARL) subfamily classification. Several ARF or ARL genes were identified in Chinese cabbage genomic DNA by Southern analysis. ARL transcript levels, differentially expressed in different organs of the Chinese cabbage, were particularly high in the leaves. Accumulation of ARL transcript was nearly constant in seedlings treated with growth regulators. However, the ARL transcript was preferentially expressed in suspension culture cells following salicylic acid (SA) treatment but not following treatment with hydrogen peroxide (H_20_2), jasmonic acid (JA), or nitric oxide (NO). Thus, a SA signaling pathway may regulate ARL expression in Chinese cabbage suspension culture cells.

Analysis of microstructure produced by fast tool servo machining

Hong Lu(여굉),D. W. Lee(이득우),S. J. Lee(이승준) 한국정밀공학회 2013 한국정밀공학회 학술발표대회 논문집 Vol.2013 No.10월

A new high-order response surface method for structural reliability analysis

Hong-Shuang Li,Zhen-Zhou Lu,Hong-Wei Qiao 국제구조공학회 2010 Structural Engineering and Mechanics, An Int'l Jou Vol.34 No.6

In order to consider high-order effects on the actual limit state function, a new response surface method is proposed for structural reliability analysis by the use of high-order approximation concept in this study. Hermite polynomials are used to determine the highest orders of input random variables, and the sampling points for the determination of highest orders are located on Gaussian points of Gauss-Hermite integration. The cross terms between two random variables, only in case that their corresponding percent contributions to the total variation of limit state function are significant, will be added to the response surface function to improve the approximation accuracy. As a result, significant reduction in computational cost is achieved with this strategy. Due to the addition of cross terms, the additional sampling points, laid on two-dimensional Gaussian points off axis on the plane of two significant variables, are required to determine the coefficients of the approximated limit state function. All available sampling points are employed to construct the final response surface function. Then, Monte Carlo Simulation is carried out on the final approximation response surface function to estimate the failure probability. Due to the use of high order polynomial, the proposed method is more accurate than the traditional second-order or linear response surface method. It also provides much more efficient solutions than the available high-order response surface method with less loss in accuracy. The efficiency and the accuracy of the proposed method compared with those of various response surface methods available are illustrated by five numerical examples.

대면적 미세가공을 위한 고주파 Fast Tool Servo 개발

Hong Lu,이승준(S. J. Lee),이상민(S. M. Lee),하양협(Y. H. Ha),박천홍(C. H. Park),이득우(D. W. Lee) 한국정밀공학회 2012 한국정밀공학회 학술발표대회 논문집 Vol.2012 No.5월

Application of Fast Tool Servo on Surface Texturing

Hong lu,S. J. Lee,J. M. Kim,S. H. Kim,J. K. Park,D. W. Lee 한국생산제조학회 2012 한국생산제조시스템학회 학술발표대회 논문집 Vol.2012 No.10

Molecular characterization of cDNA Encoding the ADP-Ribosylation Factor-like Protein (ARL), BcARF3, from Chinese Cabbage Flower Bud

Hong, Joon-Ki,Lee, Eun-Young,Jin, Zheng-Lu,Kim, Ho-Il,Hong, Jong-Chan,Choi, Young-Ju,Yun, Dae-Jin,Lee, Sang-Yeol,Bahk, Jeong-Dong,Cho, Moo-Je,Lim, Chae-Oh Plant molecular biology and biotechnology research 2002 Plant molecular biology and biotechnology research Vol.2002 No.-

A cDNA encoding a small GTP-binding protein highly similar to Arabidopsis ADP-ribosylation factor 3 (ARF3) was isolated from the Chinese cabbage flower bud cDNA library. The cDNA clone contained an open reading frame encoding a 182-amino acid polypeptide with a calculated molecular mass of 20.3 kDa. A multialignment and phylogenetic analysis demonstrated that all the plant genes evaluated were clustered in a major evolutionary tree branch, indicating the need for a new plant ADP-ribosylation factor (ARF) and ARF-like protein (ARL) subfamily classification. Several ARF or ARL genes were identified in Chinese cabbage genomic DNA by Southern analysis. ARL transcript levels, differentially expressed in different organs of the Chinese cabbage, were particularly high in the leaves. Accumulation of ARL transcript was nearly constant in seedlings treated with growth regulators. However, the ARL transcript was preferentially expressed in suspension culture cells following salicylic acid (SA) treatment but not following treatment with hydrogen peroxide (H_(2)o_(2)),jasmonic acid(JA), or nitric oxide (NO). Thus, a SA signaling pathway may regulate ARL expression in Chinese cabbage suspension culture cells.

Entrepreneurial Intention of Two Patterns of Planned Behaviour and Alertness: Empirical Evidence in China

Hong Lu,Jiafure Wang 한국유통과학회 2018 The Journal of Asian Finance, Economics and Busine Vol.5 No.2

Researches based on the pattern of planned behavior holds that the three variables of entrepreneurial attitudes, subjective norms, and perceived behavior control influence each other and influence entrepreneurial intentions respectively. However, there are also different, even conflicting research conclusions that continue to emerge. Researches based on the pattern of alertness, believe that profit opportunities and individuals’ pursuit to truth are the driving forces. Many scholars have demonstrated the impact of individual entrepreneurial alertness on entrepreneurial intentions. However, as an exogenous causal agent, profit opportunities have a logical problem: if there are no other assumptions, the mere existence of opportunities does not adequately explain entrepreneurial alertness. To address this gap, this study considered samples from mainland China, where entrepreneurial activities are very active currently, to test the role that entrepreneurial alertness is assumed to play in the planned behavior model. The results show that the three dimensions of alertness, individually partly intermediate the influence of entrepreneurial attitude on entrepreneurial intention, the influence of subjective norms on entrepreneurial intention, and that of perceived behavior control on entrepreneurial intention. This article studied the production of entrepreneurial intention by integrating the two patterns of planned behavior and alertness through empirical analysis, and opened up a new field for subsequent research on entrepreneurial intention.

Correlation of Adventitial Vasa Vasorum with Intracranial Atherosclerosis: A Postmortem Study

Lu Zheng,Wen Jie Yang,Chun Bo Niu,Hai Lu Zhao,Ka Sing Wong,Thomas Wai Hong Leung,Xiang Yan Chen 대한뇌졸중학회 2018 Journal of stroke Vol.20 No.3

Background and Purpose Vasa vasorum (VV) have been believed to be rare or non-existent in small-caliber intracranial arteries. In a series of human cerebral artery specimens, we identified and examined the distribution of VV in association with co-existing intracranial atherosclerosis. Methods We obtained cerebral artery specimens from 32 consecutive autopsies of subjects aged 45 years or above. We scrutinized middle cerebral artery (MCA), vertebral artery (VA), and basilar artery (BA) for the presence of adventitial VV. We described the distribution of VV, and the characteristics of co-existing atherosclerotic lesions. Results Among 157 intracranial arteries, adventitial VV were present in 74 of the 157 specimens (47%), involving MCA (n=13, 18%), BA (n=14, 19%), and VA (n=47, 64%). Although qualitatively these 74 adventitial VV distributed similarly in arteries with or without atherosclerotic lesions (disease-free arteries n=4/8; arteries of pre-atherosclerosis n=17/42; and arteries of progressive atherosclerosis n=53/107), the presence of adventitial VV in intracranial VA was associated with a heavier plaque load (1.72±1.66 mm2 vs. 0.40±0.32 mm2, P<0.001), severer luminal stenosis (25%±21% vs. 12%±9%, P=0.002), higher rate of concentric lesions (79% vs. 36%, P=0.002), and denser intraplaque calcification (44% vs. 0%, P=0.003). Histologically, intracranial VA with VV had a larger diameter (3.40±0.79 mm vs. 2.34±0.58 mm, P<0.001), thicker arterial wall (0.31±0.13 mm vs. 0.23±0.06 mm, P=0.002), and a larger intima-media (0.19±0.09 mm vs. 0.13± 0.04 mm, P=0.003) than VA without VV. Conclusions Our study demonstrated the distribution of adventitial VV within brain vasculature and association between vertebral VV and progressive atherosclerotic lesions with a heavier plaque load and denser intraplaque calcification.

말레이시아 공포영화 <두쿤>으로 본 남성의 시선, 여성의 유혹 그리고 영화촬영술

( Lu Hong ) 부산대학교 영화연구소 2021 아시아영화연구 Vol.14 No.1

본 논문은 말레이시아의 공포영화 <두쿤>에 나오는 여성의 유혹을 고찰하고 영화 속 말레이시아 사회에서 여성의 주체성과 대비되는 남성의 취약성을 살펴보고자 한다. 흥미로운 것은 여성의 주체성 권한이 여성을 향한 남성의 시선에서 비롯된다는 것이다. 영화 <두쿤>에서는 다소 모호하게 연출되었지만, 영화는 여전히 여성에 대한 남성의 시선을 역전시키려는 시도를 한다. 유혹적인 여성의 이미지는 주인공 다이애나의 흑마법을 통하여 남성들을 향해 여성의 시선을 던짐으로써 역전된다. 또한, 이 영화는 여성이 매력을 발휘할 수 있는 공간을 통해 가부장적인 사회에서 타자화된 여성 관객들의 공감을 이끌어낸다. 그럼에도 불구하고 흑마법을 통해 여성 권력이 결국에는 현대 사회의 가부장적 질서를 강화함으로써 여성의 권리는 약화되는 양상을 보인다. 그밖에 본 논문은 영화의 장면배치와 시각적인 은유를 통해 양성의 불평등한 권력 관계 및 주인공의 심리 표현을 분석하고자 한다. This paper mainly intends to examine the female seduction embedded in Malaysian film Dukun, and further explores the construction of female subjectivity articulated in this cinematic text, in comparison with the representation of vulnerability and precariousness of male characters in modern Malay society. Interestingly, the empowerment of female sexuality is initiated from the conventional male gaze towards female; while this film Dukun, though with ambiguity, I argue is attempting to make a reversal of male gaze towards female. Female seduction seems to disable male gaze. And this film has given way for feminine to play out its charm for identifications of female audiences who are being situated in the position of the other as to men in a mainly patriarchal society. Nevertheless, the female power presented through black magic ambivalently undermines itself in a way to reinforce the patriarchal order in modern society. Besides, the mise en scène and visual metaphors will be analyzed and encoded in representing characters’ psychological status as well as the dominant power one over the other in relation to its thematic concerns.

Development of a magnified flexure mechanism for fast tool servo system

Lu Hong(여굉),Soo Chang Choi(최수창),Yong Woo Kim(김용우),Jin Geun Kim(김진근),Deug Woo Lee(이득우) 한국생산제조학회 2009 한국공작기계학회 추계학술대회논문집 Vol.2009 No.-

The flexure-hinge mechanisms are commonly used in the design of translational micro/nano-positioning stage. This paper presents the methodology for design and control of a lever-type magnified flexure mechanism used for the ultra-precision fast tool servo (FTS) system. A lever type hinge mechanism is designed and utilized to guide the tool stage and to preload the PZT actuator. A prototype of the FTS system was developed and tested. The influence of tile PZT actuator's capacitance on the output voltage of the given amplifier was investigated. The motion range of the FTS system can reach 11.3㎛ and a bandwidth of 600㎐, and the amplification of the lever flexure mechanism is approximately 5 as calculated from the experiment.